73 results about "Rhizobium" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention belongs to the technical field of biology, and particularly relates to plant growth promoting performance of two alkali-producing strains, namely, Plutalophilous rhizobium and Pseudomonas moorsei, and application of the two alkali-producing strains to cadmium fixation and fertility increase of soil. The invention provides a multifunctional alcaligenes, which is any one of the following strains: Pentazobacterium mionense WW22, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 27718, and the preservation number of which is CGMCC No. 27718. The invention discloses pseudomonas mohnii WW2, and the preservation number of the pseudomonas mohnii WW2 is CGMCC (China General Microbiological Culture Collection Center) No.27716. The invention also provides application of the strain for fixing cadmium and increasing fertility. The strain disclosed by the invention can promote the reduction of the effectiveness of cadmium in soil and increase the nutrient content in the soil, provides a technical basis for ensuring the safe production and utilization of crops in a cadmium-polluted farmland, and has a wide application prospect.

The invention relates to a special organic compound fertilizer for alfalfa as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the special organic compound fertilizer for alfalfa comprises the following components in parts by weight: 50-60 parts of a basic organic fertilizer, 20-30 parts of a sodium humate-seaweed compound, 15-20 parts of modified phosphorus-potassium ore powder, 0.5-1 part of a phosphate-solubilizing bacterium agent and 0.5-1 part of an alfalfa rhizobium agent. On the basis of the existing basic organic fertilizer, the sodium humate-seaweed compound and the modified phosphorus-potassium ore powder loaded with the appropriate fungicide are mainly added, so that the yield of the alfalfa is remarkably increased, and the special organic compound fertilizer can be used as the special organic compound fertilizer for the alfalfa and is wide in application.

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIP5-GmPKM20 gene module in regulation and control of nodulation of leguminous plants, the GmNIP5-GmPKM20 gene module is one or more of GmNIP5 and genes interacting with the GmNIP5, by analyzing the influence of the transgenic GmNIP5 and the genes GmPKM20 interacting with the GmNIP5 on soybean nodulation, it is clear that the GmNIP5 promotes infection of nodule bacteria and formation of root nodules, and the GmNIP5-GmPKM20 gene module can be used for regulating and controlling nodulation of leguminous plants. GmPKM20 inhibits symbiotic nodulation signals of soybeans, and it is verified that GmPKM20 and GmPKM20 can directly interact with each other.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention relates to a vegetation recovery method for a power transmission project area of a loess hill in northwest of Shanxi. The method aims at solving the technical problems that in vegetation recovery of power transmission projects in loess hilly areas in the northwest of Shanxi Province, the power transmission projects cannot live, cannot grow well and cannot be stabilized. According to the technical scheme, the method comprises the steps that 1, sea-buckthorn, caragana microphylla and radix stemonae are used as main shrub layers, alfalfa and astragalus adsurgens are used as main herbal layers, and armeniaca sibirica and pyrus betulaefolia are used as restrictive arbor layers; (2) a tower footing platform is planted in a grid and strip mode in a mixed mode, a slope is planted in a contour strip mode, and a line channel is planted in a patch-shaped inlaying mode; and (3) carrying out intelligent drip irrigation on a soil improvement layer containing 5 + / -2% of biochar under soil moisture content threshold control, and inoculating with a rhizobium-phosphate solubilizing bacterium complex microbial inoculant. According to the method, the core problem that power transmission engineering vegetation in the loess hilly area in the northwest of Shanxi cannot live, cannot grow well and cannot stand is solved, the vegetation coverage can be increased to 60% or above within one year, a stable ecological community is formed after two years, and the water and soil loss amount is reduced by 50% or above compared with that of a traditional technology.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

The invention discloses a method for improving the quality of cultivated land through mixed sowing of perennial beans, grasses and forage grass. The method comprises the steps that deep ploughing and soil turning are conducted, land leveling is conducted, and organic fertilizer is applied to serve as base fertilizer; the method comprises the following steps: determining a mixing ratio of leguminous forage grass to gramineous forage grass of 3: 7-1: 1, and carrying out mixing pretreatment on the two forage grass seeds, namely preparing a nano titanium dioxide solution containing various nutrients and trace elements, screening stress-resistant domesticated rhizobium, disinfecting, soaking, coating and the like; reasonably topdressing in the forage grass growth period; when the pasture grows to a specific stage, the pasture is cut for 2-3 times every year; after 3-5 years of continuous utilization, crop rotation is carried out, and deep ploughing and fertilization are carried out again. According to the seed mixing pretreatment mode, the operation process is simplified, the cost is reduced, balanced nutrition supply is guaranteed, synchronous germination of seeds and coordinated growth of seedlings are promoted, and the fertilizer utilization rate is increased. The screened adversity-resistant domesticated rhizobium enhances the stress resistance of mixed sowing grassland, improves the yield and quality of alfalfa, and further improves the overall quality of mixed sowing pasture.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention discloses an astragalus sinicus breeding genetic data mining system based on bioinformatics, and particularly relates to the technical field of data mining. The method comprises the following steps: acquiring and synchronizing original sequencing data of an astragalus sinicus host genome and a rhizobium symbiotic microorganism genome to generate a standardized genetic information data set; the method comprises the following steps: identifying sequence cross contamination sites between a host genome and a symbiotic microorganism genome, and eliminating genetic information interference of a microorganism source, so as to obtain purified host genome data without microorganism interference; analyzing the contribution degree of the microbial genome to the target character of the astragalus sinicus to obtain a microbial effect weight; integrating the purified host genome data and the microbial effect weight, constructing an astragalus sinicus character association analysis model, and positioning character association sites; and finally, screening candidate breeding markers based on character associated site information to obtain a precise breeding genetic marker set. According to the method, the accuracy and efficiency of milk vetch breeding are improved.

The present application relates to "application of a compound in soybean cyst nematode chitinase inhibitor", belong to the technical field of leguminous plant symbiotic protection, the application of the compound and its pharmaceutically acceptable salt in soybean cyst nematode chitinase inhibitor, the present application is evaluated and researched through the inhibitory activity of compound by screening work, the inhibitor is screened finally, the inhibitor is likely to have good application prospect in controlling soybean cyst nematode disease aspect.The present application also provides a kind of chitinase inhibitor with the function of inhibiting the establishment of symbiosis of soybean cyst nematode to destroy, the number of nodule obtained by processing is flat with the number when only inoculating rhizobium, the inhibitory effect of Hg-CHI-1 on rhizobium nodule is completely restored, and it has good application prospect in the aspect of leguminous plant symbiotic protection.

The invention belongs to the technical field of fertilizers and relates to a compound fertilizer for black beans as well as a preparation method and application thereof. The compound fertilizer comprises a basic fertilizer, a microbial fertilizer and a trace element fertilizer, and a preparation method of the microbial fertilizer comprises the following steps: treating lignite with sophorolipid and trehalose lipid, treating turf with surfactin, mixing the treated lignite and turf, and then inoculating bacillus subtilis and rhizobium to obtain the microbial fertilizer. Through a unique dual mechanism of carrier synergism and fungicide function complementation, the dry matter cumulant and nitrogen cumulant of the overground part of the black bean are remarkably increased, and finally the yield and quality are remarkably improved.

The invention belongs to the technical field of camelina sativa planting, and particularly relates to a camelina sativa planting method suitable for being popularized in saline-alkali soil. The invention provides a planting method suitable for popularization of camelina sativa in saline-alkali soil. The planting method comprises the following steps: preparation before camelina sativa planting; seed variety selection; seed treatment: coating the camelina sativa seeds with a saline-alkaline tolerant rhizobium inoculant or a humate solution to obtain coated seeds; sowing the seeds; after sowing, cultivating to obtain camelina sativa plants. According to the planting method, the salt content of the surface layer of the soil can be remarkably reduced, the pH value of the soil is adjusted to be in the suitable range of 6.5-7.5, meanwhile, the germination rate, the seedling rate and the yield of the camelina sativa are increased, operation is easy and convenient, the cost is controllable, and the planting method is suitable for large-scale popularization in moderate and light saline-alkali areas and has wide application prospects. And a feasible technical scheme is provided for efficient utilization of saline-alkali land resources and regional expansion of the camelina sativa industry.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.

This invention discloses a genetically engineered bacterium and method for enhancing the denitrification efficiency of anaerobic ammonia oxidation at low temperatures. The genetically engineered bacterium is *Escherichia coli* expressing the cold shock protein gene *cspB*. The preparation method of this genetically engineered bacterium is as follows: (1) the cold shock protein gene *cspB* is ligated into the *pET-28a(+)* plasmid to obtain the *pET-28a(+)*-*cspB* plasmid; (2) the *pET-28a(+)*-*cspB* plasmid is introduced into *E. coli*, and after screening to obtain positive clones, it is cultured to obtain the genetically engineered bacterium. The method for enhancing the denitrification efficiency of anaerobic ammonia oxidation at low temperatures is as follows: the bacterial solution of the above-mentioned genetically engineered bacterium is mixed with the bacterial solution of *Rhizobium* to obtain a mixed bacterial solution; the mixed bacterial solution is placed at 2-8℃ for 4-8 hours for cold shock; the cold-shocked mixed bacterial solution is added to the anaerobic ammonia oxidation reactor. This invention can effectively improve the denitrification efficiency and operational stability of the anaerobic ammonia oxidation process at low temperatures.

This novel nonpathogenic bacterial strain exhibiting a controlling effect on crown gall disease in plants and tomato canker disease is the Allorhizobium vitis A3 strain (accession number: NITE BP-041154).

The present invention relates to the field of antimicrobial peptide technology, and in particular to an antimicrobial peptide with a double domain. Mesorhizobium A new AMP sequence with two domains was identified from the protein sequence of the plant (Eubacterium sp.). Independent studies of the β-sheet and α-helical domains revealed for the first time that both domains of a dual-domain antimicrobial peptide have the potential to independently exert antimicrobial activity. The β-sheet region has the potential to inhibit microbial film formation and eliminate mature microbial films, exhibiting particularly significant antimicrobial activity against fungi, but less potent antimicrobial activity against bacteria, potentially reducing damage to the intestinal microbiota. This invention provides new insights and experimental basis for the design of ultrashort antimicrobial peptides and holds broad application prospects.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.

The invention discloses a flora combination for in-situ enrichment of earthworms in soil, a culture medium and application of the flora combination, and the flora combination comprises Cupriavidus necator, a bacterial strain YTR-C1 with the preservation number of CGMCC27358, Sinorhizobium sp., a bacterial strain YTR-S1 with the preservation number of CGMCC20681, Mesorhizobium amorphae and a bacterial strain YTR-M3 with the preservation number of CGMCC17532, and the bacterial strain YTR-C1 with the preservation number of CGMCC27358, Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., Sinorhizobium sp., the flora composition is preserved in China General Microbiological Culture Collection Center on May 16th, 2023, September 18th, 2020 and April 9th, 2019, the flora composition and the culture medium can increase the number of the far-caffeilus in the red soil, field tests find that the effective phosphorus content and the phosphatase activity in the red soil are remarkably increased by adding the far-caffeilus, and the effective phosphorus content and the phosphatase activity of the red soil are remarkably increased by adding the far-caffeilus. The yield and the quality of the corn and the rape are improved.

The application provides application of RanGAP in regulation of symbiotic nodulation. A novel functional gene RanGAP (including RanGAP1 or RanGAP2) is disclosed, which encodes RanGAP protein with important biological function, and the protein is involved in symbiotic nitrogen fixation process of leguminous plants and rhizobium, and the application has significant meaning in genetic improvement of plant traits and soil improvement. An isolated promoter from RanGAP genome (upstream) is also disclosed, which has the function of driving gene-specific expression; more particularly, the promoter drives specific expression of the gene in root vascular bundle, lateral root / nodule primordium.

The application discloses a 5-aminoacetylpropionic acid-producing genetically recombined bacillus velezensis and application thereof, relates to the technical field of biotechnology and microbial metabolic engineering. The bacillus velezensis is obtained by integrating a hemA gene of Bradyrhizobium japonicum into the genome of bacillus velezensis R9, and the bacillus velezensis is obtained. The bacillus velezensis R9 has been preserved in the China Center for Type Culture Collection on July 5, 2023, and the preservation number is CCTCC NO: M 20231199. The strain can ferment to produce 17 mg / L of 5-aminoacetylpropionic acid in a 5-aminoacetylpropionic acid detection culture medium, and the concentration is more than 3 times higher than that of the bacillus velezensis R9, so the strain is an ideal engineering strain for producing 5-aminoacetylpropionic acid.

This invention relates to a compound microbial agent for the prevention and control of tobacco black shank disease, belonging to the field of biotechnology. The compound microbial agent comprises a suspension of *Pseudomonas aeruginosa* 232, *Bacillus cereus* 183, and *Mesophytes stolonifer* 161 in a volume ratio of 3:1:3, wherein the effective viable count in each of the aforementioned three bacterial suspensions is not less than 1 × 10⁻⁶. 9 CFU / mL. The compound biocontrol agent provided by this invention can effectively control tobacco black shank disease. Experimental verification shows that after 8 weeks of application of this agent to tobacco seedlings, the biocontrol effect against tobacco black shank disease can reach 33.34%.

The application discloses a Bradyrhizobium sp. and application thereof, and belongs to the technical field of nitrogen-fixing symbiotic microorganisms. The Bradyrhizobium sp. is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 27064. The strain has good growth-promoting characteristics, and has strong nitrogen tolerance and broad spectrum. In the case of reducing the nitrogen fertilizer application amount, the strain can significantly increase the number of rhizobia, the number of lateral branches, the length of main stems and the number of fruit needles of peanuts in the flowering stage, and improve the yield of peanuts in the maturing stage, thereby providing a strain source for obtaining rhizobium agents suitable for peanut planting of various varieties, providing a technical basis for reducing nitrogen fertilizer application and saving planting cost, providing an efficient and stable growth-promoting microbial germplasm resource, expanding oil crop symbiotic germplasm resources in China, and providing a basis for research and development of efficient and stable microbial fertilizers and application technologies.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The invention discloses a composite flora for relieving continuous cropping obstacles of soybeans and application of the composite flora, and belongs to the technical field of agricultural microorganisms. The composite flora comprises novosphingobacterium kaempferiae C6, sphingobacterium canadensis C14, variovorax sp. C16 and rhizobium oak C19; the preservation number of the sphingobium kaempferiae C6 is CGMCC (China General Microbiological Culture Collection Center) NO.35781, the preservation number of the sphingobium C14 is CGMCC NO.35782, the preservation number of the variovorax sp. C16 is CGMCC NO.35783, and the preservation number of the rhizobium oak C19 is CGMCC NO.35784. The invention further discloses a preparation method of the bacterial strain. The complex microbial inoculant prepared from the complex flora is used for relieving the soybean continuous cropping obstacle, and the result shows that the screened complex flora has a remarkable relieving effect on the soybean continuous cropping obstacle, can effectively improve the growth performance of soybeans and improve the antioxidant function of the soybeans, and has wide application value.

The application discloses a kind of compound microbial flora for relieving soybean continuous cropping obstacles and application thereof, belong to agricultural microbial technology field.The compound microbial flora includes novosphingobium kandensis C6, novosphingobium canadense C14, soil voracious bacteria C16 and rhizobium quercus C19;Novosphingobium kandensis C6 preservation number is CGMCC NO.35781, novosphingobium canadense C14 preservation number is CGMCC NO.35782, soil voracious bacteria C16 preservation number is CGMCC NO.35783, and rhizobium quercus C19 preservation number is CGMCC NO.35784.The compound microbial flora is used for preparing compound microbial agent for relieving soybean continuous cropping obstacles, and the results show that the compound microbial flora screened in the application has significant relieving effect on soybean continuous cropping obstacles, can effectively improve the growth performance of soybean, improve its antioxidant function, and has wide application value.

The invention discloses a microbial complex microbial inoculant for promoting the growth of leaf vegetables, which comprises bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum, and the viable count ratio of the bacillus subtilis, the rhodopseudomonas palustris and the bradyrhizobium japonicum is 3: 1: 1. The microbial complex microbial inoculant for promoting the growth of the leaf vegetables comprises bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum, and the viable count ratio of bacillus subtilis, rhodopseudomonas palustris and bradyrhizobium japonicum is 3: 1: 1. The preparation method of the microbial inoculum comprises the following steps: S1, respectively culturing the three bacteria to a logarithmic phase to obtain 109CFU / ml bacterial liquid; s2, mixing the fermentation broth according to a ratio, and ensuring that the viable count is accurately proportioned; s3, adding a carrier and auxiliary materials, and controlling the water content to be 35%; s4, drying at low temperature, protecting thallus activity, crushing, sieving and packaging to obtain a finished product. The three functional bacteria are scientifically proportioned, so that the synergistic growth promoting effect on the lettuce and the leaf mustard is realized, and the yield and the quality are remarkably improved.

The invention provides a water-soluble fertilizer and a compound fertilizer for promoting growth of alfalfa in saline-alkali soil as well as a preparation method and application of the water-soluble fertilizer and the compound fertilizer, and belongs to the technical field of fertilizers and preparation thereof. The water-soluble fertilizer comprises the following components in parts by weight: 30-40 parts of urea, 30-40 parts of ammonium sulfate, 8.5-9 parts of ammonium polyphosphate, 25-35 parts of potassium sulfate and 5-15 parts of humic acid. The compound fertilizer comprises the water-soluble fertilizer and 5-10 parts of a microbial agent. The microbial agent comprises rhizobium radiobacter, plant lactobacillus and halophilic alkali monad. The components and contents of the compound fertilizer meet the nutritional requirements of alfalfa in the growth and development period, and promote the growth of the alfalfa in the saline-alkali soil. The pH and the salt content of the saline-alkali soil can be reduced by adding the microbial agent, the growth environment of the alfalfa is improved by improving salinization of the saline-alkali soil, and the plant height and the yield of the alfalfa in the saline-alkali soil are obviously increased. The preparation method of the compound fertilizer is simple and fast.

The present application relates to the field of microbial technology, and discloses a new Aeschynomene indica rhizobium ZK1 T and application thereof, the new Aeschynomene indica rhizobium ZK1 T was preserved in the China General Microbiological Culture Collection Center on December 30, 2024, and the preservation number is CGMCC No.33228. The strain provided by the present application grows fast and has good stability, can grow and pass on the rhizobium culture medium YMA containing 2.0% NaCl at pH 4-10, and shows unique acid, alkali and salt nitrogen fixation capacity. The Aeschynomene indica rhizobium ZK1 T provided by the present application can degrade phosphorus elements on the organic phosphorus plate culture medium within 3d, and the phosphorus dissolution ring diameter is about 0.5 cm after about 5d. The Aeschynomene indica rhizobium ZK1 T provided by the present application can significantly improve the average plant height of different Aeschynomene indica variety materials by 106.81%, and the Aeschynomene indica rhizobium ZK1 T bacterial liquid can also improve the fresh weight of the aboveground part of the plant by 345.11%. The discovery of the strain provides strain resources and scientific support for the development and utilization of Aeschynomene indica rhizobium agent and the development of high-efficiency planting technology of marginal land Aeschynomene indica.