Propagation method of Chinese yam exsomatize

A technology of in vitro propagation and yam, which is applied in the field of reproduction, can solve the problems of tuber germination into seedlings and application, which have not been further studied and occupy a large space.

Inactive Publication Date: 2010-02-03
HENAN NORMAL UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

The first two propagation methods have high genetic stability, but they take up a lot of space. The third method currently only has the preliminary induction of micro-tubers, and there is no further research on the germination and application of tubers.

Method used

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Effect test

Embodiment Construction

[0004] The present invention has following embodiment:

[0005] 1. Induced formation of Chinese yam-like protocorms

[0006] On the ultra-clean workbench, cut the test-tube seedlings of Chinese yam into about 1cm long stems with axillary buds and inoculate them on the medium of MS+TDZ 0.1~6mg / L+KT 1~4mg / L, and carry out under the conditions of the culture room Culture, 30 ~ 40d can induce the formation of protocorm-like.

[0007] 2. Proliferation of Chinese yam class protocorms

[0008] On the ultra-clean workbench, cut the induced Chinese yam protocorm into small pieces with a diameter of about 0.5cm and inoculate it on MS+TDZ 1~4mg / L+KT 1~4mg / L medium under sterile conditions , under the conditions of the culture room, the protocorm-like can proliferate in large quantities.

[0009] 3. Differentiation of Chinese yam protocorms

[0010] On the ultra-clean workbench, transfer the protocorm-like stems induced or subcultured to MS+2,4-D 0.05~0.1mg / L+6-BA 1~2mg / L+KT 2~4mg / L ...

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Abstract

The invention discloses a propagation method of Chinese yam exsomatize, aiming at providing a propagation method of Chinese yam exsomatize with good genetic stability, less propagation usage space, and convenient storage and transportation. The technical scheme of the invention comprises the following steps of: (1) inducing the formation of Chinese yam protocorm; (2) propagating the Chinese yam protocorm; (3) differencing the Chinese yam protocorm; (4) rooting regeneration plant of the Chinese yam protocorm; and (5) hardening and transplanting the regeneration plant of the Chinese yam protocorm. The method is used for the propagation of the Chinese yam.

Description

Technical field: [0001] The invention relates to a propagation method, in particular to a yam in vitro propagation method. Background technique: [0002] Yam has both edible value and medicinal value. Huai yam is famous all over the world for its unique taste, nutritional value and medicinal effect due to its unique origin and variety. However, the traditional tuber propagation method can not meet the production needs. Extensive research has been done on its in vitro propagation. At present, in the research of Huai Yam, it is believed that Huai Yam can be propagated in vitro through the ways of axillary branching, polybud induction and microtuber induction. Among them, the axillary branch is to cut the stem section with axillary buds in the medium, and the axillary buds will differentiate into seedlings, and the base will differentiate into roots to form a complete plant; the polybud induction type is to remove the apical dominance by adjusting the hormone concentration in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李明军赵喜亭邓丽张晓丽李海兵赵娇陈明霞
Owner HENAN NORMAL UNIV
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