9results about How to "Good genetic stability" patented technology

This invention discloses a high-mannitol-producing strain of *Limosilactobacillus fermentum* and its applications, belonging to the field of biosynthesis technology. This strain, named *Limosilactobacillus fermentum* PY20 (GDMCC NO: 67749), was obtained from naturally squeezed persimmon juice. It exhibits excellent mannitol synthesis ability in fructose-based fermentation, growing and converting mannitol in a medium containing 30–60 g / L fructose with a conversion rate exceeding 69.77%. This invention overexpresses the endogenous mannitol dehydrogenase gene of PY20 in this bacterium. The resulting engineered bacterium grows and converts mannitol in a medium containing 30–60 g / L fructose with an efficiency exceeding 78.13%, representing an increase of over 11% in both mannitol yield and conversion efficiency compared to PY20, and demonstrating good genetic stability.

This invention discloses a drug-resistant Ewing sarcoma cell line, named human Ewing sarcoma cell ES 003, which was deposited at the China Center for Type Culture Collection on March 10, 2026, with accession number CCTCC NO: C202642. The cell line exhibits doxorubicin resistance; specifically, doxorubicin has an IC50 of [missing information - likely referring to an IC50 value] against the human Ewing sarcoma cell ES 003. 50 Above 5μM.

The application discloses a method for constructing a Vietnamese terminalia regenerative system, and comprises the following steps: Vietnamese terminalia stem segment test materials are obtained by grafting seedling saplings with superior branches, semi-lignified stem segments with axillary buds are used as explants, and after disinfection, the explants are inoculated into an induction culture medium to induce axillary buds, then the induced axillary buds are subjected to micro-branch cutting to carry out proliferation and rooting culture, and a large number of Vietnamese terminalia regenerative plants can be obtained. The method has the advantages of high propagation efficiency, high phenotype consistency, good genetic stability and the like, greatly shortens the production cycle of Vietnamese terminalia seedlings, reduces the variation risk of the seedlings, and provides high-quality seedlings for ecological afforestation and landscaping.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

This invention provides a uracil-producing strain, its construction method, and its application. The strain utilizes CRIPSR / Cas9 gene editing technology to produce... E. coli On the UR14 genome, firstly by knocking out psuG Gene, black Gene, rutA Genes and upp Genes were introduced to block the breakdown of uracil; then, the uridine phosphorylase gene was overexpressed. udp and pyrimidine-5'-nucleotide nuclease gene ppnN This synergistically enhances the synthesis and accumulation of uracil; finally, overexpression of the phosphoribosyltransferase gene... pgm, Further enhancing the conversion of the byproduct 1-phosphate ribose into the precursor 5-phosphate ribose-1-pyrophosphate improves carbon utilization. The resulting strain exhibits good genetic stability and high fermentation yield, enabling stable production of uracil and demonstrating broad application prospects.

The present application relates to a kind of monocotyledon green wisteria rapid propagation method, using the characteristics of green wisteria specific tissue cell totipotency, under open condition, using liquid medium to induce green wisteria specific site of in vitro leaf split differentiation, produce regenerative root and regenerative bud, finally form plant.The present application aims at the rapid propagation method of green wisteria leaf, without sterile environment, convenient to take material, easy to operate, low in cost, short in period, high in survival rate, with the advantages of high efficiency, economy, easy to scale, factory, etc., in green wisteria rapid seedling, specific material preservation and propagation has greater application value.

The application belongs to the technical field of biological medicine, and provides a mouse anti-human PRCC monoclonal paired antibody, a hybridoma cell strain and application thereof. The application provides hybridoma cell strains 5F9C2 (CCTCC NO: C2025312) and 15F7B5 (CCTCC NO: C2025341) which respectively secrete antibodies with unique heavy chain / light chain variable regions; the antibodies can be used for immunohistochemical and immunoblotting detection of PRCC alone, and can also be paired to establish a sandwich method system through an enzyme-linked immunosorbent assay. The antibodies provided by the application are high in specificity and sensitivity, and can be used for accurate qualitative and quantitative analysis of PRCC.

The present application relates to the technical field of rotavirus, and particularly relates to a G8P[8] type group A rotavirus CZ-23 and application thereof.A G8P[8] type group A rotavirus CZ-23 has been preserved in the China General Microbiological Culture Collection Center CGMCC on April 11, 2023, the address of the preservation center is No. 1, Xibaheyanli 3, Chaoyang District, Beijing, and the preservation number is CGMCC NO. 45559.The present application provides a new human rotavirus wild type strain, which is first reported to be isolated in China, the virus replication capacity of the strain is strong, the genetic stability is good, the strain can be applied to develop a vaccine strain of human rotavirus vaccine, and is suitable for a live attenuated rotavirus oral vaccine.

The present application relates to the field of microbial breeding and biofermentation engineering, and discloses a NTG-ARTP compound mutagenesis Aureobasidium pullulans mutant strain, a mutagenesis method and application thereof, and a method for producing eicosapentaenoic acid. The preservation number of the mutant strain is CCTCC NO: M 20253077. The NTG-ARTP compound mutagenesis Aureobasidium pullulans mutant strain provided by the present application with the preservation number of CCTCC NO: M 20253077 can realize the synergistic high production of EPA and DHA, and has excellent genetic stability. The mutant strain is suitable for a conventional industrial fermentation system, and can further improve the production capacity of EPA and DHA by combining a time sequence plant hormone induction strategy, thereby providing a high-quality strain support for large-scale production of high-purity EPA.