Tissue culturing method for quercus virginiana

Abstract

A tissue culturing method for quercus virginiana belongs to plant tissue culturing methods and is characterized by comprising the following steps: 1 selecting of explants; 2 disinfection of the explants; 3 primary culture; 4 multiplication culturing of adventitious buds; 5 culturing of strong seedlings; 6 culturing of strike roots; and 7 seedling acclimatization and transplanting. The tissue culturing method for the quercus virginiana can keep stress resistance and enjoyment of a female parent and can effectively increase propagation coefficient of the quercus virginiana. Compared with seminal propagation, tissue culturing reduces dependency on imported seeds, can effectively reduce nursery stock cost, and saves expenditures. Compared with cutting propagation, the tissue culturing is short in seedling culturing period, can obtain the nursery stock with strike roots within 4 months, and saves cost.

Description

technical field

[0001] The invention belongs to a plant tissue culture method, in particular to a Virginia oak tissue culture method. Background technique

[0002] Virginia oak ( Quercus virginiana) It belongs to the Fagaceae (Fagaceae) species of the genus Quercus (Quercus), native to the United States, and is an evergreen broad-leaved tall tree. Single leaf, alternate, old leaves are leathery, the surface of the leaves is dark green and shiny, the back is green and white, the leaf color, shape, size, texture and other traits have strong plasticity with the influence of developmental stage and environment, the new leaves are large and fleshy, the color Light green, leaf margin teeth are large and few; old leaves are small and leathery, leaf margin teeth are small and many. It blooms in early spring, the flowers are yellow, and the mature fruit is brown. It is commonly known as acorn, which provides a rich source of food for birds and animals. It falls after maturity, and ...

Examples

Embodiment 1

[0032] Embodiment 1: a kind of Virginia oak tissue culture method, its cultivation step is as follows:

[0033] (1) Selection of explants: The explants were collected from plants cultivated in the Stress Resistance Physiological and Ecological Laboratory of Yalin Institute, Chinese Academy of Forestry.

[0034] (2) Disinfection of explants: Take the stem section with buds as explants, wash the explants with tap water for 12 hours, and then disinfect them with 70-75% alcohol for 30 seconds (if the explants are collected in the field, add 1-2 drops of Tween 80 to strengthen the penetration of the disinfectant), then soak in mercuric acid for 3-5 minutes, then rinse with sterile water several times, and put it in a sterile glass bottle for later use.

[0035] (3) Primary culture: cut the sterilized explants into about 1 cm bud stem segments and insert them into WPM or 1 / 4MS+growth regulator substance 6-benzyl adenine 1.0 mg / L medium for 30 days, and give light for about 3000lx, ...

technical field

[0001] The invention belongs to a plant tissue culture method, in particular to a Virginia oak tissue culture method. Background technique

[0002] Virginia oak ( Quercus virginiana) It belongs to the Fagaceae (Fagaceae) species of the genus Quercus (Quercus), native to the United States, and is an evergreen broad-leaved tall tree. Single leaf, alternate, old leaves are leathery, the surface of the leaves is dark green and shiny, the back is green and white, the leaf color, shape, size, texture and other traits have strong plasticity with the influence of developmental stage and environment, the new leaves are large and fleshy, the color Light green, leaf margin teeth are large and few; old leaves are small and leathery, leaf margin teeth are small and many. It blooms in early spring, the flowers are yellow, and the mature fruit is brown. It is commonly known as acorn, which provides a rich source of food for birds and animals. It falls after maturity, and ...

Examples

Embodiment 1

[0032] Embodiment 1: a kind of Virginia oak tissue culture method, its cultivation step is as follows:

[0033] (1) Selection of explants: The explants were collected from plants cultivated in the Stress Resistance Physiological and Ecological Laboratory of Yalin Institute, Chinese Academy of Forestry.

[0034] (2) Disinfection of explants: Take the stem section with buds as explants, wash the explants with tap water for 12 hours, and then disinfect them with 70-75% alcohol for 30 seconds (if the explants are collected in the field, add 1-2 drops of Tween 80 to strengthen the penetration of the disinfectant), then soak in mercuric acid for 3-5 minutes, then rinse with sterile water several times, and put it in a sterile glass bottle for later use.

[0035] (3) Primary culture: cut the sterilized explants into about 1 cm bud stem segments and insert them into WPM or 1 / 4MS+growth regulator substance 6-benzyl adenine 1.0 mg / L medium for 30 days, and give light for about 3000lx, ...