Pretreatment method for cryopreservation of soybean pollen

A technology of ultra-low temperature preservation and pretreatment, which is applied in the fields of plant preservation, botanical equipment and methods, and legume cultivation, etc. It can solve problems such as the influence of pollen activity, break through time and space constraints, expand the scope of application, and broaden genetics. basic effect

Inactive Publication Date: 2021-08-20
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Previous studies have shown that the drying method is more suitable for the pretreatment of pollen to be preserved, but the pretreatment methods of pollen of different plant species before low temperature storage are quite different, and the thawing method also has a certain impact on pollen activity
For soybean, an important economic plant, there is no report on the pretreatment method of cryopreservation for its pollen

Method used

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  • Pretreatment method for cryopreservation of soybean pollen
  • Pretreatment method for cryopreservation of soybean pollen
  • Pretreatment method for cryopreservation of soybean pollen

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Experimental program
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Embodiment 1

[0034] Embodiment 1, establishment of soybean pollen cryopreservation pretreatment method

[0035] 1. Materials and methods

[0036] 1. Soybean varieties

[0037] Heihe No. 43, approval number: Heixindou 2007011; breeding unit: Heihe Branch of Heilongjiang Academy of Agricultural Sciences. The growth period of spring sowing in Heihe area is 113 days, with purple flowers, long leaves and gray hairs.

[0038] Jinyuan No. 55, approval number: National Examination Bean 2013001; breeding unit: Heihe Branch of Heilongjiang Academy of Agricultural Sciences. The growth period of spring sowing in Heihe area is 118 days, with white flowers, long leaves and gray hairs.

[0039] 2. Field planting conditions

[0040] The field experiment was carried out at the Heihe Branch of the Heilongjiang Academy of Agricultural Sciences (50°15′ north latitude, 127°27′ east longitude, 168.5m above sea level). The seeds were sown on May 9, 2018 and May 11, 2019 respectively, with a row length of 1.5...

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Abstract

The invention discloses a pretreatment method for cryopreservation of soybean pollen. The invention provides a method for long-term cryopreservation of soybean pollen through pretreatment. The method can keep the activity of the soybean pollen in vitro for a long time, and the pollen preserved at the ultralow temperature is successfully applied to hybridization to obtain hybrid seeds. According to the invention, the time and space limitation of soybean hybridization can be broken through, and technical support is provided for solving the problems of flowering asynchronism and cross-regional breeding of soybean hybridization breeding; the method has important significance in expanding the application range of soybean variety breeding parents and widening the genetic basis of soybean varieties; and technical support can be provided for pollen culture and genetic transformation.

Description

technical field [0001] The invention relates to a method for maintaining the activity of plant pollen, in particular to a pretreatment method for cryopreservation of soybean pollen. Background technique [0002] Sexual hybridization is the main way of soybean breeding, and hybridization needs the parents to meet at flowering stage to proceed smoothly. Because soybeans are sensitive to light and temperature, the flowering period of the varieties differs greatly, and the varieties that cannot meet at the flowering period cannot be directly hybridized. In addition, due to the short lifespan of pollen under natural conditions, soybean hybridization can only be carried out during the co-flowering period of nearby planting parents, resulting in a short time suitable for hybridization and low efficiency. At present, soybean hybrid breeding mostly uses varieties with similar geographical origins and similar flowering periods as parents, and hybridization can only be carried out in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00A01G22/40A01H1/02
CPCA01H1/02A01N3/00A01G22/40
Inventor 韩天富梁昕贾鸿昌鹿文成孙石闫洪睿张金梅宋雯雯
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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