The invention mainly relates to a method for regenerating a
plant from camellia
callus. The method has the following steps: stripping off the inner and outer seed coats of a camellia fruit seed, and inoculating the seed to a 1 / 2MS culture medium; when a sterile
seedling grows above 3 cm, inoculating the soft leaf of the young
plant to a
callus induction culture medium which is MS + 0.5mg.L 6-BA+1.0mg.L 2,4-D; when the
callus grows to get a
diameter about 1 cm, shifting the callus to a callus differentiation culture medium which is MS+mg.L 6-BA20+0.1mg.L I BA+ mg.L KT0.1; when an indefinite
bud grows to 0.5 cm, carrying out the separation and inoculating to a strong
bud culture medium which is MS + 0.2mg.L 6-BA+0.05mg.L NAA; and when a
bud stick grows to 4 to 5 cm,
cutting off the basal of the bud stick, immersing the basal of the bud stick in 1,000 g.L I BA, and then inoculating to a MW + 0.2mg.L I BA+0.2mg.L NAA culture medium. The method has the advantages that: the method has a simple culture medium
recipe, a simple and convenient operating process, short culture time, high regeneration frequency and a high propagation expansion coefficient, and facilitates the large-scale production of rare camellia plants and the realization of the genetic transformation of exogenous genes.