2results about How to "Increase mutagenic effect" patented technology

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

The utility model discloses a plant seed mutagen treatment barrel, and relates to the technical field of agricultural auxiliary equipment. The cleaning device comprises a box body and an outer cylinder fixedly installed on one side of the front end in the box body, an inner cylinder is rotationally connected into the outer cylinder, a stirring assembly is arranged in the inner cylinder, and a cleaning assembly is arranged at the bottom of the end face of the rear end of the outer cylinder. Plant seeds in the inner cylinder can be stirred through the stirring assembly, so that all the plant seeds can be completely soaked in the medicament for a certain time, the situation that the plant seeds are not completely soaked is avoided, the mutagenesis effect of the plant seeds is improved, meanwhile, the water inlet pipe can be prevented from being damaged, and the service life of the plant seeds is prolonged. The inner wall of the outer cylinder and the outer wall of the inner cylinder are cleaned through the cleaning assembly, the cleaning efficiency is high, the cleaning effect is good, the workload is reduced, and meanwhile the follow-up plant seed mutagenesis operation is prevented from being affected.