912 results about "Planting seed" patented technology

The invention provides a strain of Burkholderia graminicola as well as a bacterial agent and application of the Burkholderia graminicola. The preservation number of the Burkholderia graminicola is CCTCC (China Center For Type Culture Collection) NO: M20242399. The Burkholderia paragallinarum provided by the invention has the functions of nitrogen fixation, phosphate solubilization, siderophore production and indoleacetic acid production. The Burkholderia grassy and the fungicide taking the Burkholderia grassy as the effective active component can promote the germination of plant seeds and the growth of crops.

The invention discloses an application of a TaNLP-3B gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaNLP-3B is cloned from wheat, the gene TaNLP-3B is overexpressed in wheat Fielder to obtain a TaNLP-3B transgenic overexpressed plant, and a detection result shows that the protein content in wheat seeds can be remarkably increased by more than 40% through TaNLP-3B gene overexpression, so that the TaNLP-3B gene has an effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be increased by more than 40%. The method can be applied to plant breeding or quality improvement.

The invention discloses a method for improving pasture productivity by optimizing a mixed sowing proportion and a sowing mode, which comprises the following steps of: performing rotary tillage on soil and applying humus before freezing in winter, turning over the soil and applying a base fertilizer after reviving in spring, then selecting leguminous plants and gramineous plants according to a weight ratio of 3: 7, respectively and uniformly mixing seeds of two functional groups, the method comprises the following steps: ditching, ridging and sowing gramineous plant seeds in line, sowing leguminous plant seeds in holes between two rows of gramineous plants 20-30 days after the gramineous plant seeds emerge, carrying out topdressing and pest and weed damage management after sowing, mowing the overground part of the grassland at the last stage of plant growth, and controlling the stubble height of the leguminous plants and the gramineous plants. By accurately optimizing the mixed sowing proportion and sowing strategy of leguminous and gramineous pasture and combining the complementarity of plant functional groups in ecological niche (time and space), an innovative pasture mixed sowing method is provided, and theoretical support and technical guarantee are provided for efficient and sustainable development of animal husbandry production.

The invention discloses an application of a TaPKMT-5A gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaPKMT-5A is cloned from wheat, the gene TaPKMT-5A is overexpressed in wheat Fielder, a TaPKMT-5A gene overexpressed plant is obtained, and a detection result shows that the protein content in wheat seeds can be remarkably increased by 13.3%-16.9% or above through TaPKMT-5A gene overexpression, so that the TaPKMT-5A gene has the effect of regulating and controlling the protein content in the plant seeds, and the protein content in the wheat seeds can be remarkably increased by 13.3%-16.9% or above. The method can be applied to plant breeding or quality improvement.

Seed treatment systems with a mixing reservoir including a tank, a circulation mechanism positioned within the tank, and a controller coupled to the circulation mechanism, where the circulation mechanism includes a plurality of vanes configured to rotate about an axis within the tank, and where the controller is configured to mix seed treatment components within the tank in one or more mixing cycles by rotating the plurality of vanes within the tank, each mixing cycle including a first mixing operation in which the plurality of vanes rotate in a first direction about an axis for a first time period, and a second mixing operation in which the plurality of vanes rotate in a second direction about the axis opposite to the first direction for a second time period. Methods of use of the disclosed systems, e.g., for treating plant seeds, and agricultural compositions produced thereby are also provided.

Methods for stimulating plant growth and / or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and / or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

The invention discloses a loess slope ecological protection vegetation substrate and a preparation method and a performance detection method thereof, and belongs to the technical field of slope ecological protection. The invention solves the problem of how to develop a novel slope ecological protection base material which is ecological, environment-friendly, good in vegetation effect and low in cost. The soil comprises a base material and plant seeds mixed in the base material, the use amount of the plant seeds is 20-30 g / m < 2 >, and the base material comprises the following components in parts by weight: 100-110 parts of loess, 20-30 parts of bentonite, 20-30 parts of bentonite, 20-30 parts of bentonite, 10- 4 to 10 parts of bentonite; 0.1 to 0.3 part of basalt fiber; 0.2 to 0.4 part of a water-retaining agent; 0.8-1.2 parts of an organic fertilizer; 0.5-0.8 part of a compound fertilizer; 2-3 parts of a microbial fertilizer; and 44-48 parts of water. Bentonite is used for replacing cement and other harmful binders, a natural soil system of the base material is maintained, the base material is more environmentally friendly, meanwhile, the water retention capacity of the base material is improved, the pH value of the base material does not need to be adjusted, plant growth is facilitated, the air permeability of the base material is improved through basalt fibers, the shearing performance, the crack resistance and the water stability of the base material are improved, and the service life of the base material is prolonged. And a stable growth environment is provided for vegetation.

The invention relates to a saline-alkaline tolerant microbial seed coating agent and application thereof. The seed coating agent comprises a growth-promoting rhizobacteria inoculant and an auxiliary agent, the volume ratio of the growth-promoting rhizobacteria inoculant to the auxiliary agent is 1: (1-100); in each gram of the seed coating agent, the viable count of the growth-promoting rhizosphere bacteria is (1-100) * 10 < 10 > CFU; the growth-promoting rhizobacteria is selected from one or more of pseudomonas stutzeri A1501, pseudomonas fluorescens ATCC 13525, bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703, and the growth-promoting rhizobacteria is selected from one or more of bacillus amyloliquefaciens FZB42 and azotobacter chroococcum BNCC336703; on the basis of the total weight of the auxiliary agent, the auxiliary agent is prepared from the following components in percentage by weight: 1 to 9 percent of film forming agent, 1 to 9 percent of protective agent, 0.0001 to 0.001 percent of regulator, 0.0001 to 0.001 percent of active agent, 0 to 25 percent of filler and 58 to 97 percent of water. The seed coating agent can improve the rhizosphere colonization ability and survival rate of rhizosphere growth-promoting bacteria, can also improve the saline-alkaline tolerance of plant seeds, and effectively promotes plant growth.

The invention relates to the technical field of ecological restoration, in particular to a preparation method of novel vegetation concrete greening additive AB bacteria, which comprises the following steps: step (1) screening and activating double bacteria; (2) preparing an inner core structure; (3) loading double bacteria; (4) coating a shell layer; (5) loading a functional matrix; and (6) preparing the vegetation concrete greening additive AB bacteria. According to the method, a multi-layer and function-synergistic microorganism-seed-carrier composite system is constructed and used for surface concrete attachment of the high steep slope, and a plant-microorganism symbiotic system with physical isolation, chemical buffering and nutrition self-sustaining capacities is actively constructed for plant seeds and functional microorganisms in a strongly alkaline macroscopic environment; and finally, the long-acting ecological stability of the high and steep slope is realized.

The invention relates to the technical field of agricultural micro-ecological application, and particularly discloses a composite combined inoculation method using arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, which comprises the following steps: selecting corn plant seeds without plant diseases and insect pests; a corn planting land is randomly divided into land parcels with the area of 6 m < 2 > (2 m * 3 m), a buffer zone of 1.0 m is arranged between every two adjacent land parcels, and the cultivation mode is flat cropping; the method comprises the following steps: planting arbuscular mycorrhizal fungi inoculum comprising mycorrhiza, dry sand containing spores and ectotrophic hyphae and corn seeds in soil by using a seeder during corn sowing, and after the corn is planted for 30 days, inoculating the plant growth-promoting rhizobacteria inoculum into plants and soil by using a flood irrigation root irrigation method, when the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are inoculated together, the corn rhizosphere microbial community can be changed, the richness and diversity of fungi and bacterial communities in soil are increased, and the relative abundance of plant pathogens in the soil is reduced.

The invention discloses a riverway dredging method which comprises the following steps: dynamically scanning a riverway along a spiral propulsion path, and obtaining riverbed topographic data and sediment thickness in real time; a three-dimensional riverbed model is established based on the riverbed topographic data, and a core dredging area, a transition maintenance area and an ecological reserve area are divided in the three-dimensional riverbed model according to the sediment thickness; the core dredging area is divided into a surface layer, a middle layer and a bottom layer in the vertical direction, and a differentiated dredging process is executed; carrying out mud-water separation treatment on the dredged material, purifying the separated water, and reinjecting the purified water into the river channel; prefabricated ecological concrete modules are laid on the surface of a dredged riverbed, diversion trenches and biological nests are formed, and the biological nests are filled with polylactic acid / straw composite fiber felts containing local submerged plant seeds. Through spiral scanning, three-dimensional modeling and intelligent partitioning, integration of monitoring, planning, construction and repairing is achieved, accurate dredging is implemented, and the dredging efficiency is improved.

The invention discloses a pesticide slow-release granule suitable for simultaneous sowing of pesticide and plant seeds. The pesticide sustained-release granule has double coating layers, the first coating layer takes a pesticide effective component A as a core and a nano calcium carbonate carrier material as a shell, and nano calcium carbonate is used for carrying out first-layer coating on a bactericide effective component through a physical adsorption method; the second coating layer is formed by mixing the first coating layer and the pesticide active ingredient B as a core and taking materials such as attapulgite as a shell. The sustained-release granule has good sustained-release performance, can stably release effective components in a long time, prolongs the pesticide effect period, reduces frequent pesticide application operation, and further improves the pesticide utilization rate.

The present invention relates to a strain of Glutamicibacter endophyticus subsp. halotolerans. The strain Glutamicibacter endophyticus NY3-1 of Glutamicibacter endophyticus subsp. halotolerans is deposited in the China Center for Type Culture Collection with the deposit number CCTCC No: M20242321. The 16S rDNA nucleotide sequence of the strain Glutamicibacter endophyticus NY3-1 is shown as SEQ ID NO: 1. This strain can metabolize normally in both salt-free and salt-containing environments, and can tolerate environments with a pH value of 6-11. It has the effects of improving the germination rate of plant seeds and promoting plant growth in both salt-containing and salt-free environments. Moreover, this strain can improve the soil environment of saline-alkali land, has broad application prospects, and provides a new biological resource for the biological improvement of saline-alkali land.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

The invention relates to the technical field of slope protection bricks, and discloses an engineering muck bionic slope protection brick and a mangrove forest root system synergistic reinforcement method thereof.The engineering muck bionic slope protection brick comprises a bionic slope protection brick body; the planting cavity is a cavity designed by simulating the root structure of a mangrove forest, and the planting cavity is formed in the bionic slope protection brick body and used for planting plant seeds or seedlings. The bionic slope protection brick body is obtained by taking engineering residue soil as a raw material, adding a certain proportion of adhesive, uniformly mixing and then pressing, and the planting cavity is formed in the bionic slope protection brick body by simulating the root structure of a mangrove forest, so that plant seeds or seedlings can be planted in the cavities of the main branches; and the roots of plant seedlings can grow downwards along the branch cavities and penetrate through the bionic slope protection brick body to enter the underground, so that the stability of the bionic slope protection brick body during slope protection is effectively improved.

Methods for stimulating plant growth and / or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and / or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

The invention belongs to the field of plant seed biology and rice breeding, and discloses an application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of storage resistance of rice seeds and a related breeding method. In particular discloses application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of the storability of rice seeds, the nucleotide sequence of the OsBTB05 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, and the amino acid sequence of the OsBTB05 protein is shown as SEQ ID NO: 3. The invention also discloses a molecular marker-assisted selection method for breeding the high-storage-resistance rice variety and a method for reducing the storage resistance of the rice seeds. The invention proves that the OsBTB05 gene is a key factor for regulating and controlling the storability of the rice seeds, and the expression level of the OsBTB05 gene is in significant positive correlation with the storability of the seeds. A clear molecular target and a brand-new gene resource are provided for improving the seed storage resistance from a genetic source.

The invention provides a degradable sodded strip for ecological restoration and a preparation method thereof, and belongs to the technical field of sodded strips, the degradable sodded strip comprises an upper-layer fiber web, a middle-layer seed and nutrient layer and a lower-layer grid which are sequentially laminated and compounded from top to bottom; the upper-layer fiber web is made of a hydrophilic degradable material and is used for absorbing moisture and preserving soil moisture; the middle seed and nutrient layer comprises plant seeds and a nutrient controlled release unit; the lower-layer grid is a three-dimensional grid which is made of a degradable high polymer material, has a three-dimensional communicated micropore structure and is used for mechanically guiding root growth; the nutrient controlled release unit is a microcapsule which takes a fertilizer as a core material and takes an environment response type polymer as a wall material, and the microcapsule is anchored in the fiber interweaving nodes of the lower layer grid through a cross-linking agent. According to the invention, environmental response type precise controlled release of plant rhizosphere nutrients and three-dimensional mechanical guidance of a root system growth path can be realized, and the utilization rate of the fertilizer and the survival rate and long-term stability of vegetation recovery under poor site conditions such as side slopes, river banks and mines are effectively improved.

The invention relates to a plant seed physical treatment device in the technical field of agriculture, in particular to a pretreatment device for rice seeds before sowing and a control method of the pretreatment device. The seed treatment device is composed of a water tank assembly, a water circulation and treatment mechanism, a ventilation air distribution mechanism, a seed containing frame, an intelligent control and power distribution mechanism, an overhead throw-in type ultrasonic treatment mechanism, a movable hoisting mechanism and the like. The device is high in intelligent program, simple and convenient to operate, capable of completing a series of pre-sowing pretreatment such as seed drying, seed selection, seed soaking, chest breaking and bud airing of rice seeds in the same box body, free of secondary carrying of the rice seeds, labor-saving, time-saving, water-saving and energy-saving, small in occupied area, low in cost and suitable for common small and medium-sized agricultural production collaborations.

The invention discloses a growth-promoting rhizosphere bacterium streptomyces cerevisiae QS9-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces setonii QS9-7 is obtained through separation, and the preservation number of the Streptomyces setonii QS9-7 is CGMCC (China General Microbiological Culture Collection Center) No.32329. Experiments show that the strain QS9-7 has a relatively strong inhibition effect on various plant pathogens, and shows excellent biocontrol bacterium potential. The strain can effectively improve radicle length, germ length, lateral root number and germination rate of plant seeds and plant height and stem diameter of plant seedlings, obviously promotes plant growth, and has high application value in green prevention and control of plant diseases.

The utility model relates to the technical field of greening side slopes, and discloses a road greening side slope which comprises a side slope, a plurality of protection boxes are vertically and horizontally arranged on the slope of the side slope, the upper end face and the bottom face of each protection box are of an opening structure, the bottom face of the lower end of each protection box is of a slope structure, the opening of the upper end of each protection box is vertically upward, and planting soil is laid in each protection box. Green plant seeds are planted in the planting soil, penetrating holes are formed in the four corners of the upper end face of the protection box, ground nails penetrate through the penetrating holes, and one ends of the ground nails penetrate into the slope; the protection box is installed on the side slope, the protection box is fixed through the ground nails, green plant seeds are planted in the planting soil in the protection box, the protection box can protect the seeds, the green plant seeds or green plant seedlings are prevented from being washed away by rainwater, then growth of green plants is guaranteed, the side slope is reinforced through roots of the green plants, and the green plants are protected. Planting of green plants is facilitated, and practicability is improved.

Agrochemical compositions including an agriculturally suitable carrier; one or more encysted or sporulated protozoa (protist); and an agricultural payload including an agrochemical with or without a food source for protozoa such as a agriculturally nonbeneficial bacteria are provided. Also provided is a modified plant seed, and methods for improving plant growth, reducing plant loss to pests, and controlling or eliminating pest infestation of a crop plant, each including use of the described agrochemical compositions.

The invention relates to an endophytic subspecies strain of salt-tolerant glutamic acid bacillus, the endophytic subspecies strain of the salt-tolerant glutamic acid bacillus is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC No: M20242321, and the 16S rDNA (ribosomal deoxyribonucleic acid) nucleotide sequence of the endophytic subspecies strain of the salt-tolerant glutamic acid bacillus is as shown in SEQ ID NO: 1. The strain can normally metabolize in a salt-free environment and a salt-containing environment, can tolerate the environment with the pH value of 6-11, has the effects of improving the germination rate of plant seeds and promoting plant growth in the salt-free environment and the salt-free environment, can improve the soil environment of the saline-alkali soil, is wide in application prospect, and provides a new biological resource for biological improvement of the saline-alkali soil.

Methods for stimulating plant growth and / or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and / or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

An ecological base material suitable for a high and steep rock slope, comprising: shale soil, a mixed improvement material, an adhesive, and a microbial agent at a mass ratio of (25-35): (42-75): (5-10): (3-5). The mixed improvement material comprises sludge-based biochar, straw, planting soil, polyacrylamide, and corn starch grafted acrylate. A repairing method using the ecological base material suitable for a high and steep rock slope, comprising mixing the prepared ecological base material and plant seeds and then spraying the mixture. The problem of difficulty in attaching ecological base materials and continuing slope recovery effects in existing high and steep rock slope repairing processes can be effectively solved.

The utility model belongs to the technical field of abandoned mine restoration, and particularly relates to an abandoned mine steep side slope slag pile horizontal anti-seepage and ecological reconstruction structure which comprises a reinforced concrete anti-seepage layer, a porous plant-growing concrete layer, a plant-growing bag layer and an active protective net which are sequentially arranged on the surface of a slag layer. The plant-growing bag layer is fixed on the slope surface through the active protective net and the anchor rod system, and plant seeds used for forming regreening vegetation on the surface of the plant-growing bag layer are arranged in the plant-growing bag layer. According to the utility model, a combined structure of the vegetation bag layer and the porous vegetation concrete layer is adopted, so that the growth space of the root system of the regreening vegetation is effectively extended, the water holding capacity of the plant growth matrix, the nutrition supply level of the plant and the wind and rain erosion resistance of the vegetation are remarkably improved, and the seepage-proofing and regreening effects are stable and lasting; meanwhile, the vegetation bag layer is stabilized through the active protective net and the anchor rod system, the stability of the structure is improved, and the structure is particularly suitable for seepage prevention and ecological reconstruction of steep slope slag piles.

The invention discloses an application of a TaSnRK2.8-5D gene and a related biological material thereof in regulating and controlling the protein content of plant seeds. The gene TaSnRK2.8-5D is cloned from wheat, the gene TaSnRK2.8-5D is overexpressed in wheat Fielder, a TaSnRK2.8-5D gene overexpressed plant is obtained, and a detection result shows that the protein content in wheat seeds can be remarkably increased by more than 1.31% through TaSnRK2.8-5D gene overexpression, so that the TaSnRK2.8-5D gene has an effect of increasing the protein content in the plant seeds, and the protein content in the wheat seeds can be increased by more than 1.31%. The method can be applied to plant breeding or quality improvement.

The invention discloses a seed coating containing an arbuscular mycorrhizal inoculant. The seed coating is prepared from the following components in parts by weight: 25 to 35 parts of the arbuscular mycorrhizal inoculant, 35 to 45 parts of a soil conditioner, 15 to 20 parts of talcum powder and 15 to 20 parts of polyvinyl acetate. The invention further provides a preparation method of the seed coating containing the arbuscular mycorrhizal inoculant and application of the seed coating to reduction of soil heavy metal pollution, increase of disease resistance and stress resistance of crops and promotion of crop growth. The seed coating containing the arbuscular mycorrhizal inoculant wraps the surfaces of plant seeds, provides good nutritional ingredients for growth and development of the seeds, and is beneficial to improving the germination rate of the seeds under heavy metal cadmium stress, reducing soil heavy metal cadmium pollution, improving soil quality, increasing soil fertility and improving disease resistance and stress resistance of crops; growth of seedlings is promoted, and crop quality and yield are improved.

The invention relates to the field of coast engineering and ecological restoration, and discloses vegetation concrete suitable for coast protection and a preparation method thereof.The concrete comprises cement, fine aggregate, coarse aggregate, a frozen plant growth matrix, water and an accelerator, and the matrix is composed of modified water-repellent clay, plant seeds, slow-release compound fertilizer, reinforced fibers and the like. The water resistance and the water retention capacity are remarkably improved through surface modification of the matrix, the slow-release compound fertilizer provides long-term nutrient support, and the matrix stability is improved through reinforced fibers; the interface bonding strength and the compression resistance of the concrete are optimized by adopting low-temperature mixing and an accelerator. The ecological protection system can adapt to the coastal tidal environment, and the ecological protection effects of high vegetation coverage rate and high durability are achieved.