7 results about "Interspecific hybridization" patented technology

This invention discloses PCR primers, interspecific hybridization methods, and applications for identifying interspecific hybrid offspring of swimming crabs, belonging to the field of aquatic animal genetic engineering technology. The PCR primers of this invention include an upstream primer as shown in SEQ ID NO.1 and a downstream primer as shown in SEQ ID NO.2. When using these PCR primers for PCR amplification, a 786 bp band indicates *Portunus trituberculatus*; a 632 bp band indicates *Portunus cerana*; and a 963 bp band indicates *Portunus rubrum*. The hybrid offspring simultaneously exhibit two specific amplification bands, thus achieving rapid and accurate identification of the hybrid offspring. This invention establishes for the first time a method for identifying hybrid offspring based on PCR amplification fragment length polymorphism, which has advantages such as simple operation, low cost, high accuracy, and suitability for large-scale detection. It can be applied to the fields of swimming crab germplasm innovation, new variety breeding, and genetic improvement.

The application discloses a high-pungent pepper variety breeding analysis method, and relates to the technical field of pepper variety breeding, and comprises the following steps: S1: interspecific hybridization is carried out on Chinese pepper and annual pepper to breed new pepper varieties with high pungency; S2: observation results are analyzed, pepper capsaicin is determined, and a pepper strain with optimal disease and pest resistance and capsaicin is screened out; the high-pungent pepper variety breeding analysis method tests the disease and pest resistance of the pepper by releasing diseases and pests in the planting experimental area during the cultivation of the pepper, collects the pepper during the testing of the disease and pest resistance of the pepper, records the observation results of the disease and pest resistance of the pepper, analyzes the observation results, and screens out high-probability high-quality combinations in the pepper matching condition, so that the disease and pest resistance of the pepper matching combinations analyzed by the pepper variety breeding is good.

Provided herein is computational tool design for analyzing allele-specific DNA methylation patterns in in-crosses of inter-strain and inter-specific crosses of organisms. The tool can be incorporated into a system for analyzing allele-specific DNA methylation patterns. allele-specific DNA methylation patterns in in-crosses of inter-strain and inter-specific crosses of organisms is used for analyzing inheritance of epigenetic patterns including for identifying Mendelian and non-Mendelian patterns of epigenetic inheritance. The systems described herein can be used in methods of screening a subject for susceptibility to a disease or condition, and in methods of generating a prognosis of a human subject's susceptibility to a disease or condition.

The present application firstly obtains the diploid Fujian oyster common, shell gold and shell black strains with fast growth and pure shell color through population breeding. Secondly, the tetraploid Fujian oyster common, shell gold and shell black strains with high and stable tetraploid rate and stable shell color inheritance are obtained through ploidy breeding. Then, the tetraploid populations of long oyster 'Haida 1', 'Haida 2' and 'Haida 3' are obtained by using ploidy breeding technology with the pure shell color, shell type and large size of the individuals as parents. Finally, the fast-growing and high-temperature-resistant hybrid triploid oyster new strains with different shell colors are obtained through interspecific hybridization. The hybrid triploid oyster new strains obtained in the present application maintain the fast growth and strong stress resistance of long oyster, and have the excellent high-temperature-resistant traits of Fujian oyster. In addition, the shell color of the gold and black hybrid triploid oyster new strains is pure and the color is beautiful. The hybrid triploid oyster new strains obtained in the present application expand the germplasm resources of the shell color strains of the cultured oyster, provide important germplasm resources for the high-quality development and transformation and upgrading of the oyster industry to the high-end consumer market, and have a broad industrial application prospect.

The application belongs to the technical field of biological breeding, and specifically discloses a branch fungus transcription factor SycMYB6 and application thereof in regulating drought stress tolerance of plants, and specifically provides application of overexpressed transcription factor SycMYB6 or a protein coded by the transcription factor SycMYB6 in breeding drought-resistant crop germplasm, and a nucleotide sequence of the transcription factor SycMYB6 is shown as SEQ ID NO. 1. The application can improve drought resistance of crops and breed excellent germplasm resources by overexpressing the transcription factor SycMYB6 gene in crops through genetic engineering, and compared with traditional breeding, the technology has short cycle, clear target, is not limited by intergeneric cross, and greatly improves the efficiency of breeding drought-resistant crop germplasm.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

This invention discloses a method for interspecific hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, comprising: selecting mature, live male *Pterocarya spp.*, cleaning and drying them, placing them in seawater, continuously aerating and artificially circulating the water to induce natural sperm release; dissecting mature female *Pterocarya martensii* to extract gonads, collecting mature eggs, cleaning them, and allowing them to settle to obtain an egg mixture; adding ammonia to the egg mixture to activate the eggs; adding the activated egg mixture to a sperm-containing water body for fertilization in the dark; after successful fertilization, removing the male *Pterocarya spp.* and transferring the fertilized eggs to a seedling pond; and cultivating the fertilized eggs in seawater to develop into D-shaped larvae. This invention is the first of its kind for distant hybridization and seedling cultivation of *Pterocarya spp.* and *Pterocarya martensii*, breaking through the reproductive isolation between the two species, achieving stable and efficient hybridization and fertilization. The process is simple and highly stable, and can cultivate new hybrid pearl oyster seedlings that combine the excellent traits of both parents. It has significant implications and promotional value for the innovation of marine pearl oyster germplasm and industrial upgrading.