The invention relates to a preparation method of a soil conditioner, belonging to the technical field of fertilizer preparation. The soil conditioner is mainly prepared from the following raw materials in parts by weight: 50-70 parts of plant straw carbon, 20-35 parts of fowl/livestock dung, 10-15 parts of bone meal, 1-8 parts of potassium sulfate, 2-8 parts of wood vinegar liquid, 3-9 parts of plant growth regulator and 0.5-1 part of active agent. The preparation method comprises the following steps: preparing carbon powder, pretreating the fowl/livestock dung, preparing a mixture, assaying and detecting, granulating and packaging. The soil conditioner is prepared by mixing the crop straw/bamboo/wood/grass carbon, which is used as the main component, with certain amounts of trace elements, plant growth regulator and bamboo/wood/grass vinegar liquid; since the soil conditioner not only is rich in the three main nutrient elements (nitrogen, phosphorus and potassium) which are essential for crops, but also contains secondary and trace elements, such as calcium, magnesium, sulfur, iron, boron, molybdenum, copper, cobalt and the like, the nutrient proportion of the soil conditioner is reasonable; and when being used as a conditioner, the soil conditioner can increase the organic content of soil and provide more comprehensive nutrients for crops, so that the crops have the advantages of good growth vigor, plump grains and much higher yield.