42 results about "Interspecific hybridization" patented technology

The invention discloses a molecular marker closely linked with a thin-skinned muskmelon bacterial fruit blotch resistance gene and application thereof, and belongs to the field of muskmelon disease resistance identification. According to the method, a disease-resistant muskmelon strain and a susceptible muskmelon strain are subjected to interspecific hybridization, genetic analysis is carried out on BFB-resistant characters of muskmelons by utilizing an F2 group, a high-resistance gene pool and a high-susceptibility gene pool are constructed on the basis, and resistance candidate genes of the muskmelons are mined by utilizing a BSA-seq technology and a molecular marker technology. Research results can provide a research basis for disease resistance character gene mining and mechanism research, and the method has important scientific significance for screening and application of muskmelon disease-resistant germplasm resources. The molecular marker KASP988272 is developed according to the genotype difference between candidate gene parents, and the genotype identification accuracy of the molecular marker in an F2 group is as high as 80%. The developed molecular marker closely linked with the bacterial fruit blotch resistant gene is used for rapidly and efficiently identifying the bacterial fruit blotch resistant thin-skinned muskmelon variety, and a technical support is provided for selection of the bacterial fruit blotch resistant thin-skinned muskmelon variety.

The present invention discloses a molecular marker for identifying Vitis vinifera and its interspecific hybrid grapes and its application, belonging to the technical field of biological breeding. Through the analysis of whole-genome resequencing data, the present invention identifies a significant InDel locus IH1, which is located at 7,224,357 bp on chromosome 13 of grapes. This locus shows significant genotypic differences between Vitis vinifera grapes and their interspecific hybrids. Through the differences in the number and distribution of electrophoretic bands, Vitis vinifera grapes and their interspecific hybrids can be visually and accurately distinguished. Based on the characteristics of this molecular marker, it can be used for rapid screening of interspecific hybrid offspring lines in the early stage of breeding to eliminate self-crossed offspring. At the same time, it can also rapidly screen the genetic background of seedlings to ensure the accuracy of seedlings, which plays an important role in the early screening of breeding offspring and the screening of the genetic background of seedlings.

The present invention discloses a set of SSR molecular markers for identifying wild chrysanthemum and Shennong fragrant chrysanthemum germplasm resources, consisting of 21 pairs of SSRs numbered SSR01-SSR21. The primer sequences for amplifying these 21 pairs of molecular markers are shown in SEQ ID NO: 1-42, and the present invention belongs to the field of molecular biology technology. Using these 21 pairs of SSR molecular markers, the collected wild chrysanthemum and Shennong fragrant chrysanthemum can be identified and the germplasm genetic diversity analyzed. The present invention uses SSR markers for the first time to jointly detect the genetic diversity of wild chrysanthemum and Shennong fragrant chrysanthemum germplasm. This set of SSR molecular markers has good polymorphism and a high level of genetic diversity. It can be used in the fields of kinship analysis, resource genetic evaluation, identification of interspecific hybrid offspring, positioning of trait genes, and molecular marker-assisted breeding of wild chrysanthemum and Shennong fragrant chrysanthemum.

The application provides a method for creating a strawberry germplasm for controlling weeds and application thereof, and belongs to the technical field of biological breeding. Forest strawberry 'Ruegen' is used as a female parent, and yellow hair strawberry is used as a male parent, F1 generation is obtained by hybridization, F2 generation is obtained by screening selfing of the F1 generation, and the F2 generation is screened to obtain a strain with less flowering, no fruiting, easy to sprout stolon and easy to tiller, which is the strawberry for controlling weeds. The forest strawberry 'Ruegen' with strong resistance and not sprouting stolon is crossbred with the yellow hair strawberry with easy sprouting stolon, the offspring is selected to have easy sprouting stolon, easy tillering and only flowering, and the strain is transplanted and applied to orchards and woodland, so that the growth of weeds can be inhibited.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The present invention discloses a method for identifying interspecific hybrid germplasm of Rubus rubus, comprising the following steps: using hexaploid cultivated blackberry as the female parent and diploid wild Rubus rubus as the male parent, obtaining hybrid seeds, and cultivating interspecific hybrid seedlings; designing male parent SSR primers, amplifying the DNA of the interspecific hybrid seedlings, and screening primer combinations to identify true hybrids; verifying the amplified bands of the screened SSR primer pairs in wild Rubus rubus, raspberry, and blackberry varieties, and optimizing specific primers for identifying hybrid offspring of different wild Rubus male parents. The screened SSR primers are used to identify blackberry cultivars and interspecific hybrid seedlings of Rubus rubus, and the true hybrids are identified and the parental germplasm sources are confirmed based on the amplified bands. The present invention successfully hybridizes diploid wild Rubus rubus with hexaploid cultivated blackberry varieties for the first time, uses the SSR molecular markers obtained through screening to identify hybrids, and verifies that the wild germplasm has entered the cultivated varieties through hybridization.

The present application relates to cherry molecular marker assisted germplasm identification and breeding technical field, specifically to a kind of InDel marker for identifying Chinese cherry and European sweet cherry germplasm and its application, suitable for identifying cherry resources of Chinese cherry and European sweet cherry two species and interspecific hybrid thereof.The present application designs specific PCR primer using InDel sequence information of cherry genome Cp2_35932912 site, extracts Chinese cherry and European sweet cherry genomic DNA, carries out PCR amplification and polyacrylamide gel electrophoresis, reads electrophoresis band, identifies Chinese cherry, European sweet cherry and interspecific hybrid thereof according to band position and number, develops InDel molecular marker, which can be used for Chinese cherry and European sweet cherry germplasm identification, and also can be used for early identification of interspecific hybridization offspring hybrid authenticity, and the result is accurate and reliable.

This disclosure relates to alloploidy plants and hybrid alloploidy plants having desirable properties such as resistance to abiotic or biological stressors, which can be used as rootstock for cultivated tomato varieties. This disclosure further relates to composite plants that include the alloploidy plants described herein as rootstock. This disclosure further relates to methods for producing alloploidy plants and plant parts, as well as hybrid alloploidy plants and plant parts. In some embodiments, the techniques described herein relate to methods for producing unnatural stress-tolerant composite tomato plants, comprising providing a stress-tolerant allotetraploid tomato plant as rootstock, providing a Solanum lycopersicum variety as scion, grafting the scion onto the rootstock, thereby producing an unnatural stress-tolerant composite tomato plant.

The present disclosure relates to Cucurbitaceae allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite Cucurbitaceae plants comprising the allopoly ploid plants described herein as the rootstock. The disclosure further relates to methods of producing Cucurbitaceae allopolyploid plants and hybrid allopolyploid plants.

The invention discloses a breeding method for creating acetolactate synthase herbicide-resistant brassica napus through sweet and white interspecific hybridization. The breeding method comprises the following steps: performing acetolactate synthase ALS herbicide tolerance screening on the brassica napus; the method comprises the following steps: carrying out BnALS3 gene sequence identification on fresh and tender leaves of a tolerant Brassica campestris L. resource material MYC-1 in a seedling stage; the tolerance characteristic of acetolactate synthase ALSALS herbicides of a brassica napus resource material MYC-1 is introduced into brassica napus, and a brassica napus parent material resistant to ALS herbicides is obtained. By utilizing the method disclosed by the invention, the tolerance of the brassica napus to acetolactate synthase ALS herbicides can be improved.

The application provides a breeding method for interspecific hybridization of new varieties of Iris, comprising the following steps: 1) selecting yellow flag as the female parent and flower flag as the male parent; 2) opening the drooping petals of the yellow flag, removing the anthers under the style, and sealing the whole stigma with a bag before the yellow flag bud opens and the stamens are not yet shedding pollen; 3) picking the stamens and collecting the pollen for standby use when the stamens of the flower flag begin to shed pollen; 4) selecting the pollination time, opening the bag, smearing solution 1 on the stigma of the yellow flag, smearing the pollen of the flower flag on the stigma, spraying solution 2 on the stigma after pollination, and then sealing the pollinated stigma with a bag, wherein the solution 1 is a GA3 aqueous solution, and the solution 2 is a mixed aqueous solution of sucrose and boric acid; 5) removing the bag after the stigma wilts; 6) harvesting the hybrid seeds after the fruits mature; and 7) subjecting the hybrid seeds in the step 6) to the processes of sowing, seedling raising and transplanting, and finally obtaining the hybrid new variety in bloom.

This invention discloses PCR primers, interspecific hybridization methods, and applications for identifying interspecific hybrid offspring of swimming crabs, belonging to the field of aquatic animal genetic engineering technology. The PCR primers of this invention include an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2. When using these PCR primers for PCR amplification, a 786 bp band indicates *Portunus trituberculatus*; a 632 bp band indicates *Portunus cerana*; and a 963 bp band indicates *Portunus rubrum*. The hybrid offspring simultaneously exhibit two specific amplification bands, thus achieving rapid and accurate identification of the hybrid offspring. This invention establishes for the first time a method for identifying hybrid offspring based on PCR amplification fragment length polymorphism, which has advantages such as simple operation, low cost, high accuracy, and suitability for large-scale detection. It can be applied to the fields of swimming crab germplasm innovation, new variety breeding, and genetic improvement.

The present invention provides a method for identifying progeny of interspecific hybrids between walnut and Hebei walnut, relating to the technical field of plant genetic breeding. The present invention provides the use of specific primers in identifying progeny of interspecific hybrids between walnut and Hebei walnut, as well as a method for identifying progeny of interspecific hybrids between walnut and Hebei walnut. The method is simple to operate, has good repeatability, high stability, and is unaffected by the external environment. The method effectively improves the authenticity of hybrid offspring between walnut varieties, increases the efficiency of early selection of hybrid offspring, shortens the breeding cycle, and improves breeding efficiency.

This invention discloses a method for creating a flavorful sweet and sour melon through interspecific hybridization of thick-skinned melon and cucumber. Using the thick-skinned melon inbred line 'Baipicui' as the female parent and the cucumber inbred line 'Jinyou 406' as the male parent, artificial pollination and hybridization are performed to obtain interspecific hybrid offspring. These offspring are then subjected to several generations of self-pollination and selection to obtain the flavorful sweet and sour melon. This invention is the first to utilize interspecific hybridization between high-quality thick-skinned melon and cucumber to create a new high-quality flavorful sweet and sour melon germplasm. This new germplasm has a moderate sweet and sour taste, a well-proportioned fruit shape, and green flesh, and can be directly used for melon variety improvement.

The application provides a specific molecular marker of Malus sieversii non-stinging No.1 non-stinging strain and application thereof, and belongs to the technical field of crop molecular breeding. The application can simply and quickly identify the non-stinging No.1 non-stinging strain in Malus sieversii materials by simultaneously detecting two marker sites through one double-PCR, and using two InDel markers, and meanwhile, the simultaneous detection of the two marker sites can ensure the accuracy, effectiveness and reliability of the detection results by mutual verification and mutual testing, so that the application has great application value in the aspects of distinguishing Malus sieversii varieties and identifying interspecific hybrid seeds, and can provide technical support for innovative large-fruit non-stinging fresh-eating Malus sieversii varieties. In addition, the application can shorten the breeding cycle of non-stinging Malus sieversii and accelerate the breeding process by extracting leaf genomic DNA of the seedling of the breeding material at the seedling stage for double-PCR amplification, and identifying whether it is the non-stinging No.1 non-stinging strain by observing electrophoresis bands.

The invention provides a method for producing tetraploid oysters with different shell colors through interspecific hybridization. The method comprises the following steps: firstly, selecting Fujian oysters and crassostrea gigas with common shell color, golden shell color and black shell color and excellent growth traits as parents, and respectively obtaining triploid and tetraploid individuals through a chemical induction technology; and then carrying out continuous 2-4 generations of population breeding to obtain Fujian oyster and crassostrea gigas tetraploid populations with stable ploidy and different shell color characteristics. Then, the tetraploid populations are hybridized to obtain hybrid tetraploid oysters with different shell colors, and the hybrid tetraploid oysters have three shell colors including a common shell color, a gold shell color and a black shell color. The produced tetraploid oysters have high growth speed, high ploidy stability and high survival rate, and excellent germplasm resources can be provided for the triploid oyster industry. The method not only improves the cultivation benefits of the oysters, but also has wide industrial application prospects. The method combines the technologies of selective breeding, polyploid breeding, cross breeding and the like, breaks through the limitation of a traditional breeding method, has higher breeding efficiency and application potential, is particularly suitable for oyster breeding in coastal areas of China, and promotes the oyster industry to be transformed and upgraded to a high-end consumer market.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.

The invention relates to the technical field of crop breeding, and particularly discloses a water melon and towel gourd crossbreeding method which comprises the following steps: S1, parent selection and cultivation: taking a high-yield tender variety of water melons and a variety of towel gourds with strong disease resistance and obvious furrows as parents, and performing parent cultivation on the parents; s2, parent hybridization: performing hybrid pollination by taking the water melon as a female parent and the towel gourd as a male parent; or performing cross pollination by taking the towel gourd as a female parent and the water melon as a male parent; or carrying out selfing pollination on the watermelons or the towel gourds; s3, seed cultivation: S3-1, management after pollination; (S3-2) reserving hybrid fruits; and S3-3, collecting the hybridized and self-propagated seeds. According to the method, the feasibility of interspecific hybridization of luffa cylindrica (common luffa) and luffa cylindrica (luffa with ridges) is broken through, the limitation of reproductive isolation is broken through, two different hybrid F1-generation seeds are obtained, the excellent hereditary characteristics, such as high yield, disease resistance, stress resistance and high quality, of the luffa cylindrica and the luffa cylindrica are integrated, and a new variety with comprehensive advantages is cultivated.

The invention relates to the field of plant conservation and breeding optimization, in particular to a hybrid embryo rescue method and a method for creating a large number of interspecific hybrid seedlings of Populus nigra by using the hybrid embryo rescue method. The method comprises the following steps: establishing an embryo rescue system, namely observing a separation layer between infructescence and branches after pollination for about 2 months, cutting part of infructescence to develop the embryo rescue system, washing capsules with running water for 120 minutes, lightly wiping peels with 75% alcohol for about 2 minutes, washing the capsules with sterile water for 5-6 times in a super clean bench, lightly absorbing moisture on the surfaces of the capsules with sterile filter paper, and taking out the capsules; the method comprises the following steps of: cutting a capsule by using a sterile scalpel, thoroughly removing floc, carefully clamping seeds by using sterile tweezers, putting the seeds on a 1 / 2MS culture medium containing auxin analogues for embryo rescue, and screening out an optimal culture medium which is 1 / 2MS + 0.06 mg / L naphthylacetic acid (NAA) + 0.08 mg / L indolebutyric acid (IBA) and has the pH value of 5.8-6.0 by adopting a culture dish germination method and taking 1 / 2 MS as the culture medium.

The application discloses a high-pungent pepper variety breeding analysis method, and relates to the technical field of pepper variety breeding, and comprises the following steps: S1: interspecific hybridization is carried out on Chinese pepper and annual pepper to breed new pepper varieties with high pungency; S2: observation results are analyzed, pepper capsaicin is determined, and a pepper strain with optimal disease and pest resistance and capsaicin is screened out; the high-pungent pepper variety breeding analysis method tests the disease and pest resistance of the pepper by releasing diseases and pests in the planting experimental area during the cultivation of the pepper, collects the pepper during the testing of the disease and pest resistance of the pepper, records the observation results of the disease and pest resistance of the pepper, analyzes the observation results, and screens out high-probability high-quality combinations in the pepper matching condition, so that the disease and pest resistance of the pepper matching combinations analyzed by the pepper variety breeding is good.

Provided herein is computational tool design for analyzing allele-specific DNA methylation patterns in in-crosses of inter-strain and inter-specific crosses of organisms. The tool can be incorporated into a system for analyzing allele-specific DNA methylation patterns. allele-specific DNA methylation patterns in in-crosses of inter-strain and inter-specific crosses of organisms is used for analyzing inheritance of epigenetic patterns including for identifying Mendelian and non-Mendelian patterns of epigenetic inheritance. The systems described herein can be used in methods of screening a subject for susceptibility to a disease or condition, and in methods of generating a prognosis of a human subject's susceptibility to a disease or condition.

The invention discloses a specific SNP (Single Nucleotide Polymorphism) locus combination for identifying red and white pitaya and hybrids, which comprises 10 SNP loci, and the 10 SNP loci are obtained by screening through multiple standards such as FST, allele frequency difference and LD pruning on the basis of SLAF-seq data of 45 core germplasms. The specific SNP site combination can be used for accurately distinguishing red-pulp pitaya, white-pulp pitaya and filial generations thereof, the problem of germplasm identification caused by phenotype confusion and interspecific hybridization is solved, and an accurate, efficient and low-cost molecular identification tool is provided for pitaya germplasm resource management, parent selection, molecular breeding and the like. The invention also discloses a primer for amplifying the specific SNP sites and application of the specific SNP site combination.

The application discloses a planting method for interspecies of Tibet white Ganoderma lucidum and Morchella, and comprises the following steps: S1, strain selection and culture: the white Ganoderma lucidum is obtained by a tissue separation method, and is gradually purified and expanded to ensure that mycelium is robust; the Morchella is obtained by a spore separation method or a tissue separation method, is activated by using a PDA culture medium, and is then transferred to a culture medium containing humus and wheat bran with a volume ratio of 2:1 to be expanded; S2, planting preparation: land preparation: a land with flat terrain and facing the sun is selected, weeds and stones are removed, deep ploughing is performed to 20-30 cm, 3 tons of mature organic fertilizer is applied per mu, and after deep ploughing and mixing, the land is leveled.The interspecies method of the white Ganoderma lucidum and the Morchella fully utilizes the difference in the requirement for environmental conditions in the growth process of the two, and realizes complementary utilization of resources; the interspecies method not only improves the land utilization rate per unit area, but also protects the ecological environment by reducing the use of chemical fertilizers and pesticides.

The invention is applicable to the technical field of molecular biology, and provides an InDel marker and a primer group for identifying species of sika deer and red deer and application of the InDel marker and the primer group, the InDel marker is located at a 6116106 site of a fifth chromosome NC057819.1 of the red deer, 120bp of a DNA sequence shown in a sequence table SEQ ID NO: 1 is deleted at the site, and the DNA sequence exists in the sika deer. The invention also provides a primer group for identifying species of sika deer and red deer. The primer group comprises an upstream primer and a downstream primer which are designed aiming at the InDel marker. Based on high-throughput whole genome data of the sika deer and the red deer, the interspecific InDel marker is screened, a corresponding genetic typing technology is established, a technical system which is efficient in detection, low in cost and suitable for large-scale sample screening is constructed, accurate identification of the sika deer, the red deer and interspecific hybrid individuals can be achieved, and better application value is achieved.

A new and distinct hybrid variety of blueberry (Vaccinium corymbosum x Vaccinium stamineum hybrid), named ‘FL16-754’, which resulted from an interspecific cross between a highbush blueberry cultivar and a tetraploid plant of Vaccinium stamineum. The new blueberry, named ‘FL16-754’, can be distinguished at least by its a very low chilling requirement for both flower buds and vegetative buds, as well as a vigorous, bushy growth habit. ‘FL16-754’ berries are borne in loose clusters, with long pedicels and peduncles, with very firm to crisp, medium to large size, with excellent scar. ‘FL16-754’ is also highly fertile, both as a pollen parent and as a seed parent.

The application relates to a method for identifying Brassica rapa interspecific hybrid allopolyploids based on InDel technology and a primer pair. The method comprises the following steps: obtaining a sample to be identified, wherein the sample is obtained by performing interspecific hybridization with Brassica rapa '72049' as a female parent and Eruca vesicaria 'ASTRO' as a male parent, and then performing embryo rescue and chromosome doubling; taking genomic DNA of the material as a template, and performing amplification by using an InDel primer pair; performing gel electrophoresis on the amplified product; and observing an electrophoresis strip to determine the authenticity of the allopolyploid.

The present invention relates to the field of information technology, and provides a method for uniformly constructing a global weed gene database across platforms, including: screening out weed populations with gene exchange characteristics with target crops, using gene alignment methods to identify gene fragments of gene exchange between crops and weeds, and determining the transmission path of resistance genes in interspecific hybridization; according to the gene alignment results, extracting the transmission path data of resistance genes, and combining with the genetic structure characteristics of the weed population, analyzing the impact of the transmission of resistance genes on the genetic structure of the weed population; calculating the frequency distribution of resistance genes in different weed populations, judging the diffusion trend of resistance genes in the weed population according to the frequency distribution, generating a resistance gene diffusion map, and based on the weed population genetic structure change trend map and the resistance gene diffusion map, predicting the future change trend of the weed population genetic structure, uniformly constructing a global weed gene database, which helps to formulate effective weed management strategies.

The present application firstly obtains the diploid Fujian oyster common, shell gold and shell black strains with fast growth and pure shell color through population breeding. Secondly, the tetraploid Fujian oyster common, shell gold and shell black strains with high and stable tetraploid rate and stable shell color inheritance are obtained through ploidy breeding. Then, the tetraploid populations of long oyster 'Haida 1', 'Haida 2' and 'Haida 3' are obtained by using ploidy breeding technology with the pure shell color, shell type and large size of the individuals as parents. Finally, the fast-growing and high-temperature-resistant hybrid triploid oyster new strains with different shell colors are obtained through interspecific hybridization. The hybrid triploid oyster new strains obtained in the present application maintain the fast growth and strong stress resistance of long oyster, and have the excellent high-temperature-resistant traits of Fujian oyster. In addition, the shell color of the gold and black hybrid triploid oyster new strains is pure and the color is beautiful. The hybrid triploid oyster new strains obtained in the present application expand the germplasm resources of the shell color strains of the cultured oyster, provide important germplasm resources for the high-quality development and transformation and upgrading of the oyster industry to the high-end consumer market, and have a broad industrial application prospect.

The application belongs to the technical field of biological breeding, and specifically discloses a branch fungus transcription factor SycMYB6 and application thereof in regulating drought stress tolerance of plants, and specifically provides application of overexpressed transcription factor SycMYB6 or a protein coded by the transcription factor SycMYB6 in breeding drought-resistant crop germplasm, and a nucleotide sequence of the transcription factor SycMYB6 is shown as SEQ ID NO. 1. The application can improve drought resistance of crops and breed excellent germplasm resources by overexpressing the transcription factor SycMYB6 gene in crops through genetic engineering, and compared with traditional breeding, the technology has short cycle, clear target, is not limited by intergeneric cross, and greatly improves the efficiency of breeding drought-resistant crop germplasm.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.