100results about How to "Increase reproductive rate" patented technology

The invention discloses a sewage purifier, which comprises polyhydroxyalkanoate, diatomite, humic acid, a bioactivator, inorganic metal salt and other substances. The invention also discloses a sewage purification method by utilizing the sewage purifier. A completely biodegradable material polyhydroxyalkanoate is used as a sustained material of the bioactivator, and the completely biodegradable material is slowly degraded and releases the bioactivator so as to realize the long-term high-efficiency purification function of the bioactivator; and by combining the effects of aquatic plants, phosphorus, nitrogen, polycyclic aromatic hydrocarbon and heavy metals in the sewage are purified and treated, so the sewage purifier has better sewage treatment effect.

The invention provides a geobacillus sp. strain UTM02. The storage number of the geobacillus sp. strain UTM02 is CGMCC No.5642. The geobacillus sp. strain UTM02 can rapidly multiply at the temperature of 70 DEG C. The invention further provides solid compost inoculant prepared from the geobacillus sp. strain UTM02, and a preparation method and application of the solid compost inoculant. The compost inoculant is low in production cost, stable in quality and convenient to operation, can be applied to high-temperature sludge composting, does not affect moisture content of compost bodies, is environment-friendly and pollution-free, has excellent performances, achieves double effects of thermal energy utilization and waste treatment, and has good economic and social benefits.

The invention discloses a tissue culture rapid propagation method of Abelmoschus manihot, a plant of Abelmoschus in Malvaceae. The method comprises the three steps of obtaining sterile sprouts from seeds, multiplying the sprouts and inducing the sprouts to root so as to obtain a large quantity of Abelmoschus manihot seedlings in short time, wherein the step of multiplying sprouts is described as follows: cutting the stem of each sterile sprout in the seedling age of 25-50d into 1-2 cm stem sections with knots, implanting the stem sections into a culture medium of MS+6-BA1.0-1.5mg / L+NAA0.1-0.2mg / L, and culturing the stem sections under certain condition of temperature and sun lighting. The step of inducing sprouts to root is described as follows: cutting 1.0-2.0cm auxiliary buds obtained in the step of multiplying sprouts, implanting the auxiliary buds into a culture medium of 1 / 2MS+IBA0.4-0.6mg / L, and inducing the auxiliary buds to root under certain temperature and sun lighting. The invention has the advantages of simple method, high multiplying speed and stability, short culturing cycle and high yield. With higher feasibility in actual production and application, the invention is suitable for the industrialization production of Abelmoschus manihot.

The invention relates to artificial feed for myzus persicae, which comprises 2.5%-3.5% of amino acid mixture, 0.2%-0.3% of ascorbic trace element chelate, 0.1%-0.2% of vitamin B, 17%-20% of cane sugar, 3.0%-4.0% of tobacco juice, 0.4%-0.6% of KH2PO4, 0.1%-0.3% of MgCl2, 6H2O and 72%-75% of redistilled water. The artificial feed with the added tobacco juice has the evident effect on improving survival rate and reproductive rate of myzus persicae feeding the artificial feed. Breeding of myzus persicae with the artificial feed is insusceptible to seasons and available all the year round; mass breeding of myzus persicae is insusceptible to hosts and space; the impact of factors such as microbial infection, pesticides and the like can be relieved or avoided; and standard and uniform experimental myzus persicae is easy to obtain.

The invention discloses a tissue culture efficient breeding and seedling growing method of hybrid orchid Huangjinxiaoshentong. The tender shoot of the hybrid orchid Huangjinxiaoshentong is adopted as an explant and undergoes tissue culture to induce rhizome, and the rhizome is cultured only through using a medium in order to simultaneously complete propagation of the rhizome and the differentiation and growth of sprouts. The method has the advantages of large quantity, fast seedling emergence, high breeding rate and strong stability, obtained plants have the characteristics of strong sprouts, high quality and strong resistance, and the method can realize successful development of the factory production of the rapid breeding of hybrid orchid seedlings, and provides a method for the tissue culture of other orchid-like plants.

The invention discloses a method for overcoming topophysis in vegetative propagation of gingkoes, which includes the following steps: taking a healthy and full-sprouted branch being without diseases and insect pests and growing at the middle upper part of a crown on the sunny side as a cutting slip; and adopting the cuttage and stumping method to solve the problem of topophysis during the vegetative propagation of gingkoes. Multiple branches generated after stumping can be directly adopted for cuttage or propagation by grafting, and the main branch can grow vertically. According to the method, the cost is low, the seedling forming speed is high, the rate of survival is high; and the problem of quick breeding of good seed sources, pedigree, clone and excellent single plant of gingkoes can be solved.

The invention discloses a method for tissue culture and rapid propagation of rosaceae Rosa deciduous erect shrub Rosa rugosa Thunb. belonging to the technical field of horticultural plant seedling propagation. The method is used for carrying out isolated culture on edible Rosa rugosa Thunb. caulicles to build a tissue culture and rapid propagation system of Rosa rugosa Thunb. and comprises four stages, namely selection and sterilization, primary culture, multiplication culture and rooting culture of an explant. The method disclosed by the invention is simple and has the advantages of high reproduction rate, short culture period and high reproduction coefficient, has relatively strong practicability in the actual production application, solves the technical problems of low reproduction rate and long reproduction period of a Rosa rugosa Thunb. seedling, and is suitable for commercial production of Rosa rugosa Thunb. seedlings so as to meet the urgent demand of a market.

The invention discloses a method for quickly propagating plukenetia volubilis linneo. The method includes steps of disinfecting the surfaces of explants which are current-year non-lignified branches of the plukenetia volubilis linneo; cultivating and inducing callus tissues of the explants; inducing differentiation and elongation of buds by the aid of the callus tissues; strengthening and rooting seedlings; exercising and transplanting the seedlings and the like to obtain regenerated plants. The method has the advantages that integral procedures can be carried out under controllable conditions without seasonal constraints, the propagation cycle can be shortened to a great extent, the propagation multiples can be increased, the method has important practical significance on meeting requirements on high-quality seedlings of the plukenetia volubilis linneo and accelerating popularization of improved varieties of the plukenetia volubilis linneo, and an efficient and feasible technical means can be provided for future research on genetic modification and the like on the plukenetia volubilis linneo.

The invention discloses a human umbilical cord blood mesenchymal stem cell extract and a preparation method and application thereof. The human umbilical cord blood mesenchymal stem cell extract is prepared in the steps that 1, human umbilical cord blood mesenchymal stem cells are separated from human umbilical cord blood and cultured in a culture medium added with vitamin C; 2, the secreted content of the human umbilical cord blood mesenchymal stem cells is obtained from the culture medium where the human umbilical cord blood mesenchymal stem cells are cultured; 3, the cultured human umbilicalcord blood mesenchymal stem cells are crushed to obtain the non-secreted content of the human umbilical cord blood mesenchymal stem cells is obtained; 4, the secreted content and the non-secreted content are mixed, and the human umbilical cord blood mesenchymal stem cells separated from the human umbilical cord blood are fully used for preparing the extract containing the secreted content and thenon-secreted content. Besides, the invention further discloses a preparation method of the human umbilical cord blood mesenchymal stem cell extract and application of the human umbilical cord blood mesenchymal stem cell extract in cosmetics.

The invention involves a hybrid goose cultivation method which is used to producing goose fatty liver, it includes the following steps : (1) producing NanTu geese first hybrid F1 which lay more eggs, fertility rate and corrupt rate are high, fatty liver is heavier and quality is improved using the loosen and cultured NanXi White goose as female parent and the Toulouse geese as male parent; (2) cross-breeding the NanTu geese first hybrid F1 as female parent and the Lande Ash geese as male parent to producing the Nantu Lande geese second hybrid F2;(3) breeding the Nantu Lande geese second hybrid F2 to feeding weight, then filling fodder to producing fatty liver. The invention can improve breeding rate of hybrid geese, and performance of its production geese fatty liver is fine, enhance the adaptability and decrease elimination and mortality rate greatly.

The invention discloses a sewage purifier, which comprises polyhydroxyalkanoate, diatomite, humic acid, a bioactivator, inorganic metal salt and other substances. The invention also discloses a sewage purification method by utilizing the sewage purifier. A completely biodegradable material polyhydroxyalkanoate is used as a sustained material of the bioactivator, and the completely biodegradable material is slowly degraded and releases the bioactivator so as to realize the long-term high-efficiency purification function of the bioactivator; and by combining the effects of aquatic plants, phosphorus, nitrogen, polycyclic aromatic hydrocarbon and heavy metals in the sewage are purified and treated, so the sewage purifier has better sewage treatment effect.

The invention provides compositions and methods for preventing and treating osteochondrosis, lameness, and leg weakness by administration of supplemental boron and vitamin C containing compounds to animals and humans. The supplemental boron and vitamin C containing compounds are provided in animal feed compositions or as supplements for animal feed. Also provided by this invention are animal feed compositions that are supplemented with boron and vitamin C containing compounds and which have reduced phosphorus content. The invention also provides a method for decreasing the amount of phosphorus excreted by an animal, a method of increasing the efficiency of absorption of phosphorus by an animal, a method of reducing environmental phosphorus pollution by administering supplemental boron and vitamin C to the animal. The invention also provides a method of reducing pre-weaning mortality in an animal by feeding pregnant, nursing or lactating animals by administering supplemental boron and vitamin C containing compounds.

The invention discloses preparation and application of continuous cropping peanut resistant complex bacteria. The complex bacteria comprise continuous cropping peanut resistant bio-control bacteria and an allelochemicals degrading bacteria agent. By using the complex bacteria, the degradation efficiency of the continuous cropping peanut allelochemicals is high, the disease of continuous cropping peanuts can be effectively inhibited, and the using amount of chemical pesticides and the accumulation of the continuous cropping peanut in the soil can be reduced; in addition, the complex bacteria are significant in environment pollution; because the degraded product can be used as a carbon source by the bacteria and is converted into a harmless substance, and the carbon source of the soil is increased, so that the absorption of the crop root system to degradable substances is facilitated, and the pollution to the environment is avoided; the complex bacteria is high in stability; gene mutation is avoided after continuous culturing, the inheritance can be stabilized, the quantity of beneficial bacteria in the soil can be increased, and the soil fertility is increased; moreover, the preparation method of the complex bacteria is simple and an extraction process of the complex bacteria is simple; in addition, toxic substances are not added in the preparation process, and the complex bacteria are easy to use; the quality and the yield of crops such as peanuts in China can be improved.

The invention discloses a plum blossom planting method by grafting ripe plum blossom twigs onto twig stocks. The plum blossom planting method includes (1) preparing twigs, to be specific, selecting plum blossom twigs which are grown robustly in last year as scions, and storing the plum blossom twigs in sands in a moisturizing manner for grafting; (2) performing stock twig matching; (3) preparing the stocks, to be specific, after grafted in March, the stocks sprout with diameter 0.5cm-1cm to be subjected to grafting if part thereof fails to graft; (4) performing the cleft grafting, to be specific, two sides of the each scion are cut into slope types, then each scion is inserted in grafting cutting mouth, with two sides forming layer alignment; (5) performing management after the grafting, to be specific, pinching is performed timely to promote forking, and early flower bud differentiation is achieved. According to the arrangement, the ripe plum blossom twigs are adopted, leaf buds are full, sprouting is early, growing period is long, growth is enriched, and lignification by sprouting is realized to allow easy overwintering.

The invention discloses a method for cultivating a Monascus strain for highly yielding Monacolin K. The method comprises the following steps: separating and purifying red yeast rice to obtain a Monascus colony, adding the prepared Monascus colony to a medium prepared from agar, a potato juice, a mallet juice, lactic acid, anhydrous ethanol, sodium nitrate and potassium dihydrogen phosphate to prepare Monascus spores, flushing the medium with sterile sodium chloride water to obtain a Monascus spore bacterium suspension, performing ultraviolet irradiation on the Monascus spore bacterium suspension to achieve physical mutagenesis, adding a diethyl sulfate solution to achieve further mutagenesis, and mixing the obtained Monascus spore bacterium suspension with rice flour to rapidly breed the Monascus strain. An atomic irradiation mutagenesis technology is combined with a high-temperature piling cultivation technology, so the breeding rate of the Monascus strain in the medium is effectivelyincreased, the number of the Monascus strain is increased, and the metabolic Monacolin K is also increased, thereby the yield of the Monacolin K is increased.

The invention discloses a method for stereoscopically breeding earthworms under tree crowns. The method is characterized by including the steps: 1) ditching: digging a ditch with the width of 35-50cmand the depth of 25-35cm between tree rows of an economic forest; 2) filling: filling the ditch with fermentation rotten mixed breeding materials to reach the thickness of 15-25 centimeters; 3) watering: watering the mixed breeding materials completely filled in the ditch until overflow of water; 4) breeding of the earthworms: placing 215-225kg of earthworms into the thoroughly-watered mixed breeding materials per mu; 5) covering of straws: covering the mixed breeding materials with the straws. According to the method, the earthworms can effectively grow and develop, reproduction rate is increased by 20% or more, the yield of fruit trees is improved by 20-38%, and sugar content is improved by 1-5%.

The invention discloses a culturing method of plant half bud or one leaf quick breeding, which comprises the following steps: adopting half tender bud or one leaf of current year woody plant of half lignifications; or adopting herb tender stem with leaf as plug-in ear; treating with sterilizing liquid and rooting powder; cutting into slotter; spraying one time permeable immediately; entering into automatic control spraying management; spraying sterilizing liquid at following day morning or night; avoiding infection; interval-spraying sterilizing liquid; spraying phosphate fertilizer after rooting; accelerating lignifications of root system; getting reviving strain through 4-11 days. This invention does not need seed and grafting, which increases breeding rate and decreases breeding cost.

The invention belongs to the technical field of bacterial culture, and particularly relates to a magnetotactic bacteria culture machine. The magnetotactic bacteria culture machine comprises a box body, a box cover, a shaking module, a clamping module and a liquid adding module, wherein the box body is a rectangular no-cover box body; a left groove and a right groove are symmetrically formed in a left side wall and a right side wall of the box body; the box cover is horizontally mounted on the top of the box body; the shaking module is arranged on an inner bottom layer of the box body, and is used for shaking magnetotactic bacteria to promote a culture process; the clamping module is arranged above the shaking module, and is used for clamping a device for accommodating the magnetotactic bacteria; the liquid adding module is arranged above the clamping module, and is used for adding magnetotactic bacteria culture liquid into the box body. According to the magnetotactic bacteria culture machine disclosed by the invention, through mutual cooperation of the shaking module and the clamping module, the magnetotactic bacteria culture liquid is uniformly shaken and mixed, so that full reaction is carried out; then, through the liquid adding module, replacement of the bacteria culture liquid is realized, so that the culture efficiency of the magnetotactic bacteria is greatly improved.

The invention discloses a culture medium box set for facilitating crgotvaunilocularis in-vitro rapid propagation. The culture medium box set comprises a culture medium 1 for primary culture, a culture medium 2 for secondary culture, a culture medium 3 for toughening seedlings and a culture medium 4 for taking roots, wherein the culture medium 1 for primary culture takes an MS (murashige and skoog) culture medium as a basal culture medium, 0.5-2mg / L of BA (butyl acrylate), 0.1mg / L of NAA (naphthalene acetic acid), 0-0.1mg / L of TDZ (thidiazuron), 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium, the culture medium 2 for secondary culture takes an MS culture medium as a basal culture medium, 0.5mg / L of BA, 0-0.1mg / L of NAA, 0-0.5mg / L of GA3 (gibberellin), 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium, the culture medium 3 for toughening seedlings takes an MS culture medium as a basal culture medium, 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium, the culture medium 4 for taking roots takes 1 / 2 MS culture medium as a basal culture medium, and 0.5-1.5 mg / L of IBA (indole-butyric acid), 25-35mg / L of sucrose and 6.5-7.5 mg / L of agar are added into the culture medium. The invention further discloses a method for facilitating crgotvaunilocularis in-vitro rapid propagation by the culture medium box set. Crgotvaunilocularis is cultured by the method under optimized conditions, and ideal reproduction rate, rooting rate and transplanting survival rate can be achieved.

The invention relates to water purification equipment. The equipment includes a barrel body, one end of a water inlet pipe communicated with the barrel body extends to the water bottom, a water outletof a submersible pump installed in the barrel body is communicated with a dissolved air tank, before pumping, the submersible pump utilizes the negative pressure effect of fluid to suck in the air, water flow passes through two layers of microbial filler in the barrel body and then is communicated with the water outlet, and the equipment is suitable for sewage treatment upgrading, and can be applied to aerobic contact oxidation tank, lake and river treatment. Through the above settings, water is pumped from the bottom of a water area, so that the water can achieve the effect that flowing water does not rot. Air and water flow and are mixed, then the mixture enters the pressure dissolved air tank, not only rapidly increases dissolved oxygen, but also increases the treatment effect of microbial contact oxidation. Also the way utilizes the submersible pump to replace an aeration pump, while realizing hierarchical flow and mutual replacement of the water, realizes oxygen increasing, can save energy up to 80%, and has the effects of reducing energy consumption and cost. The submersible pump does not generate noise and has good heat dissipation effect.

The invention discloses a microbe alternate grouting device for soft soil stabilization and a using method. The microbe alternate grouting device comprises an air suction pipe, wherein a plurality ofair suction holes are formed in the outer wall of the air suction pipe; a grouting pipe and the air suction pipe are connected into one stabilization pipeline through a partition plate, and the outerwall of the lower section of the stabilization pipeline is wrapped with non-woven fabric; the upper end of the grouting pipe communicates with a slurry tank, and the upper end of the air suction pipeis connected with a water-air separator and a vacuum pump; and a connector is arranged at the lower end of the stabilization pipeline, a flow channel is arranged in the middle part of the connector, and a downward pressing block is connected to the lower end of the connector and provided with a plurality of slurry spraying channels. Alternate grouting is carried out through the grouting device, large-area grouting stabilization of soil mass can be rapidly achieved while the slurry using amount is greatly reduced, the grouting device starts the vacuum pump at the same time after grouting stabilization of the soil mass is completed, high-concentration NH4Cl generated through a reaction is pumped out and loaded into a specific container to be conveyed to a sewage treatment plant to be treated, and therefore soil and underground water can be prevented from being polluted.

The invention discloses a cherax quadricarinatus circulating water breeding system and method. The circulating water breeding system comprises a high-position water storage pond, a subsurface flow-vertical upward flow composite artificial wetland, a water quality restoration pond, a chlorella expanding culture bucket, a composite effective bacteria expanding culture bucket, a parallel breeding pond, a drainage channel, a water lifting inspection well and a water lifting pump which are all positioned in a heat-insulating greenhouse, the high-position water storage pond, the subsurface flow-vertical upward flow composite artificial wetland, the water quality restoration pond, the parallel breeding pond, the water drainage channel and the water lifting inspection well are sequentially communicated, the water lifting pump is used for lifting supernate in the water lifting inspection well to the high-position water storage pond, and the chlorella expanding culture bucket and the composite effective bacteria expanding culture bucket are communicated with the water quality restoration pond and controlled to be open and closed through valves. By the system, shrimp seed breeding rate is increased, shrimp seeds are synchronous in specification, supply of high-quality shrimp seeds is increased greatly, and development of cherax quadricarinatus breeding industry is promoted.

The invention discloses an aseptic germinating and rapid propagating method for hibiscus aridicola anthony seeds. According to the aseptic germinating and rapid propagating method for the hibiscus aridicola anthony seeds, by taking mature hibiscus aridicola anthony seeds as explants, and performing a series of cultivation steps of sterilizing the explants, performing aseptic germinating on the seeds, proliferating and rooting, the problems about artificial reproduction, introduction and domestication, and development and utilization of the hibiscus aridicola anthony in landscape gardening areeffectively solved, and the problems that the number of natural plants is reduced and a natural vegetation area is destructed because of excessive utilization of wild resources are effectively avoided; the aseptic germinating and rapid propagating method for the hibiscus aridicola anthony seeds has important significance in the aspect of development and utilization. According to the method provided by the invention, the seeds start to germinate within 20 days; the aseptic germinating rate is 60 percent; the reproduction period is 30 days; the proliferation coefficient is 5; the rooting rate is98 percent; the transplanting survival rate is 95 percent; the proliferation number and the growth rate of the hibiscus aridicola anthony are greatly improved; technical support is provided for the introduction and domestication, gardening development, storage and scale production of species.

The invention discloses a vacuum grouting device for microbial seepage prevention of a jointed rock mass and a using method thereof. A plurality of suction holes are formed in an outer wall of a lowersection of a suction pipe, a grouting pipe is arranged in the suction pipe, an upper end of the suction pipe extends upwards, a nut, a spacer and a stop-grouting plug are sleeved on the outer wall ofthe upper section of the suction pipe, a non-woven fabric is arranged on the outer wall of the lower section of the suction pipe, and an exhaust pipe is arranged on the side wall of the upper sectionof the suction pipe; three grouting holes are drilled when a rock mass is subjected to grouting anti-seepage; and after grouting devices are placed in the grouting holes, the three grouting devices alternately perform grouting. The vacuum grouting device adopts vacuum negative pressure grouting. For the jointed rock mass, good effects are hard to be achieved by using common intravenous grouting and other traditional methods. With the aid of vacuum negative pressure, the original air and water in the rock mass that hinder the advancement of the grout are removed; at the same time, the tiny airbubbles in fissures are sucked and discharged together, so that the effective contact between a bacterial liquid the rock particles is more, so as to improve anti-seepage effect.

The invention discloses a culture medium box set and method for in-vitro rapid propagation of scaevola taccada. The culture medium box set for in-vitro rapid propagation of scaevola taccada comprisesa scaevola taccada cluster bud induction culture medium, a somatic embryo and adventitious bud induction culture medium, a somatic embryo and adventitious bud proliferation and elongation culture medium, a scaevola taccada rooting induction culture medium and river sand used as a scaevola taccada tissue culture seedling transplanting medium. Moreover, an efficient scaevola taccada tissue culture seedling rapid propagation technology system utilizing the box set is also built. A basis is provided for protecting, developing and utilizing scaevola taccada resources, and a blank of scaevola taccada in the aspect of the tissue culture rapid propagation technology is filled in.

The invention discloses a fertilizing method for amorphophllus planting, and belongs to the technical field of agricultural planting. The fertilizing method for amorphophllus planting comprises the following steps that firstly, a non-woven bag is made, then the non-woven bag is placed on the planting ground, and organic fertilizer is applied in the non-woven bag; then seed amorphophllus is placedon the applied organic fertilizer, and then the organic fertilizer is used for covering the seed amorphophllus; then the non-woven bag is covered with soil to form a ridge to complete the applicationof base fertilizer; and the organic fertilizer is prepared from the following components: edible fungus residue, nitrogen fertilizer, potash fertilizer, insecticide and soil. The fertilizing method can play the moisture-proof and breathable roles by planting the amorphophllus in the non-woven bag and by applying the organic fertilizer in the non-woven bag, thereby facilitating the growth of an amorphophllus root system and effectively increasing the expansion coefficient of the amorphophllus and reducing the incidence rate of amorphophllus lesions.

The invention belongs to the technical field of microorganism culture, and particularly relates to a kluyveromyces marxianus strain culture medium and a strain culture method thereof. The kluyveromyces marxianus strain culture medium comprises a base, a box body and a motor, wherein the box body is designed to be in a disc shape; the base is fixedly connected with the bottom of the box body; a culture cavity is formed in the box body; the motor is fixedly connected to one side of the box body; an output shaft of the motor penetrates through the box body and extends into the culture cavity; a rotating wheel is fixedly connected to one end positioned in the culture cavity, of the output shaft of the motor; and an annular groove is formed in one side close to the motor, of the culture cavity.By increasing air pressure in a feeding box, a liquid culture medium drips into the culture cavity through the micropores under the action of pressure and gravity and falls into culture dishes on bearing plates in the dripping process in the culture cavity, and liquid drops carry dissolved oxygen and nutrient substances to drip into the culture dishes. Nutrients and oxygen are provided for kluyveromyces marxianus strains in the culture dishes.

The invention relates to the technical field of plant tissue culture and provides a tissue-culture rapid propagation method for craigia yunnanensis. According to the tissue-culture rapid propagation method, induction and differentiation culture are integrated, multiplication and subculture are integrated, and seedling strengthening and rooting culture are integrated, so that the culture method issimplified. The tissue-culture rapid propagation method has advantages that the problem of difficulty in short-time mass propagation, storage and sustainable utilization due to less natural resourcesof craigia yunnanensis can be solved, a biotechnological research gap of craigia yunnanensis is filled, and a foundation is laid for comprehensive development and sustainable utilization of craigia yunnanensis.

The invention discloses a high-survival-rate grafting method for carya illinoensis. The method is characterized by comprising the steps: rootstocks for being grafted are nursery-grown plants cultured from carya illinoensis seeds, and a rootstock treatment mode is transplanting; branches, which are robust in growth and plump in bud, are selected from excellent single plants and are subjected to wax sealing treatment, and scions are grafted while the scions are picked; grafted parts of the rootstocks are trimmed off during grafting, full and plump buds of middle-lower parts of the scions are selected as scion buds, grafting is carried out by adopting a bud-sticking grafting method, lower ends of the scion buds are aligned with cuts of the rootstocks, and plastic films are used for carrying out binding for moisture preserving; and in a scion and rootstock healing stage, the temperature and humidity of a greenhouse are controlled, and sunshine direct-sunning is avoided until the scions and the rootstocks are completely healed and grafted seedlings survive.

The invention provides a tissue culture and rapid propagation method for manglietiastrum sinicum, and belongs to the technical field of plant tissue culture. According to the method, the tender terminal buds or lateral buds of the sterilized manglietiastrum sinicum are used as explants; through a series of culture including induction, differentiation, proliferation and rooting in sequence on a specific culture medium, complete manglietiastrum sinicum plants are successfully propagated, and the problems of tissue culture and rapid propagation, introduction and domestication, protection, and development and utilization of scientific research and landscaping of the manglietiastrum sinicum are effectively solved; and meanwhile, natural disappearance and death of wild resources are effectivelyavoided, the population quantity in the nature is maintained, and the method plays a positive role in the aspects of seedlings, required by ex-situ protection, near-situ protection and in-situ protection, of the manglietiastrum sinicum. According to the method, the induced differentiation rate is 58%, the propagation period is 60 days, the propagation coefficient is 3.6, the rooting rate is 81%, the transplanting survival rate is 86% or above, the propagation number and the growth rate of the manglietiastrum sinicum are greatly increased, and a technical support is provided for protection andpropagation, introduction and domestication, storage and large-scale production of the manglietiastrum sinicum.