36 results about "Candida antarctica" patented technology

The present invention relates to the field of food technology, and in particular to a method for preparing diglyceride and its application. The method comprises the following steps: firstly hydrolyzing the oil and fat under the action of a first lipase, adding a solubilizing agent, and obtaining a hydrolyzate; then mixing the hydrolyzate with glycerol, and performing an esterification reaction with a second lipase; wherein the first lipase is prepared by compounding lipase Lipozyme RM and Antarctic Candida lipase B. The present invention can specifically synthesize 1,3-diglyceride that is beneficial to the cardiovascular system of the body, and has a high yield and good application prospects.

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

The invention provides a method for preparing quintuple diglyceride oil for improving blood vessel health through enzymolysis, application and a product, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, and carrying out enzymolysis reaction, molecular distillation, decoloration and deodorization to obtain diglyceride oil; the lipase is a mixture of Candida antarctica Aspergillus niger lipase, Candida column lipase and bovine pancreas lipase, and the weight ratio of the low-erucic acid rapeseed oil to the glycerin to the water to the fatty acid to the lipase is 500: (80-100): (1-2): (30-60): (10-20). The diglyceride oil prepared by the method can be used for preparing a diglyceride oil composition, and has five functions of reducing blood uric acid, reducing blood total cholesterol, reducing blood triglyceride, reducing blood sugar and increasing blood flow velocity to improve blood vessel health.

The invention discloses a lipase A mutant as well as a preparation method and application thereof, and belongs to the technical field of biology. The lipase A mutant is a protein obtained by carrying out one or more amino acid residue changes on at least one of the following sites of an amino acid sequence as shown in SEQ ID NO: 1: the 94th site, the 117th site, the 121st site, the 122nd site, the 283rd site and the 431st site; wherein the change is selected from at least one of substitution, deletion and addition. Through rational design, lipase A from Candida antarctica is subjected to site-directed mutagenesis, the Candida antarctica lipase A mutant with catalytic activity and selectivity to the sn-2 site of triglyceride remarkably improved is obtained, and when 1, 3-diglyceride is prepared, the Candida antarctica lipase A mutant can remarkably improve the yield of 1, 3-diglyceride. The yield of the 1, 3-diglyceride is improved.

The invention provides diglyceride oil with a blood sugar reducing effect and a preparation method of the diglyceride oil, and relates to the technical field of functional grease processing. The preparation method comprises the following steps: mixing sea buckthorn seed oil, acer truncatum seed oil, rapeseed oil, corn oil and high oleic acid peanut oil, and adding glycerol and water to obtain a material A; adding onion pseudomonas lipase and candida antarctica lipase B into the material A, and carrying out primary reaction to obtain a material B; adding candida lipolytica lipase and candida antarctica lipase B into the material B, and carrying out a second reaction to obtain a material C; adding thermomyces lanuginosus lipase into the material C, and carrying out a third reaction to obtain a material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the invention has a good effect in the aspect of reducing blood sugar.

The invention relates to the technical field of biocatalytic synthesis of fatty glyceride, in particular to a flaky zeolite immobilized Candida antarctica lipase B catalyst and a method for preparing medium-chain glyceride through catalytic esterification of the flaky zeolite immobilized Candida antarctica lipase B catalyst. The flaky zeolite is flaky silica-alumina zeolite ZSM-5-P, and the loading capacity of the candida antarctica lipase B is 9-12% of the mass of the flaky zeolite. The candida antarctica lipase B is loaded and fixed in the flaky zeolite to prepare the solid catalyst, and the catalyst is a solid enzyme biocatalyst, is small in usage amount, high in catalytic efficiency and recyclable, is used for preparing the medium-chain glyceride, is high in efficiency, low in cost, simple in process, mild in condition, safe and environment-friendly, and has a wide application prospect.

The invention discloses diglyceride prepared by an enzymic method as well as a preparation method and application thereof, and belongs to the technical field of deep processing of grease. The method comprises the following steps: adding fatty acid into a choline chloride-glycerol-urea ternary eutectic solvent, uniformly mixing, and then adding candida antarctica lipase B for esterification reaction to obtain a reaction mixture; separating the reaction mixture, and recovering an upper-layer oil phase; and separating and purifying the upper-layer oil phase to obtain the diglyceride. According to the method, the reaction rate is high, the selectivity of the diglyceride in the esterification process is high, the yield of the diglyceride can reach 65% or above by reacting for 1 h, the selectivity of the diglyceride is 75% or above, and the purity of the separated and purified diglyceride is 90% or above; in addition, the method is simple to operate, and the reaction product and the eutectic solvent can be separated through simple centrifugation after the reaction is finished; in addition, the monoglyceride content in the product is low, and the subsequent separation and purification difficulty is greatly reduced.

The present invention provides a method, application and product for preparing diglyceride with weight loss effect by enzymolysis, which belongs to the field of enzyme engineering technology. The method comprises the following steps: S1, mixing lipase, ammonium heptamolybdate tetrahydrate with water, adding glycerol precipitation, filtering, and obtaining an enzyme preparation; S2, mixing plant raw material oil, glycerol, fatty acid, sodium fatty acid with the enzyme preparation obtained in step S1, reacting under inert gas conditions, and obtaining an enzymolysate; S3, molecularly distilling the enzymolysate obtained in step S2 to obtain diglyceride; wherein the lipase is a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase. The present invention uses ammonium heptamolybdate tetrahydrate to load a specific lipase mixture, and at the same time optimizes the component types and ratios of the raw materials in the enzymolysis process, effectively improving the mass percentage content and diglyceride yield of enzymolysis production of diglyceride.

Disclosed is a method of reducing and / or removing diglyceride content in an oil without substantial transesterification of triglycerides, the method comprising the steps of: providing an oil or fat; and hydrolyzing the monoglycerides and diglycerides in the oil with water in the presence of a lipase having at least 80% sequence identity to SEQ ID NO: 1.

The application provides a method, application and product for preparing a triglyceride oil with five effects of improving blood vessel health by enzymolysis, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mixing low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase, performing enzymolysis reaction, performing molecular distillation, and performing decolorization and deodorization to obtain the triglyceride oil; the lipase is a mixture of Candida antarctica black Aspergillus niger lipase, Candida cylindracea lipase and bovine pancreas lipase; and the weight ratio of the low erucic acid rapeseed oil, glycerol, water, fatty acid and lipase is 500:80-100:1-2:30-60:10-20. The triglyceride oil prepared by the method can be used to prepare a triglyceride oil composition, and has the five effects of lowering blood uric acid, lowering blood total cholesterol, lowering blood triglyceride, lowering blood sugar and improving blood flow speed, thereby improving blood vessel health.

The invention relates to the technical field of enzyme engineering, and discloses a preparation method for improving acid resistance and temperature resistance of an enzyme preparation, which comprises the following steps: step 1, selecting enzyme sources: selecting candida antarctica, serratia liquefaciens and pseudomonas pseudoalcaligenes as the enzyme sources; step 2, selecting an additive: adding the additive into the enzyme source; 3, culturing an enzyme source: selecting a culture medium for culturing, and setting the culture temperature and pH value range of the enzyme preparation; step 4, extracting an enzyme solution, namely breaking cells by using an enzymolysis method, and extracting the enzyme solution; 5, quality detection: detecting enzyme activity, acid resistance and temperature resistance; and step 6, packaging and storing: sealing and packaging the enzyme preparation which is qualified in quality detection by adopting a sterile container, regulating the temperature and Ph in a specified range according to a specified temperature condition and a specified Ph range, and preparing the enzyme preparation in three stages, so that the enzyme activity degree is still high after temperature gradient test and pH gradient test experiments.

The invention discloses a method for rapidly detecting dichlorvos on site based on scattering turbidity, and belongs to the technical field of food and agricultural product safety detection. The method comprises the following steps: incubating dichlorvos with different concentrations with candida antarctica lipase, a phosphate buffer solution and L-ascorbyl palmitate, centrifugally incubating to obtain a reaction solution, taking a supernatant to react with a manganese dioxide nanosheet, measuring the turbidity of the reaction solution through a portable turbidimeter, and establishing a linear equation of the concentration and the turbidity of the dichlorvos; by measuring the turbidity of an unknown sample, the concentration of the dichlorvos can be calculated according to a linear equation. According to the method, invisible dichlorvos concentration signals are converted into measurable turbidity signals by utilizing the synergistic effect of enzyme inhibition reaction and turbidity change induction of the nano material, so that rapid and sensitive detection of dichlorvos is realized. The method is easy and convenient to operate, low in cost, high in detection speed, free of large instruments and especially suitable for on-site rapid screening of agricultural products.

The present invention provides a diglyceride oil with hypoglycemic effect and a preparation method thereof, which relates to the technical field of functional oil processing. The preparation method comprises the following steps: mixing seabuckthorn seed oil, Acer truncatum Bunge seed oil, rapeseed oil, corn oil and high-oleic acid peanut oil, adding glycerol and water to obtain material A; adding Pseudomonas cepacia lipase and Candida antarctica lipase B to material A for a primary reaction to obtain material B; adding Candida lipolytica lipase and Candida antarctica lipase B to material B for a second reaction to obtain material C; adding Thermomyces lanuginosus lipase to material C for a third reaction to obtain material D; and treating the reacted material D to obtain the diglyceride oil. The diglyceride oil prepared by the present invention has a good effect in reducing blood sugar.

The invention belongs to the technical field of ascorbic acid derivative synthesis, and particularly relates to a synthesis process of VC derivative ascorbic acid glyceride. Comprising the following steps: S1, co-loading candida antarctica lipase and glycosyltransferase into hierarchical pore silicon dioxide nanospheres, then dispersing the hierarchical pore silicon dioxide nanospheres into a eutectic solvent, and uniformly dispersing to obtain a reaction system; s2, adding vitamin C and glycerol into the reaction system, heating to 40-50 DEG C, and stirring to react for 10-12 hours; and S3, after the reaction is finished, collecting a product, and recovering the hierarchical pore silicon dioxide nanospheres. According to the method, the limitation of traditional chemical synthesis and single-enzyme catalysis is broken through, and stable ascorbyl glyceride with high antioxidant activity is efficiently synthesized by combining double-enzyme synergistic catalysis with the confinement reaction of the hierarchical porous silicon dioxide nanospheres and a special eutectic solvent system; and a theoretical basis is provided for a process for preparing ascorbyl glyceride through double-enzyme synergy.

The invention belongs to the technical field of biological enzymes, and particularly relates to a preparation method of immobilized lipase and application of the immobilized lipase in catalysis of oil hydrolysis, and the preparation method comprises the following steps: step 1, material selection: selecting Candida antarctica lipase A, porous ceramic and a phosphate buffer; according to the preparation method of the immobilized lipase and the application of the immobilized lipase in catalysis of grease hydrolysis, porous ceramic is adopted as an immobilization carrier, and candida antarctica lipase A is immobilized; the obtained immobilized lipase is used for catalyzing a grease hydrolysis reaction, and the acid value of a product obtained after the grease hydrolysis reaction is catalyzed can reach 180-195 mgKOH / g. Compared with free enzyme, the immobilized enzyme has the advantages that the catalytic activity of the enzyme is obviously improved under the same condition; in addition, the immobilized enzyme has high thermal stability and pH tolerance; the immobilized enzyme is good in reusability and convenient to recycle.

The present application relates to the technical field of enzyme synthesis of target compounds, and particularly relates to a method for preparing diglyceride vegetable oil by enzyme method, which comprises the following steps: mixing vegetable oil and glycerol according to a mass ratio of 3:1, then adding 5%-10% of a composite enzyme resin to the mixed liquid in terms of the mass of the vegetable oil, oscillating and treating at 40-60 DEG C for 8-16h, and then performing solid-liquid separation to obtain the diglyceride vegetable oil; wherein the composite enzyme resin takes macroporous resin AB-8 as a carrier, the carrier pores are fixed with partial glyceride lipase, the carrier exterior is coated with a gel layer, and the gel layer is dispersed with Candida antarctica lipase A. The method for preparing the diglyceride vegetable oil by enzyme method provided by the present application shortens the original method for obtaining the diglyceride vegetable oil crude product in steps to one step, belongs to a one-pot operation, and the diglyceride content of the diglyceride vegetable oil produced finally is high.

The invention relates to the technical field of enzyme immobilization, and particularly discloses a method for coupling and immobilizing lipase by a ligand. The method comprises the following steps: synthesizing a metal salt precursor of Co-MOF by taking cobaltous sulfate heptahydrate as a soft template, dissolving cobaltous sulfate heptahydrate and horse radish peroxidase in a proper amount of methanol to form colloidal agglomerated pellets, carrying out a coordination reaction with trimesic acid, dissolving the soft template through a water phase, and carrying out pore forming to obtain the ligand-immobilized lipase. According to the method, HRP (at) Me-Co-MOF is taken as a substrate, HRP (at) Me-Co-MOF is obtained, immobilization of horse radish peroxidase is realized, immobilization of Candida antarctica lipase is realized through a covalent linkage method, and finally the ligand-coupled immobilized lipase is prepared. The ligand-coupled immobilized lipase prepared by the invention has the characteristics of being capable of promoting efficient synthesis of quinoxaline compounds and being repeatedly used.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

The present invention relates to a process for producing mono-acylglycerides (MAG) and / or di-acylglycerides (DAG) comprising steps of: a. providing a mixture comprising free fatty acids (FFA) and / or fatty acid alkyl esters, glycerol and lipase; b. reacting the mixture of a) to produce a mixture of MAG and DAG wherein the lipase is CalB from Candida antarctica which was found to produce MAG and DAG but almost no triacylglycerides using FFA and glycerol.

The invention relates to the technical field of functional lipid preparation, in particular to high-purity microalgae glyceroglycolipid enriched by an enzymic method and a preparation method of the high-purity microalgae glyceroglycolipid. The invention provides high-purity microalgae glyceroglycolipid enriched by an enzymic method and a preparation method of the high-purity microalgae glyceroglycolipid, triglyceride and phospholipid are selectively hydrolyzed by using substrate specificity of candida antarctica lipase B, but glyceroglycolipid components which cannot be hydrolyzed are retained and enriched, the glyceroglycolipid is retained to the maximum extent, and the yield of the microalgae is increased. The invention provides a method which is mild in reaction condition, environment-friendly, simple in step and high in purity for efficient enrichment of the microalgae glyceroglycolipid.

The invention provides a strain for producing candida antarctica lipase B as well as a construction method and application of the strain. The construction method comprises the following steps: S1, constructing a fusion expression cassette containing an MBP gene, a connection sequence and a CALB gene, the connection sequence containing a blood coagulation factor Xa enzyme recognition site; s2, inserting the fusion expression cassette into an expression vector to obtain a recombinant expression vector; and S3, transforming the recombinant expression vector into bacillus subtilis to obtain a recombinant strain. According to the invention, bacillus subtilis is used as a chassis bacterium, so that directional transformation and screening of strains can be rapidly realized, the yield of CALB can be increased, and the cost of industrial production can be reduced.

The invention relates to the technical field of enzymatic synthesis of a target compound, in particular to a method for preparing diglyceride vegetable oil by an enzymatic method, which comprises the following steps: mixing vegetable oil and glycerol according to a mass ratio of 3: 1, adding 5-10% of compound enzyme resin into the mixed material liquid according to the mass of the vegetable oil, oscillating at 40-60 DEG C for 8-16 hours, and performing solid-liquid separation after the oscillation treatment is finished, thereby obtaining the diglyceride vegetable oil. The diglyceride vegetable oil is obtained; wherein the compound enzyme resin takes macroporous resin AB-8 as a carrier, partial glyceride lipase is fixed in pores of the carrier, the carrier is coated with a gel layer, and candida antarctica lipase A is dispersed in the gel layer. According to the method for preparing the diglyceride vegetable oil through the enzyme method, the original method for obtaining the diglyceride vegetable oil crude product step by step is shortened to one step, the method belongs to one-pot operation, and the diglyceride content of the finally produced diglyceride vegetable oil is high.

The present invention provides a diglyceride oil composition for reducing lactose, a product thereof, and a preparation method thereof, and relates to the technical field of functional oil processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high-oleic peanut oil, adding glycerol and water, and then adding Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and reacting to obtain material A; (2) mixing seabuckthorn seed oil, Acer truncatum seed oil, and rapeseed oil, adding glycerol and water, and then adding Candida antarctica lipase B and Rhizopus oryzae lipase, and reacting to obtain material B; (3) mixing material A and material B, heating and centrifuging, taking an upper oil sample, and distilling under vacuum conditions to obtain a diglyceride oil composition. The diglyceride oil composition prepared by the present invention has good efficacy in reducing lactose.

The application discloses a lipase A mutant and a preparation method and application thereof, and belongs to the technical field of biology. The lipase A mutant is a protein obtained by changing one or more amino acid residues at at least one of the following positions: 94th, 117th, 121st, 122nd, 283rd and 431st in the amino acid sequence shown in SEQ ID NO:1; wherein the change is selected from at least one of substitution, deletion and addition. The application performs site-directed mutation on the lipase A derived from Candida antarctica by rational design, obtains a Candida antarctica lipase A mutant with significantly improved catalytic activity and selectivity to the sn-2 site of triglyceride, and the Candida antarctica lipase A mutant can significantly improve the yield of 1,3-glycerol diester when preparing the 1,3-glycerol diester.

The invention discloses an acid-resistant mutant of candida antarctica lipase A and application of the acid-resistant mutant. In order to solve the problem that the Candida antarctica lipase A is easy to inactivate in an acid environment, the acid-resistant mutant of the Candida antarctica lipase A is obtained by mutating the 45th or 161th amino acid of the Candida antarctica lipase A. Compared with candida antarctica lipase A, the acid-resistant mutant has the advantages that the optimum reaction temperature, pH and specific activity of the acid-resistant mutant are not obviously changed, but the residual enzyme activity of the acid-resistant mutant is obviously improved after the acid-resistant mutant is incubated for the same time in an acid environment and is improved by 10-26%, and the yield of biodiesel prepared by catalyzing acid oil within the same time is also obviously improved. The mutant disclosed by the invention can be used for preparing biodiesel under an acidic condition, and is beneficial to recycling of acid oil.

The application belongs to the technical field of functional oil processing, and particularly relates to an enzymatic preparation method and application of a diglyceride with the effect of improving Alzheimer's disease. The method comprises the following steps: mixing low erucic acid rapeseed oil, glycerol and water, heating to obtain a pre-reaction mixture; adding a composite enzyme to react, then adding Candida antarctica lipase B and Pseudomonas fluorescens lipase to react; after the reaction is completed, performing molecular distillation to collect a light phase product, performing adsorption and filtration to collect a filtrate, and obtaining a diglyceride oil. The composite enzyme is composed of Mucor circinelloides lipase, Candida rugosa lipase and Candida antarctica lipase B with a weight ratio of 2-4:2:1. The method effectively improves the content of the diglyceride, and the prepared composition has the effect of preventing and treating Alzheimer's disease, which is manifested in the improvement of reaction ability and cognitive function, the inhibition of brain apoptosis and acetylcholinesterase, the enhancement of SOD and CAT activity, and the improvement of related gene expression.

The present invention relates to a method for producing acylglycerol mono- (MAG) and / or di-esters (DAG), said method comprising the steps of: a. providing a mixture comprising free fatty acids (FFA) and / or fatty acid alkyl esters, glycerol and a lipase; b. reacting the mixture of a) to produce a mixture of MAG and DAG, wherein the lipase is CalB from Candida antarctica, which has been found to produce MAG and DAG using FFA and glycerol, but almost no acylglycerol tri-esters.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition. The process comprises a) hydrolysis of the glyceride by Thermomyces lanuginosus lipase, b) separation of the free saturated fatty acids from the glycerides and c) enzymatic esterification of the hydrolyzed glyceride by Candida antarctica lipase B with a polyunsaturated fatty acid.