Bacterial strain used for producing ganoderma lucidum polysaccharides by complete feed liquid fermentation of rice bran and wheat bran

A technology of liquid fermentation, Ganoderma lucidum polysaccharide, applied in applications, fungal products, lichen products, etc., can solve the problems of low production efficiency, limited quantity of Ganoderma lucidum, unstable quality and yield, etc., to save costs and resources, good social benefits, The effect of good economic value

Inactive Publication Date: 2014-01-01
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the artificial cultivation of Ganoderma lucidum has a long cycle, low production efficiency, high labor intensity, and is subject to seasonal and environmental restrictions. It is vulnerable to pe

Method used

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  • Bacterial strain used for producing ganoderma lucidum polysaccharides by complete feed liquid fermentation of rice bran and wheat bran
  • Bacterial strain used for producing ganoderma lucidum polysaccharides by complete feed liquid fermentation of rice bran and wheat bran
  • Bacterial strain used for producing ganoderma lucidum polysaccharides by complete feed liquid fermentation of rice bran and wheat bran

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Effect test

Embodiment 1

[0049] Embodiment 1 Ganoderma lucidum ultraviolet-microwave mutagenesis

[0050] ① Preparation of spore suspension

[0051] Inoculate Ganoderma lucidum on a 9cm sterilized solid medium plate and culture it for 4 days, then wash it with 10mL sterile physiological saline, use a sterilized brush to brush the vegetative body back and forth, and collect the washing solution.

[0052] ②UV mutagenic effect curve and selection of UV mutagenic dose

[0053] Take 20 9cm plates, each containing 5 mL of newly prepared spore suspension. Every two as a group, divided into 10 groups. Irradiate for 0, 5, 10, 15, 20, 25, 30, 45, 60, 150s respectively (see attached figure 2 ), and then take 1mL of appropriate dilution for each dish and spread it on the plate. The plates were wrapped with black cloth and then cultured in a dark 28°C incubator with no light. Count the colonies after the plate grows out. For each group, the average number of colonies in two dishes was used as the number of ...

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Abstract

The invention belongs to the field of microbial application technology and food biotechnology, and discloses a ganoderma lucidum strain used for producing polysaccharides by complete feed liquid fermentation of rice bran and wheat bran. Ganoderma lucidum JSU6161314 is stored in China Center for Type Culture Collection (CCTCC) on 25th, June, 2013, preservation number is CCTCC M2013287, and the bacterial strain is named as Ganoderma lucidum CCTCC M2013287. According to a method disclosed in a drawing of the invention, the novel bacterial strain, which is capable of realizing high yield of ganoderma lucidum polysaccharides, is obtained by modifying ganoderma lucidum CFCC6043. The novel bacterial strain is used for rapid fermentation in a rice bran and wheat bran complete feed liquid culture medium and high yield of ganoderma lucidum polysaccharides. After fermentation, hypha dry weight and hypha polysaccharide yield increase 23.0% and 45% respectively compared with that of the original strain.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a new mutagenic strain of a Ganoderma lucidum strain suitable for growing in a composite liquid medium of rice bran and bran whole material and high-yielding Ganoderma lucidum polysaccharide. Background technique [0002] Since Chihara et al. (references: Chihara G, MaedaY, Hamuro J, et al. Inhibition of mouse sarcoma180by polysaccharides from Lentinus edodes (Berk.) sing [J]. Nature, 1969, 222 (5194): 687-688.) In 1969, he published a paper on "Nature" and revealed that lentinan has anti-tumor activity. More than 200 medicinal fungi with anti-tumor activity have been found, some of which are edible fungi, such as Grifola frondosa and Hericium erinaceus , Ganoderma lucidum, etc. The mycelium, fruiting bodies, sclerotia or spores of these edible fungi can produce various active ingredients such as amino acids, proteins, vitamins, polysaccharides, glycosides...

Claims

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Application Information

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IPC IPC(8): A01H15/00A01G1/04A01H1/06
Inventor 刘伟民郭天龙沈国栋张笑飞周文彪赵杰文马海乐
Owner JIANGSU UNIV
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