9results about How to "Increase production intensity" patented technology

The invention discloses corynebacterium glutamicum for producing N-acetylglucosamine and application of the corynebacterium glutamicum, and belongs to the technical field of synthetic biology. Specifically, the corynebacterium glutamicum overexpresses a GNA1 gene from a brewer's yeast S288C controlled by a tacM promoter, a glmS gene from corynebacterium glutamicum S9114 and a yqab gene from escherichia coli K12 MG1655, and simultaneously has five gene defect types of nagA, nagB, ldhA, msccg and msccg2 in a genome and two gene single base mutation types of odhA and glnA, and the codon of the glnA for coding the 405th amino acid is mutated from TAC to TTC. By blocking excretion of glutamic acid of corynebacterium glutamicum and enhancing conversion of glutamic acid to glutamine, the supply capacity of a precursor of corynebacterium glutamicum is enhanced, and an optimal way for synthesizing N-acetylglucosamine is obtained, so that efficient production of N-acetylglucosamine in corynebacterium glutamicum is realized, and the yield of N-acetylglucosamine in corynebacterium glutamicum is increased. The method has important economic value and social significance.

The invention belongs to the field of organic matter synthesis, and particularly discloses a preparation method of tert-dodecyl mercaptan, which is characterized in that hydrogen sulfide and dodecene are used as raw materials, and an addition reaction is performed under the catalytic action of a solid acid catalyst by adopting a liquid-solid heterogeneous process and a fixed bed reactor to obtain the tert-dodecyl mercaptan. The continuous liquid-solid reaction is realized, the diffusion time of hydrogen sulfide to a liquid phase main body is shortened, the heat capacity and the heat transfer coefficient of a reaction system are improved, the catalytic efficiency of the catalyst and the temperature controllability of the reaction process are improved, and the reactor design is simplified. Under proper operation conditions, the conversion per pass of dodecene is greater than or equal to 85%, the selectivity is close to 100%, the technological process is simple, few three wastes are generated, treatment is easy, and the method is suitable for industrial production.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

The invention relates to the technical field of microorganisms, in particular to recombinant genetically engineered bacteria of yarrowia lipolytica as well as a construction method and application of the recombinant genetically engineered bacteria. The succinic acid production performance of the recombinant genetically engineered bacterium of yarrowia lipolytica is remarkably improved, high succinic acid yield and production intensity are achieved, the recombinant genetically engineered bacterium can be applied to stable and efficient production of succinic acid, the performance of the recombinant genetically engineered bacterium is stable, and the recombinant genetically engineered bacterium can be suitable for large-scale industrial production of succinic acid.

The invention discloses an expression cassette, a genetically engineered bacterium for producing ergothioneine and application of the genetically engineered bacterium. The expression cassette comprises coding genes of Ncegt1, egtEncr and egtDAfr, and the coding genes of the egtEncr and the egtDAfr are shown in the description. The amino acid sequences of Ncegt1, egtEncr and egtDAfr are respectively as shown in SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The expression cassette has the advantage of being capable of improving the fermentation yield of the ergothioneine, and the genetically engineered bacterium for producing the ergothioneine can autonomously produce histidine which is one of substrates, has the advantages of being high in fermentation yield, high in production intensity and low in cost when being applied to production, and has industrial application prospects.

The invention relates to the technical field of polyvinyl chloride, and discloses a polyvinyl chloride production process based on hot water feeding, which comprises the following steps: S1, raw material preparation: preparing raw materials meeting purity and quality requirements; s2, feeding water and monomers: injecting hot water at a preset temperature into a polymerization kettle, and then feeding the monomers; s3, adding a dispersing agent: adding the dispersing agent into the polymerization kettle; s4, coarse material prediction: observing and predicting the stability condition of a dispersion system in the kettle; s5, adding an initiator: adding the initiator into the polymerization kettle; s6, adding a terminating agent; s7, performing subsequent treatment; according to the invention, a hot water feeding processing technology is adopted, so that the heating period is eliminated, the purposes of shortening the PVC polymerization period and improving the production intensity of the polymerization kettle are achieved, meanwhile, heat balance is carried out on a polymerization system, and the temperature of the polymerization kettle is equal to the temperature of the polymerization reaction after the materials are added by controlling the addition amount of cold and hot deionized water.

The present invention provides a method using 136m 3 The method for producing SG-8 type polyvinyl chloride resin in a polymerization kettle belongs to the technical field of polyvinyl chloride resin production, comprising: using an anti-sticking kettle agent to treat 136m 3 The anti-sticking process of the polymerization kettle is to coat the wall; the feeding process is to add the auxiliary agent, water and vinyl chloride monomer to the polymerization kettle after the wall coating; the reaction process is to add the initiator to the polymerization kettle after the feeding to initiate the polymerization reaction; and the terminator is added to the polymerization kettle to terminate the reaction and the unloading process is to discharge the SG-8 type polyvinyl chloride resin slurry in the polymerization kettle. The present invention adopts 136m for the first time 3 The polymerization kettle produces SG-8 polyvinyl chloride resin, and the transformation is carried out on the existing SG-5 polyvinyl chloride resin production equipment, which effectively improves the utilization rate of the polymerization kettle, and also improves the production intensity and production efficiency of a single kettle, and the product quality is good, thereby increasing the production capacity of SG-8 polyvinyl chloride resin and reducing production energy consumption and costs.

A system and method for producing methane by in-situ plasma conversion of coal seams, in which a plasma generator is movably arranged in a coal seam channel, and receives working medium and cooling medium from a working medium pipeline, and ionizes the working medium with high pressure to produce high-temperature and high-activity water vapor particles and high-temperature and high-activity hydrogen particles, and the high-temperature and high-activity water vapor particles undergo a reduction reaction with the coal seam to be converted into hydrogen and carbon monoxide, and the high-temperature and high-activity hydrogen particles undergo a methanogenic reaction in situ in a reaction chamber to produce methane, and the methane is output from the vertical well. The system simplifies the process of producing methane from coal gasification, has low production costs, and high conversion efficiency.