1514 results about "Genetics transformation" patented technology

The present invention relates to the fields of genetic engineering and plant genetic breeding, and specifically to the application of wheat TaPND gene. In the present invention, by taking gene TaPND as a target, designing a sgRNA sequence based on CRISPR / Cas9, connecting a DNA fragment containing the encoding sgRNA sequence to a vector carrying CRISPR / Cas, a plant binary expression vector capable of knocking out the TaPND gene in wheat is constructed; by genetically transforming wheat, offspring with TaPND gene knockout are obtained, and wheat mutants with early flowering time are obtained. Experiments have confirmed that transgenic wheat with TaPND gene knockout achieves the advancement of wheat flowering time. The technical scheme of the present invention provides a practical method for realizing rapid wheat breeding using genetic engineering technology, which has important breeding application value and broad market application prospects.

The invention discloses application of a wheat WRKY transcription factor gene TaWRKY1, and belongs to the technical field of gene engineering. A nucleotide sequence of an open reading frame of the gene TaWRKY1 is shown as SEQ ID NO: 1, and the gene TaWRKY1 is infected by stripe rust to induce expression in interaction of wheat and stripe rust, and plays a negative regulation role in wheat stripe rust resistance. A TaWRKY1 gene editing mutant plant is created by using an agrobacterium tumefaciens-mediated genetic transformation method, the sporulation quantity on wheat leaves of the gene editing mutant is obviously reduced, and the allergic necrosis is increased, which indicates that the edited gene TaWRKY1 can improve the resistance of wheat to stripe rust. The invention provides a disease-resistant germplasm resource for the cultivation of the stripe rust-resistant wheat variety.

The invention discloses an application of a peony PoAIL6 gene and an encoding protein thereof, and belongs to the technical field of biology. According to the invention, the peony PoAIL6 gene is cloned from a peony cultivar 'Paeonia ostii', and bioinformatics analysis is carried out. According to the invention, the peony PoAIL6 gene is transferred into arabidopsis thaliana, and phenotypic contrast finds that the number of lateral roots of the transgenic arabidopsis thaliana is significantly increased, which indicates that the overexpression of the peony PoAIL6 gene can promote the development of the root system of the arabidopsis thaliana. Arabidopsis thaliana roots are used for inducing calluses and carrying out callus differentiation, and it is found that the overexpressed peony PoAIL6 gene inhibits formation of the calluses and promotes the calluses to be differentiated into buds at the same time, which indicates that the peony PoAIL6 gene can regulate formation of arabidopsis thaliana somatic embryos. The invention provides a certain theoretical basis for establishing a complete plant regeneration and genetic transformation system of peony.

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention provides application of an ECT2 gene in regulating and controlling development of secondary xylem of a poplar. Transgenic poplar with overexpressed ECT2 genes is obtained through genetic transformation, it is found that overexpressed ECT2 genes can increase the width of secondary xylem of the poplar, and the nucleotide sequence of the ECT2 genes is shown as SEQ ID NO: 1. The ECT2 gene has a regulation effect on the development of the secondary xylem of the poplar.

The invention belongs to the technical field of fungal gene engineering, and discloses a shiitake mushroom ABC transporter gene LeABC2 and application thereof in regulation and control of shiitake mushroom heat resistance. Whole genome association analysis is carried out based on heat resistance phenotype data of 133 shiitake mushroom strains, a candidate gene LeABC2 for regulating heat resistance is obtained, a LeABC2 gene RNA interference vector is constructed, the gene function is researched through an agrobacterium tumefaciens-mediated genetic transformation method, and the result shows that the LeABC2 gene negatively regulates the heat resistance of shiitake mushroom hyphae, the heat resistance of the hyphae can be improved by interfering the expression of the gene, and the gene has a good application prospect. A new solution thought is provided for breaking through the problem that the yield and quality are influenced by high temperature in the mushroom industry.

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention is applicable to the technical field of biotechnology and genetic engineering, and provides an agrobacterium rhizogenes mediated genetic transformation method for hairy roots of blueberries. According to the method, a blueberry perennial stem segment is used as a receptor material for infection, and rapid genetic transformation of the blueberry stem segment is realized through an agrobacterium tumefaciens-mediated method. The method ingeniously bypasses the lengthy process of differentiating the explants to form somatic embryos, and compared with a traditional leaf disc method genetic transformation system, the method has the advantage that the genetic transformation period is remarkably shortened. And the method can be used for mass infection. Even in a non-sterile environment, the method can realize high-efficiency transformation of the gene, and shows the characteristics of simplicity and high efficiency. Therefore, the invention not only provides a new way for genetic transformation of the blueberries, but also provides valuable reference for genetic transformation research and gene function research of the blueberries.

Disclosed is an innovative method for genetic transformation through injection of Agrobacterium containing plasmid DNA expressing PLETHORAs (PLT5) or / and WOUNDINDUCED DEDIFFERENTIATION 1 (WIND1) in multiple plant species including Snapdragon and tomato. In addition, this plasmid has also been tested that it can significantly improve the genetic transformation method is not genotype dependent. Genetic transformation can be achieved by promoting de-novo regeneration of shoots in adult plants, which will of significant impact on some perennial plant species such as citrus and blue berry etc. The transgenic de-novo shoot regenerated from adult plants can by-pass the juvenile phase of plants regenerated through the tissue culture method. Expression of PLT5, WIND1, or both can also greatly improve plant regeneration and transgenic transformation through in-vitro tissue culture in recalcitrant plant species.

The invention discloses an efficient agrobacterium tumefaciens-mediated euphorbia hirta genetic transformation method, and belongs to the technical field of plant genetic engineering. The method mainly comprises the following steps: 1) activation culture of agrobacterium tumefaciens; 2) seed disinfection and material selection; (3) inducing calluses; 4) preparation of an infection liquid: culturing agrobacterium in a YEB liquid culture medium, adding the agrobacterium into an MS liquid culture medium, adjusting the bacterial liquid until OD600 is 0.3-0.9, then adding 100 [mu] mol / L acetosyringone and 100 [mu] mol / L MgCl2, and shaking uniformly; the method comprises the following steps: (1) carrying out primary culture, (2) carrying out secondary culture, (3) carrying out secondary culture, (4) carrying out secondary culture, (5) carrying out infection, (6) carrying out co-culture, (7) carrying out cleaning and recovery culture, (8) carrying out screening differentiation and rooting induction, and (9) carrying out plant regeneration and authentication.Through the steps, a euphorbia hirta genetic transformation system can be successfully constructed, and the transformation rate of a positive plant obtained through transformation reaches 76.19%.

The invention discloses application of an Afa04Dg76000 gene in resistance of an oat herbicide. The Afa04Dg76000 gene is knocked down to reduce the resistance of the oat herbicide, and the Afa04Dg76000 gene is overexpressed to improve the resistance of the oat herbicide; the nucleotide sequence of the Afa04Dg76000 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the Afa04Dg76000 gene is as shown in SEQ ID NO. 2. According to the application of the Afa04Dg76000 gene in the herbicide resistance of the oats, a gene knock-down and overexpression strain of the Afa04Dg76000 is constructed through a TRV virus-mediated gene silencing (VIGS) system and a genetic transformation system of the oats, the herbicide resistance function of the Afa04Dg76000 is proved, and a new target is provided for germplasm improvement of cultivated oats.

The invention relates to the technical field of plant tissue culture, in particular to a Rhododendron pulchrum tissue culture method based on callus regeneration. According to the method, the tender cotyledons of aseptic seedlings are taken as explants, and an efficient Rhododendron pulchrum tissue culture and rapid propagation technical system is established through induction of calluses, subculture multiplication of the calluses, differentiation of adventitious buds, bud strengthening and rooting culture, so that complete tissue culture progeny plants are formed. The induction rate of the callus tissue of the system is 100.0%, the proliferation multiple of the callus tissue in a subculture cycle reaches 5.2 times, 28.5 adventitious buds can be differentiated from a callus tissue block with the size of about 0.5 mm * 0.5 mm * 0.5 mm, the rooting rate reaches 95.8%, the system is not limited by factors such as seasons, climate and regions, and efficient and rapid propagation can be achieved all the year round. Meanwhile, the establishment of the system also lays a technical foundation for genetic transformation improvement, mutant screening breeding and the like of the Rhododendron pulchrum based on the callus.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, Ilyonectria fungus hyphae are prepared, Ilyonectria fungi are cultured on a PDA solid culture medium for 14 d, then the Ilyonectria fungi are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture for 4 d and filtered, filtrate is centrifuged for 10 min at the speed of 10000 rpm, and spores are collected; diluting the concentration of spores to 107 pieces / mL, inoculating the spores into a YEPD liquid culture medium, carrying out shake-flask culture for 2 days, and filtering and collecting hyphae; and S12, preparing the Ilyonectria fungus protoplast, namely adding the hypha prepared in the step S11 into the enzymatic hydrolysate, performing enzymolysis in a shaker at the temperature of 30 DEG C at the rotating speed of 150-200rpm for 3-5 hours, filtering, centrifuging filtrate at the temperature of 4 DEG C, discarding supernate, adding 0.7 M NaCl for centrifuging, and discarding the supernate, thereby obtaining the Ilyonectria fungus protoplast. The genetic transformation constructed by the invention can be used for exploring the specific action and mechanism of a specific pathogenic gene in the pathogenic process of Ilyonectria fungi through gene knockout or overexpression experiments.

The invention belongs to the technical field of genetic engineering, and particularly relates to an AvMYB1 protein and application thereof in improving salt tolerance of plants. The invention provides an AvMYB1 protein, wherein the amino acid sequence of the AvMYB1 protein is as shown in SEQ ID NO. 2. The AvMYB1 protein disclosed by the invention can be used for regulating the growth and development of plants and improving the stress resistance of the plants; according to the AvMYB1 protein, Na < + > is discharged by activating an SOS pathway, so that the Na < + > content in a plant body is reduced, and the salt tolerance of the plant is improved. The AvMYB1 gene is constructed into a plant expression vector, genetic transformation is carried out through an agrobacterium tumefaciens-mediated genetic transformation method, new germplasm with the salt-tolerant characteristic can be effectively obtained, and the method has important significance for developing salt-tolerant crop breeding.

The invention relates to the technical field of gene engineering, in particular to a soybean disease-resistant RING-H2 type zinc finger protein gene GmRHF1 and application thereof.According to the technology, the GmRHF1 genes are cloned in soybean varieties Xu bean 14 and Shangshan black beans respectively, and then the expression quantity difference of the genes in different tissues and the expression quantity change trend of the genes under SMV treatment are analyzed through fluorescent quantitative PCR; meanwhile, the anti-SMV function of the GmRHF1 is verified in effective plant silencing and transgenic overexpression by adopting a virus-induced gene transient silencing technology and a soybean stable genetic transformation experiment. Finally, the interaction protein of the GmRHF1 is screened and verified through yeast cDNA library screening, yeast point verification and luciferase complementary test screening, and the biological significance of interaction of the GmRHF1 and the GmClpP6 on soybean SMV resistance is defined. In conclusion, the result of the invention can provide an effective gene resource for cultivating a new variety (line) of soybean with high SMV resistance.

The invention belongs to the technical field of medical biology, and particularly discloses a mycoplasma hyopneumoniae recombinant antigen, an expression vector, a recombinant bacterium and application. The invention provides a construction method of a mycoplasma hyopneumoniae recombinant antigen, which is characterized in that a mycoplasma hyopneumoniae P97 protein R1 region is modified to construct the mycoplasma hyopneumoniae recombinant antigen as shown in SEQ ID No.1. The mycoplasma hyopneumoniae recombinant antigen is cloned into an expression vector, and a recombinant engineering bacterium is obtained by using a genetic transformation means. The recombinant engineering bacterium can stably express the recombinant antigen of the mycoplasma hyopneumoniae, can be prepared into an oral living bacterium preparation, has the characteristics of safety, effectiveness, rapidness and the like, and particularly can prevent infection of the mycoplasma hyopneumoniae to pigs without an immune response process.

The invention discloses a rhodosporidium toruloides strain with high homologous recombination efficiency as well as a construction method and application thereof, and belongs to the field of oil-producing yeast engineering bacteria. According to the rhodosporidium toruloides strain, the rhodosporidium toruloides strain NP11-SaCas9-1 is used as an original strain, and an RAD52 gene is over-expressed. The modified yeast engineering bacterium provided by the invention can realize high homologous recombination through CRISPR system mediation, the homologous recombination efficiency reaches 90-100%, a multi-gene editing platform can be constructed on the basis, and the modified yeast engineering bacterium can be applied to the fields of synthetic biology, metabolic engineering, genetic engineering and the like, so that the modified yeast engineering bacterium is simpler, quicker, more convenient and more accurate in the genetic modification process. And the homologous recombination activity of the rhodosporidium toruloides can be improved by expressing the homologous recombination related protein Rad52, and the genetic engineering strategy can be used for modifying other unconventional yeasts.

The invention discloses a method for verifying CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) or Cas9 carrier activity and gene functions in muskmelon hairy roots, which comprises the following steps: by using cotyledons of muskmelon seeds germinating for two days as explants, infecting by using agrobacterium rhizogenes K599, co-culturing, inducing the hairy roots, and taking green fluorescent protein eGFP as a selection marker of transgenic hairy roots, so as to verify CRISPR or Cas9 carrier activity and gene functions in muskmelon hairy roots. And hairy roots for converting the CRISPR / Cas9 vector can be obtained in about one month. By utilizing the transformation material, availability of gene editing target sites of multiple genes is analyzed, it is found that increase of the copy number of sgRNA expression units has a promoting effect on gene editing efficiency, and it is found that after CmRHL1 is edited, muskmelon root hair cannot be formed. Therefore, the invention provides a convenient and fast way for verifying the activity of the CRISPR / Cas9 vector and researching the function of the gene in the root development process, and is a beneficial supplement for a stable genetic transformation method of the muskmelon.

The invention discloses a method for improving the inductivity and genetic transformation efficiency of hairy roots of toona ciliata. According to the method, agrobacterium rhizogenes carrying RUBY-TcGRF8-GIF2 chimeric protein are injected into stems and petioles of aseptic seedlings of the toona ciliata, and the TcGRF8-GIF2 chimeric gene is over-expressed, so that the induction efficiency and the genetic transformation efficiency of the toona ciliata hairy roots are remarkably improved, and the transformation period of the toona ciliata hairy roots is remarkably shortened. Specifically, after overexpression of the TcGRF8-GIF2 gene, the induction time of the hairy roots is shortened from 70 days of a RUBY-XbaI no-load plasmid control group to 25 days, the induction rate and conversion rate of the hairy roots mediated by agrobacterium tumefaciens are improved, the operation efficiency of genetic transformation of the toona ciliata is effectively improved, and the method has important significance for promoting genetic improvement of the toona ciliata.

The invention discloses a watermelon transgenic screening expression cassette and application thereof, and belongs to the technical field of biology. According to the invention, an expression cassette of a TagRFP gene driven by a d35s promoter is constructed on a pCambia1300 vector, and a recombinant vector is obtained. A recombinant vector is introduced into watermelon cells by utilizing an agrobacterium-mediated genetic transformation technology, and a red fluorescent protein TagRFP gene is integrated into a watermelon genome, so that transgenic watermelons can be screened and identified by taking red fluorescence as an identifier in the whole growth period of watermelon genetic transformation. Compared with a traditional screening method, the screening method has the advantages that the screening process is greatly simplified, and the transgenic watermelon and the non-transgenic watermelon are clearly distinguished, so that the genetic transformation efficiency of the watermelon is remarkably improved.

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention relates to the technical field of plant genetic engineering, in particular to a gene of leymus secalinus participating in adversity stress and application of the gene. The gene LsHSFA participating in adversity stress in leymus secalinus is found for the first time, the nucleotide sequence of the gene LsHSFA is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO.2. Through cloning and identification of the adversity stress participating gene of the leymus secalinus, expression analysis of the gene, genetic transformation of the gene and other means, the function of the gene is analyzed and verified, and the result shows that the adversity stress participating gene of the leymus secalinus has important influence on promotion of upper development of the leymus secalinus and improvement of heat resistance, salt resistance, drought resistance and other stress resistance; the stress-resistant gene of the leymus secalinus is utilized to positively regulate and control the abiotic stress function, so that the leymus secalinus can cope with various stress adversities better, the leymus secalinus can adapt to more complex and harsh ecological environments, degenerated and desertified grassland is improved, and potential application prospects are provided for improved variety breeding and excellent population construction of the leymus secalinus in the future.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention belongs to the technical field of genetic engineering, and particularly relates to an agrobacterium rhizogenes-mediated visualized genetic transformation method for cucurbitaceae plants, which comprises the step of transfecting the cucurbitaceae plants by using agrobacterium rhizogenes containing recombinant plasmids, and the recombinant plasmids are expression cassettes containing Ruby reporter genes. A visual agrobacterium rhizogenes-mediated transformation system of the cucurbitaceae plants is established by utilizing the RUBY gene, professional equipment or chemical treatment is not needed, red hairy roots can be visually screened to identify and induce the positive plants, and the method is simple, rapid and efficient and can be used for identifying and inducing the positive plants of the cucurbitaceae plants such as bottle gourds and the like. And the gene is transferred into an exogenous gene or is subjected to gene editing on the cucurbitaceae plant, so that the gene has important significance.

The invention discloses an application of a BnaMYB7 gene or an encoded protein thereof in regulating and controlling the oil content of rape, and belongs to the fields of genetic engineering and crop genetic breeding. The nucleotide sequence of the BnaMYB7 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, an agrobacterium-mediated genetic transformation method is used, an overexpression vector of the BnaMYB7 gene and a CRISPR / Cas9 gene editing vector are respectively transformed into a genome of the rape, the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is obtained, and the rape germplasm with overexpression and function deletion of the BnaMYB7 gene is determined by measuring the oil content of seeds of overexpression and mutant materials. It is found that the BnaMYB7 gene positively regulates the oil content of the rape, so that rape varieties with different oil contents can be obtained by using the gene to perform molecular breeding on the rape. The feed additive is of great significance in relieving grain and oil tension and guaranteeing feed safety.

The invention provides a tissue culture method for inducing cluster buds of paeonia delavayi embryo, and relates to the technical field of tissue culture of paeonia delavayi. The tissue culture method mainly comprises the steps of explant acquisition, embryo germination culture, embryo cluster bud induction culture, cluster bud multiplication culture, cluster bud rooting culture, acclimatization and transplantation and the like. According to the method, the defects in the prior art are overcome, the embryos in fresh and mature Paeonia delavayi seeds in the current year are used as explants, a regeneration system of an embryo cluster bud induction way is preliminarily established through embryo cluster bud induction, proliferation and rooting culture medium research, and a foundation is laid for establishment of a Paeonia delavayi genetic transformation system.

A novel maize variety designated X18V354 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X18V354 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X18V354 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X18V354, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X18V354 are provided. Methods for producing maize varieties derived from maize variety X18V354 and methods of using maize variety X18V354 are disclosed.

The invention relates to the field of plant genetic engineering, and discloses application of a ZmWOX5b gene in improvement of corn genetic transformation efficiency. By separating the ZmWOX5b gene and constructing a recombinant vector, an expression cassette or a recombinant bacterium containing the gene, genetic transformation is carried out on corn inbred lines B73, B104, Zheng 58, FSJ115 and Xiang 249 by utilizing an agrobacterium-mediated method, and a research result shows that the gene can remarkably improve the transformation efficiency. Compared with a traditional method, the method has the advantages that the efficiency of easily-transformed genotypes is effectively improved, meanwhile, the transformation effect of difficultly-transformed maize inbred lines is greatly improved, and the limitation of receptor genotypes in maize genetic transformation is effectively broken through. The achievement obviously widens the application range of corn genetic transformation, and provides a new tool for efficiently developing gene function research and application of high yield, high quality, disease and pest resistance, stress resistance and the like of corn.

The invention relates to the technical field of plant genetic engineering, in particular to a genetic transformation method of prunus mume line plum blossom leaf calluses. The method comprises the following steps: firstly preparing a plum blossom genetic transformation receptor, then transforming agrobacterium tumefaciens by taking an RUBY gene as a reporter gene to prepare an infection bacterium solution, then carrying out callus infection and co-culture, and finally screening to obtain the resistant callus. According to the method provided by the invention, the resistant callus can be identified in an observation or PCR (Polymerase Chain Reaction) detection mode. The plum blossom callus is successfully infected, the red callus expressed by the RUBY reporter gene is obtained, the inductivity is high, and a good basis is provided for establishment and genetic improvement of a plum blossom genetic system.