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Geobacillus sp. UTM02 and application thereof

A Geobacillus and bacteria agent technology, applied to the thermophilic Geobacillus strain UTM02 and its application fields, can solve the problems of high energy consumption, unfavorable preservation and transportation, and the decrease of viable bacteria count, so as to improve the moisture content of materials and facilitate preservation With the effect of convenient transportation and operation

Active Publication Date: 2012-09-12
北京绿源科创环境技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, most of the current commercial bacterial preparations are liquid, which is not conducive to storage and transportation. When the storage period is longer than 3 months, the number of viable bacteria will decrease significantly. Moreover, the control of fermentation temperature usually requires additional energy, and the energy consumption is relatively large.

Method used

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  • Geobacillus sp. UTM02 and application thereof
  • Geobacillus sp. UTM02 and application thereof
  • Geobacillus sp. UTM02 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and identification of Geobacillus thermophile UTM02

[0030] In a sterile environment, weigh 5g of the sludge compost sample into a 50ml sterile water triangular flask with 10 glass beads, place it on a shaker and vibrate at 160r / min for 30min to fully disperse the sample, and then place it at 70°C , and cultured statically for 6 hours.

[0031] Another 10g sludge compost sample was weighed in a beaker with 200ml distilled water, stirred on a magnetic stirrer for 1 hour, then filtered with filter paper to get the filtrate. According to the formula, 0.5g beef extract, 1g peptone, 0.5g sodium chloride, 100ml filtrate, pH 7.2-7.4, prepare bacterial filtrate medium, and sterilize at 121°C for 20min.

[0032] Take out the above-mentioned triangular flask for static culture, and let it stand for 3 minutes in a sterile environment, then use a sterile pipette to draw 10ml of supernatant and add it to 100ml of sterilized bacterial filtrate medium, shake well...

Embodiment 2

[0038] The preparation of embodiment 2 compost inoculants

[0039] (1) Slope activation: Take the slant of the preserved strains and make multiple streaks on the slant for activation and culture at 65°C until abundant colonies are formed;

[0040] (2) Shake flask seed solution preparation: add decomposed sludge compost water extract with extraction ratio of 1:10 to the bacterial culture solution by 5% (v / v), and place it in a conical flask for sterilization, and wait for cooling To 50°C, inoculate a slant bacterial colony after activation culture, place it in a constant temperature water bath shaker, shake and cultivate at 70°C for 24 hours, and shake at a frequency of 160rpm;

[0041] (3) Preparation of fermented seed liquid: the shake flask seeds are inserted into the seed tank equipped with sterilized bacterial culture solution at 10% (v / v), the temperature is controlled at 65° C., and the ventilation is performed for 30 minutes at intervals of 6 hours, and the ventilation ...

Embodiment 3

[0048] The preparation of embodiment 3 compost inoculants

[0049] (1) Slope activation: take the slant of the strain, mark the line on the slant and activate it at 60°C until a rich colony is formed;

[0050] (2) Shake flask seed solution preparation: add decomposed sludge compost water extract with extraction ratio of 1:20 to the bacterial culture solution by (v / v) 10%, and place it in a conical flask for sterilization, and wait for cooling , inoculate a slant colony after activation culture, place it in a constant temperature water bath shaker, shake it at 65°C for 48 hours, and shake it at a frequency of 200rpm;

[0051] (3) Preparation of fermented seed liquid: the shake flask seeds are inserted into the seed tank equipped with sterilized bacterial culture solution at 3% (v / v), controlled temperature is 70°C, and ventilated for 20min at intervals of 5 hours, and the ventilation rate is 0.5:1 ( v / v), stirring at 200 rpm, and culturing for 4 days.

[0052] The formula of ...

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Abstract

The invention provides a geobacillus sp. strain UTM02. The storage number of the geobacillus sp. strain UTM02 is CGMCC No.5642. The geobacillus sp. strain UTM02 can rapidly multiply at the temperature of 70 DEG C. The invention further provides solid compost inoculant prepared from the geobacillus sp. strain UTM02, and a preparation method and application of the solid compost inoculant. The compost inoculant is low in production cost, stable in quality and convenient to operation, can be applied to high-temperature sludge composting, does not affect moisture content of compost bodies, is environment-friendly and pollution-free, has excellent performances, achieves double effects of thermal energy utilization and waste treatment, and has good economic and social benefits.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a thermophilic Geobacillus strain UTM02 and its application. Background technique [0002] Thermophiles are a type of microbial flora that can grow and maintain biodegradation activity under high temperature conditions. Microorganisms with a maximum growth temperature higher than 60°C and an optimum growth temperature higher than 50°C are called thermophilic bacteria, while microorganisms with a maximum growth temperature higher than 90°C and an optimum growth temperature higher than 65°C are called extremophilic bacteria. Heat (Hyper-thermophilic) bacteria. The enzyme protein contained in the cells of thermophilic bacteria has a unique heat resistance and a compatible molecular structure, so it has great application value in the development and utilization of genetic engineering, protein engineering, and mineral resources. [0003] During the composting pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/01
CPCY02W30/40
Inventor 刘永跃周顺桂许宜北汪涌
Owner 北京绿源科创环境技术有限公司
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