Preparation method of enzyme-loaded xerogel monolithic column catalytic filler

A catalytic filler and xerogel technology, applied in biochemical equipment and methods, fixed on/in organic carriers, enzymes, etc., can solve the problems of reaction system pollution, reaction system complexity, post-processing difficulty, etc. Achieve the effect of good selectivity, high activity and fast reaction rate

Active Publication Date: 2021-05-07
HEBEI UNIV OF TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immobilized enzyme catalyst on the coating is easy to expose the active center of the enzyme, making the enzyme more likely to be inactivated. In addition, the coating i...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of enzyme-loaded xerogel monolithic column catalytic filler
  • Preparation method of enzyme-loaded xerogel monolithic column catalytic filler
  • Preparation method of enzyme-loaded xerogel monolithic column catalytic filler

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Weigh 0.58g of methyl orthosilicate and 2.08g of methyltrimethoxysilane, mix and dissolve them in 3.39g of methanol.

[0038] (2) Put the solution prepared in step (1) into an ice-water bath for later use.

[0039] (3) Weigh 0.14g polyethylene glycol, 0.49g NaF, 1.26g enzyme solution (Candida antarctica lipase B solution; concentration: 6wt%), 2.06g deionized water, mix and stir evenly.

[0040] (4) Slowly pour the enzyme-containing mixed solution obtained in step (3) into the mixed solution in step (1), and stir in an ice-water bath for 3 minutes to obtain an enzyme-loaded wet gel.

[0041] (5) Cut a plastic round tube with an inner diameter of 1 cm and a thickness of 2 mm into 1.7 cm long pieces, a total of 10 pieces, glue one side of the cut piece to a 5×6 cm plastic plate, and place it at 60°C After drying in an oven for 12 hours at a degree Celsius, a cylindrical mold was obtained.

[0042] (6) Transfer the enzyme-loaded wet gel obtained in step (4) to the cy...

Embodiment 2

[0046] The difference between this embodiment and embodiment 1 is that the opening ratio of the perforated holes on the plastic wrap is replaced by 8%, the temperature of the mold putting into the oven is replaced by 35°C, and other conditions are completely the same as in embodiment 1.

Embodiment 3

[0048] The difference between this embodiment and embodiment 1 is that the opening ratio of the perforated holes on the plastic wrap is replaced by 6%, the temperature of the mold being put into the oven is replaced by 25° C., and other conditions are completely the same as in embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of an enzyme-loaded xerogel monolithic column catalytic filler. The method comprises the following steps of embedding Candida antarctica lipase B through a sol-gel method, taking organosilicon as a precursor, carrying out hydrolysis and condensation polymerization, and carrying out normal-pressure drying by using a cylindrical mold to prepare the cylindrical enzyme-loaded xerogel monolithic column catalytic filler. In the obtained enzyme-loaded xerogel monolithic column catalytic filler, xerogel has a silicon network structure, enzyme is embedded in the xerogel, and the enzyme-loaded xerogel monolithic column catalytic filler has the advantages of complete catalysis, no surface crack, high mechanical strength and the like.

Description

technical field [0001] The present invention relates to a kind of loaded lipase CALB xerogel monolithic column catalytic filler biocatalyst with silicon network structure and its preparation and application method, in particular, relates to a method for synthesizing acetic acid by the transesterification reaction of ethyl acetate and n-butanol An n-butyl ester-loaded enzyme xerogel monolithic column catalytic filler biocatalyst and a preparation method thereof. Background technique [0002] N-butyl acetate, referred to as butyl acetate, is the most commonly used solvent and can be used as an excellent organic solvent. It is widely used in nitrocellulose varnishes, used as a solvent in the processing of artificial leather, fabrics and plastics, and also used in the perfume industry. Analytical reagents and extractants, chromatographic analysis standard substances and solvents, used in the production of paints and coatings, and can also be used in the fragrance industry. Ther...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N11/04C12N11/089C12N9/20C12P7/62
CPCC12N11/04C12N11/089C12N9/20C12P7/62C12Y301/01003Y02P20/584
Inventor 王洪海岳文达张淑玲魏斯文董凯王中彪
Owner HEBEI UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap