24results about How to "Good genetic stability" patented technology

The invention belongs to the technical field of plant cultivation, provides application of bacillus H8 in cultivation of emilia sonchifolia, and particularly relates to bacillus H8, a bacillus H8 suspension with the concentration of 102-104 CFU / mL is prepared and applied to the position 2-3 cm away from the root of emilia sonchifolia as a bio-fertilizer once every 7 days, plant growth can be remarkably promoted, and the yield of emilia sonchifolia is increased. The content of medicinal components of chlorogenic acid and quercitrin in the leaves is increased; the method solves the problems of soil degradation, unstable medicinal material quality and the like caused by dependence on chemical fertilizers and pesticides in traditional cultivation, is suitable for green and efficient cultivation of emilia sonchifolia, and has the advantages of simplicity and convenience in operation, environment friendliness, low cost and the like.

The invention relates to the technical field of strain breeding, in particular to a high-yield beta-glucan Meyrowia guilliermondii strain and application thereof. According to the present invention, the high-yield beta-glucan mutant strain obtained through ARTP mutagenesis has the name of Meyerozyma guilliermondii NCUASD7, has been preserved in Guangdong Microbial Culture Collection Center on November 28, 2025, and has the preservation number of CDMCC NO: 67365; aRTP mutagenesis is high in mutation rate, good in hereditary stability and safer to operate, a large number of mutation libraries can be obtained within a short time, high-throughput primary screening and shake flask secondary screening are conducted through a 24-deep-hole plate, a yeast mutant strain with the high beta-glucan yield capacity is obtained, then subculture is conducted on the high-yield mutant strain, the hereditary stability of the strain is verified, and the high beta-glucan yield is obtained. The mutant strain not only has the characteristic of high yield of beta-glucan, but also can maintain good genetic stability.

This invention discloses a high-mannitol-producing strain of *Limosilactobacillus fermentum* and its applications, belonging to the field of biosynthesis technology. This strain, named *Limosilactobacillus fermentum* PY20 (GDMCC NO: 67749), was obtained from naturally squeezed persimmon juice. It exhibits excellent mannitol synthesis ability in fructose-based fermentation, growing and converting mannitol in a medium containing 30–60 g / L fructose with a conversion rate exceeding 69.77%. This invention overexpresses the endogenous mannitol dehydrogenase gene of PY20 in this bacterium. The resulting engineered bacterium grows and converts mannitol in a medium containing 30–60 g / L fructose with an efficiency exceeding 78.13%, representing an increase of over 11% in both mannitol yield and conversion efficiency compared to PY20, and demonstrating good genetic stability.

This invention discloses a drug-resistant Ewing sarcoma cell line, named human Ewing sarcoma cell ES 003, which was deposited at the China Center for Type Culture Collection on March 10, 2026, with accession number CCTCC NO: C202642. The cell line exhibits doxorubicin resistance; specifically, doxorubicin has an IC50 of [missing information - likely referring to an IC50 value] against the human Ewing sarcoma cell ES 003. 50 Above 5μM.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

The invention provides a strain for producing L-isoleucine and a construction method and application of the strain. Pck genes of the strain are knocked out, ilvBN (G20D, I21D and I22F), ilvA (V140M, F383A), lysC (T311I), hom (G378I), thrB (A20G), ppc, aspB, thrC, asd and brnFE are over-expressed, and the strain is prepared through operons Psod-ilvBN (G20D, I21D and I22F)-ilvA (V140M, F383A)-Ptuf-thrA-thrB-thrC and Psod-ppc-aspB-Ptuf

This invention relates to the fields of genetic engineering and microbial lipid metabolism regulation, specifically to a method for increasing the pentadecanoic acid content in *Schizochytrium*. The method includes constructing a gene knockout strain, wherein the ScAMPKα1, ScAMPKα2, or ScAMPKα3 genes are individually knocked out in *Schizochytrium* using homologous recombination technology. The amino acid sequence of the protein encoded by the ScAMPKα1 gene is shown in SEQ ID NO.1, the amino acid sequence of the protein encoded by the ScAMPKα2 gene is shown in SEQ ID NO.2, and the amino acid sequence of the protein encoded by the ScAMPKα3 gene is shown in SEQ ID NO.3. This invention is the first to knock out the adenosine monophosphate-activated protein kinase AMPKα subunit gene, effectively increasing the accumulation level of pentadecanoic acid in *Schizochytrium*.

The invention belongs to the field of microbial fermentation engineering, and particularly relates to a microbial strain with high yield of pyripyropene A and application thereof. The strain QLN-015 is preserved in the China General Microbiological Culture Collection Center on February 2, 2026, the preservation number is CGMCC No.42501, the classification name is Penicillium coprosum, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The pyripyropene A prepared by the invention is a precursor compound of afidopyropen, belongs to a novel insecticide with a novel structure, high efficiency and low toxicity, and shows remarkable market application prospect and industrial development potential.

This invention belongs to the field of genetic engineering technology and discloses a method for synthesizing mussel adhesive protein using gene recombination technology, as well as related recombinant vectors and strains. The method uses the core functional genes of mussel byssalin 3 and 5 as templates, and constructs a recombinant expression vector pET-20b-fp-cut through artificial synthesis and overlapping PCR gene tandem. This vector is then transformed into *E. coli* BL21 to obtain the recombinant strain BL21 / pET-20b-fp-cut. A gradient-scale fermentation process is used to optimize the efficient expression of the target protein. The product is then extracted using a salting-out-acetic acid extraction combined with tyrosinase-catalyzed purification process. Finally, multi-dimensional quality testing is used to verify the indicators. This invention also provides the aforementioned recombinant expression vector and recombinant strain. This method can realize the industrial production of mussel adhesive protein, with a fermentation unit of 5.2 g / L, a dopa content of 11.2%, and a purity of 96.5%. It also exhibits low immunogenicity and meets quality standards, showing broad application prospects in biomedicine, cosmetics, and other fields.

The invention discloses an agrobacterium tumefaciens-mediated bottle gourd stable genetic transformation method. The invention provides a bottle gourd stable genetic transformation system mediated by agrobacterium tumefaciens. The system provides a key technical platform for gene function research and molecular breeding of bottle gourds. An agrobacterium tumefaciens-mediated genetic transformation method is adopted, and the principle is that a target gene is inserted into a modified T-DNA region, an agrobacterium tumefaciens infection mechanism is utilized to realize transfer and integration of an exogenous gene to a plant cell, and a transgenic plant is obtained by combining a tissue culture technology. According to the method, screening culture is not carried out, after co-culture is carried out for two days, a fluorescence microscope is used for observing the fluorescence area of a groove part of a seed notch, the agrobacterium infection efficiency is judged, screening is carried out, and detection is carried out again after seedlings are obtained, so that the time is saved by 30-40 days compared with genetic transformation of plants needing screening culture; the method has the advantages of good hereditary stability and simple operation.

This invention discloses a Hunan pig SNP molecular marker and its application. The Hunan pig SNP molecular marker of this invention includes at least one of 64,055 SNP molecular markers. The genomic positions of these 64,055 SNP molecular markers were determined by sequence alignment based on the pig reference genome Sscrofa11.1. The Hunan pig SNP molecular markers of this invention are derived from the diverse local pig breeds of Hunan, exhibiting broad coverage, good site specificity, strong universality, and high accuracy. They can effectively distinguish local pig breeds from different origins and provide important technical support for the molecular breeding application of Hunan pigs.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

The application discloses a tylosin A high-yield engineering strain and application thereof, and belongs to the field of genetic engineering and fermentation technology. The strain is obtained by integrating a carbon mycose synthesis gene module (M14) overexpression cassette driven by a constitutive strong promoter into a genome and knocking out a negative regulation gene tylQ , so that the yield is greatly improved. Experiments show that the yield of the engineering strain in FFM culture medium reaches 1183 mg / L, which is 10 times that of a wild type; the yield can reach 1491.5 mg / L after adding alpha-ketoglutaric acid, and the genetic stability is good. The 5L fed-batch fermentation titer reaches 5.63 g / L. The application provides an efficient and stable industrial strain for large-scale production of tylosin A.

The invention discloses an aspergillus niger genetically engineered bacterium with high amylase and glucoamylase activity and a construction method and application thereof. The regulation effect of the hog1 gene on synthesis of aspergillus niger amylase and glucoamylase is found for the first time, and the constructed aspergillus niger genetically engineered bacterium is obtained by knocking out the aspergillus niger hog1 gene through a gene editing technology. The amylase producing activity of the genetically engineered bacterium is improved by 2.2 times compared with that of a wild strain, the glucoamylase producing activity is improved by 8.5 times, synchronous improvement of the two enzyme activities is achieved, the genetic stability is good, and the problem that in the prior art, the aspergillus niger amylase activity and the glucoamylase activity are insufficient is solved. The aspergillus niger genetically engineered bacterium provided by the invention has high amylase and glucoamylase activity, can significantly improve the production efficiency of enzyme preparations and reduce the industrial cost, and has wide application prospects in the fields of starch processing, biofuel and the like.

The invention discloses an application of a rape NLR type gene cluster in enhancing rice resistance. The rape NLR protein pair consists of the following proteins (1) and (2): (1) BnNLR1.1: an amino acid sequence is shown as a sequence 1 in a sequence table; and 2) BnNLR1.2: the amino acid sequence is shown as a sequence 3 in the sequence table. When the rape NLR protein pair is adopted to construct a double-gene heterologous transformation system, the innate immune response of rice can be effectively stimulated, so that the disease resistance of the rice is enhanced. The method has good hereditary stability and disease-resistant effect, provides a new technical path for cultivating a new variety of high-yield, high-quality and disease-resistant rice, and has important agricultural application value and popularization prospect.

The present application relates to a kind of monocotyledon green wisteria rapid propagation method, using the characteristics of green wisteria specific tissue cell totipotency, under open condition, using liquid medium to induce green wisteria specific site of in vitro leaf split differentiation, produce regenerative root and regenerative bud, finally form plant.The present application aims at the rapid propagation method of green wisteria leaf, without sterile environment, convenient to take material, easy to operate, low in cost, short in period, high in survival rate, with the advantages of high efficiency, economy, easy to scale, factory, etc., in green wisteria rapid seedling, specific material preservation and propagation has greater application value.

The invention discloses construction and application of escherichia coli for producing L-valine, and belongs to the technical field of biological engineering. According to the escherichia coli capable of producing the L-valine, the escherichia coli capable of producing the L-valine is obtained through rational transformation of metabolic engineering, the L-valine yield can reach 95.0 g / L or above when the escherichia coli is fermented in a 5L fermentation tank for 40 h, the glucose conversion rate reaches 50% or above, and the escherichia coli has good genetic stability and is suitable for industrial production. The mdh promoter with weakened expression intensity is also screened and identified, and the concentration of the byproduct succinic acid in the fermentation liquor can be effectively reduced.

The application belongs to the technical field of biological medicine, and provides a mouse anti-human PRCC monoclonal paired antibody, a hybridoma cell strain and application thereof. The application provides hybridoma cell strains 5F9C2 (CCTCC NO: C2025312) and 15F7B5 (CCTCC NO: C2025341) which respectively secrete antibodies with unique heavy chain / light chain variable regions; the antibodies can be used for immunohistochemical and immunoblotting detection of PRCC alone, and can also be paired to establish a sandwich method system through an enzyme-linked immunosorbent assay. The antibodies provided by the application are high in specificity and sensitivity, and can be used for accurate qualitative and quantitative analysis of PRCC.

The present application relates to the technical field of rotavirus, and particularly relates to a G8P[8] type group A rotavirus CZ-23 and application thereof.A G8P[8] type group A rotavirus CZ-23 has been preserved in the China General Microbiological Culture Collection Center CGMCC on April 11, 2023, the address of the preservation center is No. 1, Xibaheyanli 3, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 45559.The present application provides a new human rotavirus wild type strain, which is first reported to be isolated in China, the virus replication capacity of the strain is strong, the genetic stability is good, the strain can be applied to develop a vaccine strain of human rotavirus vaccine, and is suitable for a live attenuated rotavirus oral vaccine.

The application relates to the field of biotechnology, in particular to a recombinant Lactococcus lactis strain and a preparation method thereof. The recombinant Lactococcus lactis strain provided by the application comprises an expression cassette and an expression plasmid, the expression cassette is an expression cassette integrated into the chromosome of the Lactococcus lactis, and comprises a first promoter and a T7 RNA polymerase gene; the expression plasmid comprises a T7 promoter and an exogenous protein gene. The recombinant Lactococcus lactis strain has high transcription intensity, can significantly improve the yield of the exogenous protein, has good genetic stability, does not express an antibiotic-resistant protein, and has high safety.

This invention relates to a nucleic acid construct for an insect cell-baculovirus expression system and its applications, belonging to the field of genetic engineering. This invention provides a novel nucleic acid construct, a corresponding insect cell-baculovirus expression system, and its applications. Specifically, this invention constructs a chimeric promoter capable of efficiently driving the transcription of exogenous genes by creatively combining specific enhancers and promoters and designing their sequence and orientation. Furthermore, it combines independent... dormouse The promoter is used to construct a dual promoter system, which forms two independent transcription units. This dual promoter system can be placed in the same vector to efficiently drive the expression of the same target gene at different stages of baculovirus infection of insect cells, greatly enhancing the expression intensity and persistence of the target gene at the transcriptional level, and ultimately increasing the yield of the target protein.

The invention relates to the field of animal health. The present invention provides an attenuated infectious bronchitis virus (IBV). The attenuated infectious bronchitis virus can protect chickens from IBV infection.

The present application relates to the field of microbial breeding and biofermentation engineering, and discloses a NTG-ARTP compound mutagenesis Aureobasidium pullulans mutant strain, a mutagenesis method and application thereof, and a method for producing eicosapentaenoic acid. The preservation number of the mutant strain is CCTCC NO: M 20253077. The NTG-ARTP compound mutagenesis Aureobasidium pullulans mutant strain provided by the present application with the preservation number of CCTCC NO: M 20253077 can realize the synergistic high production of EPA and DHA, and has excellent genetic stability. The mutant strain is suitable for a conventional industrial fermentation system, and can further improve the production capacity of EPA and DHA by combining a time sequence plant hormone induction strategy, thereby providing a high-quality strain support for large-scale production of high-purity EPA.