49 results about "Rhizobium" patented technology

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention belongs to the technical field of fertilizers and relates to a compound fertilizer for black beans as well as a preparation method and application thereof. The compound fertilizer comprises a basic fertilizer, a microbial fertilizer and a trace element fertilizer, and a preparation method of the microbial fertilizer comprises the following steps: treating lignite with sophorolipid and trehalose lipid, treating turf with surfactin, mixing the treated lignite and turf, and then inoculating bacillus subtilis and rhizobium to obtain the microbial fertilizer. Through a unique dual mechanism of carrier synergism and fungicide function complementation, the dry matter cumulant and nitrogen cumulant of the overground part of the black bean are remarkably increased, and finally the yield and quality are remarkably improved.

This invention discloses a genetically engineered bacterium and method for enhancing the denitrification efficiency of anaerobic ammonia oxidation at low temperatures. The genetically engineered bacterium is *Escherichia coli* expressing the cold shock protein gene *cspB*. The preparation method of this genetically engineered bacterium is as follows: (1) the cold shock protein gene *cspB* is ligated into the *pET-28a(+)* plasmid to obtain the *pET-28a(+)*-*cspB* plasmid; (2) the *pET-28a(+)*-*cspB* plasmid is introduced into *E. coli*, and after screening to obtain positive clones, it is cultured to obtain the genetically engineered bacterium. The method for enhancing the denitrification efficiency of anaerobic ammonia oxidation at low temperatures is as follows: the bacterial solution of the above-mentioned genetically engineered bacterium is mixed with the bacterial solution of *Rhizobium* to obtain a mixed bacterial solution; the mixed bacterial solution is placed at 2-8℃ for 4-8 hours for cold shock; the cold-shocked mixed bacterial solution is added to the anaerobic ammonia oxidation reactor. This invention can effectively improve the denitrification efficiency and operational stability of the anaerobic ammonia oxidation process at low temperatures.

This novel nonpathogenic bacterial strain exhibiting a controlling effect on crown gall disease in plants and tomato canker disease is the Allorhizobium vitis A3 strain (accession number: NITE BP-041154).

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The application discloses a 5-aminoacetylpropionic acid-producing genetically recombined bacillus velezensis and application thereof, relates to the technical field of biotechnology and microbial metabolic engineering. The bacillus velezensis is obtained by integrating a hemA gene of Bradyrhizobium japonicum into the genome of bacillus velezensis R9, and the bacillus velezensis is obtained. The bacillus velezensis R9 has been preserved in the China Center for Type Culture Collection on July 5, 2023, and the preservation number is CCTCC NO: M 20231199. The strain can ferment to produce 17 mg / L of 5-aminoacetylpropionic acid in a 5-aminoacetylpropionic acid detection culture medium, and the concentration is more than 3 times higher than that of the bacillus velezensis R9, so the strain is an ideal engineering strain for producing 5-aminoacetylpropionic acid.

This invention relates to a compound microbial agent for the prevention and control of tobacco black shank disease, belonging to the field of biotechnology. The compound microbial agent comprises a suspension of *Pseudomonas aeruginosa* 232, *Bacillus cereus* 183, and *Mesophytes stolonifer* 161 in a volume ratio of 3:1:3, wherein the effective viable count in each of the aforementioned three bacterial suspensions is not less than 1 × 10⁻⁶. 9 CFU / mL. The compound biocontrol agent provided by this invention can effectively control tobacco black shank disease. Experimental verification shows that after 8 weeks of application of this agent to tobacco seedlings, the biocontrol effect against tobacco black shank disease can reach 33.34%.

The application discloses a Bradyrhizobium sp. and application thereof, and belongs to the technical field of nitrogen-fixing symbiotic microorganisms. The Bradyrhizobium sp. is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 27064. The strain has good growth-promoting characteristics, and has strong nitrogen tolerance and broad spectrum. In the case of reducing the nitrogen fertilizer application amount, the strain can significantly increase the number of rhizobia, the number of lateral branches, the length of main stems and the number of fruit needles of peanuts in the flowering stage, and improve the yield of peanuts in the maturing stage, thereby providing a strain source for obtaining rhizobium agents suitable for peanut planting of various varieties, providing a technical basis for reducing nitrogen fertilizer application and saving planting cost, providing an efficient and stable growth-promoting microbial germplasm resource, expanding oil crop symbiotic germplasm resources in China, and providing a basis for research and development of efficient and stable microbial fertilizers and application technologies.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The invention discloses a composite flora for relieving continuous cropping obstacles of soybeans and application of the composite flora, and belongs to the technical field of agricultural microorganisms. The composite flora comprises novosphingobacterium kaempferiae C6, sphingobacterium canadensis C14, variovorax sp. C16 and rhizobium oak C19; the preservation number of the sphingobium kaempferiae C6 is CGMCC (China General Microbiological Culture Collection Center) NO.35781, the preservation number of the sphingobium C14 is CGMCC NO.35782, the preservation number of the variovorax sp. C16 is CGMCC NO.35783, and the preservation number of the rhizobium oak C19 is CGMCC NO.35784. The invention further discloses a preparation method of the bacterial strain. The complex microbial inoculant prepared from the complex flora is used for relieving the soybean continuous cropping obstacle, and the result shows that the screened complex flora has a remarkable relieving effect on the soybean continuous cropping obstacle, can effectively improve the growth performance of soybeans and improve the antioxidant function of the soybeans, and has wide application value.

The application discloses a kind of compound microbial flora for relieving soybean continuous cropping obstacles and application thereof, belong to agricultural microbial technology field.The compound microbial flora includes novosphingobium kandensis C6, novosphingobium canadense C14, soil voracious bacteria C16 and rhizobium quercus C19;Novosphingobium kandensis C6 preservation number is CGMCC NO.35781, novosphingobium canadense C14 preservation number is CGMCC NO.35782, soil voracious bacteria C16 preservation number is CGMCC NO.35783, and rhizobium quercus C19 preservation number is CGMCC NO.35784.The compound microbial flora is used for preparing compound microbial agent for relieving soybean continuous cropping obstacles, and the results show that the compound microbial flora screened in the application has significant relieving effect on soybean continuous cropping obstacles, can effectively improve the growth performance of soybean, improve its antioxidant function, and has wide application value.

The invention discloses a microbial complex microbial inoculant for promoting the growth of leaf vegetables, which comprises bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum, and the viable count ratio of the bacillus subtilis, the rhodopseudomonas palustris and the bradyrhizobium japonicum is 3: 1: 1. The microbial complex microbial inoculant for promoting the growth of the leaf vegetables comprises bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum, and the viable count ratio of bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum is 3: 1: 1. The preparation method of the microbial inoculum comprises the following steps: S1, respectively culturing the three bacteria to a logarithmic phase to obtain 109CFU / ml bacterial liquid; s2, mixing the fermentation broth according to a ratio, and ensuring that the viable count is accurately proportioned; s3, adding a carrier and auxiliary materials, and controlling the water content to be 35%; s4, drying at low temperature, protecting thallus activity, crushing, sieving and packaging to obtain a finished product. The three functional bacteria are scientifically proportioned, so that the synergistic growth promoting effect on the lettuce and the leaf mustard is realized, and the yield and the quality are remarkably improved.

The invention relates to an application method of a special microbial agent for improving the nitrogen fixation efficiency of soybean root nodules, and belongs to the field of agricultural microorganism application. A core strategy of staged time sequence application is adopted: firstly, before sowing or during sowing, a first complex microbial inoculant containing proline secreting bacteria and indigenous infectious microbe inhibiting bacteria is applied into soil, and a pre-culture period is performed for 5-15 days; during the period, proline is converted into indole-3-acetic acid (IAA) by a fourth microorganism which synchronously exists or is introduced, so that a growth-promoting microenvironment is established at the rhizosphere, and meanwhile, a third microorganism inhibits harmful infectious microbes; next, bradyrhizobium or sinorhizobium is applied to the seedlings or rhizosphere. According to the time sequence design, an optimized colonization ecological niche with low competition and high chemotactic signals is created for target rhizobium through a synergistic mechanism of firstly improving the environment and then introducing strains. According to the method, the infection success rate of rhizobium can be remarkably increased, the number of root nodules and nitrogenase activity are increased, soybean root development is synchronously promoted, and the soybean symbiotic nitrogen fixation efficiency and yield are improved.

The invention relates to the technical field of grassland restoration, in particular to a degraded grassland restoration microbial agent, a granular restoration microbial agent, a modifier and application of the degraded grassland restoration microbial agent and the modifier. The degraded grassland repairing microbial inoculum provided by the invention is prepared from pseudomonas fluorescens T6 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36876 and sinorhizobium meliloti NFB5 with the preservation number of CGMCC No.33618. The compounding of the pseudomonas fluorescens T6 and the sinorhizobium meliloti NFB5 in the degraded grassland repairing microbial agent provided by the invention has a synergistic effect, and has a good repairing effect on the degraded grassland and a high strain colonization rate. The method depends on local resources (sheep manure, peat soil, native microorganisms and native grass seeds) of the Inner Mongolia grassland, gives consideration to ecological restoration and animal husbandry production, realizes soil-microorganism-vegetation three-dimensional synergistic integrated restoration, and has important technical innovation value and practical application significance.

The application discloses a biological information-based genetic data mining system for Astragalus sinicus breeding, and particularly relates to the technical field of data mining; the system is characterized in that: raw sequencing data of a host genome of Astragalus sinicus and a symbiotic microorganism genome of rhizobium is collected and synchronized to generate a standardized genetic information dataset; sequence cross-contamination sites between the host genome and the symbiotic microorganism genome are identified, and genetic information interference from the microorganism is removed to obtain purified host genome data free of microorganism interference; the contribution of the microorganism genome to target traits of Astragalus sinicus is analyzed to obtain a microorganism effect weight; the purified host genome data and the microorganism effect weight are integrated to construct a trait association analysis model of Astragalus sinicus and locate trait association sites; finally, candidate breeding markers are screened based on the trait association site information to obtain a precise breeding genetic marker set; the application improves the accuracy and efficiency of Astragalus sinicus breeding.

The invention relates to a composition with an eye enlarging effect and application of the composition. The composition is prepared from soluble collagen, tocopheryl nicotinate, acetyl hexapeptide-8, hydrolyzed rice protein, acetyl tetrapeptide-5, Arabic gum, rhizobium gum, an arnica extract and schizophyllum commune fermentation product filtrate. By compounding a plurality of plant extracts and polypeptides for use, excellent effects of tightening, lifting, removing pouches and reducing melanin can be realized, and the eye cream has a visual eye enlarging effect and is more young.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

The invention discloses a magnesium modified wheat straw biochar-based slow-release rhizobium fertilizer and a preparation method thereof. The preparation method comprises the following steps: constructing hierarchical porous biochar MgWBC with magnesium-based active sites by adopting magnesium acetate impregnation and high-temperature pyrolysis; granular urea is used as a core, chitosan is used for packaging to realize directional loading and surface phase inversion film sealing of an MgWBC layer and rhizobium, and a composite structure with pH response capability is constructed. According to the method disclosed by the invention, the stress resistance and the directional colonization efficiency of the rhizobium are remarkably improved by utilizing the characteristic that the nitrogenase is activated by magnesium and the refuge effect of pores of the biochar. In a soybean-corn intercropping system, soybean nodulation and nitrogen fixation are enhanced and corn nutrition is met while nutrient slow release is realized through a synergistic mechanism of promoting nitrogen by magnesium. Experiments show that the yield of an intercropping system and the total nitrogen and effective magnesium level of soil can be remarkably improved, and the stability of soil aggregates is improved. The method is of great significance in promoting intercropping weight loss and efficiency improvement and cultivated land conservation.

The invention discloses bradyrhizobium yuanmingense ASHBWH-23-9 with functions of promoting growth and inhibiting aspergillus flavus and application of the bradyrhizobium yuanmingense ASHBWH-23-9, and the preservation number of the bradyrhizobium yuanmingense ASHBWH-23-9 is CCTCC (China Center for Type Culture Collection) M 20253053. The innovation of the invention lies in that the dual functions of generating cyclopentanone antibacterial volatile matters and efficiently symbiotically fixing nitrogen are integrated in the bradyrhizobium in the circular and clear garden for the first time. The molecular mechanism is that the generated volatile substance can accurately down-regulate the expression of a toxin synthesis key gene and a spore development core gene in aspergillus flavus. Compared with an existing microbial agent with a single function, nodulation and nitrogen fixation promotion and aspergillus flavus inhibition at the source can be synchronously realized at the rhizosphere of the peanuts through one-time inoculation, and particularly, aiming at the characteristics of overground flowering and underground fruiting of the peanuts, the microbial agent continuously plays a role in bacteriostasis and antipoison in soil in the pod development period.

The invention discloses an alfalfa stress-resistant high-yield breeding method based on a microbiome regulation and control technology, and belongs to the technical field of microbial breeding. According to the breeding method, compound fertilizer doped with a microbial agent is applied in a seedling stage, a branching stage and a squaring stage respectively, and the compound fertilizer comprises a substrate and the microbial agent, the microbial agent is prepared from sinorhizobium meliloti, bacillus stearothermophilus, peste des petits ruminants, bacillus licheniformis, azotobacter brown, bacillus siamensis and bacillus amyloliquefaciens; the substrate comprises at least two of cow dung, pig manure, bean bran, corn straw and wood chips. According to the breeding method, the new microbial composition is prepared for preparing the compound fertilizer, the nutrition absorption capacity of the alfalfa can be improved through a microbial regulation function, and then the stress resistance and yield of the alfalfa are improved.

This invention relates to the field of biotechnology, specifically to a compound microbial agent, preparation method, and application for promoting growth in moderately saline-alkali soils. The compound microbial agent comprises *Pseudomonas filamentosa* BG4, *Rhizobium sinense* DG1, *Arthrobacter* GCG2, *Pseudomonas* SG3, and *Arthrobacter spheroidae* GCG3. In mildly saline-alkali soils, compared to the control group, the compound microbial agent significantly increases the cumulative aboveground dry weight of corn and the yield per acre. Furthermore, its growth-promoting effect is superior in moderately saline-alkali soils compared to mildly saline-alkali soils. It can be applied to promote the growth of plants in saline-alkali soils and has broad application prospects.

The invention belongs to the technical field of fertilizers, and particularly relates to a preparation method of an organic compound and application of the organic compound in soil. The organic compound is prepared from the following raw materials: humic acid, maltodextrin, a microbial agent, polyacrylamide, oat bran, cow dung and brown algae. The microbial agent comprises nitrifying bacteria, rhizobium radiobium, bacillus licheniformis and bacillus megatherium. According to the invention, the raw materials are compounded for use, and the raw materials have a synergistic effect, so that the efficient organic compound capable of improving the soil fertility is provided. The raw materials of the organic compound are easy to obtain, the rice ear number is remarkably increased, the rice seed number per ear is increased, the rice dry grain weight is increased, the rice yield is effectively increased, the potato yield is effectively increased, and good economic benefits and social benefits are achieved.

The invention provides a saline-alkali soil chemical improvement and ecological restoration method which comprises the following steps: S1, performing rotary tillage on soil before planting, arranging according to wide and narrow rows, digging holes in the narrow rows, and applying a composite modifier; s2, coating alfalfa seeds with a suspension containing a rhizobium inoculant, ammonium molybdate and a water-retaining agent, and then sowing; s3, cutting in the initial flowering period of the alfalfa, reserving stubbles of 5-7cm, and crushing fresh grass to cover the wide-row earth surface; and S5, after cutting and before raining, spreading a compound microbial agent containing arbuscular mycorrhizal fungi and cellulose degrading bacteria, and after soil indexes reach the standard, turning and pressing alfalfa stubbles for crop rotation of food crops. Through combination of chemical improvement and ecological restoration, the water and fertilizer retention capacity of soil is improved, heavy metal pollution is relieved, and plant growth is promoted.

This invention belongs to the field of biotechnology, specifically relating to the plant growth-promoting properties of two alkali-producing strains, *Rhizobium miltiorrhiza* and *Pseudomonas mossae*, and their applications in soil cadmium fixation and fertilization. This invention provides a multifunctional alkali-producing bacterium, which is any one of the following strains: *Rhizobium miltiorrhiza* (… Rhizobium miluonense WW22, accession number: CGMCC No. 27718; *Pseudomonas molluscum* ( Pseudomonas mohnii WW2, accession number: CGMCC No. 27716. This invention also provides the use of the above-mentioned strain: cadmium fixation and fertilization. The strain of this invention can promote the reduction of cadmium availability in the soil and increase the nutrient content in the soil, providing a technical basis for ensuring the safe production and utilization of crops in cadmium-contaminated farmland, and has broad application prospects.

The invention discloses bradyrhizobium elkanii ASHLJNJ-23-2 which has the functions of efficiently promoting growth and inhibiting aspergillus flavus and application of the bradyrhizobium elkanii ASHLJNJ-23-2. The preservation number of the bradyrhizobium elkanii ASHLJNJ-23-2 is CCTCC M 2026227. According to the invention, the integration of the two functions of generating a characteristic antibacterial volatile matter of 2-tridecanone and efficiently symbiotic nitrogen fixation on a single strain is realized in the bradyrhizobium angularis for the first time. The 2-tridecanone volatile matter released by the strain can accurately down-regulate expression of toxin synthesis key genes and spore development core genes in aspergillus flavus, so that efficient bacteriostasis is realized on the molecular level. Through one-time inoculation, growth promotion, nitrogen fixation and source bacteriostasis can be synchronously realized at the rhizosphere ecological niche of the peanuts. Especially aiming at the biological characteristics of overground flowering and underground fruiting of peanuts, a lasting biological fumigation protection layer can be formed in a soil microenvironment in a legume development critical period.

The invention relates to the technical field of alfalfa planting, in particular to a method for improving the inoculation efficiency of alfalfa rhizobium, and adopts the following technical scheme that an alfalfa flavonoid crude extract diluent and a rhizobium suspension are mixed to form a composite inoculant which is used as a seed treatment agent and / or a soil improvement agent. The inoculation efficiency of the alfalfa rhizobium can be improved; the medicago sativa flavonoid crude extract is prepared from medicago sativa plants at the age of 2 months through grinding, alcohol extraction, ultrasonic treatment and centrifugation, drying, glycosylation removal, ethyl acetate treatment and extraction, and the medicago sativa flavonoid crude extract can improve the inoculation efficiency and nodulation capacity of rhizobium of medicago sativa.

The application provides a kind of based on ginkgo root secretion regulation alfalfa nodulation growth-promoting forest-grass interplanting method.The method makes full use of ginkgo under forest space, by land preparation and fertilization between forest rows, constructs high ridge square, selects high-quality alfalfa seed for strip sowing, combined with reasonable spacing and fixing seedling, cultivation and weeding and other field management measures, realize the normal growth and efficient utilization of alfalfa, provide a scientific and reasonable alfalfa interplanting mode.The application combines soil physical and chemical index determination, systematically evaluates forest soil before and after alfalfa planting, confirms that the method can effectively improve soil physical and chemical properties, promote ginkgo-alfalfa synergistic growth, has significant ecological benefits and application prospect.In addition, the application further verifies that ginkgo root secretion has obvious growth-promoting effect on alfalfa rhizobium, and can improve the expression level of alfalfa nod gene, enhance the number and quality of nodule, so as to improve the production performance of alfalfa and soil improvement effect.