455 results about "Rhizobium" patented technology

The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

The invention relates to a microbial inoculum for fermenting and producing organic fertilizers, comprising cellulomonas, bacillus, lactobacillus, methagen, thiobacillus, streptomyces, thermoactinomyces, thermomonospora, azotobacter, nitrosomonas, nitrobacter, rhizobium, aspergillus, yeast, penicillium, humicola, rhizopus and mycorrhizal fungi. In addition, the invention also relates to organic fertilizers fermented and produced by using the microbial inoculum and a method for fermentation and production.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

The invention relates to a microbial agent used for producing a soil modifying agent by fermentation, consisting of fiber monad, bacillus, lactobacillus, methanobacteria, thiobacillus, streptomycete, high-temperature actinomyces, high-temperature monad, azotobacteria, nitration monad, nitration bacilus, rhizobium, koji mold, leaven, blue mold, detritus mold, rhizopus as well as mycorrhizal fungi and substrate. In addition, the invention also relates to a soil modifying agent produced by the fermentation of the microbial agent, application thereof and the like.

The invention relates to compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil .In particular, the compound microbial agents comprises the bacillus megaterium microbial agent, the pseudomonas stutzeri microbial agent, the rhizobium radiobacter microbial agent, the candida tropicalis microbial agent and the lactobacillus delbrueckii microbial agent .The invention further comprises the bacterial manure containing the microbial agents, the preparation methods thereof and application thereof to restoration of the saline alkali soil .By means of the compound microbial agents, the bacterial manure, the preparation methods thereof and application thereof to restoration of the saline alkali soil, raw materials which pollute the environment easily or need to be disposed such as straw, excrements of livestocks, household garbage and sludge can be utilized sufficiently, in addition, the compound microbial bacterial manure capable of restoring the saline alkali soil can be prepared, thus the immune function and stress resistance of plants can be improved, the land salinization degree can be improved, and the purpose of achieving second ploughing of salinized soil is achieved.

The present invention relates to product(s) containing living microorganism(s) suitable for soil treatment, microorganisms multiplying under different climatic and natural circumstances, as well as procedures for the production of the products, and procedures for the treatment of the soil and plants with the products. More particularly, the invention relates to a procedure for preparing the products from any of the microorganisms specified below, or from the mixture thereof. Furthermore, the invention relates to a procedure for the creation of the cultures of the microorganisms to be used. The subject invention also pertains to the microorganisms themselves. More particularly, the invention relates to a procedure for the treatment of the soil and the plants with a product containing at least one of the microorganisms selected from Azospirillum brasilense ssp. SW51 (NCAIM /P/ B 001293), Azotobacter vinelandii ssp. M657 (NCAIM /P/B 001292), Pseudomonas fluorescens var. SW11 (NCAIM /P/ B 001296), Bacillus polymyxa var. SW17 (NCAIM /P/ B 001295), Bacillus megaterium var. M326 (NCAIM /P/ B 001291), Micrococcus roseus ssp. A21 (NCAIM /P/B 001294), Bradyrhizobium japonicum var. PH25 (NCAIM /P/ B 001302), and Streptomyces albus var. 0003 LP (NCAIM /P/ B 001301), and furthermore the products multiplying and existing in the environment of the plant in question, containing the listed microorganisms and their production.

The invention discloses a biomass charcoal soil conditioner. The soil conditioner comprises the following raw materials by weight: 40% to 50% of biomass charcoal, 20% to 30% of a functional microbial agent, 10% to 15% of desulfurized gypsum, 5% to 10% of calcium nitrate, 5% to 10% of ground phosphate rock and 1% to 2% of amino acid, wherein the functional microbial agent contains any two selected from the group consisting of bacillus subtilis, bacillus mucilaginosus, bacillus amyloliquefaciens, azotobacter, rhizobia and bacillus megatherium. The biomass charcoal soil conditioner provided by the invention can increase the water-retention rate and air permeability of soil, improve the content of organic matters in the soil, and regulate the pH value of the soil; meanwhile, the adoption of biological means to improve microbial environment of the soil is beneficial for promoting crop growth and high-efficiency nutrient utilization, increases the yield of crops, and improves the quality of the crops. The biomass charcoal soil conditioner provided by the invention effectively utilizes wastes, can improve the soil and enhance soil fertility, and specifically has significant improvement effect on saline alkali land.

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings/transplants of plants.

The invention relates to a multifunctional biological seed coating agent and a preparation method thereof. The multifunctional biological seed coating agent comprises auxiliary agents and beneficial microorganism, volume ratio thereof is 1:1-5, and effective bacterium content is not less than or equal to 10<8>cfu/ml. The auxiliary agent comprises one or various auxiliary agents in film former, dispersant, adhesion agent, protectant, nutritient, anti-freeze agent, preservative and colourant. The beneficial microorganism is selected from one or various rhizobium, azotobacter, pseudomonad, bacillus, K pneumoniae, Enterobacter cloacae and Raoulterlla planticola. The preparation method comprises the following steps: dissolving the polyvinyl alcohol and natural substance with water in a reaction kettle, mixing with the water dispersion liquid of the adhesion agent to prepare a film forming agent, and finally mixing and stirring mechanically with the beneficial microorganism fermentation liquor to obtain the finished product. The invention has the advantages of no residue, high efficiency, low toxicity, strong specificity, good film forming effect and convenient application, therefore, the multifunctional biological seed coating agent plays an significant role of preventing and controlling cotton seedling diseases as well as enhancing the cotton seedling growth under salt stress.

The regularly released granular organic fertilizer has manure as basic material and added nitrogen fertilizer, phosphate fertilizer, potash fertilizer, rhizobium and plant growth promoter and is produced into 0.0001-0.15 mm size coated agent. It is produced through a computerized control process, including disinfection with bacteriophage, disinfection with ozone generator, deodorizing, decoloring, centrifugal dewatering, freeze drying, sieving to eliminate impurity, mixing, coating with one layer of degradable plant casing, etc.

The invention relates to a bioflocculant fermentation method, in particular to a bioflocculant fermentation method with a mycelium pellet as a vector. The method solves the problems of high energy consumption, high production cost and unsuitability for large-scale industrialized fermentation production and the like existing in the existing bioflocculant fermentation method and comprises the following steps of: 1, mixing rhizobium radiobacter with bacillus sphaericus and then culturing so as to obtain flocculant-producing bacteria seed liquid; 2, culturing aspergillus niger to be a mature mycelium pellet; 3, mixing the mature mycelium pellet with the flocculant-producing bacteria seed liquid and then culturing so as to obtain a mixed mycelium pellet; 4, taking 24-hour as a fermentation period, and drawing out the mixed mycelium pellet after each fermentation period is finished and pouring the mixed mycelium pellet into fresh culture mediums for culturing; and 5, repetitively operating the step 4 for 30-35 times and namely completing. According to the bioflocculant fermentation method provided by the invention, on the premise of guaranteeing stable flocculating rate, seed liquid demand is reduced, production efficiency is increased and production energy consumption is reduced; moreover, the invention has great significance on large-scale industrialized production of bioflocculant.

The invention discloses an efficient cultivation method for increasing the yield and starch content of canna edulis. The method comprises steps of land selection, soil preparation, sowing, field management, pest disease and weed control, maturation, harvest, and seed reservation. The method comprises following operation steps: (1) land selection and soil preparation; (2) sowing including variety selection, sowing time selection, potato seed processing, base fertilizer application and planting mode selection; (3) filed management including seedling stage management, intertillage weeding and hilling, top application of fertilizer, water management and florescence management; (4) disease and pest control including disease control and pest control; (5) maturation and harvest; and (6) seed reservation. By employing the method, the canna edulis is high in yield and starch content, and problems that the canna edulis is low in mu yield and starch content are effectively solved. Through the interplanting of canna edulis and soybeans, the growth of weeds can be effectively controlled, soil is loosened, nitrogen is fixed through soybean rhizobium, the application amount of fertilizer can be reduced, green leaves and stems of the harvested soybeans can be returned to the field, the soil productivity can be increased, and finally the income of farmers can be increased.

An object of the present invention is to provide a novel ketose 3-epimerase, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for producing a ketose by using the enzyme. The present invention solves the above objects by providing a ketose 3-epimerase which is obtainable from a microorganism of the genus Rhizobium, a process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, and a process for converting D- or L-ketohexose into corresponding D- or L-ketohexose by epimerizing the hydroxyl group at the C-3 position of the D- or L-ketohexose; and D- or L-ketopentose into corresponding D- or L-ketopentose by epimerizing the hydroxyl group at the C-3 position of the D- or L-ketopentose; by using the enzyme.

The invention discloses an alfalfa seed obducens agent which is prepared from the following raw materials in percentage by weight: 1.5-5% of carboxymethylcellulose, 0.5-3% of rhizobium of alfalfa, 0.5-7% of biological humic acid, 0.2-3% of urea, 0.2-3% of monopotassium phosphate, 0.1-0.7% of ammonium molybdate, 0.1-0.5% of borax, 2-6% of sulfur powder, 0.15-0.7% of ferrous sulfate, 5-8% of sodium polyacrylate, 0.01-0.05% of indolebutyric acid, 0.01-0.05% of gibberellin, 0.01-0.05% of lysine acetate, 1.0-8.0% of thiram, 2.0-8.5% of thiophanate-methyl and the balance of alfalfa seeds. Aiming to the characteristics that alfalfa in the seedling stage is not saline-alkaline tolerant, draught tolerant, susceptible to diseases and the like, anti-salt and alkali, anti-drought and soil sterilizing factors are respectively added into the obducens agent disclosed by the invention, and moreover, the alfalfa seed obducens agent is obvious in production and application effects and is deeply welcomed by farmer households.

The invention discloses sinorhizobium SCAUs65 and application thereof. The strain is separated and purified from a fresh soybean nodulation; the strain is collected in the China center for type culture collection on March 14, 2014, the collection number of the strain is CCTCC NO: M2014084, and the strain is classified and named as sinorhizobium fredii. The strain is applied to the production of szechuan soybeans. The sinorhizobium SCAUs65 disclosed by the invention is an excellent broad-spectrum soybean rhizobium strain which has the advantages of high symbiotic nitrogen-fixing capacity, wide applicable range on szechuan soybean variety, inorganic phosphorus dissolving capacity, organic phosphorus dissolving capacity and potassium dissolving capacity and high stress resistance and has good matching affinity with szechuan main planted soybean variety. A soybean is increased in yield more than 38.9% by being inoculated with the sinorhizobium SCAUs65 without applying a nitrogen fertilizer in different ecological regions and achieves the difference to a significance level compared with a soybean which is not inoculated with the sinorhizobium SCAUs65.

The invention relates to a purification compound bacterium. The compound bacterium consists of 10-30% of beer yeast, 10-30% of plant lactic bacillus, 10-30% of lactobacillus acidophilus, 10-30% of bacillus subtilis, 5-15% of bacillus cereus and 5-15% of rhizobium radiobacter. Furthermore, the invention relates to a purification compound probiotic preparation and a preparation method thereof. The preparation method comprises the steps of inoculating the purification compound bacterium on a comprehensive liquid culture medium, and implementing multi-strain compound fermentation within a temperature range of 28-35 DEG C. By combining various aerobic, facultative and anaerobic strains, the purification compound probiotic preparation is prepared through an advanced industrial fermentation technology. The compound probiotic preparation has such advantages as being wide in application scope, high in treatment efficiency, free from secondary pollution, simple in equipment required, convenient for operation, low in operation cost, low in environmental load, and simple and convenient maintenance and management.

The invention relates to a rapeseed cake multi-effect fertilizer and a preparation method thereof. The fertilizer is prepared from the following raw materials in parts by weight: 200-250 parts of rapeseed cakes, 30-40 parts of pig manure, 30-40 parts of chicken manure, 30-40 parts of corn stalks, 30-40 parts of rice straws, 10-20 parts of calcium superphosphate, 10-20 parts of urea, 10-15 parts of monopotassium phosphate, 8-10 parts of ammonium phosphate, 8-10 parts of ferrous sulfite, 3-5 parts of zinc sulfate, 3-5 parts of boric acid, 10-20 parts of rhizobium powder, 8-15 parts of EM fungus, 3-4 parts of brown sugar and a proper amount of water. By adopting organic matter fermentation technology, the prepared rapeseed cake multi-effect fertilizer is not only rich in abundant organic matters and nutritional elements needed during growth of crops, is beneficial to the growth and fruiting of crops and improvement of yield of crops and content of nutritional substances, but also can effectively improve the morbidity resistance of crops, improve the structure, water retention and fertilizer retention and the like of soil, thus being a non-toxic multi-effect fertilizer with good ventilation property.

The invention discloses a broad bean and corn intercropping method and belongs to the field of crop cultivation methods. The method mainly relates to intercropping of the broad bean and the corn, wherein, emergence of seedlings of the broad bean is 10-20 days earlier than that of the corn, the broad bean and the corn are intercropped at a row ratio of 2:2 and at a row spacing from 28cm to 32cm. The method can help realize the purpose of simultaneously improving yield of the broad bean and biological nitrogen fixation amount, avoid the disadvantages of low yield of a leguminous crop which is cropped separately; secondly, the method can greatly reduce manually applied nitrogen fertilizer to save the plantation cost, and relieve the pressure on the environment due to nitrogen fertilizer abuse; furthermore, the method can adequately utilize indigenous rhizobium to avoid a degeneration problem of artificially inoculated strains, reduce operation steps such as inoculation and the like and save the inoculation cost.

The invention discloses a harden soil improvement method. The harden soil improvement method includes that using crop straw, peat soil and livestock manure to manufacture organic compound fertilizer, using lactic acid bacteria powder, nitrobacteria powder and rhizobium powder to manufacture mixed bacterial manure, mixing the organic compound fertilizer, mixed bacterial manure and microbial water retaining agent, and applying to the harden soil after mixing uniformly. The harden soil improvement method solves the problems that the moisture evaporation of the harden soil is fast, the harden soil is lack of organic substance, and the soil fertility is weak.

The invention discloses a bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim and an application of the bacterium. The bacterium capable of degrading pesticides chlorimuron-ethyl and carbendazim is rhizobium sp. LD1616 and the preservation number of the bacterium is CGMCC (China General Microbiological Culture Collection Center) No.7775. The rhizobium sp. LD1616 with the preservation of CGMCC NO.7775, disclosed by the invention, has the advantages that the degradation rate of the bacterium to chlorimuron-ethyl (45mg/L) is up to 71.56% in 7 days in an inorganic salt culture medium and the degradation rate of the bacterium to carbendazim (100mg/L) is up to 24.92% in 5 days, which indicates that the bacterium is capable of efficiently degrading the chlorimuron-ethyl and the carbendazim and has a broad application prospect in the remediation of soil polluted by residual pesticides chlorimuron-ethyl and carbendazim.

The invention relates to a (Bradyrhizobium sp.) LX-JX01 and application thereof. A preparation method of an inoculant for the (Bradyrhizobium sp.) LX-JX01 includes the steps of preparing a liquid DM culture medium, preparing a solid DM culture medium, activating a strain and culturing the rhizobium. The strain can be in nodulation and symbiosis with Dalbergia odorifera. When the inoculant for the Dalbergia odorifera rhizobium LX-JX01 is used for planting Dalbergia odorifera seedlings, nodulation number root nodules is increased by 3823% than a control group, fresh weight of the root nodules is increased by 1038%, dry weight of portions, above the ground, of plants is increased by 47.22%, and seedling height and plant stem diameter are increased by 1030% and 42.3% respectively, so that the inoculant can promote growing of Dalbergia odorifera. Consequently, the inoculant has wide application prospect in planting of artificial forest of Dalbergia odorifera.