32 results about "Root nodule" patented technology

The invention discloses three growth-promoting bacteria combined with sinorhizobium freudenreanum and application thereof, and belongs to the technical field of microorganisms. According to the invention, three growth-promoting bacteria of fish pseudomonas ZMC01, pseudomonas puticola ZMC129 and cellulomicrobe vinckii ZMC94 are separated from the root of an oxytropis glabra plant in a saline-alkali beach, and the three growth-promoting bacteria have the ability to grow in a saline-alkali environment. Tests prove that the growth-promoting bacteria ZMC01, ZMC129 and ZMC94 can promote the growth of soybeans in a saline-alkali environment or a non-saline-alkali environment by independently acting or jointly acting with the sinorhizobium freudenreanum, and indexes such as plant height, root length, stem and leaf fresh weight, root fresh weight, root nodule number and root nodule fresh weight of plants are remarkably improved or increased. The growth-promoting bacteria can also improve the nodulation capacity of the sinorhizobium freudenreanum in a saline-alkali environment, the nodulation and nitrogen fixation capacity of the soybeans under saline-alkali stress is expected to be fundamentally improved, and the growth-promoting bacteria have important significance for guaranteeing safe production of the soybeans.

The application discloses application of a soybean GmGID1-2 gene in regulation of soybean plant type, nitrogen utilization efficiency and high yield and high oil. The application of the GmGID1-2 gene in soybean variety improvement, the GmGID1-2 gene CDS sequence is shown as SEQ ID NO. 1, and knocking out the GmGID1-2 gene can improve the plant type (reduce plant height, increase branch number, main stem node number and pod number), improve stem diameter and stem strength, enhance nodule nitrogen fixation, and improve yield and seed oil content. The application digs out a one-factor-multiple-effect gene GmGID1-2, generates two new mutations of the GmGID1-2 through a gene editing technology, and causes protein early termination. Knocking out the GmGID1-2 gene simultaneously realizes improvement of multiple traits, and has great application potential and market prospect in improvement of plant type and stem strength of crops and plants such as soybean, realization of high yield and high oil, nitrogen efficiency and the like.

The application discloses a high-yield cultivation method of soybean. Under the premise of increasing the seedling density, the method realizes the robust but not excessive vegetative growth of the soybean, promotes the growth of the nodule, increases the nodule nitrogen fixation amount, and ensures the sufficient supply of nitrogen, phosphorus and potassium in the soil after the grain filling stage of the soybean, so as to provide the guarantee for the high light efficiency growth of the soybean after the grain filling stage. The cultivation method has strong operability, can effectively reduce the risk of high pod drop rate in the later growth period caused by the leaf growth and podding habit of the soybean, significantly improves the group ventilation and light transmission condition of the soybean after the grain filling stage, improves the light energy utilization rate, and further improves the yield and quality of the soybean. The application has important application value.

The present disclosure relates to enhancing nitrogen fixation in legumes grown under high nitrate or nitrate stress conditions. In particular, the present disclosure relates to genetically modified plants with altered levels or expression of FUN or downstream targets of FUN, as well as methods for producing and growing the same. The present disclosure also relates to nodule senescence regulated by FUN and its downstream targets, and the regulation of FUN activity or expression by cellular zinc.

The application provides a method for inducing transgenic hairy root formation of Lagenaria vulgaris by non-tissue culture, comprising the following steps: (1) Lagenaria vulgaris culture: sowing Lagenaria vulgaris seeds in a seedling substrate, and culturing in a light incubator until the Lagenaria vulgaris plant grows to a stage between one heart and one leaf and one heart; (2) infection by a hole-punching and smearing method: taking the cotyledon node to 1 cm below the cotyledon node of the Lagenaria vulgaris plant as an infection site, punching a hole around the infection site, and smearing the hole with Agrobacterium rhizogenes; (3) nodule induction: continuing to culture the Lagenaria vulgaris plant in the light incubator, and observing that the infection site is swollen; and (4) inducing hairy root formation by cutting and culturing: obliquely cutting the swollen position of the infection site of the Lagenaria vulgaris plant, and inserting the lower end of the obtained upper plant, i.e. the obliquely cut end, into a growth carrier for culture to induce hairy root formation. The application can induce transgenic hairy root formation of Lagenaria vulgaris, has a high induction rate, and can be applied to promoter function research, functional gene analysis, secondary metabolite research and the like.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The present application belongs to the field of microbial technology. More particularly, it relates to a salt-tolerant Bradyrhizobium sp. strain RY15 and its application. The Bradyrhizobium sp. strain RY15 provided by the present application has excellent salt tolerance and can significantly promote the growth and development and nodule fixation ability of Desmodium velutinum. The pot experiment results prove that the application of the RY15 strain can significantly increase the fresh weight of the aboveground part, the fresh weight of the underground part and the number of nodules of Desmodium velutinum; in the saline soil field, the yield of the pasture is increased by 68.89% after the inoculation of the RY15 inoculant. The RY15 strain of the present application can be made into a liquid or solid microbial inoculant, which is suitable for seed dressing, root soaking or soil application, and is simple to operate, low in cost and easy to popularize. The RY15 strain of the present application is a natural symbiotic bacterium, is safe and non-toxic, can improve the nitrogen cycle of soil, expand the planting of high-quality leguminous forage in saline-alkali soil, and has good application prospect and application value.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The application discloses a Bradyrhizobium sp. and application thereof, and belongs to the technical field of nitrogen-fixing symbiotic microorganisms. The Bradyrhizobium sp. is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 27064. The strain has good growth-promoting characteristics, and has strong nitrogen tolerance and broad spectrum. In the case of reducing the nitrogen fertilizer application amount, the strain can significantly increase the number of rhizobia, the number of lateral branches, the length of main stems and the number of fruit needles of peanuts in the flowering stage, and improve the yield of peanuts in the maturing stage, thereby providing a strain source for obtaining rhizobium agents suitable for peanut planting of various varieties, providing a technical basis for reducing nitrogen fertilizer application and saving planting cost, providing an efficient and stable growth-promoting microbial germplasm resource, expanding oil crop symbiotic germplasm resources in China, and providing a basis for research and development of efficient and stable microbial fertilizers and application technologies.

The invention discloses cold region alkali-resistant rhizobium japonicum HK601 and application thereof, and relates to rhizobium japonicum and application thereof. The cold region alkali-resistant rhizobium japonicum is bradyrhizobium japonicum HK601, and the cold region alkali-resistant rhizobium japonicum is applied to soybean planting. The invention further discloses a preparation method of the cold region alkali-resistant rhizobium japonicum. The rhizobium japonicum HK601 can remarkably promote soybean nodulation, and meanwhile after the rhizobium japonicum HK601 is inoculated, the chlorophyll content, the nitrogen content, the overground part fresh weight and the plant dry weight of the soybean blackfamily 60 are improved to different degrees. The rhizobium japonicum HK601 can remarkably promote soybean nodulation, and the fresh weight of the overground part and the fresh weight of root nodules of soybeans are remarkably increased. The rhizobium japonicum HK601 disclosed by the invention can effectively grow in alkaline environments with the pH value of 10 and the pH value of 11, which shows that the rhizobium japonicum HK601 has excellent alkali-resistant characteristic.

The present application belongs to the field of agricultural technology, and particularly relates to a method for reducing the content of heavy metals in tobacco leaves and reducing the number of tobacco root nodules. The present application can significantly reduce the content of heavy metals in tobacco leaves by using biomass materials to replace all special tobacco fertilizers in combination with specific tobacco planting processes. The results of examples show that, compared with traditional special tobacco fertilizers, the biomass method treated tobacco increases yield by 8%, not only the content of heavy metals is significantly reduced, but also the biomass material can significantly reduce the number of tobacco root nodules; and further promote the growth of tobacco and improve the quality of tobacco.

The present disclosure relates to a modified plant SYMRK polypeptide that constitutively induces symbiotic organogenesis at a higher level than an unmodified plant SYMRK polypeptide under the same conditions, or induces symbiotic organogenesis in the absence of rhizobium and / or arbuscular mycorrhizal fungi recognized by a plant, and its use in a plant.

The invention discloses a medicago truncatula MtSPP1 gene and application thereof. The nucleotide sequence of the medicago truncatula MtSPP1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of protein expressed by the gene is as shown in SEQ ID NO.2. The medicago truncatula MtSPP1 gene has a nucleotide sequence as shown in SEQ ID NO.1. The medicago truncatula MtSPP1 gene is applied to increase of the soybean yield and the nodulation number. The MtSPP1 transgene provided by the invention can obviously improve the yield of stems and leaves of the overground part of the soybean, and has important application value in molecular breeding of feed type soybean. The MtSPP1 transgene can obviously improve the grain yield of soybeans, and has important application value in molecular breeding of oil and edible soybeans. The MtSPP1 transgene can significantly increase the number of root nodules of soybeans, has important application value in molecular breeding of soybeans for green manure, and is suitable for popularization and application.

The invention provides identification and application of symbiotic interaction receptor kinase of leguminous plants. The brand-new receptor-like kinase AMK2 (AM-induced kinases) which is formed by colonization of positive-tuned arbuscular mycorrhizal fungi and infection of negative-tuned rhizobium is disclosed for the first time, and the gene is induced by mycorrhiza and is necessary for symbiosis of mycorrhiza. And the AMK2 is inhibited by rhizobium, and the amk2 mutant has a phenotype with increased infection events. The invention discloses a new entry point for analyzing the specific regulation and control of the nodule symbiosis and mycorrhizal symbiosis signal transmission, and has important significance for deeply exploring the regulation and control mechanism of reciprocal symbiosis.

The invention belongs to the technical field of compound fertilizers, and particularly relates to a compound fertilizer for red kidney beans as well as a preparation method and application thereof. The compound fertilizer adopts a core fertilizer-coating structure, and the core of the compound fertilizer is that the core fertilizer comprises a composite microbial inoculum which is prepared by compounding rhizobium RH64 and lysinibacillus fusiformis according to the concentration ratio of 1: 2-2: 1, and synergistic urea, diammonium phosphate, potassium chloride, borax, sodium molybdate, fulvic acid and other nutritional and functional components. Field test results show that the compound fertilizer significantly improves nitrogenase activity of root nodules of red kidney beans, thereby effectively promoting growth of the red kidney beans, achieving great yield increase, and significantly increasing crude protein and total dietary fiber content in grains. The invention provides an efficient and reliable solution for solving the continuous cropping obstacle of the red kidney beans and improving the yield and quality of the red kidney beans.

To provide a new composition containing a microorganism whose nematicidal activity has been newly found, and to provide its use and the like.SOLUTION: One embodiment of the present invention is a nematicidal composition containing one or more kinds of fungi selected from the group consisting of Blastomonasnatatoria, Methylobacteriumradiotolerans, a fungus of the genus Agromyces, which is Agromycesallii or Agromycesarachidis, and a fungus of the genus Rhizorhabdus, which is Rhizorhabdusitchiii, Rhizorhabdusargentea, Rhizorhabdusphycosphaerae, or Rhizorhabdusdicambivorans, or containing a culture supernatant of the fungi.SELECTED DRAWING: None

The application provides a kind of based on ginkgo root secretion regulation alfalfa nodulation growth-promoting forest-grass interplanting method.The method makes full use of ginkgo under forest space, by land preparation and fertilization between forest rows, constructs high ridge square, selects high-quality alfalfa seed for strip sowing, combined with reasonable spacing and fixing seedling, cultivation and weeding and other field management measures, realize the normal growth and efficient utilization of alfalfa, provide a scientific and reasonable alfalfa interplanting mode.The application combines soil physical and chemical index determination, systematically evaluates forest soil before and after alfalfa planting, confirms that the method can effectively improve soil physical and chemical properties, promote ginkgo-alfalfa synergistic growth, has significant ecological benefits and application prospect.In addition, the application further verifies that ginkgo root secretion has obvious growth-promoting effect on alfalfa rhizobium, and can improve the expression level of alfalfa nod gene, enhance the number and quality of nodule, so as to improve the production performance of alfalfa and soil improvement effect.

The application belongs to the technical field of seed coating agents, and particularly relates to a formula and application of a soybean yield-increasing seed coating agent, wherein the coating agent components are as follows in terms of weight parts: distilled water 45-55 parts, ammonium sulfate 10-20 parts, potassium dihydrogen phosphate 10-20 parts, insecticide 10-20 parts, preservative 0.2-0.7 parts, pigment 1 part, film forming agent 3-8 parts, and melatonin 25-200 micromoles per liter. The soybean leaf chlorophyll content, nodule number and yield of the soybean planted with the seed coated with the melatonin-containing coating agent are all significantly improved compared with the soybean planted with the seed coated with the coating agent without melatonin.

The application provides application of transcription factor STY2 in regulation of nodule development. The application provides a method for improving nitrogen fixation capacity of a nodule plant or a method for preparing a nodule plant with increased nitrogen fixation capacity, comprising: up-regulating expression or activity of STY2 in the nodule plant.

The invention discloses rhizobium leguminosarum and application thereof in growth promotion of peas, and belongs to the field of microorganisms. The rhizobium leguminosum is preserved in China Center for Type Culture Collection on April 2, 2025, the address of the rhizobium leguminosum is No. 299, the address of the rhizobium leguminosum is eight road, Wuchang District, Wuhan City, Hubei Province, the postal code of the rhizobium leguminosum is 430072, and the preservation number of the rhizobium leguminosum is CCTCC NO: M 2025681. The invention also provides an application of a culture, a fermentation broth, a fermentation supernatant, a bacterial suspension, a liquid bacterial agent, bacterial powder or a compound microbial agent of the rhizobium leguminosarum in increasing the number of pea nodules and promoting the growth of pea plants. The rhizobium leguminosarum provided by the invention has a good nodulation effect, and most of nodules are effective nodules. Especially, the number of pea nodules can be obviously increased.

The application discloses a special bacterial fertilizer for red bean grass and belongs to the technical field of microorganisms. The special bacterial fertilizer for red bean grass comprises the following components: a bacterial strain Rhizobium yanglingense GAU-00008, a bacterial strain Bacillus sp. CHO87, Bacillus subtilis LSH11 and a carrier. Through the synergistic effect of the three bacterial strains, the special bacterial fertilizer for red bean grass can make the red bean grass roots form sufficient and effective nodules, activate the soil, promote the absorption of other nutrient elements by crops, improve the nutrient structure of plants, regulate and promote the growth of plants and enhance the disease resistance of plants.

The invention provides a special biological full-effect topdressing-free compound fertilizer for soybeans and a preparation process thereof, and belongs to the technical field of agricultural fertilizers. Comprising an inner core component, an outer coating fungicide layer and an anti-caking oil layer. According to the invention, mineral source humic acid and coniferous forest natural degraded lignin are added into a core formula to form a double-organic-matter slow-release system, and a nitrification inhibitor dicyandiamide (DCD) and a urease inhibitor (NBPT) are matched, so that staged controlled release of nitrogen is realized, and compared with a traditional formula, the fertilizer efficiency period is prolonged; the outer microbial inoculum coating layer adopts potassium fulvate to construct a composite carrier, a bacillus mucilaginosus-rhizobium japonicum compound system is loaded, through carbon source supply of the potassium fulvate, the field colonization rate of the microbial inoculum is increased, and phosphorus and potassium activation and biological nitrogen fixation are effectively promoted. Field tests show that the number of soybean root nodules can be increased, the topdressing frequency can be reduced, the grain protein content can be increased, and the nitrogen fertilizer input cost can be reduced while the yield is increased.

The invention relates to a method for preventing or reducing plant gall formation, the method comprising applying on a plant a composition comprising a suitable amount of at least one ingredient selected from the group consisting of cytokinins, auxins, or auxin precursors. The auxin is preferably indole-3-butyric acid (IBA). The invention further relates to a method for treating seeds. The invention further relates to the use of a composition comprising a suitable amount of an auxin or an auxin precursor, for the prevention and / or reduction of plant gall formation. The gall formation is preferably caused by clubroot disease.

Aspects of this disclosure relate to genetically modified plants comprising NODULE INCEPTION (NIN) and NIN-LIKE PROTEIN (NLP) that have been genetically altered to respond to auxin, so that the NIN or NLP protein can induce root nodulation following appropriate signaling.

The invention provides a regulatory gene for nitrate tolerance in symbiotic nitrogen fixation of leguminous plants and application of the regulatory gene. In the invention, a novel functional gene SUMO (including SUMO1 or SUMO2) is researched and revealed for the first time and encodes SUMO protein with important biological functions, and the protein participates in the symbiotic nitrogen fixation process of leguminous plants and rhizobium and participates in the nodulation inhibition process of nitrate, can regulate and control important components in nitrate signal transduction and symbiotic nitrogen fixation, and can be used for preparing the novel functional gene SUMO (including SUMO1 or SUMO2). The molecular mechanism of nitrogen in inhibiting the symbiotic process of leguminous plant root nodules is analyzed. The invention discloses a new mechanism for regulating and controlling the characters of leguminous plants, and has important significance in the aspects of genetic improvement of plant characters and soil improvement.

A non-destructive real-time plant root imaging and analysis system to detect gall-forming plant-parasitic nematodes is disclosed. The system includes a root imaging device based on a minirhizotron concept with customizations and additions such that it can be inserted into the soil in the vicinity of a plant root and subsequently take images of the plant root system at multiple positions and angles. The device includes a movable base equipped with a high-resolution digital camera module integrated into the transparent tube of a minirhizotron, allowing images of the host plant root at different depths to be taken when the base travels within the transparent tube. A set of image processing algorithms are developed and integrated into a custom-designed graphic user interface (GUI) to analyze and detect these nematodes' presence automatically. The algorithms may stitch the images taken to obtain a panoramic view of the entire plant root system.

The application provides a multifunctional Aenibacillus terrae capable of degrading organophosphorus pesticides and resisting diseases, the strain is Aenibacillus terrae, and is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No. 30973. The strain can degrade organophosphorus pesticides, including profenofos, malathion, diazinon, phoxim and fenthion, and the degradation effect on diazinon is the best; the strain can inhibit the growth and development of southern root-knot nematodes, and prevent tomato root knot disease caused by the nematodes; the strain can antagonize fusarium oxysporum, and prevent tomato fusarium wilt caused by the fusarium oxysporum; and the strain can increase the supply of soil available phosphorus, and promote the growth of leafy vegetables.

This invention provides the application of the MYB3R1 gene in regulating the number of nodules in rhizophyllum plants. This invention is the first to study and reveal a novel gene, MYB3R1, that can regulate the number of nodules in rhizophyllum plants; relatively high MYB3R1 expression indicates an increase in the number of nodules. MYB3R1 is a promising gene for plant genetic improvement. This invention also discloses a novel mechanism for regulating the nodule trait in rhizophyllum plants, which has significant theoretical implications for the genetic improvement of plant traits.

The application belongs to the technical field of plant genetic engineering and soybean breeding, and discloses a gene GmCDPKb Application of the gene in regulating the number of symbiotic nodule of soybean, GmCDPKb The nucleotide sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The application constructs GmCDPKb The mutant and overexpression soybean stable genetic transformation plants are obtained, through trait investigation, it is found that the nodule number of the mutant plant is significantly lower than that of the wild type, and the nodule number of the overexpression plant is significantly higher than that of the wild type. It is shown that GmCDPKb The gene plays a regulatory role on the nodule number of soybean. The application discloses that GmCDPKb The gene is a key regulatory factor for regulating the number of symbiotic nodule of soybean, the nodule number of soybean can be stably and significantly regulated by regulating the expression amount of the gene, and important gene resources and germplasm materials are provided for analyzing the symbiotic nitrogen fixation molecular mechanism of soybean and cultivating high-efficiency nitrogen fixation soybean new varieties.

The application belongs to the field of agricultural microorganism technology, and discloses rhizobium for improving the feeding performance of soybean, a bacterial agent and application thereof. The rhizobium is Sinorhizobium fredii QYS03, the preservation number of which is CCTCC NO: M 20252632, and is isolated from the rhizobium of a feeding type soybean in a northwest loess upland area. The rhizobium has the abilities of dissolving inorganic phosphorus, organic phosphorus and secreting indole acetic acid, and has a broad spectrum of nodulation characteristics. The application further provides a rhizobium bacterial agent containing the strain and a preparation method thereof, and the number of viable bacteria in the bacterial agent is not less than 2*10 9 cfu / mL. The rhizobium and the bacterial agent can be applied to soybean planting by seed dressing, can significantly promote the growth of soybean stems and leaves and root systems, improve the nodulation and nitrogen fixation capacity, increase the biomass accumulation of soybean, improve the soil fertility, and have a broad application prospect in the field of soybean forage cultivation.