156 results about "Rhizobium" patented technology

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

The invention relates to the field of alfalfa planting, in particular to a synthetic flora for breaking dormancy of alfalfa seeds and promoting growth of seedlings and application of the synthetic flora. The invention provides a strain of sinorhizobium meliloti NFB5, the preservation number is CGMCC NO.33618, and the sinorhizobium meliloti NFB5 can break dormancy of alfalfa seeds and promote growth of alfalfa seedlings and root systems. According to the method, sinorhizobium meliloti NFB5 is compounded with one or two of screened salt-tolerant brevibacillus brevis EB3 and bacillus PS06 to obtain a synthetic flora, and alfalfa seeds are soaked in a bacterial solution containing the synthetic flora, so that the number of hard seeds can be remarkably reduced, the germination rate and germination potential of the seeds can be remarkably improved, and the germination rate of the seeds can be remarkably increased. Therefore, the synthetic flora can well break the dormancy of the alfalfa seeds, does not cause loss to the seeds, and does not reduce the quality of the seeds. In addition, the synthetic flora also has a significant growth promoting effect, and can significantly improve the plant height, root length, stem weight and root weight of seedlings.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention belongs to the technical field of biology, and particularly relates to plant growth promoting performance of two alkali-producing strains, namely, Plutalophilous rhizobium and Pseudomonas moorsei, and application of the two alkali-producing strains to cadmium fixation and fertility increase of soil. The invention provides a multifunctional alcaligenes, which is any one of the following strains: Pentazobacterium mionense WW22, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No. 27718, and the preservation number of which is CGMCC No. 27718. The invention discloses pseudomonas mohnii WW2, and the preservation number of the pseudomonas mohnii WW2 is CGMCC (China General Microbiological Culture Collection Center) No.27716. The invention also provides application of the strain for fixing cadmium and increasing fertility. The strain disclosed by the invention can promote the reduction of the effectiveness of cadmium in soil and increase the nutrient content in the soil, provides a technical basis for ensuring the safe production and utilization of crops in a cadmium-polluted farmland, and has a wide application prospect.

The invention relates to a special organic compound fertilizer for alfalfa as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the special organic compound fertilizer for alfalfa comprises the following components in parts by weight: 50-60 parts of a basic organic fertilizer, 20-30 parts of a sodium humate-seaweed compound, 15-20 parts of modified phosphorus-potassium ore powder, 0.5-1 part of a phosphate-solubilizing bacterium agent and 0.5-1 part of an alfalfa rhizobium agent. On the basis of the existing basic organic fertilizer, the sodium humate-seaweed compound and the modified phosphorus-potassium ore powder loaded with the appropriate fungicide are mainly added, so that the yield of the alfalfa is remarkably increased, and the special organic compound fertilizer can be used as the special organic compound fertilizer for the alfalfa and is wide in application.

The invention discloses a compound microorganism and application thereof to improvement of saline-alkali soil, and relates to the technical field of agriculture, the compound microorganism is obtained by compounding halophilic bacillus, rhodotorula mucilaginosa, alkali-resistant rhizobium, desulfurization vibrio and siliconizing bacteria, and the excellent saline-alkali soil improvement effect can be achieved. The functional strains cooperate with one another, have complementary advantages, and play a role in multiple aspects such as soil structure improvement, nutrient supply and absorption, salt and alkali relief, microbial community optimization and the like. The compound microbial preparation has an efficient synergistic mechanism, provides an innovative solution for solving the problem of saline-alkali soil, and shows huge potential in the aspects of promoting agricultural green development and ecological protection.

The invention discloses a biosynthetic bacterial cellulose-based solid electrolyte as well as a preparation method and application thereof, and belongs to the technical field of solid electrolytes. The solid electrolyte is prepared by taking modified bacterial cellulose prepared by fermenting acetobacter xylinum, rhizobium, pseudomonas, azospirillum, alcaligenes and other bacteria as a substrate, introducing a low-molecular-weight polymer to regulate and control the arrangement of cellulose molecular chains to serve as a conductive ion framework, and then compounding a conductive ion high-molecular-weight polymer and a lithium salt. According to the invention, cellulose molecular chains are modified by modified glucose, and a network structure is regulated and controlled by a low-molecular-weight polymer, so that the problems of small spacing, lack of ion coordination sites and weak ion transmission capability of pure cellulose molecular chains are effectively solved, the ionic conductivity of the prepared solid electrolyte can reach 10 <-4 > S cm <-1 >, the electrochemical window width reaches 5V, and the solid electrolyte has a good application prospect. The material can tolerate high critical current density, is suitable for various positive electrode materials, and is suitable for high-voltage and long-cycle-life energy storage devices.

The invention discloses a special bacterial fertilizer for sorghum and application of the special bacterial fertilizer in prevention and control of sorghum diseases, and belongs to the technical field of microbial fertilizers and agriculture. The special bacterial fertilizer for sorghum comprises the following components in parts by weight: 8-10 parts of a composite microbial agent, 40-50 parts of sorghum residues, 5-10 parts of bentonite, 1-2 parts of potassium humate, 1-2 parts of composite trace elements, 10-20 parts of sawdust and 10-20 parts of rice bran, wherein the complex microbial inoculant consists of a bacillus subtilis microbial inoculant, a rhizobium microbial inoculant, a bacillus velezensis microbial inoculant and a trichoderma harzianum microbial inoculant. The special bacterial fertilizer for sorghum provided by the invention contains rich microorganisms and nutrient substances, and can effectively prevent and control sorghum diseases and enhance the disease resistance of sorghum. The components such as microorganisms and organic acids in the special bacterial fertilizer for sorghum can promote the formation of soil organic matters, improve the soil structure, improve the soil quality and effectively improve the growth environment of sorghum.

The invention provides an intercropping method of tobacco and waltheria indica. The intercropping method comprises the following steps that 1, before tobacco and waltheria indica are planted, soil is deeply ploughed, a composite agent is sprayed, and then base fertilizer is applied; the composite agent is prepared from the following raw materials: ammonium sulfate, tea tree oil, rhizobium and a peanut vine extract; (2) tobacco seedlings are selected and transplanted in February to March, waltheria indica is planted between rows, and after planting is conducted for 2-3 weeks, compound fertilizer is applied between the rows of the tobacco seedlings and the waltheria indica; (3) when rainstorm weather occurs, spraying a complex microbial inoculant within 24 hours after rainstorm, and (4) managing until harvesting tobacco and waltheria indica. By the adoption of the intercropping method, nematodes in soil are inhibited, the tobacco and the waltheria indica are effectively improved, symbiosis and growth of the tobacco and the waltheria indica are promoted, and the harvesting yield per mu of the tobacco and the waltheria indica is increased.

The invention provides an acid-blocking and aluminum-controlling composite modifier and a preparation method and application thereof, the modifier is composed of microcrystalline activated minerals, a silicon-calcium material, a biomass alkaline material and a microbial agent, and the microbial agent comprises rhizobium, trichoderma harzianum and pseudomonas fluorescens. The specific raw material ratio can be adjusted according to different requirements, and the effect of materials with different ratios on improving the soil acidity and improving the crop yield and quality is researched by adopting field trials, so that an acid soil conditioner formula is formed; the preparation method comprises the steps of ultrafine grinding, microcrystal activation, hybridization, mixing and the like, the microcrystal activation adopts an activator solution for treatment, and an organic binder is added in the hybridization process to form an organic-inorganic hybrid structure. The composite modifier can effectively improve the physical action effect and the chemical reaction speed of the acid conditioner, and can be used for improving acid soil.

The invention provides sinorhizobium meliloti H9 as well as a high-density fermentation process and application thereof, and belongs to the technical field of microorganisms. The sinorhizobium meliloti is classified and named as sinorhizobium meliloti, and the sinorhizobium meliloti is preserved in the China General Microbiological Culture Collection Center on September 9, 2024, and the preservation number of the sinorhizobium meliloti is CGMCC (China General Microbiological Culture Collection Center) No. 31900. The sinorhizobium meliloti H9 provided by the invention is a high-efficiency phosphate-solubilizing bacterium and has strong stress resistance, and the sinorhizobium meliloti H9 and the high-density fermentation process thereof provided by the invention are beneficial to developing a novel efficient, broad-spectrum, green and environment-friendly phosphate-solubilizing bacterium biological agent.

The bacillus velezensis MY-8 disclosed by the invention has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various plant pathogenic bacteria including cabbage alternaria alternata, rhizopus arrhizus, cladosporium very fine, fusarium tripteroides, alternaria alternata, Columbia cladosporium, botryosphaeria dothidea, fusarium oxysporum, geotrichum candidum, colletotrichum gloeosporioides and A. arundinis, and the bacillus velezensis MY-8 has an obvious inhibition effect on various plant pathogenic bacteria such as bacillus velezensis MY-8. The compound has a remarkable control effect on downy mildew, is expected to be prepared into a microbial preparation for biological control, and can be used as a biopesticide, a bio-fertilizer, a plant growth promoter and the like. The bacillus velezensis MY-8 disclosed by the invention can also express a structural domain containing protein of DUF4393, so that the bacillus velezensis MY-8 has potential bacteriophage infection resistance.

The invention discloses an alkaline earth sinorhizobium sp. And application thereof, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 04, 2023, and the preservation number is CGMCC No.29223. The alkaline earth sinorhizobium sp. The strain has excellent stress resistance, can effectively promote nodulation and growth of soybeans in saline-alkali soil, achieves the effect of promoting growth and increasing yield of the soybeans, and thus has good practical application value.

The invention discloses a method for efficiently obtaining pepper transgenic hairy roots by using agrobacterium rhizogenes. Belongs to the technical field of plant genetic engineering. The method takes zanthoxylum bungeanum stem nodes as receptors, obtains hairy roots by agrobacterium rhizogenes mediation, and comprises the following steps: constructing an expression vector; performing agrobacterium tumefaciens transformation; preparing a transgenic receptor; carrying out explant infection; inducing hairy roots; transplanting the transgenic hairy root plant; and identifying the transgenic hairy roots. The method has the advantages of stable system, high conversion rate, simplicity in operation, workload saving and the like.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

The invention belongs to the technical field of plant disease control, and particularly relates to preparation and application of diaphorina citri sourced antibacterial peptide DcAMP1. The amino acid sequence of the antibacterial peptide DcAMP1 is SEQ ID NO. 1, and the nucleotide sequence of the gene for coding the antibacterial peptide DcAMP1 is SEQ ID NO. 2. The prokaryotic expression plasmid is adopted to construct the recombinant plasmid containing the gene encoding the antibacterial peptide DcAMP1, prokaryotic expression of the antibacterial peptide DcAMP1 is achieved, and the method is simple and easy to implement. After being purified, the antibacterial peptide DcAMP1 obtained through prokaryotic expression has an obvious antibacterial effect on a liberobacter asiaticum related bacterium sinorhizobium fredii, and the titer of the liberobacter asiaticum in a sweet orange tree with the liberobacter asiaticum can be obviously reduced.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIP5-GmPKM20 gene module in regulation and control of nodulation of leguminous plants, the GmNIP5-GmPKM20 gene module is one or more of GmNIP5 and genes interacting with the GmNIP5, by analyzing the influence of the transgenic GmNIP5 and the genes GmPKM20 interacting with the GmNIP5 on soybean nodulation, it is clear that the GmNIP5 promotes infection of nodule bacteria and formation of root nodules, and the GmNIP5-GmPKM20 gene module can be used for regulating and controlling nodulation of leguminous plants. GmPKM20 inhibits symbiotic nodulation signals of soybeans, and it is verified that GmPKM20 and GmPKM20 can directly interact with each other.

The invention discloses a method for efficiently obtaining hairy roots of transgenic roses without tissue culture. The method comprises the following steps: selecting a robust branch of a purple-branch rose seedling as an acceptor material, contacting a girdling wound with agrobacterium rhizogenes for infection, carrying out sealed culture on the infected acceptor material in a high-pressure propagation box, applying antibiotics every week after culturing for one week, and then forming positive callus at the girdling wound, after growing for two weeks, the positive callus is differentiated to form a positive root, and the hairy root of the transgenic rose is obtained. According to the established transgenic rose hairy root induction system, gene function verification can be efficiently and rapidly carried out in rose origin species, the rose molecular breeding process is accelerated, and a new method is provided for cultivation of new rose varieties.

The invention discloses a method for rapidly obtaining a transgenic raspberry plant. 3-6 mature leaves facing downwards from the top end of the raspberry are selected as receptor materials, the receptor materials are contacted with agrobacterium rhizogenes ATCC15834 for infection, the infected receptor materials are inserted into a soil matrix for co-culture, antibiotics are applied every week after co-culture, then calluses are formed at petioles, the calluses are differentiated into roots, and the roots sprout, so that the raspberry is obtained. And finally obtaining a transgenic raspberry plant. The method has the advantages of simplicity, short culture period and no need of tissue culture.

The invention relates to the technical field of ecology, and particularly discloses a soil matrix containing mud cakes, a preparation method of the soil matrix and application of the soil matrix in ecological restoration. The soil matrix comprises a solid mixture and water, wherein the solid mixture comprises 0.005%-0.01% of a growth-promoting nitrogen-fixing composite microbial agent, 20%-45% of an organic composite slow-release material, 25%-40% of a mud cake, 28%-42% of deep soil, 0.05%-0.1% of cationic polyacrylamide and 0.2%-0.4% of a vinyl acetate-vinyl versatate copolymer; the growth-promoting and nitrogen-fixing complex microbial inoculant comprises rhizobium, azotobacter chroococcum, bacillus megatherium and azospirillum brasilense; the organic composite slow-release material comprises an organic fertilizer, wood flour and a fertilizer. According to the method, a large number of mud cakes generated in projects such as hydroelectric development are fully utilized and developed into the matrix suitable for plant growth, a platform and a carrier are particularly provided for growth of plants on a slope, land resources are saved, and the method has the advantages of being high in operability, low in cost, good in ecological restoration effect and the like.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention discloses a method for LbbHLH36 gene transfer of Ningxia wolfberry mediated by Agrobacterium rhizogenes, and relates to the technical field of plant genetic engineering. The method for LbbHLH36 gene transfer of Ningxia wolfberry mediated by Agrobacterium rhizogenes disclosed by the invention is to scratch the hypocotyl of Ningxia wolfberry seedlings with a sterile blade, inoculate the incision with the activated Agrobacterium rhizogenes K599 bacterial solution carrying the target gene recombinant plasmid (35S::eGFP or pRI101-LbbHLH36-eGFP), and cultivate to obtain Ningxia wolfberry transgenic plants. The rolB, NeoR, eGFP, and LbbHLH36 genes in the induced transgenic fluorescent hairy roots of Ningxia wolfberry can all amplify bands, indicating that the above are all transgenic hairy roots induced by Agrobacterium rhizogenes, and it is fast and feasible to transfer the target gene into the root system of Ningxia wolfberry using this system.

The invention relates to a vegetation recovery method for a power transmission project area of a loess hill in northwest of Shanxi. The method aims at solving the technical problems that in vegetation recovery of power transmission projects in loess hilly areas in the northwest of Shanxi Province, the power transmission projects cannot live, cannot grow well and cannot be stabilized. According to the technical scheme, the method comprises the steps that 1, sea-buckthorn, caragana microphylla and radix stemonae are used as main shrub layers, alfalfa and astragalus adsurgens are used as main herbal layers, and armeniaca sibirica and pyrus betulaefolia are used as restrictive arbor layers; (2) a tower footing platform is planted in a grid and strip mode in a mixed mode, a slope is planted in a contour strip mode, and a line channel is planted in a patch-shaped inlaying mode; and (3) carrying out intelligent drip irrigation on a soil improvement layer containing 5 + / -2% of biochar under soil moisture content threshold control, and inoculating with a rhizobium-phosphate solubilizing bacterium complex microbial inoculant. According to the method, the core problem that power transmission engineering vegetation in the loess hilly area in the northwest of Shanxi cannot live, cannot grow well and cannot stand is solved, the vegetation coverage can be increased to 60% or above within one year, a stable ecological community is formed after two years, and the water and soil loss amount is reduced by 50% or above compared with that of a traditional technology.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

The invention relates to a plateau soil remediation material and a preparation method thereof, and relates to the technical field of soil remediation materials, the plateau soil remediation material comprises the following components: a) 20-25 kg of a nano silicate material; b) 1000 kg of an organic fertilizer; c) 500 to 800 g of plant growth promoting hormone; and d) 1500ml to 2000ml of a mixture of plateau rhizobium and soil active bacteria. The components are mixed according to a specific proportion, and the soil remediation material which can comprehensively improve the plateau soil structure and promote plant growth, is environmentally friendly and adapts to the special plateau environment is prepared. The soil remediation material provided by the invention not only improves the fertility and biological activity of soil, but also promotes rapid growth and development of plants, and provides powerful support for soil remediation in plateau regions.

The invention discloses a method for improving the quality of cultivated land through mixed sowing of perennial beans, grasses and forage grass. The method comprises the steps that deep ploughing and soil turning are conducted, land leveling is conducted, and organic fertilizer is applied to serve as base fertilizer; the method comprises the following steps: determining a mixing ratio of leguminous forage grass to gramineous forage grass of 3: 7-1: 1, and carrying out mixing pretreatment on the two forage grass seeds, namely preparing a nano titanium dioxide solution containing various nutrients and trace elements, screening stress-resistant domesticated rhizobium, disinfecting, soaking, coating and the like; reasonably topdressing in the forage grass growth period; when the pasture grows to a specific stage, the pasture is cut for 2-3 times every year; after 3-5 years of continuous utilization, crop rotation is carried out, and deep ploughing and fertilization are carried out again. According to the seed mixing pretreatment mode, the operation process is simplified, the cost is reduced, balanced nutrition supply is guaranteed, synchronous germination of seeds and coordinated growth of seedlings are promoted, and the fertilizer utilization rate is increased. The screened adversity-resistant domesticated rhizobium enhances the stress resistance of mixed sowing grassland, improves the yield and quality of alfalfa, and further improves the overall quality of mixed sowing pasture.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention discloses an astragalus sinicus breeding genetic data mining system based on bioinformatics, and particularly relates to the technical field of data mining. The method comprises the following steps: acquiring and synchronizing original sequencing data of an astragalus sinicus host genome and a rhizobium symbiotic microorganism genome to generate a standardized genetic information data set; the method comprises the following steps: identifying sequence cross contamination sites between a host genome and a symbiotic microorganism genome, and eliminating genetic information interference of a microorganism source, so as to obtain purified host genome data without microorganism interference; analyzing the contribution degree of the microbial genome to the target character of the astragalus sinicus to obtain a microbial effect weight; integrating the purified host genome data and the microbial effect weight, constructing an astragalus sinicus character association analysis model, and positioning character association sites; and finally, screening candidate breeding markers based on character associated site information to obtain a precise breeding genetic marker set. According to the method, the accuracy and efficiency of milk vetch breeding are improved.

The present application relates to "application of a compound in soybean cyst nematode chitinase inhibitor", belong to the technical field of leguminous plant symbiotic protection, the application of the compound and its pharmaceutically acceptable salt in soybean cyst nematode chitinase inhibitor, the present application is evaluated and researched through the inhibitory activity of compound by screening work, the inhibitor is screened finally, the inhibitor is likely to have good application prospect in controlling soybean cyst nematode disease aspect.The present application also provides a kind of chitinase inhibitor with the function of inhibiting the establishment of symbiosis of soybean cyst nematode to destroy, the number of nodule obtained by processing is flat with the number when only inoculating rhizobium, the inhibitory effect of Hg-CHI-1 on rhizobium nodule is completely restored, and it has good application prospect in the aspect of leguminous plant symbiotic protection.

The invention belongs to the technical field of biology, and particularly relates to a soybean hairy root genetic transformation method which comprises the following steps: taking soybean cotyledons as explants, infecting wounds of the explants by utilizing agrobacterium rhizogenes carrying reporter genes, transferring the infected explants into vermiculite, culturing, rooting, and screening positive roots. According to the method disclosed by the invention, a simple and efficient soybean hairy root genetic transformation system is established, tissue culture is not needed, and the problems of long transformation period, high cost and low transformation rate in the prior art are solved.