Protoplast mutation breeding method for improving cordycepin content of cordyceps militaris

Abstract

The invention relates to a protoplast mutation breeding method for improving the cordycepin content of cordyceps militaris. The method comprises the following steps of activated culture of bacterial strains, solid culture of mycelia, liquid mycelia culture, mycelia enzymolysis, protoplast nitrosoguanidine (NTG) mutation, protoplast regeneration culture, regenerated bacteria subculture, fermentation culture, filtering, further washing of mycelia, measurement of cordycepin content in the mycelia, preservation of the bacteria strain with high cordycepin content, measurement of the genetic stability and the like. Due to the adoption of the method, the cordycepin content of the cordyceps militaris can be increased.

Description

technical field [0001] The invention belongs to the technical field of mutagenesis and breeding of microbial strains, in particular to a method for protoplast nitrosoguanidine mutagenesis and breeding of high-yield cordycepin strains, according to which the strains with high yield of cordycepin can be bred and obtained. Background technique [0002] Cordyceps militaris ( Cordyceps militaris ), has another name called militaris, Cordyceps militaris, Cordyceps militaris, is Ascomycotina, Sclerotinia, Ergotomycetes, Ergotaceae, Cordyceps fungus, is Paecilomyces militaris ( Peacilomyces militaris ), its efficacy is similar to that of Cordyceps sinensis, and it has been proved to be very similar to Cordyceps sinensis in medicinal chemistry, pharmacology and clinical trials, and has good practical value in functional food, medicine and biological technology. Cordyceps militaris has been approved to enter the market by the Ministry of Health and the State Food and Drug Administr...

AI Technical Summary

Problems solved by technology

Mutation breeding has now become one of the commonly used breeding methods to increase the content of cordycepin militaris. Conventional mutation breeding mostly uses physical mutagenesis such as ultraviolet rays or chemical mutagens such as diethyl sulfate, lithium chloride, etc. Silk or spores are subjected to mutagenesis treatment, because this kind of mutagenesis method is difficult to obtain ideal mutagenesis effects due to the poor sensitivity of spores or mycelium to mutagen, difficulty in separating single spores, and long time-consuming breeding