196 results about "Root nodule" patented technology

The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

The invention belongs to the technical field of agriculture and environmental pollution improvement and relates to a heavy metal resistant plant endogenous nodule bacterium and application thereof. The heavy metal resistant nodule bacterium is preserved at the China center for type culture collection (CCTCC) with the preservation date to be 2012/9/18 and culture preservation number to be CCTCC NO:M2012357. The bacterial strain liquid preparation contains more than a billion of effective living bacteria per milliliter, and the bacterial strain solid preparation contains 0.2 billion of effective living bacteria per gram. The heavy metal resistant nodule bacterium strain can secrete plant hormones indole acetic acid (IAA) and siderophores, and resists heavy metal copper and cadmium. When plants are planted in moist soil or tailings containing heavy metal and bioremediation preparation is inoculated, the heavy metal resistant nodule bacterium can promote plant growth and absorbs heavy metal copper, can improve plant restoration efficiency, can fix sandy soil and reduces water and soil loss.

The invention relates to an interplant method of camellia oleifera and torreya grandis young growth. The interplant method of the camellia oleifera and the torreya grandis young growth is characterized in that peanut and upland rice are interplanted at intervals between the camellia oleifera and the torreya grandis young growth, and the peanut and the upland rice interchange positions in the nest year. The interplant method of the camellia oleifera and the torreya grandis young growth has the advantages that the upland rice provides shade for the camellia oleifera or the torreya grandis so that expense of sheltering nets is reduced, interplanted the upland rice and the peanut can store water and keep moisture for the torreya grandis young growth so as to avoid water and soil loss, the peanut provides nitrogen needed during in the early stage of production of the camellia oleifera by means of a biological nitrogen fixation function of the nodule bacteria so that nitrogenous fertilizer is saved, intercropping and crop rotation of the interplanted crop can not only avoid damage, caused by continuous cropping, to the soil but also have functions of restraining occurrence and spread of plant diseases and insect pests and isolation, tend is replaced with cultivation, weeds are restrained, management cost of the camellia oleifera or the torreya grandis is reduced, the utilization rate and the copping index of the forest land are improved and economic benefits are increased.

The invention relates to a compound microbial preparation for promoting paddy growth and a preparation method thereof. The compound microbial preparation for promoting paddy growth is characterized by consisting of 20-40 parts by weight of a microbial strain culture and 60-80 parts by weight of a microbial carrier; wherein, the microbial strain culture consists of the following raw materials in parts by weight: 2-8 parts of bacilli subtilis, 2-8 parts of bacilli megaterium, 5-10 parts of saccharomyces cerevisiae and 1-4 parts of rhizobia; the microbial carrier is zeolite or bentonite or mixture thereof. The compound microbial preparation has the advantages of promoting growth of beneficial microbes, enhancing absorption of nitrogen, phosphorous, potassium and other organic substances for paddy root system and promoting normal growth of the root system, thus promoting the paddy growth and reducing chemical fertilizer application.

The invention discloses a phosphorus transportprotein gene GmPT5 related to phosphorus transport of soybean nodulation and application thereof. The phosphorus transportprotein gene GmPT5 related to the phosphorus transport of the soybean nodulation has a nucleotide sequence as shown in SEQ ID NO:1, and coded protein nucleotide sequence is shown as SEQ ID NO:2. The gene GmPT5 regulates and controls the phosphorus transport from a root part to the nodulation, furthermore, the overexpression of the gene is favorable for increasing the size of the soybean nodulation and the plant biomass as well as the nitrogen and phosphorus contents.

The invention discloses a method for remediating polycyclic aromatic hydrocarbon (PAH) polluted soil through cooperation of alfalfa and complex microbial inoculants. Alfalfa seeds are pretreated, and the plant sprouting period is shortened by 2-3 days, so that the tolerance of the plant to soil containing PAHs is enhanced, and the remediating capacity of the plant on the soil is improved. Besides, in an alfalfa-trichoderma-nodule bacterium combined remediating system, the synergistic effect of trichoderma and nodule bacteria is used for promoting degradation of the alfalfa to the PAHs in the polluted soil. Meanwhile, the physiological contour of the microbial population level of alfalfa rhizosphere soil is changed, and the diversity and the stability of the microbial ecological functions of the soil are recovered, so that the growth environment of crops is improved, and the quality and the yield of the crops are improved; the method has wide market application prospects.

The invention relates to a method for improving a plant planting desert, which is realized by the following steps: adding 140 to 170kg of liquid film, 0.6 to 8kg of calcium magnesium phosphate, 0.01 to 0.2kg of ammonium molybdate and 0.4 to 5kg of nitragin of this family into 800 to 1300kg of water, stirring, dissolving, then adding 4 to 50kg of leguminous forage seed, stirring evenly, sowing on the sand of one hectare and enclosing and banning grazing for 3 to 5 years; and sowing 7 to 30kg of gramineous forage grass seed on the sand after the primary period, relieving enclosure and grazing banning after enclosing and banning grazing for 2 to 3 years and keeping the pasturage strength at 5 to 10 animals/year to achieve the ecological equilibrium of animals and plants so as to finish the improvement of the desert. By using the method, the usability of soil can be increased, the soil obtains sufficient water and nutrients, a woodland with large area can become a source of forest, the condition that the growth rate of the forest is greater than the exploitation rate is kept, the forest provides more oxygen gas used by organisms and assists to lower a warming phenomenon, buildings can be constructed again in part of regions with desertification reversion by the windproof protection of the forest, and the volume and the utilization rate of the soil are increased.

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

The invention provides rhizobium Scot1305 and a symbiotic system of rhizobium Scot1305 and plants is established. Due to nitrogen fixation function, siderophore secretion function and soluble phosphorus activity of the rhizobium strain and protection effect of the rhizobium strain on key enzyme of plant tocopherol synthesis, enhancement effect of the strain on phytoremediation of Co contaminated soil is realized. The strain can coexist with plants in Co contaminated soil, and can promote the extraction and enrichment of heavy metals Co in the aerial parts of plants, and finally achieve the purpose that the content of Co in soil meets the environmental safety standard.

The invention discloses a method for repairing arsenium-polluted farmland soil through microorganisms and plants in a combined manner. The method includes the following steps that firstly, a microbial agent is sprayed, wherein nodule bacteria are sprayed in the arsenium-polluted farmland soil after being dissolved in water; secondly, plant plantation is conducted, wherein Nephrolepiscordifolia(L.)Presl is planted in the arsenium-polluted farmland soil where the nodule bacteria are sprayed; and thirdly, field management is conducted, wherein the field management is conducted on the Nephrolepiscordifolia(L.)Presl, and normal growth of the plants is ensured. Treatment of arsenium-polluted farmland soil repair is conducted through the microorganisms and the plant repair technology in a combined manner, the nodule bacterium NT-26 and Nephrolepiscordifolia(L.)Presl combined repair is adopted, the leaching concentration of the heavy metal arsenium in the repaired and treated farmland soil is smaller than the IV-type standard in Quality Standard for Ground Water (GB/T14848-93), the whole construction process is high in benefit, and influences on the environment are small.

The invention discloses a rhizobium strain and its application. The rhizobium strain is named as Rhizobium sp., the strain number is zb612, and preservation number is CGMCC No. 10721. The rhizobium strain was preserved in the China General Microbiological Culture Collection Center (CGMCC) at the No.3, 1 West Beichen Road, Chaoyang District of Beijing on April 15th, 2015. The strain provided by the invention can be used in onsite real nitrogen removal from sewage so as to raise the effect of removing ammonia nitrogen from oil sludge. The rhizobium provided by the invention has the characteristic of removing ammonia nitrogen. According to the rhizobium strain, organic carbon can be used as the sole carbon source and ammonia nitrogen is used as the sole nitrogen source so as to carry out metabolism, and ammonia nitrogen is directly converted to N2 through heterotrophic nitrification-aerobic denitrification so as to finish nitrogen removal. Thus, problems of aerobiotic nitrification and anoxic denitrification sectional treatment in traditional biological removal of nitrogen are solved.

The invention discloses a microecological modulator. The microecological modulator is prepared from photosynthetic bacteria, actinomycetes, bacillus, saccharomycetes, lactic acid bacteria, nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium bacteria, silicate bacteria, bacillus radicicola, blue-green algae, vitamin C, nitrogen, boron, iron, saccharose, calcium sulfate, dipotassium phosphate, calcium carbonate, magnesium sulfate, sodium chloride and agar. According to the microecological modulator disclosed by the invention, by adopting a fusion technique of multiple useful microbiological populations, the useful microbiological population and plant enzymes are fermented and cultivated at the same time, mutual phagocytosis of biological populations in a fusion process of the biological populations can be prevented, and symbiosis and compatibility are realized. The microecological modulator disclosed by the invention is rich in multiple microbiological populations and multiple trace elements and mineral substances needed by plant growth, so that microecological circulation of soil can be improved, and the fertilizer efficiency of the soil is enhanced; the microecological modulator replaces chemical fertilizer; photosynthesis of leaf surfaces of plants and crops is enhanced, the oxidation is reduced, and leaf surfaces of the plants and the crops can be lustrous and plump; the plants and the crops can be full in fruits; the yields of the plants and the crops can be increased.

The invention relates to the technical field of agricultural planting, and particularly relates to a method for rotation of alfalfa and awnless brome. The method for rotation of alfalfa and awnless brome comprises the following steps: turning over and planting alfalfa for 5-6 years after planting the awnless brome for 5-6 years, and then turning over and planting the awnless brome, and carrying out crop rotation repeatedly. The method has the beneficial effects that the yield and the soil fertility of high-quality forage per unit area are improved in a crop rotation manner, the physical and chemical properties of soil are improved, nitrogen in atmosphere also can be fixed by root nodules of alfalfa, and the soil fertility is improved. Thus, the fertilizing amount can be correspondingly reduced, investment in manpower and chemical fertilizer is reduced, the investment in fertilizer in the next round of awnless brome planting process also can be reduced, the yield of the awnless brome is increased, the land utilization rate and the acre yield are further improved, and the economic benefits are increased.

The invention relates to a nodule bacteria transferring method by utilizing distant grafting, comprising: 1) hybrid sunflowers and corns which have high plasticity are chosen as stock; 2) varieties with high nitrogen fixation activity and stable hereditary are chosen from leguminous plants; 3) cleft grafting is adopted, when 1-6 pieces of leaves of the stock grow and 1-6 pieces of leaves of the scion grow, grafting is carried out; 4) after the grafting survival is realized, the growth amount of the stock and the scion is rationally controlled; the plants with nodule bacteria and the plants without nodule bacteria are grafted by utilizing the distant grafting, so as to lead the nodule bacteria to be transferred to the plants without nodule bacteria and cause the plant without nodule bacteria to have nodule bacteria naturally.

The invention relates to a nutrient film protecting agent of rhizobium japonicum and a production method thereof. The nutrient film protecting agent of rhizobium japonicum is prepared by selecting the raw materials of PVP (Polyvinylpyrrolidone) K30, PVP S630, sodium carboxymethyl cellulose, peptone, bovine serum albumin and sodium molybdate and reacting under a certain reaction condition; the nutrient film protecting agent of rhizobium japonicum, a liquid rhizobium japonicum agent and soybean seeds are mixed and stirred according to a certain proportion; and after the soybean seeds are coated and stored for two days, each soybean seed can ensure 100,000 live rhizobium japonicums. The invention has wide needed raw material sources, simple production process and low cost, can play roles of nutrition, film formation and protection in the rhizobium japonicum, and can ensure that the liquid rhizobium japonicum agent is preserved for one year at normal temperature; the live bacterium quantity is not smaller than 2,000,000,000/ml, and the nitrogen fixing activity is not reduced.

The present invention discloses an alfalfa seed rhizobia seed coating agent and a preparation method thereof. The alfalfa seed rhizobia seed coating agent of the present invention is prepared from raw materials containing a rhizobia agent, an adhesive and a substrate, wherein the rhizobia agent is formed by uniformly mixing a rhizobia liquid and a rhizobia adsorbent, the active ingredient of the rhizobia liquid is the Sinorhizobium meliloti, the mass content of ammonium molybdate in the rhizobia agent is 0.1-0.2%, and the ammonium molybdate content in 100 g of the rhizobia agent is (0.1-0.2) g and the Sinorhizobium meliloti content in 100 g of the rhizobia agent is more than or equal to 5*10<11> cfu according to the ratio of the ammonium molybdate in the rhizobia agent to the Sinorhizobium meliloti. According to the invention, the alfalfa rhizobia and the molybdenum with the optimal concentration are matched, and the skimmed milk is added to the adhesive as the protection agent, such that the prepared alfalfa seed pill coating agent has characteristics of rhizobia activity prolonging, plant nodulation and nitrogen-fixation promoting, substantial alfalfa yield increasing and alfalfa quality improving.

The invention provides a method for preparing an anti-acetochlor soybean rhizobium inoculum, and belongs to a microbial inoculum preparation technology; the method uses soybean rhizobia with highly efficient nitrogen fixation activity to increase the concentration of acetochlor gradually in a sucrose yeast extract culture medium, and to culture resistant soybean rhizobia under the condition of acetochlor high-pressure selection to form a spawn of the anti-acetochlor soybean rhizobia after pure lien separation, and then selects the spawn into a sloped tube to form a primary strain, propagates the primary strain to form a secondary strain and inoculates the secondary strain to a fermentation tank to produce the microbial inoculum; the method has a simple process, low production cost, and the preparations prepared by the method has high performance and quality and can avoid damages to nitrogen fixation of soybean rhizobia by acetochlor during use and improve the yield and quality of soybean.