Efficient and safe lignocellulose degradation compound microbial agent and application thereof

Abstract

The invention discloses an efficient and safe lignocellulose degradation compound microbial agent and application thereof. The compound microbial agent is prepared from the following active ingredients: streptomyces microflavus, bacillus licheniformis, rhizobium and cellulomonas flavigena. The compound microbial flora is constructed by selecting safe and non-pathogenic microorganisms with lignocellulose degradation capability, and agricultural wastes such as straws, cottonseed hulls and the like can be effectively degraded. The complex microbial inoculant provided by the invention can be added into an edible fungus cultivation material to shorten the cultivation period, and can also be used in the aspects of preparation, composting and the like of a fermented straw feed. According to the method, efficient bioconversion of the crop waste rich in lignocellulose is facilitated, and development of circular agriculture is assisted.

Description

Technical Field

[0001] The invention relates to a highly efficient and safe lignocellulose degrading composite microbial agent and application thereof, belonging to the technical field of agricultural microorganisms. Background Art

[0002] Straw and forestry waste are rich in lignocellulose. They are not only agricultural waste but also important agricultural resources. Their effective utilization is of great significance for promoting low-carbon economic growth and responding to global climate change. At present, the utilization rate of straw and forestry waste in my country is steadily increasing. The application of straw fertilizer, feed, fuel, raw material and base material technologies has enabled the resource value of straw to be fully utilized. Edible fungi use agricultural waste rich in lignocellulose such as straw and sawdust as raw materials. In a suitable environment, they can germinate, differentiate primordia and produce mushrooms, turning low-quality agricultural waste into high-quality food. After the fruiting of mushrooms, the discarded bran can be further made into fertilizer or feed by adding bacteria. This is not only a clever combination of base material, fertilizer and feed, but also a win-win situation of ecological and economic benefits. However, since natural lignocellulose is composed of a strong structure of cellulose-hemicellulose-lignin network, it is difficult to be fully degraded, resulting in low biological efficiency of edible fungus cultivation materials and low utilization of bran, which restricts the development of the industry.

[0003] At present, most of the microorganisms commonly used to degrade agricultural waste are composed of fungi with strong lignocellulose degradation ability, such as Trichoderma, Aspergillus, Penicillium and white rot fungi, but the presence of Trichoderma, Penicillium and Aspergillus will seriously damage the fungus and growth of edible fungi, causing their yield and quality to decline, affecting economic benefits, and these pathogenic bacteria will also have safety hazards when used to make organic fertilizers and fermented straw feeds, so it is very important to select safe and non-pathogenic microorganisms. A single strain cannot effectively degrade lignocellulose due to the limited activity and types of lignocellulose degrading enzymes produced. Studies have shown that the composite microbial flora constructed by selecting multiple strains that are not antagonistic to each other has a better degradation effect than a single strain. The present invention aims to construct a green, safe and efficient microbial flora capable of degrading lignocellulose, which is used to efficiently degrade agricultural waste rich in lignocellulose. Summary of the invention

[0004] In view of the problem caused by the low degradation rate of existing agricultural waste, the present invention aims to provide a highly efficient and safe lignocellulose-degrading composite microbial agent and its application.

[0005] The composite microbial agent for degrading lignocellulose of the present invention is based on Streptomyces tenuiflavus ( 链霉菌属 微黄链霉菌 )、Bacillus licheniformis( 地衣芽孢杆菌 ), Rhizobium ( 根瘤菌属 ), Xanthomonas flavogenes (黄纤维单胞菌 ) composed of a complex microbial flora.

[0006] The components in the lignocellulose degradation composite microbial agent are composed of 30-60 parts of Streptomyces leucoderma fermentation liquid, 10-40 parts of Bacillus licheniformis fermentation liquid, 10-40 parts of Rhizobium fermentation liquid and 10-30 parts of Xanthomonas luteus fermentation liquid by volume.

[0007] More preferably, the fermentation liquid of Streptomyces tenuiflavus is 40 parts, the fermentation liquid of Bacillus licheniformis is 25 parts, the fermentation liquid of Rhizobium is 20 parts, and the fermentation liquid of Cellulomonas xanthogenes is 15 parts.

[0008] The composite microbial flora preparation can be obtained by mixing the fermentation liquid of Streptomyces tenuiflavus, the fermentation liquid of Bacillus licheniformis, the fermentation liquid of Rhizobium and the fermentation liquid of Xanthomonas fulvicidalis according to a certain proportion.

[0009] The Streptomyces tenuiflavus fermentation broth is prepared by a method comprising the following steps: After activating Streptomyces tenuifolius, a single clone was picked and inoculated into liquid culture medium at 28°C and 180 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 24-48 h. The number of viable bacteria CFU was (4.0-5.0)×10 10 The liquid culture medium formula of Streptomyces tenuifolius is: yeast extract powder 4.0 g / L, malt extract powder 10.0 g / L, glucose 4.0 g / L, pH 7.3.

[0010] The Bacillus licheniformis fermentation broth is prepared by a method comprising the following steps: After activating Bacillus licheniformis, a single clone was picked and inoculated into liquid culture medium at 30°C and 180 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 24-48 h. The number of viable bacteria CFU was (3.0-4.0)×10 10 The liquid culture medium formula of Bacillus licheniformis is: peptone 5.0 g / L, beef extract powder 3.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0011] The rhizobium fermentation broth is prepared by a method comprising the following steps: After the rhizobium is activated, a single clone is picked and inoculated into liquid culture medium at 30°C and 180 rpm. After overnight culture, it is transferred to a fermenter containing liquid culture medium for expansion culture for 48-72 h. The number of viable bacteria CFU is (3.0-4.0)×10 10 The liquid culture medium formula of rhizobia is: peptone 5.0 g / L, beef extract powder 3.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0012] The Xanthocellomonas fermentation broth is prepared by a method comprising the following steps: After the activation of Xanthomonas flavogenes, a single clone was selected and inoculated into liquid culture medium at 37°C and 150 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 24-48 h. The CFU count of viable bacteria was (6.0-7.0)×10 10 The liquid culture medium formula of Xanthomonas flavogenes is: tryptone 10.0 g / L, yeast extract 5.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0013] The invention discloses an application of the composite microbial agent in the degradation of agricultural wastes rich in lignocellulose.

[0014] The agricultural waste rich in lignocellulose includes one or more of corn straw, wheat straw, rice straw, rape straw, cottonseed hulls, wood chips and the like.

[0015] The specific steps include: Step 1: Dry the agricultural waste rich in lignocellulose at 60°C to constant weight, then grind it and pass it through a 40-mesh sieve. Mix the dried agricultural waste with water and add it to a culture dish. Sterilize it at 121°C for 30 minutes to obtain a solid culture medium of agricultural waste for later use.

[0016] Step 2: Add the composite microbial agent to the agricultural waste solid culture medium obtained in step 1, stir evenly, culture at 25-30° C. for 10-20 days, and observe the degradation changes of the culture medium.

[0017] In step 1, the mass ratio of the dried agricultural waste to water is 1:2.5.

[0018] In step 2, the inoculation amount of the lignocellulose-degrading composite microbial agent is 3-5%.

[0019] After the fermentation, the samples were taken out and dried at 60°C to constant weight. The degradation rate of agricultural waste was calculated, and an equal amount of sterile water was inoculated as the blank control group.

[0020] The agricultural waste degradation rate calculation formula of the present invention is: Degradation rate (%) = (R0 - R) × 100%; Among them, R0 is the dry weight of agricultural waste before degradation, and R is the dry weight of agricultural waste after degradation.

[0021] Compared with the prior art, the present invention uses Streptomyces leucoflavus, Bacillus licheniformis, Rhizobium and Xanthocellomonas, which are safe, non-pathogenic, non-antagonistic to edible fungi and have the function of degrading lignocellulose. They are used to construct a composite microbial agent in an optimal ratio, which can effectively degrade agricultural waste rich in lignocellulose. The present invention can be added to edible fungus cultivation materials to promote the degradation of cultivation materials and shorten the cultivation period. It can also be used in the preparation and composting of fermented straw feed, which is of great significance to the comprehensive utilization of agricultural waste and the development of agricultural ecological circulation mode. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of the strain antagonism test in Example 1.

[0023] Figure 2 The results of the degradation rate of rice straw by the composite microbial agent and single bacteria in Example 4 are shown in FIG.

[0024] Figure 3 The results of cottonseed hull degradation rate by composite microbial agent and single bacteria in Example 5 are shown in FIG. DETAILED DESCRIPTION

[0025] Various specific embodiments of the present invention are described in detail below. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features and embodiments of the present invention.

[0026] Microbial materials: Streptomyces leucoderma described in the examples ( 微黄链霉菌 ) CICC23627, Bacillus licheniformis ( 地衣芽孢杆菌 ) CICC 10037 and Rhizobium ( 根瘤菌 属 ) CICC 10886 were purchased from China Industrial Microbiological Culture Collection Center, and the Xanthocellomonas ( 黄纤维单胞菌 ) GMCC1.12219 was purchased from China General Microbiological Culture Collection Center. Example 1: Antagonism test between strains

[0027] Streptomyces tenuiflavus, Bacillus licheniformis, Rhizobium, and Xanthomonas luteus were inoculated into LB medium by two-by-two streaking method. Figure 1 As shown (the dark line is the first strain, and the light line is the second strain), the strains were inverted and cultured at a constant temperature of 28°C for 2-3 days, and the crossover of the strains was observed to see if there were obvious faults and hydrolysis zones to determine the antagonistic effect. If there were obvious faults and hydrolysis zones, it meant that the two strains had produced an inhibition zone and had an antagonistic effect; otherwise, it meant that the two strains had no antagonistic reaction and could be combined. The results are shown in Table 1 below.

[0028] Example 2: Fermentation preparation of composite microbial agent for lignocellulose degradation

[0029] 1. After activating Streptomyces leucodermi, a single clone was selected and inoculated into liquid culture medium at 28°C and 180 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 36 h. The number of viable bacteria CFU was 4.5×10 10 The liquid culture medium formula of Streptomyces tenuifolius is: yeast extract powder 4.0 g / L, malt extract powder 10.0 g / L, glucose 4.0 g / L, pH 7.3.

[0030] 2. After activating Bacillus licheniformis, a single clone was selected and inoculated into liquid culture medium at 30°C and 180 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 24 hours. The number of viable bacteria CFU was 3.5×10 10 The liquid culture medium formula of Bacillus licheniformis is: peptone 5.0 g / L, beef extract powder 3.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0031] 3. After the rhizobium is activated, a single clone is selected and inoculated into liquid culture medium at 30°C and 180 rpm. After overnight culture, it is transferred to a fermenter containing liquid culture medium for expansion culture for 48 h. The number of viable bacteria CFU is 4.0×10 10 The liquid culture medium formula of rhizobia is: peptone 5.0 g / L, beef extract powder 3.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0032] 4. After activating the yellow cellulomonas, a single clone was selected and inoculated into liquid culture medium at 37°C and 150 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 24 h. The number of viable bacteria CFU was 6.0×10 10 The liquid culture medium formula of Xanthomonas flavogenes is: tryptone 10.0 g / L, yeast extract 5.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0033] 5. Take 40 parts of fermentation broth of Streptomyces tenuifolius, 25 parts of fermentation broth of Bacillus licheniformis, 20 parts of Rhizobium, and 15 parts of fermentation broth of Cellulomonas xanthogenes and mix them to obtain a composite microbial flora preparation. Example 3: Fermentation preparation of composite microbial agent for lignocellulose degradation

[0034] 1. After activating Streptomyces leucodermi, a single clone was selected and inoculated into liquid culture medium at 28°C and 180 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 48 h. The number of viable bacteria CFU was 5.0×1010 The liquid culture medium formula of Streptomyces tenuifolius is: yeast extract powder 4.0 g / L, malt extract powder 10.0 g / L, glucose 4.0 g / L, pH 7.3.

[0035] 2. After activating Bacillus licheniformis, a single clone was selected and inoculated into liquid culture medium at 30°C and 180 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 36 h. The number of viable bacteria CFU was 3.8×10 10 The liquid culture medium formula of Bacillus licheniformis is: peptone 5.0 g / L, beef extract powder 3.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0036] 3. After the rhizobium is activated, a single clone is selected and inoculated into liquid culture medium at 30°C and 180 rpm. After overnight culture, it is transferred to a fermenter containing liquid culture medium for expansion culture for 48 h. The number of viable bacteria CFU is 4.0×10 10 The liquid culture medium formula of rhizobia is: peptone 5.0 g / L, beef extract powder 3.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0037] 4. After activating the yellow fiber monocytogenes, a single clone was selected and inoculated into liquid culture medium at 37°C and 150 rpm. After overnight culture, it was transferred to a fermenter containing liquid culture medium for expansion culture for 36 h. The number of viable bacteria CFU was 6.5×10 10 The liquid culture medium formula of Xanthomonas flavogenes is: tryptone 10.0 g / L, yeast extract 5.0 g / L, NaCl 5.0 g / L, pH 7.0.

[0038] 5. Take 35 parts of fermentation broth of Streptomyces tenuifolius, 30 parts of fermentation broth of Bacillus licheniformis, 15 parts of Rhizobium, and 20 parts of fermentation broth of Cellulomonas xanthogenes and mix them to obtain a composite microbial flora preparation. Example 4: Degradation of rice straw by a lignocellulose-degrading composite microbial agent

[0039] In this embodiment, the application of the composite microbial agent is to degrade rice straw as an example. Streptomyces leucoderma, Bacillus licheniformis, Rhizobium, and Xanthomonas luteus are fermented in their respective fermentation systems to achieve a CFU count of 5.0×10 10 , the CFU count of Bacillus licheniformis fermentation broth was 4.0×10 10 , the CFU of Rhizobium fermentation broth was 4.0×10 10 , the CFU count of Flavomonas flavobacterium fermentation broth was 7.0×10 10 Collect the fermentation broth of the bacteria.

[0040] In one embodiment A, 40 parts of fermentation broth of Streptomyces tenuiflavus, 25 parts of fermentation broth of Bacillus licheniformis, 20 parts of fermentation broth of Rhizobium, and 15 parts of fermentation broth of Cellulomonas xanthogenes are mixed to obtain a composite microbial flora preparation.

[0041] The rice straw was placed at 60°C and dried to constant weight, then crushed and passed through a 40-mesh sieve. 100 g of the dried rice straw was mixed with water at a mass ratio of 1:2.5 and added to a culture dish, sterilized at 121°C for 30 minutes to obtain a rice straw solid culture medium. The lignocellulose degradation composite microbial agent, Streptomyces flavosus fermentation broth (Sm), Bacillus licheniformis fermentation broth (Bl), Rhizobium fermentation broth (Rs) and Xanthomonas flavosus fermentation broth (Cf) were added to the rice straw solid culture medium at an inoculation rate of 5%, stirred evenly, and cultured at 30°C for 20 days to observe the degradation changes of the culture material. At the same time, an equal amount of sterile water was inoculated as a blank control group. After the fermentation was completed, the rice straw was rinsed with clean water 3 times, dried at 60°C to constant weight, and the rice straw degradation rate was calculated.

[0042] In one embodiment B, 30 parts of fermentation broth of Streptomyces tenuiflavus, 25 parts of fermentation broth of Bacillus licheniformis, 20 parts of fermentation broth of Rhizobium, and 25 parts of fermentation broth of Cellulomonas xanthogenes are mixed to obtain a composite microbial flora preparation.

[0043] The rice straw was placed at 60°C and dried to constant weight, then crushed and passed through a 40-mesh sieve. The dried rice straw was mixed with water at a mass ratio of 1:2.5 and added to a culture dish, sterilized at 121°C for 30 minutes to obtain a rice straw solid culture medium. The lignocellulose degradation composite microbial agent, Streptomyces flavosum fermentation broth (Sm), Bacillus licheniformis fermentation broth (Bl), Rhizobium fermentation broth (Rs) and Xanthomonas luteus fermentation broth (Cf) were added to the rice straw solid culture medium at an inoculation rate of 5%, stirred evenly, and cultured at 30°C for 20 days to observe the degradation changes of the cultivation material. At the same time, an equal amount of sterile water was inoculated as a blank control group. After the fermentation was completed, the rice straw was rinsed with clean water 3 times, dried at 60°C to constant weight, and the rice straw degradation rate was calculated.

[0044] In one embodiment C, 25 parts of fermentation broth of Streptomyces tenuiflavus, 25 parts of fermentation broth of Bacillus licheniformis, 25 parts of fermentation broth of Rhizobium, and 25 parts of fermentation broth of Cellulomonas xanthogenes are mixed to obtain a composite microbial flora preparation.

[0045] The rice straw was placed at 60°C and dried to constant weight, then crushed and passed through a 40-mesh sieve. The dried rice straw was mixed with water at a mass ratio of 1:2.5 and added to a culture dish, sterilized at 121°C for 30 minutes to obtain a rice straw solid culture medium. The lignocellulose degradation composite microbial agent, Streptomyces flavosum fermentation broth (Sm), Bacillus licheniformis fermentation broth (Bl), Rhizobium fermentation broth (Rs) and Xanthomonas luteus fermentation broth (Cf) were added to the rice straw solid culture medium at an inoculation rate of 5%, stirred evenly, and cultured at 30°C for 20 days to observe the degradation changes of the cultivation material. At the same time, an equal amount of sterile water was inoculated as a blank control group. After the fermentation was completed, the rice straw was rinsed with clean water 3 times, dried at 60°C to constant weight, and the rice straw degradation rate was calculated.

[0046] Rice straw degradation results Figure 2 As shown, after 20 days of degradation, the degradation rates of rice straw in Examples A, B, and C were 52.87%, 46.60%, and 43.47%, respectively, which were significantly higher than the degradation rates of the four single bacteria. The degradation efficiency of rice straw was highest when the fermentation liquid of Streptomyces tenuiflavos, Bacillus licheniformis, Rhizobium, and Xanthomonas luteus was in a volume ratio of 40:25:20:15. Example 5: Degradation of cottonseed hulls by a lignocellulose-degrading composite microbial agent

[0047] In this embodiment, the application of the composite microbial agent is to degrade cottonseed hulls as an example. Streptomyces leucoderma, Bacillus licheniformis, Rhizobium, and Xanthomonas luteus are fermented in their respective fermentation systems to achieve a CFU count of 5.0×10 10 , the CFU count of Bacillus licheniformis fermentation broth was 4.0×10 10 , the CFU of Rhizobium fermentation broth was 4.0×10 10 , the CFU count of Flavomonas flavobacterium fermentation broth was 7.0×10 10 Collect the fermentation broth of the bacteria.

[0048] In one embodiment A, 40 parts of fermentation broth of Streptomyces tenuiflavus, 25 parts of fermentation broth of Bacillus licheniformis, 20 parts of fermentation broth of Rhizobium, and 15 parts of fermentation broth of Cellulomonas xanthogenes are mixed to obtain a composite microbial flora preparation.

[0049] The cottonseed hulls were dried at 60°C until constant weight, then crushed and passed through a 40-mesh sieve. 100 g of the dried cottonseed hulls were mixed with water at a mass ratio of 1:2.5 and added to a culture dish. The mixture was sterilized at 121°C for 30 minutes to obtain a cottonseed hull solid culture medium. The lignocellulose-degrading composite microbial agent, Streptomyces pyogenes fermentation broth (Sm), Bacillus licheniformis fermentation broth (Bl), Rhizobium fermentation broth (Rs) and Xanthomonas chlororaphis fermentation broth (Cf) were added to the cottonseed hull solid culture medium at an inoculation rate of 5%, stirred evenly, and cultured at 30°C for 20 days to observe the degradation changes of the culture material. At the same time, an equal amount of sterile water was inoculated as a blank control group. After the fermentation was completed, the cottonseed hulls were rinsed with clean water 3 times, dried at 60°C to constant weight, and the degradation rate of the cottonseed hulls was calculated.

[0050] In one embodiment B, 30 parts of fermentation broth of Streptomyces tenuiflavus, 25 parts of fermentation broth of Bacillus licheniformis, 20 parts of fermentation broth of Rhizobium, and 25 parts of fermentation broth of Cellulomonas xanthogenes are mixed to obtain a composite microbial flora preparation.

[0051] The cottonseed hulls were placed at 60°C and dried to constant weight, then crushed and passed through a 40-mesh sieve. The dried cottonseed hulls were mixed with water in a mass ratio of 1:2.5 and added to a culture dish, sterilized at 121°C for 30 minutes to obtain a cottonseed hull solid culture medium. The lignocellulose-degrading composite microbial agent, Streptomyces flavosum fermentation broth (Sm), Bacillus licheniformis fermentation broth (Bl), Rhizobium fermentation broth (Rs) and yellow fiber-producing monosaccharides fermentation broth (Cf) were added to the cottonseed hull solid culture medium at an inoculation rate of 5%, stirred evenly, and cultured at 30°C for 20 days to observe the degradation changes of the cultivation material. At the same time, an equal amount of sterile water was inoculated as a blank control group. After the fermentation was completed, the cottonseed hulls were rinsed with clean water 3 times, dried at 60°C to constant weight, and the degradation rate of the cottonseed hulls was calculated.

[0052] In one embodiment C, 25 parts of fermentation broth of Streptomyces tenuiflavus, 25 parts of fermentation broth of Bacillus licheniformis, 25 parts of fermentation broth of Rhizobium, and 25 parts of fermentation broth of Cellulomonas xanthogenes are mixed to obtain a composite microbial flora preparation.

[0053] The cottonseed hulls were placed at 60°C and dried to constant weight, then crushed and passed through a 40-mesh sieve. The dried cottonseed hulls were mixed with water in a mass ratio of 1:2.5 and added to a culture dish, sterilized at 121°C for 30 minutes to obtain a cottonseed hull solid culture medium. The lignocellulose-degrading composite microbial agent, Streptomyces flavosum fermentation broth (Sm), Bacillus licheniformis fermentation broth (Bl), Rhizobium fermentation broth (Rs) and yellow fiber-producing monosaccharides fermentation broth (Cf) were added to the cottonseed hull solid culture medium at an inoculation rate of 5%, stirred evenly, and cultured at 30°C for 20 days to observe the degradation changes of the cultivation material. At the same time, an equal amount of sterile water was inoculated as a blank control group. After the fermentation was completed, the cottonseed hulls were rinsed with clean water 3 times, dried at 60°C to constant weight, and the degradation rate of the cottonseed hulls was calculated.

[0054] Cottonseed hull degradation results Figure 3 As shown, after 20 days of degradation, the degradation rates of cottonseed hulls of Examples A, B, and C were 43.80%, 40.80%, and 38.07%, respectively, which were significantly higher than the degradation rates of the four single bacteria. The degradation efficiency of cottonseed hulls was highest when the fermentation broth of Streptomyces tenuiflavos, Bacillus licheniformis, Rhizobium, and Xanthomonas luteus was in a volume ratio of 40:25:20:15.

Claims

1. An efficient and safe composite microbial agent for degrading lignocellulose, characterized by: The lignocellulose degradation composite microbial agent is a composite microbial flora composed of Streptomyces tenuiflavus, Bacillus licheniformis, Rhizobium and Xanthocellomonas.

2. The lignocellulose-degrading composite microbial agent according to claim 1, characterized in that The components by volume are as follows: 30-60 parts of Streptomyces tenuiflavus fermentation broth, 10-40 parts of Bacillus licheniformis fermentation broth, 10-40 parts of Rhizobium fermentation broth, and 10-30 parts of Xanthomonas flavogenes fermentation broth.

3. The lignocellulose-degrading composite microbial agent according to claim 2, characterized in that The components by volume are as follows: 40 parts of Streptomyces tenuiflavus fermentation liquid, 25 parts of Bacillus licheniformis fermentation liquid, 20 parts of Rhizobium fermentation liquid, and 15 parts of Xanthomonas flavogenes fermentation liquid.

4. The lignocellulose-degrading composite microbial agent according to claim 2, characterized in that: The viable bacterial count CFU of the Streptomyces leucoderma fermentation broth is (4.0-5.0)×10 10 The viable bacterial count CFU of the Bacillus licheniformis fermentation broth is (3.0-4.0)×10 10 The viable bacterial count CFU of the rhizobium fermentation broth is (3.0-4.0)×10 10 The viable bacterial count CFU of the Flavescent Cellulomonas fermentation broth is (6.0-7.0)×10 10 .

5. The use of the composite microbial agent for degrading lignocellulose according to any one of claims 1 to 4, characterized in that: The lignocellulose-degrading composite microbial agent is used for degrading agricultural waste rich in lignocellulose.

6. The use according to claim 5, characterized in that: The agricultural waste rich in lignocellulose includes one or more of corn straw, wheat straw, rice straw, rape straw, cottonseed hulls and wood chips.

7. The use according to claim 5, characterized in that The steps include: Step 1: Dry agricultural waste rich in lignocellulose, such as rice straw, corn straw, cottonseed hull, etc. at 60°C to constant weight, then crush and pass through a 40-mesh sieve. Mix the dried agricultural waste with water and add it to a culture dish, sterilize at 121°C for 30 minutes, and obtain an agricultural waste solid culture medium for later use; Step 2: Add the lignocellulose-degrading composite microbial agent to the agricultural waste solid culture medium obtained in step 1, stir evenly, and culture at 25-30° C. for 10-20 days.

8. The use according to claim 7, characterized in that: In step 1, the mass ratio of the dried agricultural waste to water is 1:2.

5.

9. The use according to claim 7, characterized in that: In step 2, the inoculation amount of the lignocellulose-degrading composite microbial agent is 3-5%.

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