15results about How to "Increase production intensity" patented technology

The invention discloses a breeding method of high-concentration cane molasses tolerant robust saccharomyces cerevisiae and high-concentration ethanol fermentation application of the high-concentration cane molasses tolerant robust saccharomyces cerevisiae, and belongs to the technical field of biology. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center on July 25, 2023, and the number of the Saccharomyces cerevisiae NGTM-F1 is GDMCC 63687. The Saccharomyces cerevisiae NGTM-F1 disclosed by the invention is named as Saccharomyces cerevisiae NGTM-F1. The saccharomyces cerevisiae is obtained by taking laboratory saccharomyces cerevisiae GJ08 as an original strain, carrying out ARTP (room temperature plasma) mutagenesis and adaptive evolution breeding and utilizing high-throughput screening, keeps a good growth state in cane molasses with the total sugar being 300 g / L, and has good fermentation performance in cane molasses with the total sugar being 250 g / L at the temperature of 30 DEG C. Under the condition of 30 DEG C in a conical flask, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 102.30 g / L, and the fermentation efficiency is 80.8%; under the condition of 30 DEG C in a 5 L fermentation tank, the total sugar consumed by fermentation is 250 g / L, the ethanol concentration can reach 111.65 g / L, and the fermentation efficiency is 87.0%.

The invention discloses corynebacterium glutamicum for producing N-acetylglucosamine and application of the corynebacterium glutamicum, and belongs to the technical field of synthetic biology. Specifically, the corynebacterium glutamicum overexpresses a GNA1 gene from a brewer's yeast S288C controlled by a tacM promoter, a glmS gene from corynebacterium glutamicum S9114 and a yqab gene from escherichia coli K12 MG1655, and simultaneously has five gene defect types of nagA, nagB, ldhA, msccg and msccg2 in a genome and two gene single base mutation types of odhA and glnA, and the codon of the glnA for coding the 405th amino acid is mutated from TAC to TTC. By blocking excretion of glutamic acid of corynebacterium glutamicum and enhancing conversion of glutamic acid to glutamine, the supply capacity of a precursor of corynebacterium glutamicum is enhanced, and an optimal way for synthesizing N-acetylglucosamine is obtained, so that efficient production of N-acetylglucosamine in corynebacterium glutamicum is realized, and the yield of N-acetylglucosamine in corynebacterium glutamicum is increased. The method has important economic value and social significance.

The utility model relates to the technical field of reactors, in particular to a reactor for synthesizing vinyl chloride and a system for synthesizing vinyl chloride. The reactor shell comprises a cylindrical barrel, an upper sealing head and a lower sealing head; an upper top plate is fixedly arranged between the cylinder body and the upper sealing head; a lower bottom plate is fixedly arranged between the cylinder body and the lower sealing head; a plurality of heat exchange tubes are fixedly connected between the upper top plate and the lower bottom plate; the heat exchange tubes are of a hollow structure, and a hollow core tube with the two ends closed is arranged in the center of each heat exchange tube. The core tube is movably connected in the heat exchange tube through a positioning sheet; and the core tube and the positioning sheet are fixedly and / or movably connected.

The invention provides a steam-gas mixed medium high-vacuum low-energy-consumption intelligent control system and a control method. Comprising a vapor phase precondenser, a vapor phase precondenser steam extraction opening pressure sensor, a vapor phase precondenser cooling water inlet control valve, a vapor phase precondenser cooling water inlet pressure sensor, a multi-channel efficient steam jet vacuum pump, a multi-channel efficient steam jet vacuum pump steam extraction opening pressure sensor and a multi-channel efficient steam jet vacuum pump injection steam inlet control valve. The system comprises a multi-channel efficient steam jet vacuum pump, a steam jet pressure sensor, a steam phase final cooler, a steam phase final cooler pressure sensor, a steam phase final cooler cooling water inlet control valve, a steam phase final cooler water inlet pressure sensor, a low-power-consumption auxiliary pump, a low-power-consumption auxiliary pump extraction opening control valve and a system controller. The system is designed according to the vacuum suction characteristic of a steam-gas mixed medium, the vacuum degree of the system can be remarkably improved, the energy consumption can be remarkably reduced, and therefore the heat energy utilization efficiency and the self-control capacity of a front-end system can be remarkably improved, the production efficiency is improved, the yield is increased, the efficiency is improved, and remarkable economic benefits are created.

The invention belongs to the field of organic matter synthesis, and particularly discloses a preparation method of tert-dodecyl mercaptan, which is characterized in that hydrogen sulfide and dodecene are used as raw materials, and an addition reaction is performed under the catalytic action of a solid acid catalyst by adopting a liquid-solid heterogeneous process and a fixed bed reactor to obtain the tert-dodecyl mercaptan. The continuous liquid-solid reaction is realized, the diffusion time of hydrogen sulfide to a liquid phase main body is shortened, the heat capacity and the heat transfer coefficient of a reaction system are improved, the catalytic efficiency of the catalyst and the temperature controllability of the reaction process are improved, and the reactor design is simplified. Under proper operation conditions, the conversion per pass of dodecene is greater than or equal to 85%, the selectivity is close to 100%, the technological process is simple, few three wastes are generated, treatment is easy, and the method is suitable for industrial production.

The invention belongs to the technical field of biology, and particularly relates to rhodococcus ruber and application thereof. The rhodococcus ruber AR-300 which can be used for efficiently preparing 2, 6-difluorobenzamide and other various amide substances is screened through ARTP mutagenesis and adaptive evolution, and the preservation number of the rhodococcus ruber AR-300 is CGMCC (China General Microbiological Culture Collection Center) No.31246. The rhodococcus ruber AR-300 can be used for efficiently preparing 2, 6-difluorobenzamide and other various amide substances. The nitrile hydratase activity of the strain can reach 5500 U.mL <-1 >, and the highest feeding concentration can reach 7 mol / L. When the Rhodococcus ruber CGMCC No.31246 provided by the invention is used for conversion, the effect of rapidly producing 2, 6-difluorobenzamide and other amide substances can be achieved, the purpose of improving the production efficiency is achieved, the environmental protection requirement is met, the production cost is reduced, and the method is suitable for industrial production. The method has a wide industrial application prospect.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

The culture medium comprises methionine, a carbon source, a nitrogen source and a phosphorus source, and the contents of the methionine, the carbon source, the nitrogen source and the phosphorus source in the culture process are 0.3-0.5 g / L, 180-230 g / L, 19-27 g / L and 3-5 g / L respectively. The optimized culture medium for improving the yield of the intracellular antibiotics can be used for fermenting penicillium brevicompactum to produce mycophenolic acid, and the titer of the penicillium brevicompactum for producing the mycophenolic acid can be obviously improved in a short time by utilizing the optimized culture medium and the fermentation method which are prepared by the invention; by utilizing the culture medium prepared by the invention, the activity of the mycophenolic acid produced by the mould in the penicillium brevicompactum can be improved.

The invention relates to the technical field of microorganisms, in particular to recombinant genetically engineered bacteria of yarrowia lipolytica as well as a construction method and application of the recombinant genetically engineered bacteria. The succinic acid production performance of the recombinant genetically engineered bacterium of yarrowia lipolytica is remarkably improved, high succinic acid yield and production intensity are achieved, the recombinant genetically engineered bacterium can be applied to stable and efficient production of succinic acid, the performance of the recombinant genetically engineered bacterium is stable, and the recombinant genetically engineered bacterium can be suitable for large-scale industrial production of succinic acid.

The invention discloses a cellobiose epimerase mutant and application thereof, and belongs to the technical field of bioengineering. The mutant is obtained by simultaneously carrying out site-directed mutagenesis on seven amino acids of an amino acid sequence as shown in SEQ ID NO.2; the site-specific mutagenesis is that the 17th arginine of the amino acid sequence shown as SEQ ID NO.2 is mutated into glutamine, the 24th alanine is mutated into serine, the 64th isoleucine is mutated into valine, the 184th leucine is mutated into serine, the 243th phenylalanine is mutated into leucine, the 339th glutamic acid is mutated into aspartic acid, and the 340th lysine is mutated into isoleucine. The mutant can be applied to the field of industrial lactulose production, lactulose can be efficiently prepared, the production intensity is high, the catalytic effect on a high-concentration substrate is good, enzyme shows better stability, efficient conversion of lactulose can be achieved, and a favorable basis is further provided for industrial application of cellobiose epimerase.

The invention discloses an expression cassette, a genetically engineered bacterium for producing ergothioneine and application of the genetically engineered bacterium. The expression cassette comprises coding genes of Ncegt1, egtEncr and egtDAfr, and the coding genes of the egtEncr and the egtDAfr are shown in the description. The amino acid sequences of Ncegt1, egtEncr and egtDAfr are respectively as shown in SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The expression cassette has the advantage of being capable of improving the fermentation yield of the ergothioneine, and the genetically engineered bacterium for producing the ergothioneine can autonomously produce histidine which is one of substrates, has the advantages of being high in fermentation yield, high in production intensity and low in cost when being applied to production, and has industrial application prospects.

The invention relates to the technical field of polyvinyl chloride, and discloses a polyvinyl chloride production process based on hot water feeding, which comprises the following steps: S1, raw material preparation: preparing raw materials meeting purity and quality requirements; s2, feeding water and monomers: injecting hot water at a preset temperature into a polymerization kettle, and then feeding the monomers; s3, adding a dispersing agent: adding the dispersing agent into the polymerization kettle; s4, coarse material prediction: observing and predicting the stability condition of a dispersion system in the kettle; s5, adding an initiator: adding the initiator into the polymerization kettle; s6, adding a terminating agent; s7, performing subsequent treatment; according to the invention, a hot water feeding processing technology is adopted, so that the heating period is eliminated, the purposes of shortening the PVC polymerization period and improving the production intensity of the polymerization kettle are achieved, meanwhile, heat balance is carried out on a polymerization system, and the temperature of the polymerization kettle is equal to the temperature of the polymerization reaction after the materials are added by controlling the addition amount of cold and hot deionized water.

The present invention provides a method using 136m 3 The method for producing SG-8 type polyvinyl chloride resin in a polymerization kettle belongs to the technical field of polyvinyl chloride resin production, comprising: using an anti-sticking kettle agent to treat 136m 3 The anti-sticking process of the polymerization kettle is to coat the wall; the feeding process is to add the auxiliary agent, water and vinyl chloride monomer to the polymerization kettle after the wall coating; the reaction process is to add the initiator to the polymerization kettle after the feeding to initiate the polymerization reaction; and the terminator is added to the polymerization kettle to terminate the reaction and the unloading process is to discharge the SG-8 type polyvinyl chloride resin slurry in the polymerization kettle. The present invention adopts 136m for the first time 3 The polymerization kettle produces SG-8 polyvinyl chloride resin, and the transformation is carried out on the existing SG-5 polyvinyl chloride resin production equipment, which effectively improves the utilization rate of the polymerization kettle, and also improves the production intensity and production efficiency of a single kettle, and the product quality is good, thereby increasing the production capacity of SG-8 polyvinyl chloride resin and reducing production energy consumption and costs.

A coal seam plasma gasification system and method, in which a conversion channel vertically passes through the overburden and the upper edge of the coal seam from the ground and then gradually deflects horizontally to form a non-vertical channel in the coal seam, and the non-vertical channel extends a certain distance in the coal seam, and the other side vertically passes through the overburden and the coal seam from the ground and is connected to the non-vertical channel; one end of the reaction tube is connected to the reaction tube driving device and the injection device on the ground, and the other end in the non-vertical channel is connected to the plasma generator, and the plasma generator generates high-temperature and high-activity particles through the reaction medium provided by the injection tube to convert the coal seam into coal gas; the collection and conversion channels are connected to collect coal gas, and a comprehensive testing device is arranged on the ground part of the collection pipe to test the composition, temperature and pressure of the collected coal gas, and a flow testing device is arranged on the ground part of the collection pipe to test the collected coal gas flow. This system realizes efficient and flexible in-situ gasification control of coal seams.

A system and method for producing methane by in-situ plasma conversion of coal seams, in which a plasma generator is movably arranged in a coal seam channel, and receives working medium and cooling medium from a working medium pipeline, and ionizes the working medium with high pressure to produce high-temperature and high-activity water vapor particles and high-temperature and high-activity hydrogen particles, and the high-temperature and high-activity water vapor particles undergo a reduction reaction with the coal seam to be converted into hydrogen and carbon monoxide, and the high-temperature and high-activity hydrogen particles undergo a methanogenic reaction in situ in a reaction chamber to produce methane, and the methane is output from the vertical well. The system simplifies the process of producing methane from coal gasification, has low production costs, and high conversion efficiency.