32 results about "Interspecific hybridization" patented technology

The invention discloses a molecular marker closely linked with a thin-skinned muskmelon bacterial fruit blotch resistance gene and application thereof, and belongs to the field of muskmelon disease resistance identification. According to the method, a disease-resistant muskmelon strain and a susceptible muskmelon strain are subjected to interspecific hybridization, genetic analysis is carried out on BFB-resistant characters of muskmelons by utilizing an F2 group, a high-resistance gene pool and a high-susceptibility gene pool are constructed on the basis, and resistance candidate genes of the muskmelons are mined by utilizing a BSA-seq technology and a molecular marker technology. Research results can provide a research basis for disease resistance character gene mining and mechanism research, and the method has important scientific significance for screening and application of muskmelon disease-resistant germplasm resources. The molecular marker KASP988272 is developed according to the genotype difference between candidate gene parents, and the genotype identification accuracy of the molecular marker in an F2 group is as high as 80%. The developed molecular marker closely linked with the bacterial fruit blotch resistant gene is used for rapidly and efficiently identifying the bacterial fruit blotch resistant thin-skinned muskmelon variety, and a technical support is provided for selection of the bacterial fruit blotch resistant thin-skinned muskmelon variety.

The application provides a method for creating a strawberry germplasm for controlling weeds and application thereof, and belongs to the technical field of biological breeding. Forest strawberry 'Ruegen' is used as a female parent, and yellow hair strawberry is used as a male parent, F1 generation is obtained by hybridization, F2 generation is obtained by screening selfing of the F1 generation, and the F2 generation is screened to obtain a strain with less flowering, no fruiting, easy to sprout stolon and easy to tiller, which is the strawberry for controlling weeds. The forest strawberry 'Ruegen' with strong resistance and not sprouting stolon is crossbred with the yellow hair strawberry with easy sprouting stolon, the offspring is selected to have easy sprouting stolon, easy tillering and only flowering, and the strain is transplanted and applied to orchards and woodland, so that the growth of weeds can be inhibited.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The present application relates to cherry molecular marker assisted germplasm identification and breeding technical field, specifically to a kind of InDel marker for identifying Chinese cherry and European sweet cherry germplasm and its application, suitable for identifying cherry resources of Chinese cherry and European sweet cherry two species and interspecific hybrid thereof.The present application designs specific PCR primer using InDel sequence information of cherry genome Cp2_35932912 site, extracts Chinese cherry and European sweet cherry genomic DNA, carries out PCR amplification and polyacrylamide gel electrophoresis, reads electrophoresis band, identifies Chinese cherry, European sweet cherry and interspecific hybrid thereof according to band position and number, develops InDel molecular marker, which can be used for Chinese cherry and European sweet cherry germplasm identification, and also can be used for early identification of interspecific hybridization offspring hybrid authenticity, and the result is accurate and reliable.

This disclosure relates to alloploidy plants and hybrid alloploidy plants having desirable properties such as resistance to abiotic or biological stressors, which can be used as rootstock for cultivated tomato varieties. This disclosure further relates to composite plants that include the alloploidy plants described herein as rootstock. This disclosure further relates to methods for producing alloploidy plants and plant parts, as well as hybrid alloploidy plants and plant parts. In some embodiments, the techniques described herein relate to methods for producing unnatural stress-tolerant composite tomato plants, comprising providing a stress-tolerant allotetraploid tomato plant as rootstock, providing a Solanum lycopersicum variety as scion, grafting the scion onto the rootstock, thereby producing an unnatural stress-tolerant composite tomato plant.

The present disclosure relates to Cucurbitaceae allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite Cucurbitaceae plants comprising the allopoly ploid plants described herein as the rootstock. The disclosure further relates to methods of producing Cucurbitaceae allopolyploid plants and hybrid allopolyploid plants.

The invention discloses a breeding method for creating acetolactate synthase herbicide-resistant brassica napus through sweet and white interspecific hybridization. The breeding method comprises the following steps: performing acetolactate synthase ALS herbicide tolerance screening on the brassica napus; the method comprises the following steps: carrying out BnALS3 gene sequence identification on fresh and tender leaves of a tolerant Brassica campestris L. resource material MYC-1 in a seedling stage; the tolerance characteristic of acetolactate synthase ALSALS herbicides of a brassica napus resource material MYC-1 is introduced into brassica napus, and a brassica napus parent material resistant to ALS herbicides is obtained. By utilizing the method disclosed by the invention, the tolerance of the brassica napus to acetolactate synthase ALS herbicides can be improved.

The application provides a breeding method for interspecific hybridization of new varieties of Iris, comprising the following steps: 1) selecting yellow flag as the female parent and flower flag as the male parent; 2) opening the drooping petals of the yellow flag, removing the anthers under the style, and sealing the whole stigma with a bag before the yellow flag bud opens and the stamens are not yet shedding pollen; 3) picking the stamens and collecting the pollen for standby use when the stamens of the flower flag begin to shed pollen; 4) selecting the pollination time, opening the bag, smearing solution 1 on the stigma of the yellow flag, smearing the pollen of the flower flag on the stigma, spraying solution 2 on the stigma after pollination, and then sealing the pollinated stigma with a bag, wherein the solution 1 is a GA3 aqueous solution, and the solution 2 is a mixed aqueous solution of sucrose and boric acid; 5) removing the bag after the stigma wilts; 6) harvesting the hybrid seeds after the fruits mature; and 7) subjecting the hybrid seeds in the step 6) to the processes of sowing, seedling raising and transplanting, and finally obtaining the hybrid new variety in bloom.

This invention discloses PCR primers, interspecific hybridization methods, and applications for identifying interspecific hybrid offspring of swimming crabs, belonging to the field of aquatic animal genetic engineering technology. The PCR primers of this invention include an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2. When using these PCR primers for PCR amplification, a 786 bp band indicates *Portunus trituberculatus*; a 632 bp band indicates *Portunus cerana*; and a 963 bp band indicates *Portunus rubrum*. The hybrid offspring simultaneously exhibit two specific amplification bands, thus achieving rapid and accurate identification of the hybrid offspring. This invention establishes for the first time a method for identifying hybrid offspring based on PCR amplification fragment length polymorphism, which has advantages such as simple operation, low cost, high accuracy, and suitability for large-scale detection. It can be applied to the fields of swimming crab germplasm innovation, new variety breeding, and genetic improvement.

This invention discloses a method for creating a flavorful sweet and sour melon through interspecific hybridization of thick-skinned melon and cucumber. Using the thick-skinned melon inbred line 'Baipicui' as the female parent and the cucumber inbred line 'Jinyou 406' as the male parent, artificial pollination and hybridization are performed to obtain interspecific hybrid offspring. These offspring are then subjected to several generations of self-pollination and selection to obtain the flavorful sweet and sour melon. This invention is the first to utilize interspecific hybridization between high-quality thick-skinned melon and cucumber to create a new high-quality flavorful sweet and sour melon germplasm. This new germplasm has a moderate sweet and sour taste, a well-proportioned fruit shape, and green flesh, and can be directly used for melon variety improvement.

The application provides a specific molecular marker of Malus sieversii non-stinging No.1 non-stinging strain and application thereof, and belongs to the technical field of crop molecular breeding. The application can simply and quickly identify the non-stinging No.1 non-stinging strain in Malus sieversii materials by simultaneously detecting two marker sites through one double-PCR, and using two InDel markers, and meanwhile, the simultaneous detection of the two marker sites can ensure the accuracy, effectiveness and reliability of the detection results by mutual verification and mutual testing, so that the application has great application value in the aspects of distinguishing Malus sieversii varieties and identifying interspecific hybrid seeds, and can provide technical support for innovative large-fruit non-stinging fresh-eating Malus sieversii varieties. In addition, the application can shorten the breeding cycle of non-stinging Malus sieversii and accelerate the breeding process by extracting leaf genomic DNA of the seedling of the breeding material at the seedling stage for double-PCR amplification, and identifying whether it is the non-stinging No.1 non-stinging strain by observing electrophoresis bands.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.

The invention relates to the technical field of crop breeding, and particularly discloses a water melon and towel gourd crossbreeding method which comprises the following steps: S1, parent selection and cultivation: taking a high-yield tender variety of water melons and a variety of towel gourds with strong disease resistance and obvious furrows as parents, and performing parent cultivation on the parents; s2, parent hybridization: performing hybrid pollination by taking the water melon as a female parent and the towel gourd as a male parent; or performing cross pollination by taking the towel gourd as a female parent and the water melon as a male parent; or carrying out selfing pollination on the watermelons or the towel gourds; s3, seed cultivation: S3-1, management after pollination; (S3-2) reserving hybrid fruits; and S3-3, collecting the hybridized and self-propagated seeds. According to the method, the feasibility of interspecific hybridization of luffa cylindrica (common luffa) and luffa cylindrica (luffa with ridges) is broken through, the limitation of reproductive isolation is broken through, two different hybrid F1-generation seeds are obtained, the excellent hereditary characteristics, such as high yield, disease resistance, stress resistance and high quality, of the luffa cylindrica and the luffa cylindrica are integrated, and a new variety with comprehensive advantages is cultivated.

The invention relates to the field of plant conservation and breeding optimization, in particular to a hybrid embryo rescue method and a method for creating a large number of interspecific hybrid seedlings of Populus nigra by using the hybrid embryo rescue method. The method comprises the following steps: establishing an embryo rescue system, namely observing a separation layer between infructescence and branches after pollination for about 2 months, cutting part of infructescence to develop the embryo rescue system, washing capsules with running water for 120 minutes, lightly wiping peels with 75% alcohol for about 2 minutes, washing the capsules with sterile water for 5-6 times in a super clean bench, lightly absorbing moisture on the surfaces of the capsules with sterile filter paper, and taking out the capsules; the method comprises the following steps of: cutting a capsule by using a sterile scalpel, thoroughly removing floc, carefully clamping seeds by using sterile tweezers, putting the seeds on a 1 / 2MS culture medium containing auxin analogues for embryo rescue, and screening out an optimal culture medium which is 1 / 2MS + 0.06 mg / L naphthylacetic acid (NAA) + 0.08 mg / L indolebutyric acid (IBA) and has the pH value of 5.8-6.0 by adopting a culture dish germination method and taking 1 / 2 MS as the culture medium.

The application discloses a high-pungent pepper variety breeding analysis method, and relates to the technical field of pepper variety breeding, and comprises the following steps: S1: interspecific hybridization is carried out on Chinese pepper and annual pepper to breed new pepper varieties with high pungency; S2: observation results are analyzed, pepper capsaicin is determined, and a pepper strain with optimal disease and pest resistance and capsaicin is screened out; the high-pungent pepper variety breeding analysis method tests the disease and pest resistance of the pepper by releasing diseases and pests in the planting experimental area during the cultivation of the pepper, collects the pepper during the testing of the disease and pest resistance of the pepper, records the observation results of the disease and pest resistance of the pepper, analyzes the observation results, and screens out high-probability high-quality combinations in the pepper matching condition, so that the disease and pest resistance of the pepper matching combinations analyzed by the pepper variety breeding is good.

Provided herein is computational tool design for analyzing allele-specific DNA methylation patterns in in-crosses of inter-strain and inter-specific crosses of organisms. The tool can be incorporated into a system for analyzing allele-specific DNA methylation patterns. allele-specific DNA methylation patterns in in-crosses of inter-strain and inter-specific crosses of organisms is used for analyzing inheritance of epigenetic patterns including for identifying Mendelian and non-Mendelian patterns of epigenetic inheritance. The systems described herein can be used in methods of screening a subject for susceptibility to a disease or condition, and in methods of generating a prognosis of a human subject's susceptibility to a disease or condition.

The invention discloses a specific SNP (Single Nucleotide Polymorphism) locus combination for identifying red and white pitaya and hybrids, which comprises 10 SNP loci, and the 10 SNP loci are obtained by screening through multiple standards such as FST, allele frequency difference and LD pruning on the basis of SLAF-seq data of 45 core germplasms. The specific SNP site combination can be used for accurately distinguishing red-pulp pitaya, white-pulp pitaya and filial generations thereof, the problem of germplasm identification caused by phenotype confusion and interspecific hybridization is solved, and an accurate, efficient and low-cost molecular identification tool is provided for pitaya germplasm resource management, parent selection, molecular breeding and the like. The invention also discloses a primer for amplifying the specific SNP sites and application of the specific SNP site combination.

The application discloses a planting method for interspecies of Tibet white Ganoderma lucidum and Morchella, and comprises the following steps: S1, strain selection and culture: the white Ganoderma lucidum is obtained by a tissue separation method, and is gradually purified and expanded to ensure that mycelium is robust; the Morchella is obtained by a spore separation method or a tissue separation method, is activated by using a PDA culture medium, and is then transferred to a culture medium containing humus and wheat bran with a volume ratio of 2:1 to be expanded; S2, planting preparation: land preparation: a land with flat terrain and facing the sun is selected, weeds and stones are removed, deep ploughing is performed to 20-30 cm, 3 tons of mature organic fertilizer is applied per mu, and after deep ploughing and mixing, the land is leveled.The interspecies method of the white Ganoderma lucidum and the Morchella fully utilizes the difference in the requirement for environmental conditions in the growth process of the two, and realizes complementary utilization of resources; the interspecies method not only improves the land utilization rate per unit area, but also protects the ecological environment by reducing the use of chemical fertilizers and pesticides.

The invention is applicable to the technical field of molecular biology, and provides an InDel marker and a primer group for identifying species of sika deer and red deer and application of the InDel marker and the primer group, the InDel marker is located at a 6116106 site of a fifth chromosome NC057819.1 of the red deer, 120bp of a DNA sequence shown in a sequence table SEQ ID NO: 1 is deleted at the site, and the DNA sequence exists in the sika deer. The invention also provides a primer group for identifying species of sika deer and red deer. The primer group comprises an upstream primer and a downstream primer which are designed aiming at the InDel marker. Based on high-throughput whole genome data of the sika deer and the red deer, the interspecific InDel marker is screened, a corresponding genetic typing technology is established, a technical system which is efficient in detection, low in cost and suitable for large-scale sample screening is constructed, accurate identification of the sika deer, the red deer and interspecific hybrid individuals can be achieved, and better application value is achieved.

The application relates to a method for identifying Brassica rapa interspecific hybrid allopolyploids based on InDel technology and a primer pair. The method comprises the following steps: obtaining a sample to be identified, wherein the sample is obtained by performing interspecific hybridization with Brassica rapa '72049' as a female parent and Eruca vesicaria 'ASTRO' as a male parent, and then performing embryo rescue and chromosome doubling; taking genomic DNA of the material as a template, and performing amplification by using an InDel primer pair; performing gel electrophoresis on the amplified product; and observing an electrophoresis strip to determine the authenticity of the allopolyploid.

The present application firstly obtains the diploid Fujian oyster common, shell gold and shell black strains with fast growth and pure shell color through population breeding. Secondly, the tetraploid Fujian oyster common, shell gold and shell black strains with high and stable tetraploid rate and stable shell color inheritance are obtained through ploidy breeding. Then, the tetraploid populations of long oyster 'Haida 1', 'Haida 2' and 'Haida 3' are obtained by using ploidy breeding technology with the pure shell color, shell type and large size of the individuals as parents. Finally, the fast-growing and high-temperature-resistant hybrid triploid oyster new strains with different shell colors are obtained through interspecific hybridization. The hybrid triploid oyster new strains obtained in the present application maintain the fast growth and strong stress resistance of long oyster, and have the excellent high-temperature-resistant traits of Fujian oyster. In addition, the shell color of the gold and black hybrid triploid oyster new strains is pure and the color is beautiful. The hybrid triploid oyster new strains obtained in the present application expand the germplasm resources of the shell color strains of the cultured oyster, provide important germplasm resources for the high-quality development and transformation and upgrading of the oyster industry to the high-end consumer market, and have a broad industrial application prospect.

The application belongs to the technical field of biological breeding, and specifically discloses a branch fungus transcription factor SycMYB6 and application thereof in regulating drought stress tolerance of plants, and specifically provides application of overexpressed transcription factor SycMYB6 or a protein coded by the transcription factor SycMYB6 in breeding drought-resistant crop germplasm, and a nucleotide sequence of the transcription factor SycMYB6 is shown as SEQ ID NO. 1. The application can improve drought resistance of crops and breed excellent germplasm resources by overexpressing the transcription factor SycMYB6 gene in crops through genetic engineering, and compared with traditional breeding, the technology has short cycle, clear target, is not limited by intergeneric cross, and greatly improves the efficiency of breeding drought-resistant crop germplasm.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

This invention discloses a method for interspecific hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and drying them, placing them in seawater, continuously aerating and artificially circulating the water to induce natural sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a sperm-containing water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a seedling pond; and cultivating the fertilized eggs in seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine pearl oyster germplasm and industrial upgrading.

A molecular marker for identifying Vitis vinifera and its interspecific hybrid grapes and an application thereof are provided. Through whole-genome resequencing data analysis, a significant InDel locus IH1 is identified, which is located at 7,224,357 base pairs (bp) on chromosome 13 of grapes. This locus shows genotypic differences between Vitis vinifera and its interspecific hybrids. Through the differences in the number and distribution of electrophoretic bands, Vitis vinifera and its interspecific hybrids may be distinguished. Based on the characteristics of this molecular marker, it may be used for screening of interspecific hybrid progeny lines in the early stage of breeding and elimination of selfed progeny. At the same time, it may also conduct screening of the genetic background of seedlings.

The invention provides a breeding method of white flower triploid bolting vegetables, which comprises the following steps: taking allotetraploid, white flower and self-incompatible bolting materials' YT25011 'and' YT25018 'as female parents, and taking a selfing line' CCX2500 'obtained by selfing and separating late flowering cabbage offspring of yellow flower and diploid variety' Zhengcheng No.1 'which are planted in a large production area in the unit as male parents; the method comprises the following steps: respectively hybridizing two male parents with a female parent when the female parent and the male parent simultaneously bloom, reserving seeds for the male parent and the female parent, and hybridizing self-incompatible young Chinese cabbage materials' YT25011 'and' YT25018 'with the male parent to obtain the novel triploid sterile vegetable material for the white flower young Chinese cabbage. The method is characterized in that 4mM of disodium ethylene diamine tetraacetate solution is applied to pistil stigmas of the young Chinese cabbage to overcome distant hybridization incompatibility; the obtained vegetables for bolting are triploid sterile offspring obtained by hybridizing diploid and tetraploid, and the obtained triploid vegetables for bolting are high in field performance stress resistance, can be planted all year round, and are excellent in agronomic character and remarkable in interspecific hybridization advantage.

The present application relates to the field of plant biotechnology, in particular to a method for rescuing seedlings from Malus robusta and Malus baccata far-off hybridization zygotes and application, and the method is summarized as follows: a method for rescuing seedlings from Malus robusta and Malus baccata far-off hybridization zygotes is provided.The present application carries out interspecific far-off hybridization by taking Malus robusta as female parent and Malus baccata as male parent.According to the degeneration of far-off hybridization zygotes, the zygotes are rescued.The present application determines the suitable sampling period for rescuing zygotes of hybrid combinations, the concentration and ratio of exogenous hormones required for zygote germination, proliferation and expansion, rooting culture, and the suitable hardening and transplanting method.The zygote rescue system for interspecific hybridization of Malus robusta and Malus baccata established by the present application has a zygote germination rate of 74.3%, a proliferation coefficient of 5.1, a rooting rate of 95.5% in rooting culture, and a hardening and transplanting survival rate of 96.4%.

The invention belongs to the technical field of biology, and particularly relates to a method for positioning sex determining genes of hybrid generations among species. The method comprises the following steps: (1) constructing a genetic positioning group; and (2) carrying out bioinformatics analysis on the genetic positioning population containing the genetic marker. Wherein the separation mode of the genetic marker mined between the two species populations in the F1BC1 generation population is analyzed by using a bioinformatics method, and the chromosome and the locus where the sex determination gene is located are judged according to the interval of the genetic marker on the genome significantly deviating from an expected value. The positioning analysis method disclosed by the invention is strong in practicability and high in accuracy, and can be used for positioning sex determining genes of filial generations among diploid species and comparative biological research.

The application provides a method for identifying F1 hybrids of interspecific cross of Betula nigra and Betula fukienensis by using SSR molecular markers, and relates to the technical field of biology.The application is a method for identifying F1 hybrids of interspecific cross of Betula nigra and Betula fukienensis by using SSR molecular markers, which uses the SSR marker technology to perform PCR amplification on the DNA of F1 hybrids of interspecific cross of Betula, uses capillary electrophoresis to detect the amplification products, and combines the bands and the plant morphology to determine the authenticity of the F1 hybrids.The SSR marker primer provided by the application has good repeatability and strong specificity;the method has the advantages of high efficiency, low cost, short cycle and early screening, can replace the traditional hybrid identification method, realizes accurate identification of F1 hybrids of interspecific cross of Betula nigra and Betula fukienensis, and has important practical application value.