Mutagenesis method for microorganism in supercritical CO2 solvent

Abstract

The invention provides a microorganism induction mutation method in a supercritical CO2 dissolving agent. The microorganism induction mutation method adopts supercritical CO2 as the dissolving agent, and organic compound comes into contact with microorganism in the supercritical CO2, to generate induction mutation to the microorganism. All selected organisms has no carcinogenesis, teratogennicity and mutagenic action to human bodies, therefore, the safety is very high. CO2 can bring the organism to a microorganism cell in supercritical CO2 medium, and the organic compound and a genetic substance in the microorganism cell play the effect on the induced mutation of the microorganism. The supercritical CO2 adopts a green dissolving agent with high safety, the organism used for induced mutation has no carcinogenesis, teratogennicity and mutagenic action to human bodies, therefore, compared with the prior microorganism induction mutation technology, the microorganism induction mutation method has the prominent advantages that induction mutation reagent has high safety to human bodies, has no carcinogenesis, teratogennicity and mutagenic action, and has no effect to environment, and the induction mutation effect is relatively ideal.

Description

(1) Technical field [0001] The present invention relates to a kind of microbial mutagenesis method, especially a kind of supercritical CO 2 Methods of microbial mutagenesis in solvents. (2) Background technology [0002] Microbial mutagenesis is a very commonly used industrial biotechnology. The current mutagenesis methods are mainly divided into chemical methods and physical methods. The chemical method is to use chemical mutagens, such as nitroguanidine, diethyl sulfate, etc. to mutate microorganisms in water to improve the performance of microorganisms, especially to increase the expression of the desired target product. Physical mutagenesis is to mutate microorganisms by physical means, such as ultraviolet irradiation and γ-ray irradiation, and to screen strains with improved performance from the mutated microorganisms. At present, the chemical mutagens that are more effective for microbial mutagenesis have carcinogenic and teratogenic effects on the human body, have v...

AI Technical Summary

Problems solved by technology

At present, the chemical mutagens that are more effective for microbial mutagenesis have carcinogenic and teratogenic effects on the human body, have very poor safety, and cause serious harm to the environment after use.

Among the methods of physical mutagenesis, the method with better mutagenesis effect also has carcinogenic and teratogenic effects on the human body, and the mutagenesis process has a serious impact on the environment. poor