83results about How to "Improve growth traits" patented technology

The invention discloses a microbial fertilizer-plant synbiotic composition preparation and a method for preparing the synbiotic preparation. In the composition preparation, the plant synbiotic consists of probiotics, prebiotics and stabilizer; and bacillus forms zymotic fluid. The method for preparing the synbiotic preparation comprises the following steps: fermenting the probiotics; regulating bacteria amount; and preparing and homogenizing to obtain the product. Azotobacter, pralidoxime iodide and potassium bacteria in the preparation can increase soil nutrition, improve the fertilizer utilization rate and soil granule structures, promote the air permeability, water-retaining property and drainage, and overcome the defect of sunlight greenhouse continuous cropping; oligosaccharide prebiotics in the preparation can stimulate crops to improve resistance to pathogenic microorganisms, and promotes permanent planting and proliferation of the probiotics; the preparation can inhibit toxicity generated by bacteria, start self immunity system, eliminate diseases and remove evils, reduce bactericidal pesticide, has cold resistance, heat resistance, drought resistance, waterlogging resistance, saline and alkaline resistance, fertilizer damage resistance, chemical damage resistance and nutrition imbalance resistance, can promote plant root elongation and good development of capillary roots, and promote blooming and fruit yielding.

The invention provides a genetic engineering bacterium capable of producing uridine at high yield as well as a building method and application thereof. The genetic engineering bacterium is characterized in that pyrimidine nucleoside operons pyrBCAKDFE with the nucleotide sequence shown as SEQ ID NO:1 is integrated on a genome of colon bacillus; the starting is realized by a strong promoter P[trc];a uridine synthesis path is reconstituted; the self PRPP synthetase coding gene prsA on the genome is subjected to dual copying, and the starting is realized by the strong promoter P[trc]; meanwhile,the activity of udk, udp, and rihA, rihB and rihC is lacked; the thrA activity is lacked; the argF activity is lacked. The genetic engineering bacterium is applied to fermentation production of uridine; 40 to 67g / L of uridine can be produced after the fermentation is performed for 40 to 70h in a 5L fermentation tank; the maximum production intensity can reach 1.5g / (L*h); the glucoside conversionrate is 15 to 25 percent; the genetic engineering bacterium belongs to the highest level for producing the uridine by the fermentation method reported in the prior art.

The invention relates to a recyclable cutting substance for growing lithocarpus polystachyus seedlings and application of the recyclable cutting substance. The recyclable cutting substance is mainly prepared from the following materials in parts by weight: 5-6.5 parts of yellow soil, 0.5-1 part of perlite, 1-2 parts of peat soil and 1-2 parts of coco coir. The substances with different components are matched according to the growth characters of the lithocarpus polystachyus so as to prepare the recyclable cutting substance with excellent performance, and the survival rate of cut shoots is increased above 98%; and the bred cut shoots can be easily taken out, so that the root damaging risk is reduced, the later-period field planting is facilitated, after the cut shoots are planted outside, a cutting bed can be repeatedly used after being subjected to outplanting and surface leveling, and the recyclable cutting substance has a very high use value. The recyclable cutting substance is simple, feasible, efficient and cheap, the breeding cycle of nursery stocks is shortened, and the seedling growing cost is lowered; and the recyclable cutting substance can be directly applied to actual production and has a relatively high economic benefit.

The invention discloses a method for overcoming topophysis in vegetative propagation of gingkoes, which includes the following steps: taking a healthy and full-sprouted branch being without diseases and insect pests and growing at the middle upper part of a crown on the sunny side as a cutting slip; and adopting the cuttage and stumping method to solve the problem of topophysis during the vegetative propagation of gingkoes. Multiple branches generated after stumping can be directly adopted for cuttage or propagation by grafting, and the main branch can grow vertically. According to the method, the cost is low, the seedling forming speed is high, the rate of survival is high; and the problem of quick breeding of good seed sources, pedigree, clone and excellent single plant of gingkoes can be solved.

The invention discloses a huso dauricus female fish and amur sturgeon male fish hybridization fish breeding method and belongs to the technical field of aquaculture. The method includes the steps that firstly, parent selection is conducted, wherein a huso dauricus at age 14+ is selected as a female parent, and an amur sturgeon at age 8+ is selected as a male parent; secondly, parent fish breeding and reproductive regulation are conducted, wherein S1, the parent fishes are selected, S2, feeds of the parent fishes are selected, the parent fishes are fed and nutrient enrichment is performed, S3, water temperature and reproductive regulation is performed, and S4, breeding condition management and daily management are performed; thirdly, artificial fertilization and incubation are conducted, S1, egg stripping and semen collection are performed, S2, artificial fertilization is performed, S3, egg washing is performed, and S4, fertilized ovum incubation is performed; fourthly, larval fish and juvenile fish breeding is conducted. The hybridized sturgeon fry bred through the method are high in fertilization rate and incubation rate, and have the advantages of being excellent in growth trait, and specifically speaking, the hybridized sturgeon fry are quick in growing, wide in adaptability, high in stress resistance and the like, and therefore the method is suitable for breeding of sturgeons in different regions of China.

The invention discloses an RFLP method for rapidly detecting the SNP of the cattle FGF21 gene. Using the whole genome DNA of the cattle to be tested containing the FGF21 gene as a template, using primer pairs P1, P2 and P3 as primers, the FGF21 gene of cattle is amplified by PCR, and 4 SNP sites; primer pairs F159, F297, F940, and F1151 were designed for these 4 SNP sites, and SalI, XhoI, XbaI, and MspI restriction sites were artificially introduced in turn for PCR amplification. The endonucleases digested the PCR amplification products respectively, and then detected the digested fragments by agarose gel electrophoresis, and identified the 159th, 297th, 940th, and 1151st units of the cattle FGF21 gene according to the electrophoresis results. Nucleotide polymorphism. Since the function of the FGF21 gene involves growth traits such as body weight, the detection method provided by the invention lays the foundation for the establishment of the relationship between the SNP of the FGF21 gene and the growth traits, so as to be used for marker-assisted selection of the growth traits of Chinese cattle, and to quickly establish cattle with excellent genetic resources. population.

The invention relates to a method for producing pleurotus cornucopiae by taking slag powder as a cultivation material. A raw material comprises the components in parts by weight as follows: 5-20 parts of slag powder, 70-85 parts of a substrate raw material, 0.3-0.5 part of urea, 2-4 parts of a compound fertilizer, 2-5 parts of lime and 1-2 parts of plant ash. In the raw material of a culture substrate, maize straw or cottonseed hulls is (are) replaced with the slag powder, so that the cost of the culture substrate is reduced obviously. According to the method for producing the pleurotus cornucopiae, the operation is simple, the output of the pleurotus cornucopiae is increased greatly, the problem that the slag power pollutes the environment is solved, waste is turned into wealth, and the slag power is used, taken as a fertilizer and then returned to the field.

The invention discloses a fast detection method for chicken Pax7 gene 31 bp indel polymorphism and the application thereof, wherein the detection method comprises the following steps: designing a pair of primers based on gene sequences of a third exon and a third intron of a chicken Pax7 gene; then performing PCR amplification; and finally detecting the 31 bp indel polymorphism of the gene through agarose gel electrophoresis, when the third intron 31 bp is inserted, the gene is an II genotype, when the third intron 31 bp is in deletion, the gene is a DD genotype, and when the locus is a heterozygous individual, the gene is an ID genotype. Meanwhile, the method utilizes detection result to perform relevance analyze with chicken economical characters, which is used for chicken assistant selection and molecular breeding. According to the invention, during big DNA fragment detection, the method not only has high resolution, sensitive detection and accurate type judgment, but also has the characteristics of simplicity in operation, time conservation and low cost, and can be widely popularized.

The invention discloses a method for establishing a giant grouper sperm freezer and assisting grouper distant hybridization breeding. The sperm freezer establishing method comprises the steps of preparing sperm diluent, preparing sperm cryopreservation liquid, freezing and storing sperms and establishing the sperm freezer. The grouper distant hybridization breeding method comprises the steps of establishing the giant grouper sperm freezer, establishing saladfish breeding groups, cultivating saladfish parent fish and performing reproductive regulation, performing artificially spawning breeding, fertilizing the frozen sperms and performing incubation, and cultivating fries. According to the method, the first giant grouper sperm freezer is established in China, reproductive isolation of the groupers caused by factors, such as geographical distribution, optimum temperature range and breeding time, is overcome, and distant hybridization breeding of the grouper groups is possible, so that distant hybridization breeding varieties are cultivated.

The invention relates to a hybrid breeding method for a female Siberian sturgeon and a male Amur sturgeon and belongs to the technical field of aquaculture. The method includes the following steps that 1, choice of parents is conducted, wherein a 5+ aged Siberian sturgeon is selected as the female parent, and a 7+ aged Amur sturgeon is selected as the male parent; 2, parent fish breeding and procreation regulation are conducted, wherein parent fish optimization, parent fish feed selection, feeding and nutrient enrichment, water temperature procreation regulation and breeding condition and daily management are conducted; 3, artificial fertilization and hatching are conducted, wherein spawn squeezing and semen collection, artificial fertilization, spawn washing and hatching of fertilized spawns are conducted; 4, breeding of fish larvae and young fishes is conducted. The hybrid sturgeon fry bred through the method are high in fertility rate and hatching rate and have the advantages of being high in growth speed, wide in adaptability, high in stress resistance, good in growth trait and the like, and the method is suitable for breeding sturgeons in different regions of China.

The invention discloses a hybridization fry breeding method of acipenser schrenckii female fish and polyodon spathula male fish. By using sex hormone and temperature water flow adjustment, spawning and spermiation of two kinds of sturgeon are synchronously conducted, the problem of difficult hybridization due to far genetic relationship of two kinds of sturgeon and non-overlapping natural breedingtime is solved, meanwhile, a mode that tubificidae opening and sturgeon feed domestication are combined is adopted, and a novel low-carbon sturgeon variety which is high in yield, good in quality, excellent in comprehensive performance and suitable for south high-temperature weather is bred. According to the hybridization fry breeding method, the fertilization rate of bred hybrid fry reaches up to 60%, the hatching rate reaches 7.5%, the tolerable temperature reaches 30 DEG C, and the bred hybrid fry have the advantages of high temperature resistance, wide application range, high stress resistance and the like and are suitable for breeding of Chinese South.

The invention discloses a molecular breeding method based on the single nucleotide polymorphism of cattle Angpt18 genes.The molecular breeding method includes the steps that to-be-detected cattle whole genome DNA with the Angpt18 genes serves as a template, primer pairs P serve as primers, the cattle Angpt18 genes are subjected to PCR amplification, and PCR amplification products are obtained; the PCR amplification products are digested with restriction enzyme HinfI to obtain enzyme-digested product fragments, and the enzyme-digested product fragments are subjected to polyacrylamide gel electrophoresis; the polyacrylamide-gel-electrophoresis result shows that the 308th bits of the cattle Angpt18 genes have the single nucleotide polymorphism; meanwhile, the detecting result of the single nucleotide polymorphism of the cattle Angpt18 genes and the growth characteristics of a cattle are analyzed in a relevance mode, suitable genotypes are used for improving molecular breeding of the growth characteristics of the cattle, and cattle populations with the excellent genetic resources can be rapidly established.

The invention discloses a method for detecting polymorphism of multiple single nucleotides in a 5' control region of a cattle CIDEC gene. The method comprises the following steps of: with a to-be-detected cattle complete genome DAN containing a CIDEC gene as a template and primer pairs P (P1, P2 and P3) as primers, performing amplification on the cattle CIDEC gene; respectively digesting PCR amplification products with restriction enzymes such as HaeIII, AccI and HaeIII, then carrying out agarose gel electrophoresis on a digested amplified fragment, and identifying polymorphism of single nucleotides at a 974th site, a 956th site and a 501st site of the CIDEC gene of the cattle according to results of agarose gel electrophoresis, wherein polymorphism of the single nucleotide at the 956th site is also the polymorphism of single nucleotides at a 841st site, a 763rd site, 727th site and a 546th site, the polymorphism of the single nucleotide at the 501st site is also the polymorphism of a single nucleotide at a 643rd site owning to complete linkage, and thus polymorphism information of nine sites can be obtained by detecting polymorphism of three sites. The detection method provided by the invention lays a foundation for establishing a relation between the polymorphism of single nucleotides of the CIDEC gene and growth traits, so as to be convenient for marker assisted selection (MAS) on the growth traits for cattle beef in China, and a cattle population with excellent genetic resources can be quickly established.

The invention relates to a related gene for high-yield L-leucine, a construction method of engineering bacteria and application. The construction method for the engineering bacteria comprises the following steps: (1) integrating a leuA mutant gene to a cgl1135 pseudogene locus, wherein the leuA mutant gene is as shown in sequence 1; (2) integrating an ilvBN mutant gene to a cgl1890 pseudogene locus, wherein the ilvBN mutant gene is as shown in sequence 2; and (3) replacing a citrate synthase primary promoter by a PCP_2928 specific promoter, wherein the PCP_2928 specific promoter is as shown insequence 3. By adopting the technical scheme provided by the invention, 32 g / L L-leucine can be produced by fermenting for 30 hours in a shaking flask, and 60 g / L L-leucine can be produced by fermenting for about 50 hours in a 5L fermenting tank; the maximum production intensity can reach 1.5 g / (L*h); the saccharic acid conversion rate is 30 percent; and the production level is the highest levelfor producing the L-leucine by the fermenting method reported at present.

The present invention discloses single nucleotide polymorphism, a detection method and an application of chicken miRNA-1704 gene, wherein the single nucleotide polymorphism is that the site 148 in the chicken miRNA-1704 gene sequence represented by SEQ ID NO.3 is C or G, and the detection method comprises: adopting DNA of the chicken complete-genome containing the miRNA-1704 gene as a template, designing a pair of primers, amplifying, carrying out digestion on the PCR amplification product, and identifying whether the C and G single nucleotide polymorphism exists on the site 148 in the chicken miRNA-1704 gene. In addition, the detection result and the chicken growth traits are adopted to carry out correlation analysis, such that the results show that the site is significantly associated with the weights at different developmental stages; and the detection method can be used for chicken assisted selection and molecular breeding so as to rapidly establish chicken flocks with excellent genetic resources.

The invention provides a detection method of single nucleotide polymorphism and molecular markers of cattle FBXO32 genes. Cattle full genome deoxyribonucleic acid (DNA) to be detected comprises FBXO32 genes and serves as a template. A primer pair P (P1, P2 and P3) serves as a primer. Cattle FBXO32 genes are amplified through polymerase chain reaction (PCR). PCR amplification products are digested by restriction endonuclease HaeIII, HaeIII and BfaI respectively, agarose gel electrophoresis is performed on amplification segments after enzyme digestion, and single nucleotide polymorphism at 22830 position, 44675 position and 53423 position of the cattle FBXO32 genes (national center of biotechnology information (NCBI) reference serial number: NC:_007312.4) is identified according to agarose gel electrophoresis results. The detection method lays a base for establishment of the relationship between the single nucleotide polymorphism and growth trait of the FBXO32 genes, can be used for marker assisted selection (MAS) of the growth trait for Chinese cattle conveniently, and establishes cattle population with good genetic resources rapidly.

The invention discloses a molecular marker for identifying duck carcass performance in the early stage and an identification method and application thereof. The molecular marker is a polymorphic sitedeveloped on the basis of insulin-like growth factors and protein gene IGF2BP1 and is capable of affecting the expression quantity of the protein gene IGF2BP1, the molecular marker is an insertion / deletion sequence located at the 1386th-1408th site of the IGF2BP1 gene sequence, the length of the insertion / deletion sequence is 23bp, and the nucleotide sequence of the insertion / deletion sequence isas shown in SEQ ID No. 2; by identifying the types of the molecular marker in a duck genome, the duck carcass performance can be judged through molecular screening in the early stage, and a direct technological means is provided for local small-scale sheldrake breeding; by early-stage breeding, feeding cost can be lowered while growth traits can be genetically fundamentally improved, and accordingly genetic breeding progress can be accelerated.

The invention provides a genetically engineered bacterium for producing 5-hydroxytryptophan, and a construction method and application of the genetically engineered bacterium. Firstly, a tryptophan synthesis pathway in escherichia coli and a metabolic flux related to tryptophan in a whole amino acid metabolic network are analyzed and reconstructed, and supply of 5-HTP precursor tryptophan is enhanced; and then a human-derived type-2 tryptophan hydroxylase mutant and a tetrahydropterin synthesis and regeneration approach are introduced, a tryptophan intracellular hydroxylation reaction is broken through, the plasmid-free genetically engineered bacterium which has a clear genetic background and is capable of efficiently and stably producing 5-HTP through a microbial direct fermentation method is obtained, and the plasmid-free genetically engineered bacterium has a good application value.

The invention discloses a method for quickly detecting RFLP (restriction fragment length polymorphism) of SNP (single nucleotide polymorphism) of a common ox SIRT1 (silent information regulator 1) gene promotor region. The method comprises the following steps of: taking whole genome DNA (deoxyribonucleic acid) of common ox blood as a template and a primer pair P (F and R) as a primer; performing PCR (polymerase chain reaction) amplification on the common ox SIRT1 gene promotor region; digesting a PCR amplification product by using restriction endonuclease SmaI; performing agarose gel electrophoresis on segments after enzyme digestion; and identifying the SNP of the common ox SIRT1 promotor region according to a result of the agarose gel electrophoresis. Because important functions including muscle differentiation, lipogenesis and the like related to the SIRT1 are closely related to common ox meat quality characteristics, and the SNP can significantly affect the promotor activity of the SIRT1 gene and is related to various important economic characteristics of the common ox, the detection method provided by the invention lays a foundation for the establishment of the relationship between the SNP and growth characteristics of the SIRT1 gene, and can be used for marker assisted selection of the growth characteristics of the Chinese common ox so as to facilitate rapid establishment of common ox populations with excellent genetic resources.

The invention discloses a method and special kit for rapidly detecting single nucleotide polymorphism of a cattle CRABP2 gene. The method comprises the following steps: carrying out PCR amplification on the cattle CRABP2 gene by taking cattle whole genome DNA as a template and primer pairs P1 and P2 as primers; after a PCR product is digested by virtue of restriction endonucleases ApaI and Sal I, respectively, carrying out agarose gel electrophoresis; and identifying the single nucleotide polymorphism of a site 2458 and a site 2878 of the cattle CRABP2 gene. According to the method, the single nucleotide polymorphism of the cattle CRABP2 gene can be detected, and relevant molecular genetic markers close to the growth character of the cattle are screened and detected from a DNA level and are applied to the assistant selection and molecular breeding of the cattle, so that the stock breeding speed of the local cattle is increased.

The invention relates to a complex breeding method for improving a growth trait of Pelteobagrus fulvidraco. The complex breeding method includes the following steps: acquiring high-quality Pelteobagrus fulvidraco and Pelteobagrus vachelli parent library through second generation group breeding; using microsatellite markers to detect Pelteobagrus fulvidraco and Pelteobagrus vachelli parents, selecting parent individuals having the genetic similarity of above 90% as a reproduction group; using female Pelteobagrus fulvidraco as female parents and male Pelteobagrus vachelli as male parents to perform hybrid breeding; and acquiring hybrid Pelteobagrus fulvidraco having the growth speed above 20% faster than common Pelteobagrus fulvidraco, wherein the hybrid Pelteobagrus fulvidraco has both excellent traits of dual parents, an attracting color ( female parents) and fast growth speed ( male parents). The complex breeding method can acquire improved hybrid Pelteobagrus fulvidraco, and has important significance for increasing the unit yield, the breeding benefits, and serving production practice.

The invention discloses a special formula feed for wool rabbits. The formula feed contains the following components in parts by weight: 25-30 parts of corn, 15-20 parts of wheat, 8-10 parts of bean cakes, 5-8 parts of rapeseed cakes, 10-15 parts of feed yeast, 10-14 parts of pine needle powder, 10-14 parts of folium artemisiae argyi powder, 0.5-1.5 parts of zeolite powder, 0.01-0.02 part of lysine, 0.45-0.8 part of sulfur-containing amino acid, 0.03-0.05 part of rare earth elements, 0.51-0.63 part of compound vitamin, 0.5-1.0 part of compound microelement additive and 0.5-1.0 part of compound Chinese herbal medicine additive. The invention also discloses a preparation method of the special formula feed for wool rabbits. The composition is rich in nutrients, can satisfy the nutritional level required by wool rabbit production, has the effect of promoting improvement of the wool yielding properties of the wool rabbits and does not use animal derived feed raw materials, thus not only reducing the feed cost, but also meeting the requirements of herbivores for feed sources.

The invention discloses a construction method for a breeding pedigree of lutraria sieboldii. The lutraria sieboldii is a rare bivalve shellfish, and excessive breeding causes problems such as germplasm degeneration and frequent diseases; and pedigree breeding becomes a first choice for industrial development, but because of a subtidal open water area breeding environment of the lutraria sieboldii,complex resources, and a large number of individuals to be marked, common marking and molecular marking are difficult, and an effective pedigree breeding mechanism is not established in China. The method disclosed by the invention adopts the technical steps of artificial directional insemination, breeding, pedigree individual marking and cultivation to solve the difficult problem of pedigree construction of pedigree breeding of the lutraria sieboldii, can quickly realize the pedigree breeding by combining genetic polymorphism analysis, and provides technical references for pedigree breeding of the lutraria sieboldii and related subtidal bivalve shellfish.

The invention relates to an environment-friendly feed additive capable of reducing piglet weaning stress syndrome as well as a preparation method and application thereof, and belongs to the field of feed. The environment-friendly feed additive comprises the following raw materials: fermented rhizoma atractylodis sinensis, rhizoma atractylodis macrocephalae, pulicaria dysenterica oil, astragalus membranaceus, sorghum skin, Chinese fevervine herb and schisandra chinensis. The feed addtive is a traditional Chinese herbal medicine feed additive, and can enhance the immunological function of the organism and improve the quality of animal products; furthermore, the feed additive does not have residues, disease resistance and toxic or side effect, and can serve as an effective substitute of antibiotic and hormone feed additives. The preparation method of the environment-friendly feed additive comprsies the following steps: mixing the rhizoma atractylodis macrocephalae,the astragalus membranaceus, the sorghum skin, the Chinese fevervine herb and the schisandra chinensis according to the proportion to obtain a first mixture, mixing the first mixutre and the fermented rhizoma atractylodis sinensis, and then mixing the mixture and the pulicaria dysenterica oil. The method is simple, high in condition controllability and suitable for industrialized production. When the environment-friendlyfeed addtive is applied to preparation of pig feed, the growth of the weaned piglets can be promoted and the immunity of the piglets can be improved.

The invention relates to the technical field of feed, in particular to a piglet feed. The piglet feed is prepared from, low protein whey mist, bone meal, wheat middling, cicada chrysalis powder, yeast powder, bentonite, poria cocos, ageratum, radix curcumae, myrobalan, radish seed, cortex pseudolaricis, capillary artemisia, senna, radix achyranthis bidentatae, Chinese yam, radix aucklandiae, black fungus, monkey mushroom, root of chinese barberry, caulis spatholobi, enzymic preparations, probiotics, acidification agent, and emulsifying agent. According to the physiological property of the piglet, a reasonable formula is researched, and a safe and toxic-free feed with balance nutrition is provided for the piglet; the feed is rich in massive protein, fatty and probiotics; the feed has the advantages of effectively promoting the piglet's digestive absorption to the feed and guaranteeing the healthy growth of the piglet; meanwhile, the feed can effectively promote the development of piglet gastrointestinal function, increasing the probiotics gathering in the intestinal tract, effectively improving the disease resistance and increasing the survival rate of the piglet.

The invention discloses a molecular mark for affecting early-stage growth of sheep as well as a detection method and application of the molecular mark. The molecular marker for affecting the early-stage body weight of the sheep is located in a sheep PLAG1 gene NC 040260.1:g.39071254 locus, and has C / T polymorphism, and the birth weight and weaning weight of the CT type sheep are significantly higher than those of CC type sheep. The method for screening early-stage fast-growth sheep breeds (lines) includes the steps of detecting the C / T polymorphism of the sheep PLAG1 gene NC 040260.1:g.39071254 locus, and selecting CT type individuals for seed retention. The polymorphic locus can be used for early-stage auxiliary selection of sheep growth traits, improve the selection accuracy, and speed up the breeding process.

The invention provides a cultivation method for rapidly growing triploid crassostrea gigas Haida No.1. The method comprises the following steps: obtaining high-heterozygosity triploid crassostrea gigas Haida No.1 by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas Haida No.1, taking females of the triploid crassostrea gigas Haida No.1 as female parents, taking males of the diploid Haida No.1 as male parents, and obtaining tetraploid crassostrea gigas Haida No.1 with improved growth characters by inhibiting discharge of the first polar bodies of the fertilized eggs; and hybridizing males of the tetraploid crassostrea gigas Haida No.1 and the diploid crassostrea gigas Haida No.1 to obtain the triploid crassostrea gigas Haida No.1 which grows quickly. According to the method, the growth performance of the crassostrea gigas is improved by adopting a method for improving the heterozygosity of parent genes, and the tetraploid crassostrea gigas Haida No.1 obtained by adopting the method is hybridized with the diploid crassostrea gigas Haida No.1 which originally has the characteristic of rapid growth to obtain the triploid crassostrea gigas Haida No.1 with rapid growth performance.

The invention relates to an artificial breeding method of southern China beluga sturgeon. The method comprises the steps of 1, parent fish selection and winterization breeding; 2, parturition hastening and artificial insemination; 3, incubation management seedling emergency; 4, seedling breeding. The artificial breeding method has the advantages that the bred beluga sturgeon seedlings are high in fertilization rate and hatching rate (the insemination rate reaches 95% or above and the hatching rate reaches 90% or above) and have the advantages of being rapid in growth, large in adaptability, high in survival rate, high in stress resistance and good in other growth trait, has the higher economic benefits and is suitable for breeding of southern China beluga sturgeon.

The invention discloses a huso dauricus female fish and amur sturgeon male fish hybridization fish breeding method and belongs to the technical field of aquaculture. The method includes the steps that firstly, parent selection is conducted, wherein a huso dauricus at age 14+ is selected as a female parent, and an amur sturgeon at age 8+ is selected as a male parent; secondly, parent fish breeding and reproductive regulation are conducted, wherein S1, the parent fishes are selected, S2, feeds of the parent fishes are selected, the parent fishes are fed and nutrient enrichment is performed, S3, water temperature and reproductive regulation is performed, and S4, breeding condition management and daily management are performed; thirdly, artificial fertilization and incubation are conducted, S1, egg stripping and semen collection are performed, S2, artificial fertilization is performed, S3, egg washing is performed, and S4, fertilized ovum incubation is performed; fourthly, larval fish and juvenile fish breeding is conducted. The hybridized sturgeon fry bred through the method are high in fertilization rate and incubation rate, and have the advantages of being excellent in growth trait, and specifically speaking, the hybridized sturgeon fry are quick in growing, wide in adaptability, high in stress resistance and the like, and therefore the method is suitable for breeding of sturgeons in different regions of China.

The present invention discloses single nucleotide polymorphism, a detection method and an application of chicken miRNA-1704 gene, wherein the single nucleotide polymorphism is that the site 148 in the chicken miRNA-1704 gene sequence represented by SEQ ID NO.3 is C or G, and the detection method comprises: adopting DNA of the chicken complete-genome containing the miRNA-1704 gene as a template, designing a pair of primers, amplifying, carrying out digestion on the PCR amplification product, and identifying whether the C and G single nucleotide polymorphism exists on the site 148 in the chicken miRNA-1704 gene. In addition, the detection result and the chicken growth traits are adopted to carry out correlation analysis, such that the results show that the site is significantly associated with the weights at different developmental stages; and the detection method can be used for chicken assisted selection and molecular breeding so as to rapidly establish chicken flocks with excellent genetic resources.