The invention discloses a tissue culture rapid propagation method of an ornamental lily The method comprises the following steps: selecting a scale without injuries at an intermediate layer of the ornamental lily, washing and cleaning the scale with clear water, sequentially processing the cleaned scale with 75% alcohol and 0.1% mercuric chloride, and then washing with sterile water for 5-7 times; cutting the scale which is well sterilized into small pieces with the area of 1-2cm<2>, inoculating the small pieces onto an induction culture medium, performing induction for 23-27 days under induction conditions, and budding and becoming a patch of bulb; cutting 2-3 single buds from the induced patch of bulb, and transferring to a propagation culture medium for performing propagation culture for 25-35 days; selecting the clumpy buds with the blade height of 2-4cm from the propagated patch of bulb, cutting into the single buds and inoculating the single buds onto a rooting culture medium for culturing and rooting for 20-26 days so as to become test-tube plantlets; and performing acclimatization on the test-tube plantlets, then cleaning, and transplanting into a nutrient matrix formed by mixing yellow soil with vermiculite in a volume ratio of 1: 1 for cultivation and management. By adopting the method, the propagation coefficient is increased, the propagation speed is accelerated, the effects of detoxification and rejuvenation are achieved, and the problems of degradation, incompatibility of interspecific hybridization and the like, which are often caused by multi-generation propagation, can be avoided.