The invention belongs to the technical field of sunflower seed oil processing, and particularly relates to a method for obtaining 1, 3-diglyceride from high oleic acid sunflower seed oil. The method for obtaining the 1, 3-diglyceride from the high-oleic-acid sunflower seed oil comprises the following steps: weighing the sunflower seed oil, adding candida antarctica lipase A and deionized water, carrying out enzymolysis, constant-temperature oscillation, centrifugation and secondary molecular distillation to finally obtain a product containing the 1, 3-diglyceride, and according to the weight of the high-oleic-acid sunflower seed oil, adding the 1, 3-diglyceride into the sunflower seed oil to obtain the 1, 3-diglyceride. The dosage of the candida antarctica lipase A is 2500-3500 u/g. According to the method, sunflower seed oil is used as a main raw material, the process operation is simple, and the product is rich in nutrition and high in economic value and has wide application prospects in the industries of food, medicine, chemical engineering and the like.