11 results about "Candida antarctica" patented technology

The invention discloses a method for rapidly detecting dichlorvos on site based on scattering turbidity, and belongs to the technical field of food and agricultural product safety detection. The method comprises the following steps: incubating dichlorvos with different concentrations with candida antarctica lipase, a phosphate buffer solution and L-ascorbyl palmitate, centrifugally incubating to obtain a reaction solution, taking a supernatant to react with a manganese dioxide nanosheet, measuring the turbidity of the reaction solution through a portable turbidimeter, and establishing a linear equation of the concentration and the turbidity of the dichlorvos; by measuring the turbidity of an unknown sample, the concentration of the dichlorvos can be calculated according to a linear equation. According to the method, invisible dichlorvos concentration signals are converted into measurable turbidity signals by utilizing the synergistic effect of enzyme inhibition reaction and turbidity change induction of the nano material, so that rapid and sensitive detection of dichlorvos is realized. The method is easy and convenient to operate, low in cost, high in detection speed, free of large instruments and especially suitable for on-site rapid screening of agricultural products.

The present application relates to the technical field of enzyme synthesis of target compounds, and particularly relates to a method for preparing diglyceride vegetable oil by enzyme method, which comprises the following steps: mixing vegetable oil and glycerol according to a mass ratio of 3:1, then adding 5%-10% of a composite enzyme resin to the mixed liquid in terms of the mass of the vegetable oil, oscillating and treating at 40-60 DEG C for 8-16h, and then performing solid-liquid separation to obtain the diglyceride vegetable oil; wherein the composite enzyme resin takes macroporous resin AB-8 as a carrier, the carrier pores are fixed with partial glyceride lipase, the carrier exterior is coated with a gel layer, and the gel layer is dispersed with Candida antarctica lipase A. The method for preparing the diglyceride vegetable oil by enzyme method provided by the present application shortens the original method for obtaining the diglyceride vegetable oil crude product in steps to one step, belongs to a one-pot operation, and the diglyceride content of the diglyceride vegetable oil produced finally is high.

The invention relates to the technical field of medicinal chemistry, in particular to a preparation method of Corey lactone diol. The invention provides a preparation method of Corey lactone diol. The preparation method comprises the following steps: S1, carrying out enzymatic reaction on a compound as shown in a formula II under the action of lipase to obtain a compound as shown in a formula III; s2, hydrolyzing the compound shown in the formula III to obtain a compound shown in a formula I; the lipase is selected from at least one of Candida antarctica lipase, Pseudomonas fluorescens lipase, Burkholderia lipase, Rhizopus mirabilis lipase and porcine pancreatic lipase. The method has the advantages of mild and simple reaction conditions, cheap and easily available raw materials, few reaction byproducts, high chiral optical purity of the product, short overall reaction steps and suitability for industrial production, simplifies the subsequent preparation process of the prostaglandin drugs, and reduces the production cost.

The invention relates to the technical field of functional lipid preparation, in particular to high-purity microalgae glyceroglycolipid enriched by an enzymic method and a preparation method of the high-purity microalgae glyceroglycolipid. The invention provides high-purity microalgae glyceroglycolipid enriched by an enzymic method and a preparation method of the high-purity microalgae glyceroglycolipid, triglyceride and phospholipid are selectively hydrolyzed by using substrate specificity of candida antarctica lipase B, but glyceroglycolipid components which cannot be hydrolyzed are retained and enriched, the glyceroglycolipid is retained to the maximum extent, and the yield of the microalgae is increased. The invention provides a method which is mild in reaction condition, environment-friendly, simple in step and high in purity for efficient enrichment of the microalgae glyceroglycolipid.

The invention provides a strain for producing candida antarctica lipase B as well as a construction method and application of the strain. The construction method comprises the following steps: S1, constructing a fusion expression cassette containing an MBP gene, a connection sequence and a CALB gene, the connection sequence containing a blood coagulation factor Xa enzyme recognition site; s2, inserting the fusion expression cassette into an expression vector to obtain a recombinant expression vector; and S3, transforming the recombinant expression vector into bacillus subtilis to obtain a recombinant strain. According to the invention, bacillus subtilis is used as a chassis bacterium, so that directional transformation and screening of strains can be rapidly realized, the yield of CALB can be increased, and the cost of industrial production can be reduced.

The invention discloses an acid-resistant mutant of candida antarctica lipase A and application of the acid-resistant mutant. In order to solve the problem that the Candida antarctica lipase A is easy to inactivate in an acid environment, the acid-resistant mutant of the Candida antarctica lipase A is obtained by mutating the 45th or 161th amino acid of the Candida antarctica lipase A. Compared with candida antarctica lipase A, the acid-resistant mutant has the advantages that the optimum reaction temperature, pH and specific activity of the acid-resistant mutant are not obviously changed, but the residual enzyme activity of the acid-resistant mutant is obviously improved after the acid-resistant mutant is incubated for the same time in an acid environment and is improved by 10-26%, and the yield of biodiesel prepared by catalyzing acid oil within the same time is also obviously improved. The mutant disclosed by the invention can be used for preparing biodiesel under an acidic condition, and is beneficial to recycling of acid oil.

The present invention relates to a method for producing acylglycerol mono- (MAG) and / or di-esters (DAG), said method comprising the steps of: a. providing a mixture comprising free fatty acids (FFA) and / or fatty acid alkyl esters, glycerol and a lipase; b. reacting the mixture of a) to produce a mixture of MAG and DAG, wherein the lipase is CalB from Candida antarctica, which has been found to produce MAG and DAG using FFA and glycerol, but almost no acylglycerol tri-esters.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition. The process comprises a) hydrolysis of the glyceride by Thermomyces lanuginosus lipase, b) separation of the free saturated fatty acids from the glycerides and c) enzymatic esterification of the hydrolyzed glyceride by Candida antarctica lipase B with a polyunsaturated fatty acid.

The present invent ion concerns a method for preparing a fatty amidoalkyldialkylamine by reacting a fatty acid with a dialkylaminoalkylamine, using a molar ratio of said dialkylaminoalkylamine to said fatty acid of more than 1 and up to 1.5, in the presence of Candida antarctica lipase as catalyst.