55 results about "Interspecific hybridization" patented technology

The invention relates to the technical field of information, and provides a cross-platform global weed gene database unified building method which comprises the following steps: screening out a weed population having gene exchange characteristics with a target crop, identifying gene segments of gene exchange between the crop and weeds by adopting a gene comparison method, and determining a transmission path of resistance genes in interspecific hybridization; according to a gene comparison result, extracting transmission path data of the resistance gene, and analyzing the influence of resistance gene transmission on the genetic structure of the weed population in combination with the genetic structure characteristics of the weed population; calculating the frequency distribution of the resistance genes in different weed populations, judging the diffusion trend of the resistance genes in the weed populations according to the frequency distribution, generating a resistance gene diffusion map, and predicting the variation trend of the future weed population genetic structure based on a weed population genetic structure variation trend chart and the resistance gene diffusion map. And a global weed gene database is established, so that an effective weed management strategy can be formulated.

The invention discloses a method for creating a high-frequency introgression Chinese cabbage-cabbage introgression line, and relates to the technical field of introgression breeding. According to the method, tetraploid Chinese cabbage and diploid cabbage are subjected to interspecific hybridization to obtain an allotriploid hybrid (AAC), then the AAC is subjected to hybridization and selfing with diploid Chinese cabbage, and a Chinese cabbage-cabbage introgression line with a high-frequency introgression exogenous species genome sequence is created in combination with free microspore culture. According to the invention, the heterotriploid hybrid mediated genetic system is utilized to realize interspecific high introgression, and a new method is provided for breaking the bottleneck of low exogenous species introgression frequency.

The invention discloses a molecular marker closely linked with a thin-skinned muskmelon bacterial fruit blotch resistance gene and application thereof, and belongs to the field of muskmelon disease resistance identification. According to the method, a disease-resistant muskmelon strain and a susceptible muskmelon strain are subjected to interspecific hybridization, genetic analysis is carried out on BFB-resistant characters of muskmelons by utilizing an F2 group, a high-resistance gene pool and a high-susceptibility gene pool are constructed on the basis, and resistance candidate genes of the muskmelons are mined by utilizing a BSA-seq technology and a molecular marker technology. Research results can provide a research basis for disease resistance character gene mining and mechanism research, and the method has important scientific significance for screening and application of muskmelon disease-resistant germplasm resources. The molecular marker KASP988272 is developed according to the genotype difference between candidate gene parents, and the genotype identification accuracy of the molecular marker in an F2 group is as high as 80%. The developed molecular marker closely linked with the bacterial fruit blotch resistant gene is used for rapidly and efficiently identifying the bacterial fruit blotch resistant thin-skinned muskmelon variety, and a technical support is provided for selection of the bacterial fruit blotch resistant thin-skinned muskmelon variety.

The present invention discloses a molecular marker for identifying Vitis vinifera and its interspecific hybrid grapes and its application, belonging to the technical field of biological breeding. Through the analysis of whole-genome resequencing data, the present invention identifies a significant InDel locus IH1, which is located at 7,224,357 bp on chromosome 13 of grapes. This locus shows significant genotypic differences between Vitis vinifera grapes and their interspecific hybrids. Through the differences in the number and distribution of electrophoretic bands, Vitis vinifera grapes and their interspecific hybrids can be visually and accurately distinguished. Based on the characteristics of this molecular marker, it can be used for rapid screening of interspecific hybrid offspring lines in the early stage of breeding to eliminate self-crossed offspring. At the same time, it can also rapidly screen the genetic background of seedlings to ensure the accuracy of seedlings, which plays an important role in the early screening of breeding offspring and the screening of the genetic background of seedlings.

The present invention discloses a set of SSR molecular markers for identifying wild chrysanthemum and Shennong fragrant chrysanthemum germplasm resources, consisting of 21 pairs of SSRs numbered SSR01-SSR21. The primer sequences for amplifying these 21 pairs of molecular markers are shown in SEQ ID NO: 1-42, and the present invention belongs to the field of molecular biology technology. Using these 21 pairs of SSR molecular markers, the collected wild chrysanthemum and Shennong fragrant chrysanthemum can be identified and the germplasm genetic diversity analyzed. The present invention uses SSR markers for the first time to jointly detect the genetic diversity of wild chrysanthemum and Shennong fragrant chrysanthemum germplasm. This set of SSR molecular markers has good polymorphism and a high level of genetic diversity. It can be used in the fields of kinship analysis, resource genetic evaluation, identification of interspecific hybrid offspring, positioning of trait genes, and molecular marker-assisted breeding of wild chrysanthemum and Shennong fragrant chrysanthemum.

The present invention relates to the technical field of pine hybridization. The present invention provides a method for distant hybridization of the genus Pinus based on stock-scion interaction, comprising the following steps: (1) selecting stock and scion of different tree species for grafting to obtain a grafted plant; (2) cultivating the grafted plant, removing the stamens and bagging before the female flowers of the grafted plant turn white; (3) after the female flowers of the grafted plant turn white, artificial pollination is performed using pollen of a pine tree of the same variety as the stock. The present invention uses pine trees of different factions for grafting and hybridization, and utilizes the horizontal transfer of small molecules between the stock and scion to achieve the possibility of interspecific hybridization, thereby overcoming the problem of highly incompatible hybridization between different subgenera of pine trees.

The application provides a method for creating a strawberry germplasm for controlling weeds and application thereof, and belongs to the technical field of biological breeding. Forest strawberry 'Ruegen' is used as a female parent, and yellow hair strawberry is used as a male parent, F1 generation is obtained by hybridization, F2 generation is obtained by screening selfing of the F1 generation, and the F2 generation is screened to obtain a strain with less flowering, no fruiting, easy to sprout stolon and easy to tiller, which is the strawberry for controlling weeds. The forest strawberry 'Ruegen' with strong resistance and not sprouting stolon is crossbred with the yellow hair strawberry with easy sprouting stolon, the offspring is selected to have easy sprouting stolon, easy tillering and only flowering, and the strain is transplanted and applied to orchards and woodland, so that the growth of weeds can be inhibited.

The present invention discloses a method for developing and applying specific single-locus IP markers in the radish genome. This method utilizes bioinformatics techniques to develop specific single-locus IP markers in radish based on the genomic information of radish and Brassica crops; and these markers are actually applied to the identification of exogenous introgressed fragments in the progeny of radish-Brassica interspecific hybridization. The present invention realizes the rapid identification of excellent gene fragments in the progeny of distant hybridization between radish as the donor and Brassica crops, effectively assists the precise introgression of excellent gene fragments, accurately tracks the size of the gene fragments involved at the same time, breaks the linkage drag of bad traits, and realizes the efficient creation and utilization of germplasm resources. At the same time, it provides important data support for the identification of chromosomal fragments of genes related to improved traits in hybrid progeny.

The invention discloses a method for creating offspring of buckwheat interspecific hybridization. According to the invention, the immature embryo can be successfully obtained through artificial hybridization by taking wild buckwheat as a female parent and tartary buckwheat as a male parent; in order to solve the problems that the induction rate of an immature embryo on a callus induction culture medium is low and the differentiation rate is low when the callus is subjected to differentiation culture in a differentiation culture medium, the callus induction culture medium and the differentiation culture medium are optimized and screened; the obtained callus induction culture medium and the obtained differentiation culture medium remarkably improve the callus induction rate and the differentiation rate during differentiation culture, regenerated plants are obtained at high frequency, colchicine doubling is performed on the regenerated plants on the basis, and a doubled interspecific hybridization new germplasm is obtained by combining morphological and cytological identification. The method is applied to breeding of high-yield stress-resistant, high-flavone, perennial and other breakthrough new varieties or serves as a hybrid bridge parent, and the excellent characters of stress resistance, high flavone, perennial and the like of wild buckwheat are introduced into buckwheat cultivation.

The present invention discloses a method for identifying interspecific hybrid germplasm of Rubus rubus, comprising the following steps: using hexaploid cultivated blackberry as the female parent and diploid wild Rubus rubus as the male parent, obtaining hybrid seeds, and cultivating interspecific hybrid seedlings; designing male parent SSR primers, amplifying the DNA of the interspecific hybrid seedlings, and screening primer combinations to identify true hybrids; verifying the amplified bands of the screened SSR primer pairs in wild Rubus rubus, raspberry, and blackberry varieties, and optimizing specific primers for identifying hybrid offspring of different wild Rubus male parents. The screened SSR primers are used to identify blackberry cultivars and interspecific hybrid seedlings of Rubus rubus, and the true hybrids are identified and the parental germplasm sources are confirmed based on the amplified bands. The present invention successfully hybridizes diploid wild Rubus rubus with hexaploid cultivated blackberry varieties for the first time, uses the SSR molecular markers obtained through screening to identify hybrids, and verifies that the wild germplasm has entered the cultivated varieties through hybridization.

The present application relates to cherry molecular marker assisted germplasm identification and breeding technical field, specifically to a kind of InDel marker for identifying Chinese cherry and European sweet cherry germplasm and its application, suitable for identifying cherry resources of Chinese cherry and European sweet cherry two species and interspecific hybrid thereof.The present application designs specific PCR primer using InDel sequence information of cherry genome Cp2_35932912 site, extracts Chinese cherry and European sweet cherry genomic DNA, carries out PCR amplification and polyacrylamide gel electrophoresis, reads electrophoresis band, identifies Chinese cherry, European sweet cherry and interspecific hybrid thereof according to band position and number, develops InDel molecular marker, which can be used for Chinese cherry and European sweet cherry germplasm identification, and also can be used for early identification of interspecific hybridization offspring hybrid authenticity, and the result is accurate and reliable.

This disclosure relates to alloploidy plants and hybrid alloploidy plants having desirable properties such as resistance to abiotic or biological stressors, which can be used as rootstock for cultivated tomato varieties. This disclosure further relates to composite plants that include the alloploidy plants described herein as rootstock. This disclosure further relates to methods for producing alloploidy plants and plant parts, as well as hybrid alloploidy plants and plant parts. In some embodiments, the techniques described herein relate to methods for producing unnatural stress-tolerant composite tomato plants, comprising providing a stress-tolerant allotetraploid tomato plant as rootstock, providing a Solanum lycopersicum variety as scion, grafting the scion onto the rootstock, thereby producing an unnatural stress-tolerant composite tomato plant.

The invention discloses a breeding method of high-quality pork pigs, and belongs to the technical field of livestock breeding. The breeding method comprises the following steps: by taking Nanjiang pigs and Candian large white pigs as materials, performing selection and retention, cross production and multi-stage selection and retention by establishing selection index rating, and performing cross fixation and generation breeding by adopting a group subculture breeding method, thereby obtaining the Nanjiang pigs and the Candian large white pigs. According to the breeding method disclosed by the invention, interspecific hybridization advantages are fully utilized, the bred high-quality pork pigs not only keep excellent meat quality characteristics of the injiang pigs to the greatest extent, but also greatly improve production performance indexes such as growth speed, feed conversion efficiency and lean meat percentage; high-quality commercial pork pigs with white hair characteristics, high growth speed, high breeding efficiency, excellent meat quality and high meat yield are obtained.

The present disclosure relates to Cucurbitaceae allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite Cucurbitaceae plants comprising the allopoly ploid plants described herein as the rootstock. The disclosure further relates to methods of producing Cucurbitaceae allopolyploid plants and hybrid allopolyploid plants.

The invention discloses a breeding method for creating acetolactate synthase herbicide-resistant brassica napus through sweet and white interspecific hybridization. The breeding method comprises the following steps: performing acetolactate synthase ALS herbicide tolerance screening on the brassica napus; the method comprises the following steps: carrying out BnALS3 gene sequence identification on fresh and tender leaves of a tolerant Brassica campestris L. resource material MYC-1 in a seedling stage; the tolerance characteristic of acetolactate synthase ALSALS herbicides of a brassica napus resource material MYC-1 is introduced into brassica napus, and a brassica napus parent material resistant to ALS herbicides is obtained. By utilizing the method disclosed by the invention, the tolerance of the brassica napus to acetolactate synthase ALS herbicides can be improved.

The application provides a breeding method for interspecific hybridization of new varieties of Iris, comprising the following steps: 1) selecting yellow flag as the female parent and flower flag as the male parent; 2) opening the drooping petals of the yellow flag, removing the anthers under the style, and sealing the whole stigma with a bag before the yellow flag bud opens and the stamens are not yet shedding pollen; 3) picking the stamens and collecting the pollen for standby use when the stamens of the flower flag begin to shed pollen; 4) selecting the pollination time, opening the bag, smearing solution 1 on the stigma of the yellow flag, smearing the pollen of the flower flag on the stigma, spraying solution 2 on the stigma after pollination, and then sealing the pollinated stigma with a bag, wherein the solution 1 is a GA3 aqueous solution, and the solution 2 is a mixed aqueous solution of sucrose and boric acid; 5) removing the bag after the stigma wilts; 6) harvesting the hybrid seeds after the fruits mature; and 7) subjecting the hybrid seeds in the step 6) to the processes of sowing, seedling raising and transplanting, and finally obtaining the hybrid new variety in bloom.

This invention discloses PCR primers, interspecific hybridization methods, and applications for identifying interspecific hybrid offspring of swimming crabs, belonging to the field of aquatic animal genetic engineering technology. The PCR primers of this invention include an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2. When using these PCR primers for PCR amplification, a 786 bp band indicates *Portunus trituberculatus*; a 632 bp band indicates *Portunus cerana*; and a 963 bp band indicates *Portunus rubrum*. The hybrid offspring simultaneously exhibit two specific amplification bands, thus achieving rapid and accurate identification of the hybrid offspring. This invention establishes for the first time a method for identifying hybrid offspring based on PCR amplification fragment length polymorphism, which has advantages such as simple operation, low cost, high accuracy, and suitability for large-scale detection. It can be applied to the fields of swimming crab germplasm innovation, new variety breeding, and genetic improvement.

The invention relates to the technical field of molecular marker-assisted germplasm resource identification and breeding of cherries, in particular to an InDel marker for identifying germplasm of Chinese cherries and European sweet cherries and application of the InDel marker, which are suitable for identifying two species of the Chinese cherries and the European sweet cherries and interspecific hybrid cherry resources of the two species of the Chinese cherries and the European sweet cherries. The InDel molecular marker is developed by designing a specific PCR (Polymerase Chain Reaction) primer by utilizing InDel sequence information of a cherry genome Cp235932912 site, extracting genomic DNA (Deoxyribose Nucleic Acid) of Chinese cherries and European sweet cherries, performing PCR amplification and polyacrylamide gel electrophoresis, reading electrophoretic bands, identifying the Chinese cherries, the European sweet cherries and interspecific hybrids thereof according to the positions and the number of the bands, and identifying the Chinese cherries, the European sweet cherries and the interspecific hybrids of the Chinese cherries, the European sweet cherries and the European sweet cherries according to the InDel molecular marker. The molecular marker can be used for germplasm identification of Chinese cherries and European sweet cherries, and can also be used for early identification of hybrid authenticity of interspecific filial generations of the Chinese cherries and the European sweet cherries, and results are accurate and reliable.

The present invention provides a method for identifying progeny of interspecific hybrids between walnut and Hebei walnut, relating to the technical field of plant genetic breeding. The present invention provides the use of specific primers in identifying progeny of interspecific hybrids between walnut and Hebei walnut, as well as a method for identifying progeny of interspecific hybrids between walnut and Hebei walnut. The method is simple to operate, has good repeatability, high stability, and is unaffected by the external environment. The method effectively improves the authenticity of hybrid offspring between walnut varieties, increases the efficiency of early selection of hybrid offspring, shortens the breeding cycle, and improves breeding efficiency.

The present invention discloses an InDel marker primer for identifying the authenticity of the F1 generation of interspecific hybridization of sisal hemp and its application. According to the resequencing data of sisal parental varieties, the present invention develops an InDel marker primer for identifying the authenticity of the F1 generation of hybridization of sisal hemp '292' and 'Moyin 7', and performs identification at the seedling stage of the hybrid F1 generation. The results show that the offspring that can simultaneously amplify the characteristic bands of the father and the mother are true hybrids, and the lack of any one of the bands or the lack of two bands are regarded as false hybrids, which improves the detection purity of the true hybrids of the F1 generation and also advances the identification time. At the same time, a method for identifying the authenticity of the F1 hybrids of interspecific hybridization of sisal hemp varieties is provided. Compared with the traditional hybrid identification method, it is fast, convenient, accurate and reliable, has a short breeding cycle and low breeding cost, and can also screen the breeding target offspring early, effectively improve the identification efficiency of interspecific hybrids of sisal hemp, and provide a more effective method for quickly identifying the authenticity of the offspring of interspecific hybridization of sisal hemp.

This invention discloses a method for creating a flavorful sweet and sour melon through interspecific hybridization of thick-skinned melon and cucumber. Using the thick-skinned melon inbred line 'Baipicui' as the female parent and the cucumber inbred line 'Jinyou 406' as the male parent, artificial pollination and hybridization are performed to obtain interspecific hybrid offspring. These offspring are then subjected to several generations of self-pollination and selection to obtain the flavorful sweet and sour melon. This invention is the first to utilize interspecific hybridization between high-quality thick-skinned melon and cucumber to create a new high-quality flavorful sweet and sour melon germplasm. This new germplasm has a moderate sweet and sour taste, a well-proportioned fruit shape, and green flesh, and can be directly used for melon variety improvement.

The application provides a specific molecular marker of Malus sieversii non-stinging No.1 non-stinging strain and application thereof, and belongs to the technical field of crop molecular breeding. The application can simply and quickly identify the non-stinging No.1 non-stinging strain in Malus sieversii materials by simultaneously detecting two marker sites through one double-PCR, and using two InDel markers, and meanwhile, the simultaneous detection of the two marker sites can ensure the accuracy, effectiveness and reliability of the detection results by mutual verification and mutual testing, so that the application has great application value in the aspects of distinguishing Malus sieversii varieties and identifying interspecific hybrid seeds, and can provide technical support for innovative large-fruit non-stinging fresh-eating Malus sieversii varieties. In addition, the application can shorten the breeding cycle of non-stinging Malus sieversii and accelerate the breeding process by extracting leaf genomic DNA of the seedling of the breeding material at the seedling stage for double-PCR amplification, and identifying whether it is the non-stinging No.1 non-stinging strain by observing electrophoresis bands.

The invention discloses a method for improving the tobacco interspecific hybridization maturing rate through plant hormone treatment, and belongs to the technical field of crop breeding. The method comprises the following steps: selecting tobacco parents with consistent flowering phases for interspecific hybridization, and applying GA3 to stigmas within 20 minutes before pollination; 1 day after pollination, IAA is used for spraying pistils; 3 days after pollination, 6-BA is sprayed to pistils; cultivated tobacco is used as a female parent in the tobacco parents. According to the method, the pistil of the tobacco female parent is treated by different hormones in three stages before and after pollination, and the three different hormones are combined to take effect and play a role together, so that the aim of improving the tobacco interspecific hybridization setting rate is fulfilled, and the method has important value in the field of tobacco interspecific hybridization breeding.

The invention provides a method for producing tetraploid oysters with different shell colors through interspecific hybridization. The method comprises the following steps: firstly, selecting Fujian oysters and crassostrea gigas with common shell color, golden shell color and black shell color and excellent growth traits as parents, and respectively obtaining triploid and tetraploid individuals through a chemical induction technology; and then carrying out continuous 2-4 generations of population breeding to obtain Fujian oyster and crassostrea gigas tetraploid populations with stable ploidy and different shell color characteristics. Then, the tetraploid populations are hybridized to obtain hybrid tetraploid oysters with different shell colors, and the hybrid tetraploid oysters have three shell colors including a common shell color, a gold shell color and a black shell color. The produced tetraploid oysters have high growth speed, high ploidy stability and high survival rate, and excellent germplasm resources can be provided for the triploid oyster industry. The method not only improves the cultivation benefits of the oysters, but also has wide industrial application prospects. The method combines the technologies of selective breeding, polyploid breeding, cross breeding and the like, breaks through the limitation of a traditional breeding method, has higher breeding efficiency and application potential, is particularly suitable for oyster breeding in coastal areas of China, and promotes the oyster industry to be transformed and upgraded to a high-end consumer market.

The present disclosure relates to eggplant and pepper allotetraploid plants and hybrid allopolyploid plants having desirable traits, such as resistance to an abiotic or biotic stressor, which may be used as rootstock for cultivated varieties. The disclosure further relates to composite eggplants and pepper plants comprising the allopolyploid plants described herein as the rootstock. The disclosure further relates to methods of producing eggplant and pepper allopolyploid plants and hybrid allopolyploid plants.

The present invention relates to the field of biotechnology, and in particular to a method for observing the growth behavior of pollen tubes of macadamia nuts. Specifically, the method comprises the following steps: (1) immersing the pollinated pistil in a fixing solution for fixing; (2) soaking the pistil in an alcohol solution and then washing it; (3) mixing the pistil with a NaOH solution to soften it, washing it, and absorbing the water; (4) soaking the pistil in a phosphate buffer solution; (5) cross-cutting the pistil at the middle position to separate the stigma of the upper half of the pistil from the ovary of the lower half, and then cutting it into two halves longitudinally, and soaking it in a phosphate buffer solution again; (6) staining the pistil with a dye after absorbing the water, and microscopically examining it. The fluorescence microscopy method of the present invention greatly weakens the phenomenon of luminescence of the pistil's own tissue, and it is easy to obtain a high-definition image of the pollen tube growing in the pistil. The method has an ideal presentation effect on the growth behavior of the pollen tube, and can be used for the hybridization affinity evaluation between macadamia nut varieties, and has high practical application value.

The invention relates to the technical field of crop breeding, and particularly discloses a water melon and towel gourd crossbreeding method which comprises the following steps: S1, parent selection and cultivation: taking a high-yield tender variety of water melons and a variety of towel gourds with strong disease resistance and obvious furrows as parents, and performing parent cultivation on the parents; s2, parent hybridization: performing hybrid pollination by taking the water melon as a female parent and the towel gourd as a male parent; or performing cross pollination by taking the towel gourd as a female parent and the water melon as a male parent; or carrying out selfing pollination on the watermelons or the towel gourds; s3, seed cultivation: S3-1, management after pollination; (S3-2) reserving hybrid fruits; and S3-3, collecting the hybridized and self-propagated seeds. According to the method, the feasibility of interspecific hybridization of luffa cylindrica (common luffa) and luffa cylindrica (luffa with ridges) is broken through, the limitation of reproductive isolation is broken through, two different hybrid F1-generation seeds are obtained, the excellent hereditary characteristics, such as high yield, disease resistance, stress resistance and high quality, of the luffa cylindrica and the luffa cylindrica are integrated, and a new variety with comprehensive advantages is cultivated.

The invention discloses a primer pair for identifying the authenticity of a hybrid of a lycium ruthenicum filial generation as well as application and a use method of the primer pair, and belongs to the technical field of crossbreeding of lycium ruthenicum. The primer pairs comprise an LR0018 primer pair, an LR0037 primer pair, an LR0043 primer pair, an LR0112 primer pair and an LR0140 primer pair. The method for identifying the hybrid of the lycium ruthenicum based on the five pairs of SSR primers is established by combining an SSR marker with a capillary electrophoresis technology, and a new technical approach is provided for breeding a new variety of the lycium ruthenicum. By amplifying the specific expression SSR marker of the F1 generation, hybrid identification is carried out on the filial generation, the identification process is simple, convenient and rapid, the cost is low, the identification result is stable and reliable, and the research result has important significance on early selection of the authenticity of the hybrid of the filial generation among the lycium ruthenicum varieties and improvement of the breeding efficiency.

The invention relates to the field of plant conservation and breeding optimization, in particular to a hybrid embryo rescue method and a method for creating a large number of interspecific hybrid seedlings of Populus nigra by using the hybrid embryo rescue method. The method comprises the following steps: establishing an embryo rescue system, namely observing a separation layer between infructescence and branches after pollination for about 2 months, cutting part of infructescence to develop the embryo rescue system, washing capsules with running water for 120 minutes, lightly wiping peels with 75% alcohol for about 2 minutes, washing the capsules with sterile water for 5-6 times in a super clean bench, lightly absorbing moisture on the surfaces of the capsules with sterile filter paper, and taking out the capsules; the method comprises the following steps of: cutting a capsule by using a sterile scalpel, thoroughly removing floc, carefully clamping seeds by using sterile tweezers, putting the seeds on a 1 / 2MS culture medium containing auxin analogues for embryo rescue, and screening out an optimal culture medium which is 1 / 2MS + 0.06 mg / L naphthylacetic acid (NAA) + 0.08 mg / L indolebutyric acid (IBA) and has the pH value of 5.8-6.0 by adopting a culture dish germination method and taking 1 / 2 MS as the culture medium.

The application discloses a high-pungent pepper variety breeding analysis method, and relates to the technical field of pepper variety breeding, and comprises the following steps: S1: interspecific hybridization is carried out on Chinese pepper and annual pepper to breed new pepper varieties with high pungency; S2: observation results are analyzed, pepper capsaicin is determined, and a pepper strain with optimal disease and pest resistance and capsaicin is screened out; the high-pungent pepper variety breeding analysis method tests the disease and pest resistance of the pepper by releasing diseases and pests in the planting experimental area during the cultivation of the pepper, collects the pepper during the testing of the disease and pest resistance of the pepper, records the observation results of the disease and pest resistance of the pepper, analyzes the observation results, and screens out high-probability high-quality combinations in the pepper matching condition, so that the disease and pest resistance of the pepper matching combinations analyzed by the pepper variety breeding is good.