The invention is suitable for the field of
magnoliaceae magnolia
plant cultivation, and discloses a breeding method for
tissue culture of axillary buds of magnolia sinostellata. The breeding method comprises the following steps: explant selection, explant treatment, anti-
browning treatment,
primary culture, subculture, rooting culture and
seedling transplantation, wherein a culture medium for
primary culture comprises an MS basic culture medium, 0.5-2 mg / L of 6-BA, 0.2-1 mg / L of KT, 0.05-0.2 mg / L of NAA, 0.05-0.1 mg / L of IBA, 7 g / L of
agar and 30 g / L of
cane sugar, and the pH value of the
primary culture medium is 5.8-6.5; a culture medium for subculture comprises the MS basic culture medium, 0.5-1.5 mg / L of 6-BA, 0.05-0.2 mg / L of NAA, 0.05-0.1 mg / L of IBA, 7 g / L of
agar and 30 g / L of canesugar, and the pH value of the subculture medium is 5.8-6.5; and a culture medium for rooting culture comprises a 1 / 2MS basic culture medium, 1-2 mg / L of NAA, 1-2 mg / L of IBA, 7 g / L of
agar and 30 g / L of
cane sugar, and the pH value of the rooting culture medium is 5.8-6.5. According to the breeding method for
tissue culture of the axillary buds of the magnolia sinostellata, the propagation speedof the magnolia sinostellata can be increased, and the obtained magnolia sinostellata seedlings can maintain the characteristics of mother seeds.