Culture medium for in-vitro directional screening of peanut high oil body and screening method

Abstract

The invention discloses a culture medium for in-vitro directional screening of a peanut high oil body and a screening method, and belongs to the technical field of peanut breeding. The culture mediumfor in-vitro directional screening of the peanut high oil body comprises a somatic embryo germination and screening culture medium, a seedling formation and screening culture medium and a recovery culture medium; the somatic embryo germination and screening culture medium takes an MS culture medium as a basic culture medium, and 3-5mmol / L HYP and 3-5mg / L BAP are added; the seedling formation and screening culture medium takes the MS culture medium as a basic culture medium, and 7-9mmol / L HYP and 1-3mg / L BAP are added; and the recovery culture medium takes the MS culture medium as a basic culture medium, and 1-3mg / L BAP is added. The culture medium is adopted to screen the peanut high oil body, the screening efficiency can be improved, and the seedling hardening time of screened plants in the later period can be effectively shortened.

Description

[0001] This application is a divisional application of the patent application of "Patent Application No. 201810125227.7, application date 2018.02.08, application name is an in vitro targeted screening method for peanut high oil bodies". Technical field [0002] The invention belongs to the technical field of flowering species, and in particular relates to a culture medium and a screening method for in vitro targeted screening of peanut high oil bodies. Background technique [0003] Peanuts are an important oil crop in my country and occupy an important position in the national economy. More than 50% of peanuts produced in my country are used for oil extraction. Generally, high-oil varieties or high-oil materials with an oil content of more than 50% are used. Each increase in oil content by 1% can increase net profit by 7%. Therefore, new varieties of high-oil peanuts will be cultivated. It has become one of the main breeding goals of breeders. However, peanuts lack resources of hi...

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Problems solved by technology

Jiang Defeng et al. (Journal of Nuclear Agriculture, 2017, 31, 1678-1683) used 60 Co-γ ray irradiation (irradiation dose 150Gy) peanut variety Luhua No. 11, a new line with variation in pod traits and a crude fat content of 54.3%, the results of the paper show that the mutation can increase the oil content of peanuts, but it cannot be oriented Obtained, can only be obtained randomly, and some high-oil mutants are easy to be eliminated because of low production

Li Guan et al. (Agricultural Biotechnology, May 25, 2017, 33(5): 766-774) used in vitro mutagenesis to cultivate a new peanut variety Yuhua No. 4, and disclosed a method for cultivating a new peanut variety. Use pingyangmycin-containing medium for mutagenesis, and then use HYP for directional screening, but the method needs to be cultivated in the acclimatization room for 3 weeks before transplanting to the field. During the 3-week period in the acclimatization room, the seedlings grow slowly and are easy to be infected. It consumes manpower and material resources and takes up laboratory space. At the same time, the cultivation process in the acclimatization room affects the growth

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