18 results about "Candida antarctica" patented technology

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The application provides a method, application and product for preparing a triglyceride oil with five effects of improving blood vessel health by enzymolysis, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, performing enzymolysis reaction, performing molecular distillation, and performing decolorization and deodorization to obtain the triglyceride oil; the lipase is a mixture of Candida antarctica black Aspergillus niger lipase, Candida cylindracea lipase and bovine pancreas lipase; and the weight ratio of the low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase is 500:80-100:1-2:30-60:10-20. The triglyceride oil prepared by the method can be used to prepare a triglyceride oil composition, and has the five effects of lowering blood uric acid, lowering blood total cholesterol, lowering blood triglyceride, lowering blood sugar and improving blood flow speed, thereby improving blood vessel health.

The invention discloses a method for rapidly detecting dichlorvos on site based on scattering turbidity, and belongs to the technical field of food and agricultural product safety detection. The method comprises the following steps: incubating dichlorvos with different concentrations with candida antarctica lipase, a phosphate buffer solution and L-ascorbyl palmitate, centrifugally incubating to obtain a reaction solution, taking a supernatant to react with a manganese dioxide nanosheet, measuring the turbidity of the reaction solution through a portable turbidimeter, and establishing a linear equation of the concentration and the turbidity of the dichlorvos; by measuring the turbidity of an unknown sample, the concentration of the dichlorvos can be calculated according to a linear equation. According to the method, invisible dichlorvos concentration signals are converted into measurable turbidity signals by utilizing the synergistic effect of enzyme inhibition reaction and turbidity change induction of the nano material, so that rapid and sensitive detection of dichlorvos is realized. The method is easy and convenient to operate, low in cost, high in detection speed, free of large instruments and especially suitable for on-site rapid screening of agricultural products.

The present application relates to the technical field of enzyme synthesis of target compounds, and particularly relates to a method for preparing diglyceride vegetable oil by enzyme method, which comprises the following steps: mixing vegetable oil and glycerol according to a mass ratio of 3:1, then adding 5%-10% of a composite enzyme resin to the mixed liquid in terms of the mass of the vegetable oil, oscillating and treating at 40-60 DEG C for 8-16h, and then performing solid-liquid separation to obtain the diglyceride vegetable oil; wherein the composite enzyme resin takes macroporous resin AB-8 as a carrier, the carrier pores are fixed with partial glyceride lipase, the carrier exterior is coated with a gel layer, and the gel layer is dispersed with Candida antarctica lipase A. The method for preparing the diglyceride vegetable oil by enzyme method provided by the present application shortens the original method for obtaining the diglyceride vegetable oil crude product in steps to one step, belongs to a one-pot operation, and the diglyceride content of the diglyceride vegetable oil produced finally is high.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

The present invention relates to a process for producing mono-acylglycerides (MAG) and / or di-acylglycerides (DAG) comprising steps of: a. providing a mixture comprising free fatty acids (FFA) and / or fatty acid alkyl esters, glycerol and lipase; b. reacting the mixture of a) to produce a mixture of MAG and DAG wherein the lipase is CalB from Candida antarctica which was found to produce MAG and DAG but almost no triacylglycerides using FFA and glycerol.

The invention relates to the technical field of functional lipid preparation, in particular to high-purity microalgae glyceroglycolipid enriched by an enzymic method and a preparation method of the high-purity microalgae glyceroglycolipid. The invention provides high-purity microalgae glyceroglycolipid enriched by an enzymic method and a preparation method of the high-purity microalgae glyceroglycolipid, triglyceride and phospholipid are selectively hydrolyzed by using substrate specificity of candida antarctica lipase B, but glyceroglycolipid components which cannot be hydrolyzed are retained and enriched, the glyceroglycolipid is retained to the maximum extent, and the yield of the microalgae is increased. The invention provides a method which is mild in reaction condition, environment-friendly, simple in step and high in purity for efficient enrichment of the microalgae glyceroglycolipid.

The invention provides a strain for producing candida antarctica lipase B as well as a construction method and application of the strain. The construction method comprises the following steps: S1, constructing a fusion expression cassette containing an MBP gene, a connection sequence and a CALB gene, the connection sequence containing a blood coagulation factor Xa enzyme recognition site; s2, inserting the fusion expression cassette into an expression vector to obtain a recombinant expression vector; and S3, transforming the recombinant expression vector into bacillus subtilis to obtain a recombinant strain. According to the invention, bacillus subtilis is used as a chassis bacterium, so that directional transformation and screening of strains can be rapidly realized, the yield of CALB can be increased, and the cost of industrial production can be reduced.

The application discloses a lipase A mutant and a preparation method and application thereof, and belongs to the technical field of biology. The lipase A mutant is a protein obtained by changing one or more amino acid residues at at least one of the following positions: 94th, 117th, 121st, 122nd, 283rd and 431st in the amino acid sequence shown in SEQ ID NO:1; wherein the change is selected from at least one of substitution, deletion and addition. The application performs site-directed mutation on the lipase A derived from Candida antarctica by rational design, obtains a Candida antarctica lipase A mutant with significantly improved catalytic activity and selectivity to the sn-2 site of triglyceride, and the Candida antarctica lipase A mutant can significantly improve the yield of 1,3-glycerol diester when preparing the 1,3-glycerol diester.

The invention discloses an acid-resistant mutant of candida antarctica lipase A and application of the acid-resistant mutant. In order to solve the problem that the Candida antarctica lipase A is easy to inactivate in an acid environment, the acid-resistant mutant of the Candida antarctica lipase A is obtained by mutating the 45th or 161th amino acid of the Candida antarctica lipase A. Compared with candida antarctica lipase A, the acid-resistant mutant has the advantages that the optimum reaction temperature, pH and specific activity of the acid-resistant mutant are not obviously changed, but the residual enzyme activity of the acid-resistant mutant is obviously improved after the acid-resistant mutant is incubated for the same time in an acid environment and is improved by 10-26%, and the yield of biodiesel prepared by catalyzing acid oil within the same time is also obviously improved. The mutant disclosed by the invention can be used for preparing biodiesel under an acidic condition, and is beneficial to recycling of acid oil.

The application belongs to the technical field of functional oil processing, and particularly relates to an enzymatic preparation method and application of a diglyceride with the effect of improving Alzheimer's disease. The method comprises the following steps: mixing low erucic acid rapeseed oil, glycerol and water, heating to obtain a pre-reaction mixture; adding a composite enzyme to react, then adding Candida antarctica lipase B and Pseudomonas fluorescens lipase to react; after the reaction is completed, performing molecular distillation to collect a light phase product, performing adsorption and filtration to collect a filtrate, and obtaining a diglyceride oil. The composite enzyme is composed of Mucor circinelloides lipase, Candida rugosa lipase and Candida antarctica lipase B with a weight ratio of 2-4:2:1. The method effectively improves the content of the diglyceride, and the prepared composition has the effect of preventing and treating Alzheimer's disease, which is manifested in the improvement of reaction ability and cognitive function, the inhibition of brain apoptosis and acetylcholinesterase, the enhancement of SOD and CAT activity, and the improvement of related gene expression.

The present invention relates to a method for producing acylglycerol mono- (MAG) and / or di-esters (DAG), said method comprising the steps of: a. providing a mixture comprising free fatty acids (FFA) and / or fatty acid alkyl esters, glycerol and a lipase; b. reacting the mixture of a) to produce a mixture of MAG and DAG, wherein the lipase is CalB from Candida antarctica, which has been found to produce MAG and DAG using FFA and glycerol, but almost no acylglycerol tri-esters.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition. The process comprises a) hydrolysis of the glyceride by Thermomyces lanuginosus lipase, b) separation of the free saturated fatty acids from the glycerides and c) enzymatic esterification of the hydrolyzed glyceride by Candida antarctica lipase B with a polyunsaturated fatty acid.

The invention belongs to the technical field of organic synthesis, and particularly discloses sec-octyl nitrate based on biomass raw materials, a preparation method and application of the sec-octyl nitrate and a system for the preparation method of the sec-octyl nitrate. The preparation method comprises the following steps: firstly, carrying out biological fermentation reaction on castor oil and mixed fermentation liquor to obtain an intermediate product containing sec-octanol; sequentially carrying out hydrogenation reaction and nitration reaction on the intermediate product containing sec-octyl alcohol to obtain sec-octyl nitrate; the mixed fermentation liquor comprises saccharomyces rouxii, pichia pastoris, saccharomyces cerevisiae, candida antarctica lipase, hexokinase and pyruvate kinase. The invention provides a new generation of cetane number improver, namely sec-octyl nitrate, the problem of serious pollution in the existing cetane number improver production process is avoided, and low-carbon environment-friendly preparation of the cetane number improver is realized.

The present invent ion concerns a method for preparing a fatty amidoalkyldialkylamine by reacting a fatty acid with a dialkylaminoalkylamine, using a molar ratio of said dialkylaminoalkylamine to said fatty acid of more than 1 and up to 1.5, in the presence of Candida antarctica lipase as catalyst.

The present invention relates to a process for producing mono-acylglycerides (MAG) and / or di-acylglycerides (DAG) comprising steps of: a. providing a mixture comprising free fatty acids (FFA) and / or fatty acid alkyl esters, glycerol and lipase; b. reacting the mixture of a) to produce a mixture of MAG and DAG wherein the lipase is CalB from Candida antarctica which was found to produce MAG and DAG but almost no triacylglycerides using FFA and glycerol.