134results about How to "Improve plant resistance" patented technology

A method of introducing an exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule into a seed is provided. The method comprises contacting the seed with the exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule under conditions which allow penetration of the exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule into the seed, thereby introducing the exogenous non-transcribable polynucleotide trigger, for example dsRNA, molecule into the seed.

The present invention relates to transgenic plants resistant to parasites that their normal life cycle includes feeding on the plant cytoplasm, including insects, nematodes and fungi, wherein the plants are engineered to produce small interfering RNAs (siRNAs) capable of silencing a parasite specific gene. Particularly the parasite gene is a stage-specific gene, more particularly a gene involved in essential, early developmental stages of the parasite in or on the plant.

The present invention provides compositions and methods for killing or suppressing growth of Gram-negative bacteria that infect, infest or cause disease in plants, including pathogenic, saprophytic and opportunistic microbes that cause disease in plants and food borne illness in people or in animal feed.

Compositions and methods for preventing fusarium head blight on target crop plants are disclosed.

The invention relates to a high-yield cultivation method for honeysuckles in low mountains and hills. The method includes the key technical steps such as variety selection, raising of seedlings by cutting, digging of planting holes, base fertilizer application and planting, covering of mulching films, pruning, integrated control of plant diseases and insect pests and topdressing. The method is characterized in that growth and development of plants of the honeysuckles are obviously promoted, the time limit ahead of the high-yield period is shortened, the yield of the honeysuckles is improved, and the quality of the honeysuckles is guaranteed. Compared with an ordinary planting method, the high-yield cultivation method enables the yield in the first year after transplant to be promoted by 56.7%, the yield in the second year after the transplant is promoted by 87.3%, and the yield in the third year after the transplant is promoted by 116.6%. However, no obvious changes happen on the content of chlorogenic acid of the honeysuckles and contents of galuteolin of the honeysuckles, the content of the chlorogenic acid of the honeysuckles and the content of the galuteolin of the honeysuckles all meet the standard provisions of <pharmacopoeia of the people's republic of China> (2010 version), and social benefits, economic benefits and ecological benefits are remarkable.

The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4,5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.

A disclosed nutrient solution for plants is prepared from the following raw materials in parts by weight: 20-30 parts of monoammonium phosphate, 10-15 parts of potassium nitrate, 8-16 parts of zinc phosphate, 4-8 parts of copper sulphate, 5-9 parts of ferrous sulphate, 3-7 parts of magnesium sulfate, 6-12 parts of ammonium molybdate, 20-25 parts of fresh pea juice, 15-20 parts of fresh aloe juice, 7-17 parts of fulvic acid, 6-14 parts of amino acids, 3-7 parts of gibberellin, 5-10 parts of abscisic acid, 9-16 parts of naphthylacetic acid, 11-17 parts of salicylic acid, 8-16 parts of protein hydrolysate, 10-18 parts of sodium silicate, 9-16 parts of cane sugar, 10-15 parts of thiourea and 15-30 parts of rose extracting liquid. The provided nutrient solution for plants is capable of promoting absorption of plants on a large amount of elements, substantially improving resistance of plants, improving output and quality, effectively reducing pesticide residue on plants and adjusting plantation structure of succeeding crops.

The invention relates to a bacillus subtilis for preventing and controlling Chinese herbal medicine soil-borne diseases and a preparation method of a bactericide thereof, in particular to a bacillus subtilis and a preparation method of the bactericide thereof. The bacillus subtilis X1 for controlling the Chinese herbal medicine soil-borne diseases is Bacillus with a preservation number of CGMCC No.4037 and a preservation date of 26th, July 2010. The bactericide is prepared by the following steps: inoculating an activated strain to a NYD fluid nutrient medium for enrichment culture to obtain a sterilized solution with 2-4 billion/millimetre in bacterial count concentration. The bacillus subtilis X1 of the invention is a bio-control bacterial strain which is provided with high control effect on the soil-borne diseases and the bactericide can enjoy a prevention rate of 60-80% on the Chinese herbal medicine soil-borne diseases including ginseng root rot, sclerotiniose, North China fritillary sclerotiniose, Schisandra chinensis root rot, seedling blight and the like. The bactericide bio-control preparation is safe and environment-friendly and does not damage drug resistance of nontarget organisms and inductive pathogenic bacteria.

Disclosed is a method of preventing diseases in plants which can bring about a redox reaction in the inside of plant cells thus inducing the expression of pathogen-resistance genes in the plant cells. There is no possibility that residual contents remain in soils. It is also possible to cultivate plants which are strong against diseases. By bringing water which contains reactive oxygen species and allows the reactive oxygen species to be held and to function for a long period into contact with plants, the expression of the pathogen-resistance genes which the plants possess is induced. Accordingly, the water containing reactive oxygen is absorbed into the plants to prevent diseases in plants.

Plants, plant cells, and seeds are described herein that grow well and are resistant to environmental stresses such as drought and insects, where the plants have one or more mutations that reduce or eliminate the interaction of MYC transcription factors with the JAZ proteins. The plants can have an additional mutation that reduces or eliminates the function of the PHYB gene, and/or a heterologous PIF4 transgene or PIF4 expression cassette to improve the growth of the myc mutant plants. Methods of making and using such plants, plant cells, and seeds are also described.

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter and terminator isolated from the maize lipid transfer protein 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

The present invention relates to Brassica plants comprising at least one mutant dwarfing allele of a DELLA protein encoding gene, nucleic acid sequences representing mutant DELLA dwarfing alleles, and mutant dwarfing DELLA proteins. The invention further relates to methods for generating and identifying said plants and alleles, which can be used to obtain plants with reduced height and increased lodging resistance.

This invention relates to crop plants of which the fruit dehiscence properties are modulated. More specifically the invention relates to improved methods and means for reducing seed shattering, or delaying seed shattering until after harvest, in plants, while maintaining at the same time an agronomically relevant threshability of the pods.

The invention relates to water-soluble fertilizer containing alginate and various nutrients and a preparation method of the water-soluble fertilizer. The water-soluble fertilizer comprises alginate, macro-elements N, P2O5 and K2O, medium elements Ca and Mg and trace elements Cu, Fe, Mn, Mo, Zn and B, wherein the weight percentage of the alginate is 3%., the total weight percentage of the macro-elements is 57%, the total weight percentage of the medium elements is 1%, and the total weight percentage of the trace elements is 0.64%. The preparation method includes: weighing the raw materials according to the weight percentage, and crushing and sieving; weighing the alginate, and evenly mixing with nitrogen fertilizer for standby; weighing the trace elements, and mixing with calcium and magnesium fertilizer for standby; weighing phosphorus fertilizer and potassium fertilizer, evenly mixing, adding the mixture, and sufficiently mixing to obtain the water-soluble fertilizer. By the water-soluble fertilizer, fruit yield can be increased by 20-30%, vegetable yield can be increased by 25-35%, and flower quality can be increased evidently.

The present invention provides compositions and methods for killing or suppressing growth of Gram-negative bacteria that infect, infest or cause disease in plants, including pathogenic, saprophytic and opportunistic microbes that cause disease in plants and food borne illness in people or in animal feed.

The present invention is related to the field of environmental protection, more specifically, to a foliage silicon fertilizer and a method for production of the fertilizer, which is a molybdenum-silica compound sol used for reducing heavy metal and nitrates in vegetables. The fertilizer, which is a molybdenum-silica compound sol, comprises 10-25 wt % silica, 0.05-5.5 wt % molybdenum ions. More preferably, the fertilize, which is a rare earth-molybdenum-silica compound sol, comprises 10-25 wt % silica, 0.05-5.5 wt % molybdenum ions and 0.1-7.5 wt % rare earth ions. By the combination of silica with molybdenum in the present invention, it is effective for preventing the absorption/accumulation of nitrates in vegetables. In addition, by the further combination with rare earth element, the prevention ability of the fertilizer from absorbing heavy metal and nitrates into vegetables is even enhanced. With a preparation method with normal pressure and relatively, low temperature, that is, with mild condition, simple process and high operability, large scale production of the present invention may be readily executed.

Rice OXHS4 gene controlling root growth and conferring enhanced drought resistance, and use thereof in genetic improvement of rice drought resistance is provided. OXHS4 gene is cloned using candidate gene screening method. Drought stress experiments at seedling stage and adult plant stage shows that overexpression of OXHS4 gene can improve drought resistance of transgenic rice, while the oxhs4 mutant exhibits sensitivity to drought, showing the function of said gene and the use thereof.

The invention relates to a soil conditioner for enhancing crop stress resistance, and preparation and use methods thereof, and belongs to the field of an agricultural soil conditioner. The soil conditioner for enhancing crop stress resistance is prepared from the following ingredients: 3.0 to 7.0 percent of fulvic acid, 0.20 to 1.0 percent of polyacrylamide, 0.2 to 1.0 percent of polyvinyl alcohol, 1.0 to 5.0 percent of manganese sulfate, 0.001 to 0.01 percent of proline, 1.0 to 5.0 percent of calcium lignosulphonate, 0.02 to 0.05 percent of boracic acid and the balance of water. The inventionalso discloses the preparation method and the use method of the soil conditioner. The soil conditioner for enhancing crop stress resistance, and the preparation and use methods have the advantages that the ingredients of the soil conditioner are simple; the cost is low; the price is cheap; the effects of maintaining the soil moisture, improving the soil agglomeration structure and enhancing the plant resistance can be achieved; meanwhile, the crop growth and development character can be improved; the crop yield is increased; the crop quality is improved; the crop benefits are high; high practicability is realized; the soil conditioner is suitable for being used under the drip irrigation condition; great popularization prospects are realized in the drought region. The preparation method and the use method are simple.

The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGPase. The subject invention also comprises a mutant plant small subunit of AGPase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

Transgenic plants, plant tissue, and propagation materials are disclosed that exhibit or convey increased resistance to pathogens of multiple higher-order phylogenetic lineages. The disclosed transgenic plants and plant tissues include plant cells containing a DNA construct encoding Gastrodia Anti-Fungal Protein (GAFP), also known as gastrodianin, an anti-fungal gene naturally occurring in a Chinese orchid, Gastrodia elata. Transgenic plants disclosed include herbaceous plants as well as woody plants, including fruit trees. Disclosed transgenic plants can also be beneficially utilized as rootstock, for instance rootstock for stone fruit crops such as peach, thereby conferring enhanced disease resistance to the rootstock without genetically altering the scion.

The invention relates to a plant growth activating and accelerating agent and its preparation method, comprising 1% to 30% of DA-6, 0.5% to 5% of high permeation agent, 1% to 10% of synergist, 1% to 12% of dispersant, 1% to 20% of emulsifier, and solvent. The plant growth activator and promoter is prepared by mixing high permeation agent, synergist, dispersant, emulsifier, and DA-6, stirring uniformly; adding the metal inorganic salt mixer into water, stirring to dissolve sufficiently; mixing said two mixers, and emulsifying. The plant growth activating and accelerating agent in the invention is provided with activation and toxicological harmless effect, which can considerably improve plant stress resistance and yield of plant.