Biologic deodorant as well as preparation method and application thereof

A biological deodorant, biological fermentation technology, applied in the direction of biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of unsatisfactory biological deodorant effect, environmental pollution of chemical deodorant, etc. Production cycle, environmental friendliness, the effect of solving the problem of odor generation

Active Publication Date: 2014-07-02
重庆鸿紫圆光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a biological deodorant, which aims to solve the problem that

Method used

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  • Biologic deodorant as well as preparation method and application thereof
  • Biologic deodorant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of biological deodorant comprises the following steps:

[0042] (1) Mix Candida antarctica, Candida rugosa, Bacillus licheniformis and Bacillus subtilis in a ratio of 2:4:1:3 to obtain microbial strains, and use tea seed cake for the microbial strains as the sole nitrogen source for acclimatization.

[0043] (2) After inoculating the microbial strains domesticated in the step (1) into the liquid medium, adopt a two-stage fermentation method to cultivate, the first stage adopts a temperature of 24 ° C, a shaking table speed of 200 rpm, and after cultivating for 20 hours, Adjust the temperature to 38°C and continue to ferment for 36 hours;

[0044] In step (2), the specific composition of the liquid medium is: tea seed protein meal 25g / L, potato 32g / L, honey 5g / L, bee pollen 5g / L, yeast extract 2g / L, glucose 20g / L , Tween 0.01g / L, calcium chloride 0.3g / L, magnesium sulfate 0.1g / L, urea 3g / L, sodium chloride 5g / L, sodium nitrite 5g / L, ferrous sulfa...

Embodiment 2

[0047] The preparation method of biological deodorant comprises the following steps:

[0048] (1) Candida Antarctica and Bacillus licheniformis were mixed at a ratio of 2:1 to obtain microbial strains, and the microbial strains were domesticated using tea seed cake as the sole nitrogen source.

[0049] (2) After inoculating the microbial strains domesticated in the step (1) into the liquid medium, adopt a two-stage fermentation method to cultivate, the first stage adopts a temperature of 26°C, a shaker speed of 150rpm, and after cultivating for 20h, Adjust the temperature to 32°C and continue to ferment for 20 hours;

[0050] In step (2), the specific composition of the liquid medium is: tea seed protein meal 20g / L, potato 32g / L, honey 8g / L, bee pollen 3g / L, yeast extract 2.5g / L, glucose 10g / L L, Tween 0.08g / L, calcium chloride 0.12g / L, magnesium sulfate 0.3g / L, urea 2g / L, sodium chloride 8g / L, sodium nitrite 3g / L, ferrous sulfate 0.009g / L , dipotassium hydrogen phosphate 2g...

Embodiment 3

[0053] The preparation method of biological deodorant comprises the following steps:

[0054] (1) Candida antarctica, Bacillus licheniformis and Bacillus subtilis were mixed according to the bacterial ratio of 2:1:3 to obtain a microbial strain, and the microbial strain was domesticated with tea seed cake as the only nitrogen source.

[0055] (2) After inoculating the microbial strains domesticated in the step (1) into the liquid medium, adopt a two-stage fermentation method to cultivate, the first stage adopts a temperature of 25°C, a shaker speed of 180rpm, and after cultivating for 18h, Regulating temperature is 32~38 ℃ and continues to ferment for 20~36 hours.

[0056] In step (2), the specific composition of the liquid medium is: tea seed protein meal 8g / L, potato 32g / L, honey 10g / L, bee pollen 2g / L, yeast extract 3g / L, glucose 20g / L , Tween 0.1g / L, calcium chloride 0.1g / L, magnesium sulfate 0.5g / L, urea 3g / L, sodium chloride 5g / L, sodium nitrite 5g / L, ferrous sulfate 0....

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Abstract

The invention provides a biologic deodorant as well as a preparation method and an application thereof. The biologic deodorant is a biologic fermentation liquid of which a microorganism strain is combined with one or more of yeast strains or one or two of bacteria into a mixed bacterium, wherein the yeast strains comprise candida Antarctica lipase and candida rugosa; the bacteria comprise bacillus licheniformis and bacillus subtilis; after microorganism strains are subjected to strain domesticating by using tea seed cake dregs as a sole nitrogen source, a two-stage fermentation process is adopted for fermentation, finally the microorganism strains in a fermentation bacterial liquid are in the dormant state, and the final biologic deodorant is obtained; when the biologic deodorant is used, after water is added and the biologic deodorant is in contact with air, the microorganism strains can continuously grow and generate more enzymes. By adopting the biologic deodorant provided by the invention, organic matters which can generate odor can be fundamentally removed, the degree that a pipeline is blocked by organic matters is reduced or eliminated, and the biologic deodorant can be also used for washing oil-containing or/and protein-containing or/and starch-containing dirt, does not damage any pipeline in a sewer, and is environmental-friendly.

Description

technical field [0001] The invention belongs to the technical field of biological deodorization, and in particular relates to a biological deodorant and its preparation method and application. Background technique [0002] In the existing biological deodorants, product inhibition occurs in most of the liquid fermentation systems, which greatly reduces the amount of enzymes that degrade odors produced by bacteria in the liquid fermentation systems, making the production cycle shorter In addition, people often scald the sewer with boiling water to clear the sewer and deodorize the sewer, but boiling water will denature the protein or starch and tightly adhere to the pipe wall, making it more difficult to remove. Doing this for a long time will make the sewer worse. Blockage and the taste is more difficult to remove, and the bad smell or peculiar smell has not been eliminated at all. The current biological deodorant mainly decomposes odorous substances. When the biological deo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C02F3/34C11D3/386C12R1/72C12R1/10C12R1/125
Inventor 唐春红曾小清张忠民陈琳莉唐辉张乐延
Owner 重庆鸿紫圆光生物科技有限公司
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