105 results about "Rhizobium" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention belongs to the technical field of biology, and particularly relates to plant growth promoting performance of two alkali-producing strains, namely, Plutalophilous rhizobium and Pseudomonas moorsei, and application of the two alkali-producing strains to cadmium fixation and fertility increase of soil. The invention provides a multifunctional alcaligenes, which is any one of the following strains: Pentazobacterium mionense WW22, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 27718, and the preservation number of which is CGMCC No. 27718. The invention discloses pseudomonas mohnii WW2, and the preservation number of the pseudomonas mohnii WW2 is CGMCC (China General Microbiological Culture Collection Center) No.27716. The invention also provides application of the strain for fixing cadmium and increasing fertility. The strain disclosed by the invention can promote the reduction of the effectiveness of cadmium in soil and increase the nutrient content in the soil, provides a technical basis for ensuring the safe production and utilization of crops in a cadmium-polluted farmland, and has a wide application prospect.

The invention relates to a special organic compound fertilizer for alfalfa as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the special organic compound fertilizer for alfalfa comprises the following components in parts by weight: 50-60 parts of a basic organic fertilizer, 20-30 parts of a sodium humate-seaweed compound, 15-20 parts of modified phosphorus-potassium ore powder, 0.5-1 part of a phosphate-solubilizing bacterium agent and 0.5-1 part of an alfalfa rhizobium agent. On the basis of the existing basic organic fertilizer, the sodium humate-seaweed compound and the modified phosphorus-potassium ore powder loaded with the appropriate fungicide are mainly added, so that the yield of the alfalfa is remarkably increased, and the special organic compound fertilizer can be used as the special organic compound fertilizer for the alfalfa and is wide in application.

The invention discloses a compound microorganism and application thereof to improvement of saline-alkali soil, and relates to the technical field of agriculture, the compound microorganism is obtained by compounding halophilic bacillus, rhodotorula mucilaginosa, alkali-resistant rhizobium, desulfurization vibrio and siliconizing bacteria, and the excellent saline-alkali soil improvement effect can be achieved. The functional strains cooperate with one another, have complementary advantages, and play a role in multiple aspects such as soil structure improvement, nutrient supply and absorption, salt and alkali relief, microbial community optimization and the like. The compound microbial preparation has an efficient synergistic mechanism, provides an innovative solution for solving the problem of saline-alkali soil, and shows huge potential in the aspects of promoting agricultural green development and ecological protection.

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention discloses an alkaline earth sinorhizobium sp. And application thereof, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 04, 2023, and the preservation number is CGMCC No.29223. The alkaline earth sinorhizobium sp. The strain has excellent stress resistance, can effectively promote nodulation and growth of soybeans in saline-alkali soil, achieves the effect of promoting growth and increasing yield of the soybeans, and thus has good practical application value.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

The invention belongs to the technical field of plant disease control, and particularly relates to preparation and application of diaphorina citri sourced antibacterial peptide DcAMP1. The amino acid sequence of the antibacterial peptide DcAMP1 is SEQ ID NO. 1, and the nucleotide sequence of the gene for coding the antibacterial peptide DcAMP1 is SEQ ID NO. 2. The prokaryotic expression plasmid is adopted to construct the recombinant plasmid containing the gene encoding the antibacterial peptide DcAMP1, prokaryotic expression of the antibacterial peptide DcAMP1 is achieved, and the method is simple and easy to implement. After being purified, the antibacterial peptide DcAMP1 obtained through prokaryotic expression has an obvious antibacterial effect on a liberobacter asiaticum related bacterium sinorhizobium fredii, and the titer of the liberobacter asiaticum in a sweet orange tree with the liberobacter asiaticum can be obviously reduced.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIP5-GmPKM20 gene module in regulation and control of nodulation of leguminous plants, the GmNIP5-GmPKM20 gene module is one or more of GmNIP5 and genes interacting with the GmNIP5, by analyzing the influence of the transgenic GmNIP5 and the genes GmPKM20 interacting with the GmNIP5 on soybean nodulation, it is clear that the GmNIP5 promotes infection of nodule bacteria and formation of root nodules, and the GmNIP5-GmPKM20 gene module can be used for regulating and controlling nodulation of leguminous plants. GmPKM20 inhibits symbiotic nodulation signals of soybeans, and it is verified that GmPKM20 and GmPKM20 can directly interact with each other.

The invention discloses a method for rapidly obtaining a transgenic raspberry plant. 3-6 mature leaves facing downwards from the top end of the raspberry are selected as receptor materials, the receptor materials are contacted with agrobacterium rhizogenes ATCC15834 for infection, the infected receptor materials are inserted into a soil matrix for co-culture, antibiotics are applied every week after co-culture, then calluses are formed at petioles, the calluses are differentiated into roots, and the roots sprout, so that the raspberry is obtained. And finally obtaining a transgenic raspberry plant. The method has the advantages of simplicity, short culture period and no need of tissue culture.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention relates to a vegetation recovery method for a power transmission project area of a loess hill in northwest of Shanxi. The method aims at solving the technical problems that in vegetation recovery of power transmission projects in loess hilly areas in the northwest of Shanxi Province, the power transmission projects cannot live, cannot grow well and cannot be stabilized. According to the technical scheme, the method comprises the steps that 1, sea-buckthorn, caragana microphylla and radix stemonae are used as main shrub layers, alfalfa and astragalus adsurgens are used as main herbal layers, and armeniaca sibirica and pyrus betulaefolia are used as restrictive arbor layers; (2) a tower footing platform is planted in a grid and strip mode in a mixed mode, a slope is planted in a contour strip mode, and a line channel is planted in a patch-shaped inlaying mode; and (3) carrying out intelligent drip irrigation on a soil improvement layer containing 5 + / -2% of biochar under soil moisture content threshold control, and inoculating with a rhizobium-phosphate solubilizing bacterium complex microbial inoculant. According to the method, the core problem that power transmission engineering vegetation in the loess hilly area in the northwest of Shanxi cannot live, cannot grow well and cannot stand is solved, the vegetation coverage can be increased to 60% or above within one year, a stable ecological community is formed after two years, and the water and soil loss amount is reduced by 50% or above compared with that of a traditional technology.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

The invention relates to a plateau soil remediation material and a preparation method thereof, and relates to the technical field of soil remediation materials, the plateau soil remediation material comprises the following components: a) 20-25 kg of a nano silicate material; b) 1000 kg of an organic fertilizer; c) 500 to 800 g of plant growth promoting hormone; and d) 1500ml to 2000ml of a mixture of plateau rhizobium and soil active bacteria. The components are mixed according to a specific proportion, and the soil remediation material which can comprehensively improve the plateau soil structure and promote plant growth, is environmentally friendly and adapts to the special plateau environment is prepared. The soil remediation material provided by the invention not only improves the fertility and biological activity of soil, but also promotes rapid growth and development of plants, and provides powerful support for soil remediation in plateau regions.

The invention discloses a method for improving the quality of cultivated land through mixed sowing of perennial beans, grasses and forage grass. The method comprises the steps that deep ploughing and soil turning are conducted, land leveling is conducted, and organic fertilizer is applied to serve as base fertilizer; the method comprises the following steps: determining a mixing ratio of leguminous forage grass to gramineous forage grass of 3: 7-1: 1, and carrying out mixing pretreatment on the two forage grass seeds, namely preparing a nano titanium dioxide solution containing various nutrients and trace elements, screening stress-resistant domesticated rhizobium, disinfecting, soaking, coating and the like; reasonably topdressing in the forage grass growth period; when the pasture grows to a specific stage, the pasture is cut for 2-3 times every year; after 3-5 years of continuous utilization, crop rotation is carried out, and deep ploughing and fertilization are carried out again. According to the seed mixing pretreatment mode, the operation process is simplified, the cost is reduced, balanced nutrition supply is guaranteed, synchronous germination of seeds and coordinated growth of seedlings are promoted, and the fertilizer utilization rate is increased. The screened adversity-resistant domesticated rhizobium enhances the stress resistance of mixed sowing grassland, improves the yield and quality of alfalfa, and further improves the overall quality of mixed sowing pasture.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention discloses an astragalus sinicus breeding genetic data mining system based on bioinformatics, and particularly relates to the technical field of data mining. The method comprises the following steps: acquiring and synchronizing original sequencing data of an astragalus sinicus host genome and a rhizobium symbiotic microorganism genome to generate a standardized genetic information data set; the method comprises the following steps: identifying sequence cross contamination sites between a host genome and a symbiotic microorganism genome, and eliminating genetic information interference of a microorganism source, so as to obtain purified host genome data without microorganism interference; analyzing the contribution degree of the microbial genome to the target character of the astragalus sinicus to obtain a microbial effect weight; integrating the purified host genome data and the microbial effect weight, constructing an astragalus sinicus character association analysis model, and positioning character association sites; and finally, screening candidate breeding markers based on character associated site information to obtain a precise breeding genetic marker set. According to the method, the accuracy and efficiency of milk vetch breeding are improved.

The present application relates to "application of a compound in soybean cyst nematode chitinase inhibitor", belong to the technical field of leguminous plant symbiotic protection, the application of the compound and its pharmaceutically acceptable salt in soybean cyst nematode chitinase inhibitor, the present application is evaluated and researched through the inhibitory activity of compound by screening work, the inhibitor is screened finally, the inhibitor is likely to have good application prospect in controlling soybean cyst nematode disease aspect.The present application also provides a kind of chitinase inhibitor with the function of inhibiting the establishment of symbiosis of soybean cyst nematode to destroy, the number of nodule obtained by processing is flat with the number when only inoculating rhizobium, the inhibitory effect of Hg-CHI-1 on rhizobium nodule is completely restored, and it has good application prospect in the aspect of leguminous plant symbiotic protection.

The invention belongs to the technical field of fertilizers and relates to a compound fertilizer for black beans as well as a preparation method and application thereof. The compound fertilizer comprises a basic fertilizer, a microbial fertilizer and a trace element fertilizer, and a preparation method of the microbial fertilizer comprises the following steps: treating lignite with sophorolipid and trehalose lipid, treating turf with surfactin, mixing the treated lignite and turf, and then inoculating bacillus subtilis and rhizobium to obtain the microbial fertilizer. Through a unique dual mechanism of carrier synergism and fungicide function complementation, the dry matter cumulant and nitrogen cumulant of the overground part of the black bean are remarkably increased, and finally the yield and quality are remarkably improved.

The invention belongs to the technical field of camelina sativa planting, and particularly relates to a camelina sativa planting method suitable for being popularized in saline-alkali soil. The invention provides a planting method suitable for popularization of camelina sativa in saline-alkali soil. The planting method comprises the following steps: preparation before camelina sativa planting; seed variety selection; seed treatment: coating the camelina sativa seeds with a saline-alkaline tolerant rhizobium inoculant or a humate solution to obtain coated seeds; sowing the seeds; after sowing, cultivating to obtain camelina sativa plants. According to the planting method, the salt content of the surface layer of the soil can be remarkably reduced, the pH value of the soil is adjusted to be in the suitable range of 6.5-7.5, meanwhile, the germination rate, the seedling rate and the yield of the camelina sativa are increased, operation is easy and convenient, the cost is controllable, and the planting method is suitable for large-scale popularization in moderate and light saline-alkali areas and has wide application prospects. And a feasible technical scheme is provided for efficient utilization of saline-alkali land resources and regional expansion of the camelina sativa industry.

The invention relates to the technical field of soil conditioners and applications thereof, and discloses a soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum and an application thereof in ploughing ability improvement, the soil carbon sequestration-pollution reduction-yield increase multi-effect synergistic microbial inoculum comprises an organic pollutant degradation functional flora, a soil carbon sequestration functional flora and a small molecular organic matter production functional flora; the organic pollutant degrading functional flora comprises efficient pollutant degrading bacteria; the soil carbon sequestration functional flora comprises ammonia oxidizing bacteria, ammonia oxidizing archaea, complete ammonia oxidizing bacteria, sulfate reducing bacteria, iron reducing bacteria, rhizobium and photosynthetic flora; the micromolecular organic matter production functional flora comprises heterotrophic nitrification-aerobic denitrification bacteria, zoogloea, phosphorus-accumulating bacteria and photosynthetic bacteria. According to the scheme, multiple florae are specifically arranged in the synergistic agent, and through the synergistic interaction effect of the florae, the effects of the synergistic agent in multiple fields of soil carbon sequestration, pollution reduction and yield increase are effectively improved.

The invention discloses a soil conditioner for improving crop stress resistance, and relates to the technical field of soil conditioners. The invention relates to a soil conditioner for improving the stress resistance of crops, which is used for improving the stress resistance of the crops. Comprising the following raw materials in parts by weight: 70-80 parts of fermented nutrient elements, 10-20 parts of urea, 15-30 parts of diammonium phosphate, 10-20 parts of potassium chloride, 30-50 parts of a trace element compound fertilizer, 40-60 parts of a humic acid fertilizer, 1-10 parts of charcoal, 10-20 parts of gibberellin, 10-20 parts of chitin, 10-20 parts of bacillus megatherium, 10-20 parts of arthrobacter chrysoflavus and 1-5 parts of Sinorhizobium freudeneri. 1 to 5 parts of bacillus licheniformis and 1 to 5 parts of acinetobacter.

This invention discloses a genetically engineered bacterium and method for enhancing the denitrification efficiency of anaerobic ammonia oxidation at low temperatures. The genetically engineered bacterium is *Escherichia coli* expressing the cold shock protein gene *cspB*. The preparation method of this genetically engineered bacterium is as follows: (1) the cold shock protein gene *cspB* is ligated into the *pET-28a(+)* plasmid to obtain the *pET-28a(+)*-*cspB* plasmid; (2) the *pET-28a(+)*-*cspB* plasmid is introduced into *E. coli*, and after screening to obtain positive clones, it is cultured to obtain the genetically engineered bacterium. The method for enhancing the denitrification efficiency of anaerobic ammonia oxidation at low temperatures is as follows: the bacterial solution of the above-mentioned genetically engineered bacterium is mixed with the bacterial solution of *Rhizobium* to obtain a mixed bacterial solution; the mixed bacterial solution is placed at 2-8℃ for 4-8 hours for cold shock; the cold-shocked mixed bacterial solution is added to the anaerobic ammonia oxidation reactor. This invention can effectively improve the denitrification efficiency and operational stability of the anaerobic ammonia oxidation process at low temperatures.

This novel nonpathogenic bacterial strain exhibiting a controlling effect on crown gall disease in plants and tomato canker disease is the Allorhizobium vitis A3 strain (accession number: NITE BP-041154).

The present invention relates to the field of antimicrobial peptide technology, and in particular to an antimicrobial peptide with a double domain. Mesorhizobium A new AMP sequence with two domains was identified from the protein sequence of the plant (Eubacterium sp.). Independent studies of the β-sheet and α-helical domains revealed for the first time that both domains of a dual-domain antimicrobial peptide have the potential to independently exert antimicrobial activity. The β-sheet region has the potential to inhibit microbial film formation and eliminate mature microbial films, exhibiting particularly significant antimicrobial activity against fungi, but less potent antimicrobial activity against bacteria, potentially reducing damage to the intestinal microbiota. This invention provides new insights and experimental basis for the design of ultrashort antimicrobial peptides and holds broad application prospects.

The invention discloses application of nitrogen-doped carbon dots as a nano fertilizer in promoting nitrogen capture of soybeans and green vegetables, and belongs to the technical field of nano agriculture. According to the invention, the nitrogen-doped carbon dots are used as a nano fertilizer to promote nitrogen capture of soybeans and green vegetables; according to the application, the nitrogen-doped carbon dots serve as a nano fertilizer and are prepared into an aqueous solution, then the aqueous solution is sprayed to the leaves of soybeans or green vegetables, controllable slow release of nitrogen compounds is achieved through the electrostatic interaction of high-activity functional groups such as amino and carboxyl on the surfaces of the carbon dots in combination with the optical excitation characteristic of the carbon dots, and nitrogen loss in soil is reduced; and by matching with the colonization of rhizobium of leguminous plants and the photosynthetic nitrogen fixation effect of the rhizobium, the activity of leaf metabolism key enzymes of vegetable leaf plants and the adsorption of root systems of green vegetables are met, so that the yield of soybeans and green vegetables is increased.

The present invention discloses a synthetic bacterial community for improving the quality and yield of simulated wild astragalus and its application, which belongs to the field of ecological planting of Chinese medicinal materials. The synthetic bacterial community is composed of 3 nitrogen-fixing bacteria, 2 phosphate-dissolving bacteria and 1 potassium-dissolving bacteria; the nitrogen-fixing bacteria are composed of Bacillus subtilis, Sinorhizobium and Rhizobium; the phosphate-dissolving bacteria are composed of Pseudomonas meningitidis and Pseudomonas putida; the potassium-dissolving bacteria is bidirectional Burkholderia. The synthetic bacterial community provided by the present invention is prepared into a bacterial agent, which is inoculated during the planting of astragalus, which significantly improves the yield and quality of astragalus, and the microbial community structure, soil nutrition and physical and chemical properties of the soil for planting astragalus are also significantly improved. The present invention provides a technical reference for standardizing the planting technology of Hunyuan astragalus, improving the level of planting technology, and improving the yield and quality of astragalus medicinal materials from the source.

This invention discloses a compound microbial inoculant and its application in licorice quality improvement in arid and saline-alkali land, belonging to the field of microbial technology and agricultural planting. The compound microbial inoculant includes *Arthrobacter spheroidae*, *Pseudomonas filamentosa*, *Rhizobium sinense* of alfalfa, and *Bacillus subtilis*; the viable count concentration of the compound microbial inoculant is 1-2 × 10⁻⁶. 10 CFU / mL. This compound microbial agent can significantly improve the yield and quality of licorice. Simultaneously, it can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium, and enhancing the activity of urease and alkaline phosphatase. This invention solves the problems of weak rhizosphere colonization and low component conversion rate of existing microbial agents, providing an innovative technical solution for the efficient cultivation of licorice in arid saline-alkali lands.