308results about "Lichen products" patented technology

The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.

The invention relates to a strain Pleurotus ostreatus TIB.BPE.10010 with the accession number being CGMCC No.6232. The invention also relates to a method of preparing L-erythrothioneine. The method comprises steps of culturing and fermenting the Pleurotus ostreatus CGMCC No.6232 to perform biosynthesis to form the L-erythrothioneine and extracting the L-erythrothioneine from mycelium cells.

The invention belongs to the technical field of microbe application and good bioscience and discloses a polysaccharide grifola strain produced through a rice bran and wheat bran complete feed liquid culture medium. grifola Grifola sp.JSU1301 is preserved in the China Typical Model Cultivation Center (CCTCC) in June 25th, 2013, and is numbered CCTCC NO: M 2013286 and named a grifola Grifola sp.CCTCC NO: M 2013286. According to the invention, a ultraviolet mutagenesis method is described with figures; a new strain capable of highly producing polysaccharide by starting with Grifola sp.CCTCC NO: M 2011113; the new strain quickly ferments and highly produces polysaccharide in the rice bran and wheat bran complete feed liquid culture medium; the mycelial dry weight and the mycelial polysaccharide productivity of a shake flask are increased by 16.8% and 8.57% compared with those of a starting strain, and those of fermentation in a tank are improved by 30.9% and 29.7%, which reach the highest level at present.

A substrate for mushroom cultivation comprises a polyene fungicide, in particular, natamycin. Mushrooms cultivated in such substrates can be harvested earlier than mushrooms cultivated in substrates which do not include polyene fungicides. Alternatively, mushrooms grown in a substrate comprising a polyene fungicide achieve a greater size than mushrooms grown for the same amount of time in a substrate which does not include a polyene fungicide.

The invention belongs to the field of microbial application technology and food biotechnology, and discloses a ganoderma lucidum strain used for producing polysaccharides by complete feed liquid fermentation of rice bran and wheat bran. Ganoderma lucidum JSU6161314 is stored in China Center for Type Culture Collection (CCTCC) on 25th, June, 2013, preservation number is CCTCC M2013287, and the bacterial strain is named as Ganoderma lucidum CCTCC M2013287. According to a method disclosed in a drawing of the invention, the novel bacterial strain, which is capable of realizing high yield of ganoderma lucidum polysaccharides, is obtained by modifying ganoderma lucidum CFCC6043. The novel bacterial strain is used for rapid fermentation in a rice bran and wheat bran complete feed liquid culture medium and high yield of ganoderma lucidum polysaccharides. After fermentation, hypha dry weight and hypha polysaccharide yield increase 23.0% and 45% respectively compared with that of the original strain.

The invention discloses a method for cultivating Poria cocos, belonging to the technique field of microbiological cultivating, which comprises: choosing good Poria cocos seeds by means of bacterial breeding, preserving and culturing the parent seeds, producing and breeding the initial species and cultivating species on the base of the former step; the parent species culturing comprising separating the parent species and culturing in the culture medium. By employing the method in this invention, bacterial filament and sclerotium can be produced, the culture medium is prepared artificially in the culture of parent species, original species and cultivating species course, and does not need natural pine and thus there is no need for trees cutting. The method is suitable for mass production and the planting cost is very low.

The invention describes a methodology for production of a secondary extra-particle fungal matrix for application as a mycological material, manufactured via a Type II actively aerated static packed-bed bioreactor. A pre-conditioned air stream is passed through a substrate of discrete elements inoculated with a filamentous fungus to form an isotropic inter-particle hyphal matrix between the discrete elements. Continued feeding of the air through the substrate of discrete elements and isotropic inter-particle hyphal matrixes develops an extra-particle hyphal matrix that extends from an isotropic inter-particle hyphal matrix in the direction of airflow into a void space within the vessel.

The invention provides a mutagenic strain of cordyceps militaris and a breeding method. A new high-yield strain of cordyceps militaris is obtained by chemical mutagenesis, the preservation number thereof is CGMCC NO. 2909, and compared with original starting strain, the dry weight of mycelium is increased by 103%, the content of adenosine is increased by 536%, the content of polysaccharide is increased by 22.2%, the content of protein is increased by 55.5%, and the content of mannitol is increased by 37.5 %. The new strain of cordyceps militaris does not degenerate after more than 20 times of subculturing, has genetic stability, and utilizes the research of random amplified polymorphic DNA (RAPD) to verify the difference on DNA level between the strain and the original starting strain. The invention also discloses the optimum process condition for extracting polysaccharide and adenosine of cordyceps militaris.

A culture method of a fruiting body of Antrodia camphorata is provided, which includes: (a) fermenting a culture medium containing yeast at 5-35° C. for 3-30 days; (b) adding wood flour to the fermented culture medium with stirring; (c) placing the wood flour and the culture medium into a vessel; (d) sterilizing the vessel containing the wood flour and the culture medium; (e) inoculating Antrodia camphorata strains into the sterilized vessel containing the wood flour and the culture medium, and culturing at 5-35° C., to form mycelia; (f) inoculating the wood flour containing Antrodia camphorata mycelia into a wood segment; (g) placing the wood segment inoculated with Antrodia camphorata mycelia in an environment where the temperature is 5-35° C. and the humidity is 65-85%, and culturing for a period of time; and (h) removing the wood flour remained on the wood segment, then placing the wood segment in an environment where the temperature is 15-35° C. and the humidity is 80-98%, and culturing for a period of time, to form a fruiting body of Antrodia camphorata. A culture method of Antrodia camphorata mycelia, a culture method of a fruiting body of Antrodia camphorate, and a fruiting body of Antrodia camphorata cultured by the same method are also provided. The fruiting body of Antrodia camphorata cultured by the method of the present invention is thick and solid, and the content of triterpenoids is comparable to that of wild Antrodia camphorate.

The invention discloses a fungus for promoting aquilaria plants to generate agilawood and application of the fungus. The fungus is Fomitopsis sp. ASAF01, and the preservation number in the China General Microbiological Culture Collection Center is CGMCC NO.7802. The Fomitopsis sp.ASAF01 can promote the heartwood formation of aquilaria sinensis after the Fomitopsis sp. ASAF01 is inoculated for six months, and the middle of the formed agilawood is not easy to rot. Furthermore, the strain can still grow at the temperature of 15-40 DEG C and pH value of 2-11 to indicate that the Fomitopsis sp.ASAF01 has strong environmental tolerance.

The invention relates to a protoplast mutation breeding method for improving the cordycepin content of cordyceps militaris. The method comprises the following steps of activated culture of bacterial strains, solid culture of mycelia, liquid mycelia culture, mycelia enzymolysis, protoplast nitrosoguanidine (NTG) mutation, protoplast regeneration culture, regenerated bacteria subculture, fermentation culture, filtering, further washing of mycelia, measurement of cordycepin content in the mycelia, preservation of the bacteria strain with high cordycepin content, measurement of the genetic stability and the like. Due to the adoption of the method, the cordycepin content of the cordyceps militaris can be increased.

The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.

The invention belongs to the technical field of edible mushrooms and particularly relates to a hypsizygus marmoreus Finc-B-3 strain and application thereof. The hypsizygus marmoreus strain provided by the invention is named as Finc-B-3 and is obtained by taking a strain TNN-12 and a strain F-FG as parents to be hybridized through systematic selection, belongs to hypsizygus marmoreus and is preserved in Institute of Microbiology Chinese Academy of Sciences, wherein the address is No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.11204 and the preservation date is 20150813. Compared with the prior art, the hypsizygus marmoreus Finc-B-3 strain provided by the invention has short culture and cultivation periods, so that the production efficiency of factories is greatly improved; the unit yield is high, the yields of bottles are similar, and topographic characteristics are good, so that sub-packaging and sales of products are very easy; and the Finc-B-3 has good comprehensive performances and a wide market prospect.

The invention relates to a new strain of edible fungus, belonging to the technical field of edible fungus development and utilization. The new grey variant strain of edible fungus with red pleurotus: Pleurotus djamor (Rumph.: Fr.)Boedijn, is the new strain of edible fungus selectively bred through single spore cross breeding and has the number No.1933 in CGMCC (China General Microbiological Culture Collection Center). The strain is characterized in that the color of sporophore is stable grey white, the whole sporophore is pure and transparent, the cellulose content is 5-12%, and the strain has favorable mouth feel and is used for producing the edible fungus in the high temperature season. Mycelium grows very fast and has strong ability in fighting bacteria and also has developed aerial hyphae. The hypha has clamp connection and the growth temperature of 3-45 DEG C; the temperature of sporophore differentiation is 18-35 DEG C, and the new grey variant strain is typical high temperature edible fungus. Straws, saw dust, bran, and the like are selected as cultivation materials. The edible fungus is cultivated in March to November. Cultivation materials produced in scale do not need high pressure or normal pressure sterilization, and the sporophore output is 15-25%. The strain is newly selected and bred edible fungus and has application value in the edible fungus field and ornamental fungus field.

High yield antibiotics producing fungus strain, preparation method and use thereof are provided. The fungus strain is a mutant derived from Glarea lozoyensis, and deposited in CGMCC with the accession number of CGMCC 2933. The preparation method concludes following steps: (a) mixing the culture media of Glarea lozoyensis strain ATCC 20957 with nitrosoguanidine, and obtaining mixture a; (b) mixing lywallzyme with the mixture a, and obtaining protoplasts; (c) regenerating the protoplasts, and obtaining single clones; and (d) culturing the single clones, then obtaining the mutant strain. This fungus strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable to be used in industry.

An aseptic culture method for pine nematode includes such steps as culturing Pseudomonas fluorescens GcM5-1A (CCTCC No.M204065), deactivating, culturing the calli of black pine, deactivating, preparing the aseptic ova of pine nematode, preparing the culture liquid from deactivated cells and calli and aseptic water, adding said aseptic ova, and culturing at 15-40 deg.C for 4-20 days.

Provided are a method of culturing Agaricus bisporus mycelium, the method comprising culturing Agaricus bisporus mycelium or spores thereof in a liquid medium comprising sugar cane extract, and a medium for culturing the Agaricus bisporus mycelium.

The present invention relates to artificial poria cultivating method. The wood cultivation process of poria features that it is realized through the following steps: selecting pine wood section, digging field, setting wood section into cellar, culturing wood primer, making wood primer, inoculating wood primer, transmitting wood primer, and separating mother seed. The present invention can cultivate robust poria in high yield and simple operation.

A hybrid mushroom culture of Agaricus bisporus, designated as strain B14528, includes a representative culture of the strain, which has been deposited under NRRL Accession No. 50900. A method of producing a hybrid mushroom culture of Agaricus bisporus comprising: mating a homokaryotic line J12998-s39 with a homokaryotic line BW-s191. Additionally, mushrooms, parts of the culture and products incorporating the culture are provided.

A novel soybean cultivar, designated SW90702, is disclosed. The invention relates to the seeds of soybean cultivar SW90702, to the plants of soybean SW90702 and to methods for producing a soybean plant produced by crossing the cultivar SW90702 with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar SW90702 with another soybean cultivar.