Rhizobium strain and application thereof

A technology of rhizobia and bacterial strains, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of immature application of heterotrophic nitrifying bacteria engineering

Active Publication Date: 2015-08-12
水艺环保集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the engineering application of heterotrophic nitrifying bacteria is immature

Method used

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  • Rhizobium strain and application thereof
  • Rhizobium strain and application thereof
  • Rhizobium strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Isolation and screening of rhizobia zb612

[0034] (1) Sludge domestication: Take 5g of activated sludge from a landfill leachate treatment plant and place it in 250ml ammonia nitrogen degradation medium at a temperature of 30°C and a rotating speed of 100 for two days in a continuous shaker. After two days, transfer it to an Erlenmeyer flask Add 5ml of sterilized ammonium sulfate solution (1.5g / L), continue to acclimate it, repeat this step 3 times, take out 5ml of muddy water and place it in a sterilized centrifuge tube.

[0035] (2) Limit dilution separation: Take 1ml of the above supernatant and place it in a centrifuge tube with 10ml of sterile physiological saline, and dilute it to 10 -6， , Do three parallels, from 10 -5 With 10 -6 Take a 0.2ml plate from the section and spread it on the plate, incubate for 48 hours, and observe the colony growth.

[0036] (3) Strain selection and purification: select well-growing strains from the plate for streaking, and when they gr...

Embodiment 2

[0043] Growth of Rhizobium sp.zb612 and determination of ammonia nitrogen degradation

[0044] Rhizobium sp.zb612 was inoculated into 100ml of LB liquid medium (yeast extract 5g / L, peptone 10g / L, NaCl 10g / L), and cultured at 30°C, 100rpm, shaking on a shaker. After culturing for 48 hours, the cells are divided into sterilized preservation tubes, and 0.5 ml bacterial liquid and 0.5 ml 30% glycerol are added to each preservation tube. Store at minus 80°C for later use.

[0045] One loop of the strain was inoculated into 100ml heterotrophic nitrifying bacteria culture medium, 30°C, 100rpm, shaking culture for 24h, used as the active inoculum for the formal degradation experiment, and 5% of the active inoculum was used for 2 groups of experiments, each group separately Connect three cultures containing 100ml of sterilized ammonia nitrogen degradation medium (the concentration of ammonia nitrogen is 150ml / L), shake culture at 30℃, 100rpm, one of them, take samples every few hours to de...

Embodiment 3

[0048] Aerobic denitrification test of Rhizobium sp.zb612

[0049] Add 200ml glucose nitrate medium to a 500ml Erlenmeyer flask, add the cell suspension at 2% volume, and incubate on a shaker for 48 hours to observe the residual nitrate.

[0050] The test results showed that after 48h, the residual nitrate concentration was 48.4mg / L, nitrite was very little accumulated, the nitrate concentration in raw water was 352.7mg / L, and the degradation rate was 86.2%.

[0051] The test results show that under aerobic conditions, Rhizobium zb612 has the function of aerobic denitrification, and there is almost no accumulation of nitrite.

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Abstract

The invention discloses a rhizobium strain and its application. The rhizobium strain is named as Rhizobium sp., the strain number is zb612, and preservation number is CGMCC No. 10721. The rhizobium strain was preserved in the China General Microbiological Culture Collection Center (CGMCC) at the No.3, 1 West Beichen Road, Chaoyang District of Beijing on April 15th, 2015. The strain provided by the invention can be used in onsite real nitrogen removal from sewage so as to raise the effect of removing ammonia nitrogen from oil sludge. The rhizobium provided by the invention has the characteristic of removing ammonia nitrogen. According to the rhizobium strain, organic carbon can be used as the sole carbon source and ammonia nitrogen is used as the sole nitrogen source so as to carry out metabolism, and ammonia nitrogen is directly converted to N2 through heterotrophic nitrification-aerobic denitrification so as to finish nitrogen removal. Thus, problems of aerobiotic nitrification and anoxic denitrification sectional treatment in traditional biological removal of nitrogen are solved.

Description

Technical field [0001] The invention belongs to the technical field of environmental protection, and specifically relates to a rhizobia strain with heterotrophic nitrification, aerobic and denitrification functions and its application. Background technique [0002] Ammonia nitrogen is one of the main pollutants of water pollution. Excessive ammonia nitrogen can cause deterioration of water quality, eutrophication of the water body, and the main cause of a large number of deaths of aquatic animals. Ammonia nitrogen degradation can be divided into biological denitrification and physicochemical denitrification, but as the most economical and effective method, biological denitrification is also the most promising. However, the traditional biochemical denitrification method requires aerobic nitrification and anoxic denitrification. The process and technology are complicated and the cost is relatively high. Therefore, the separation and application of heterotrophic nitrifying bacteria ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/41
CPCC02F3/02C02F3/34C12N1/205C12R2001/41Y02W10/10
Inventor 邹艳艳梅荣武韦彦斐李明智张宇王慧荣许青兰
Owner 水艺环保集团股份有限公司
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