The invention discloses
graphene oxide immobilized lipase as well as a preparation method and application thereof in preparation of
diglyceride oil, and the preparation method comprises the following steps: dissolving
graphene oxide powder in a
phosphate buffer solution, and carrying out ultrasonic treatment and
centrifugation to obtain pretreated
graphene oxide; the preparation method comprises the following steps: adding pretreated graphene oxide and
lipase into a
phosphate buffer solution for primary incubation, then adding a cross-linking agent for secondary incubation, and carrying out
centrifugal separation, precipitate washing, secondary
centrifugal separation and precipitate
drying to obtain the graphene oxide
immobilized lipase. According to the preparation method, the stability of the
lipase and the yield of
diglyceride oil are improved, the cost is reduced, the prepared graphene oxide
immobilized lipase can be applied to preparation of the
diglyceride oil, the content of diglyceride in the obtained product is larger than or equal to 50 wt%, the content of
monoglyceride is smaller than or equal to 1.5 wt%, the content of free
fatty acid is smaller than or equal to 0.5 wt%, and the yield of the diglyceride oil is increased. The
enzyme activity is greater than or equal to 90% after the graphene oxide immobilized
lipase is repeatedly used for more than 100 times.