Pretreatment method for cryopreservation of soybean pollen
A technology of ultra-low temperature preservation and pretreatment, which is applied in the fields of plant preservation, botanical equipment and methods, and legume cultivation, etc. It can solve problems such as the influence of pollen activity, break through time and space constraints, expand the scope of application, and broaden genetics. basic effect
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[0034] Embodiment 1, establishment of soybean pollen cryopreservation pretreatment method
[0035] 1. Materials and methods
[0036] 1. Soybean varieties
[0037] Heihe No. 43, approval number: Heixindou 2007011; breeding unit: Heihe Branch of Heilongjiang Academy of Agricultural Sciences. The growth period of spring sowing in Heihe area is 113 days, with purple flowers, long leaves and gray hairs.
[0038] Jinyuan No. 55, approval number: National Examination Bean 2013001; breeding unit: Heihe Branch of Heilongjiang Academy of Agricultural Sciences. The growth period of spring sowing in Heihe area is 118 days, with white flowers, long leaves and gray hairs.
[0039] 2. Field planting conditions
[0040] The field experiment was carried out at the Heihe Branch of the Heilongjiang Academy of Agricultural Sciences (50°15′ north latitude, 127°27′ east longitude, 168.5m above sea level). The seeds were sown on May 9, 2018 and May 11, 2019 respectively, with a row length of 1.5...
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