Cultivation Method of Cucumber Introgression Line Material Resistant to Meloidogyne incognita

A technology of root-knot nematode incognita and its breeding method, which is applied in the field of cultivation of cucumber introgression line materials resistant to root-knot nematode incognita, can solve problems such as infertility of hybrid offspring and interspecific hybridization incompatibility, and achieve the effect of broadening the genetic basis

Inactive Publication Date: 2011-11-30
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Among the closely related species of cultivated cucumber, there are many genes that are useful for cuc

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  • Cultivation Method of Cucumber Introgression Line Material Resistant to Meloidogyne incognita
  • Cultivation Method of Cucumber Introgression Line Material Resistant to Meloidogyne incognita
  • Cultivation Method of Cucumber Introgression Line Material Resistant to Meloidogyne incognita

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Embodiment 1

[0047] Cucumber root-knot nematode is a worldwide disease that seriously affects the yield and quality of cucumber. The global annual loss is as high as 100 billion U.S. dollars, and the annual loss rate is about 10%. [4] At present, no germplasm resources resistant to root-knot nematode incognita have been found in cucumber cultivars. The present invention uses cucumber interspecific hybrid introgression lines as materials, adopts root-knot nematode ovum inoculation method, and introgression line materials of different strains Carry out resistance identification, the result finds that the material of numbering 10299F-5 is resistant to root-knot nematode incognita, and the disease index is 2.0, and we have obtained the cucumber material of resistance to root-knot nematode incognita by means of the method of the present invention, and the cultivation technology roadmap is shown in figure 1 , the specific process is as follows:

[0048] 1. Acquisition of cucumber introgression l...

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Abstract

The invention belongs to the field of seed breeding in biotechnology, and relates to a method for cultivating cucumis sativus L introgression line material for resisting meloidogyne incognita. The method comprises the following steps of: performing the interspecific hybridization of wild species and cultivated species, doubling chromosomes to obtain new amphidiploid species, and performing backcross and selfing to obtain an interspecific introgression line; identifying the cucumis sativus L introgression line by a method of inoculating artificial seedling stage spawn liquid to obtain the cucumis sativus L interspecific hybridization introgression line material for resisting meloidogyne incognita, wherein an identification result indicates that disease indexes of the introgression line 10299F-5 are 2.0, and the introgression line 10299F-5 has disease-resistant expression. The cultivating cucumis sativus L interspecific hybridization introgression line material for resisting meloidogyneincognita is high in an economical character, and can be used as a breeding material directly, so the blank of cucumis sativus L resources for resisting meloidogyne incognita is filled, a reference is provided for the inoculation identification of other plants, and useful genetic materials are provided for improvement on cucumis sativus L species, the transfer of disease-resistant genes, gene positioning, molecular marks, gene cloning and the like in future.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding and relates to a method for cultivating cucumber introgression line materials resistant to root-knot nematode incognita. Background technique [0002] Meloidogyne incognita is the main pathogenic nematode harmful to cucumber (Cucumis sativus L.) production [1、2] , the global annual losses are as high as more than 100 billion U.S. dollars [3] , the annual loss rate is about 10% [4] , has become one of the serious obstacles affecting cucumber production. No germplasm resources resistant to Meloidogyne incognita have been found in cucumber cultivars [5、6、7] . Due to the very narrow genetic base of cucumber, the variation rate is only 3-8% [8] , Therefore, it is difficult to obtain cucumber varieties resistant to M. incognita through intraspecific variation. Many studies have shown that melons belong to some wild relatives, such as C.metuliferus E.Meyer ex Naudin, C.anguria L., C.ficifolius...

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Application Information

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IPC IPC(8): A01H1/02A01G7/06
Inventor 陈劲枫张燕霞叶德友钱春桃娄群峰程春燕
Owner NANJING AGRICULTURAL UNIVERSITY
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