Tissue culture and rapid propagation method for manglietiastrum sinicum

A culture medium and subculture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of narrow distribution area of ​​canopy wood, scarce population, insufficient seedlings, etc.

Active Publication Date: 2021-01-22
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for tissue culture and rapid propagation of Canopywood, which fills up the gap in the biotechnology of Canopywood, and simultaneously solves the problems of narrow distribution area of ​​Canopywood in the wild, few populations, and insufficient seedlings.

Method used

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  • Tissue culture and rapid propagation method for manglietiastrum sinicum
  • Tissue culture and rapid propagation method for manglietiastrum sinicum
  • Tissue culture and rapid propagation method for manglietiastrum sinicum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Screening method for inducing differentiation medium

[0046] Take the young terminal buds and side buds of Canopy wood as explants, soak them in 1% soapy water for 10 minutes, rinse them with running water, put them on the ultra-clean table, cut them into stems with single nodes, and use 75% Disinfect with alcohol for 10 seconds, rinse with sterile water for 3 times, disinfect the surface with 0.1% mercuric chloride solution for 10 minutes, wash with sterile water for 3 to 5 times, inoculate in the prepared canopy wood induction medium, 1 node per bottle.

[0047] Inoculate the sterilized stem segments in MS+3.0mg / l 6-BA+1.0mg / L IAA, MS+2.5mg / l 6-BA+1.0mg / L IAA, MS+2.0mg / l 6- BA+1.0mg / L IAA, MS+1.0mg / l 6-BA+1.0mg / L IAA, MS+0.5mg / l 6-BA+1.0mg / L IAA culture medium to induce differentiation, the MS The medium also includes 30 g / L sucrose, 5 g / L agar, and a pH value of 5.8. The culture temperature is 23-30° C., and the artificial light is assisted during the culture peri...

Embodiment 2

[0052] Proliferation and Subculture Media Screening Methods

[0053] Using MS medium as the basic medium for proliferation and subculture hormone screening, the cytokinin is 6-BA, and the concentration range is (0mg / L, 0.1mg / L, 0.5mg / L, 1.0mg / L, 1.5mg / L, 2mg / L, 6 concentration gradients), the auxins are all IAA, the concentrations are (0.1mg / L, 0.5mg / L, 1.0mg / L, 3 concentration gradients), and the uniform design method is used. The culture temperature is 23-30° C., the light time is 10 hours, and the light intensity is 1500 Lux.

[0054] The results are shown in Table 2.

[0055] Table 2 Proliferation and Subculture Medium Hormone Screening

[0056]

[0057] It can be seen from Table 2 that MS medium+1.0mg / L 6-BA+0.5mg / L IAA+sucrose 30g / L+agar 5g / L, pH value 5.8, culture period 60 days, light intensity 1500Lux, temperature 23-30 ℃. Count the side buds and terminal buds quantity that each propagule produces, the average value of bud node quantity (comprising terminal bu...

Embodiment 3

[0059] Screening method for strong seedling rooting medium

[0060] Using MS medium as the basic medium for hormone screening of strong seedlings and rooting medium, the concentration range of IBA is 0.1mg / L, 0.5mg / L, 1.0mg / L, 2mg / L 4 concentration gradients, and the concentration of IAA is 0.1 mg / L, 0.5mg / L, 1.0mg / L, 2.0mg / L 4 concentration gradients, using the uniform design method. The culture temperature is 23-30° C., the light time is 10 hours, and the light intensity is 1500 Lux.

[0061] The results are shown in Table 3.

[0062] Table 3 Rooting Medium Hormone Screening Results

[0063]

[0064] As can be seen from Table 3, MS medium+1.0mg / L IAA+1.0mg / L IBA+sucrose 30g / L+agar 5g / L, pH value 5.8, culture period 30 days, rooting rate reached 81%.

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Abstract

The invention provides a tissue culture and rapid propagation method for manglietiastrum sinicum, and belongs to the technical field of plant tissue culture. According to the method, the tender terminal buds or lateral buds of the sterilized manglietiastrum sinicum are used as explants; through a series of culture including induction, differentiation, proliferation and rooting in sequence on a specific culture medium, complete manglietiastrum sinicum plants are successfully propagated, and the problems of tissue culture and rapid propagation, introduction and domestication, protection, and development and utilization of scientific research and landscaping of the manglietiastrum sinicum are effectively solved; and meanwhile, natural disappearance and death of wild resources are effectivelyavoided, the population quantity in the nature is maintained, and the method plays a positive role in the aspects of seedlings, required by ex-situ protection, near-situ protection and in-situ protection, of the manglietiastrum sinicum. According to the method, the induced differentiation rate is 58%, the propagation period is 60 days, the propagation coefficient is 3.6, the rooting rate is 81%, the transplanting survival rate is 86% or above, the propagation number and the growth rate of the manglietiastrum sinicum are greatly increased, and a technical support is provided for protection andpropagation, introduction and domestication, storage and large-scale production of the manglietiastrum sinicum.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rapid propagation method for tissue culture of canopy wood. Background technique [0002] Manglietiastrum sinicum belongs to the Magnoliaceae genus. It is an evergreen tree with a height of about 40 meters and a diameter at breast height of 1.2 meters. It is a unique species in Yunnan, China. It originated 140 million years ago and is the oldest in the Magnoliaceae. One of the single species of plants, it is named for its straight and smooth trunk and huge crown. Huagai wood is an ancient relic tree species left over from the Tertiary and Quaternary geological ages. In 1999, it was listed as a national first-level key protected wild plant, and was listed as a critically endangered species by the International Union for Conservation of Nature (IUCN) Global Red List. It is a typical extremely small population wild plant. It was first discovered in Fadou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗桂芬孙卫邦陶丽丹葛佳蔡磊
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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