10 results about "Ilex asprella" patented technology

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The present application belongs to the technical field of plant cultivation, and particularly relates to a method for improving photosynthetic characteristics and biomass of Ilex asprella and a method for cultivating Ilex asprella. The present application uses any one of three methods of seed soaking, seed irrigation and seed germination for treating Ilex asprella seeds, and inoculates rhizobium, so as to improve the photosynthetic characteristics and biomass of Ilex asprella. The method for cultivating Ilex asprella with high photosynthetic characteristics and biomass comprises the following steps: 1) separating rhizobium; 2) preparing rhizobium liquid with sterile water; 3) treating Ilex asprella seeds by seed germination with the rhizobium liquid to obtain Ilex asprella germinated seeds treated by seed germination with the rhizobium liquid; and 4) culturing the Ilex asprella germinated seeds to obtain Ilex asprella with high photosynthetic characteristics and biomass. The Ilex asprella seedlings treated by seed germination with the rhizobium liquid have higher photosynthetic characteristics and biomass. The present application determines the influence of different inoculation methods on the photosynthetic characteristics and biomass of Ilex asprella seedlings, and provides technical support for cultivating high-quality Ilex asprella.

The invention provides application of a lobular formosan lattuce herb extract with a function of regulating insulin secretion of pancreatic beta cells in preparation of a hypoglycemic drug, and relates to the technical field of natural product extracts. A preparation method of the lobular broadleaf holly leaf extract comprises the following steps: taking high-quality and dry lobular broadleaf holly leaves, crushing, carrying out microwave treatment, and carrying out enzyme treatment; and carrying out water extraction and alcohol extraction, and separating the product. Compared with the prior art, the preparation method has the advantages that the dual advantages of microwave and enzyme treatment are ingeniously fused, the efficiency of the extract is greatly improved, the extract has a remarkable effect in the aspect of improving type 2 diabetes symptoms of model mice, the broadleaf holly leaf component is highly utilized, the loss of precious components is effectively reduced, and the preparation method is suitable for industrial production. The yield is obviously improved. Besides, the preparation method disclosed by the invention is comprehensively optimized in process, is simple and convenient to operate, is stable and reliable, and brings unprecedented development potential and broad prospects for the market.

The application discloses a medicine composition containing Ilex hainanensis, a heat-clearing and inflammation-resolving preparation and a preparation method thereof, and the medicine composition containing Ilex hainanensis comprises the following raw material components in parts by weight: Ilex hainanensis dry extract powder 100 parts; sucralose 0.30-0.40 parts; stevioside 1.00-1.20 parts; citric acid 0.05-0.15 parts. The medicine composition containing Ilex hainanensis and the heat-clearing and inflammation-resolving preparation provided by the application use sucralose, stevioside and citric acid as a composite flavoring agent, the three components are mutually coordinated, the oral compliance of the preparation is successfully improved, the bitterness and astringency of the heat-clearing and inflammation-resolving preparation obtained after the composite flavoring agent is added according to the amount are well improved, there is no bad smell, and the overall taste is good.

The invention discloses a cultivation method for improving comprehensive nutritional value of indigofera pseudotinctoria, which comprises the following steps: in the growth period of indigofera pseudotinctoria, continuously carrying out shading treatment for 3-21 days, and applying nitrogen fertilizer; the whole plant of the indigofera pseudotinctoria is subjected to shading treatment; the growth period of the indigofera pseudotinctoria is a flowering period; the shading intensity of the shading treatment is 50-75%; the nitrogen fertilizer is applied while the shading treatment is started; the fertilizing amount of the nitrogen fertilizer is 1200 kg / hm < 2 >; the shading intensity is 50%; the shading treatment lasts for 14 days. In addition, the invention further provides the indigofera pseudotinctoria cultivated through the cultivation method and application of the cultivation method in improving the comprehensive nutritive value of the indigofera pseudotinctoria. The method has the beneficial effects that the comprehensive nutritional value of the indigofera pseudotinctoria can be effectively improved; the feed additive is excellent in improvement of the crude protein content, can reduce the fiber content (NDF and ADF) to the greatest extent, and remarkably improves the nutritional value, digestibility and palatability of forage grass; and the relatively high coarse ash content means richer mineral nutrition.

This invention discloses a traditional Chinese medicine composition for treating wind-heat type common cold and its preparation method, relating to the field of traditional Chinese medicine technology. The composition is made from the following raw materials in parts by weight: 15-20 parts of *Ilex asprella*, 10-15 parts of mulberry leaf, 4-10 parts of wild chrysanthemum, 4-10 parts of *Viola yedoensis*, 10-15 parts of *Senecio scandens*, and 4-10 parts of dandelion. The preparation method includes: mixing the raw materials, soaking in 6-10 times the amount of water for 15-60 minutes; decocting twice, maintaining the boil for 1.5-2 hours each time, filtering and combining the filtrates; concentrating to a relative density of 1.14-1.18 at 80°C, cooling, adding 95% ethanol to achieve an alcohol content of 65-70%, stirring and standing for 12-24 hours, collecting the supernatant; recovering the ethanol under reduced pressure to obtain an extract and concentrating to a relative density of 1.25-1.30 at 80°C. This invention solves the problems of existing drugs for treating wind-heat colds having a narrow range of indications, insufficient extraction of effective components or incomplete removal of impurities in the preparation process, and the need to improve product quality stability.

The present application relates to a primer combination of a molecular marker for early identification of the gender of bird-attracting plant iron camellia and application thereof, and belongs to the technical field of molecular biology. The primer combination of the molecular marker for early identification of the gender of bird-attracting plant iron camellia comprises an upstream primer and a downstream primer; the nucleotide sequence of the upstream primer is shown as SEQ ID No: 7; and the nucleotide sequence of the downstream primer is shown as SEQ ID No: 8. The present application identifies restriction associated DNA tag fragments (RAD-tags) associated with gender by performing population-level simplified genome deep sequencing on female and male plants of different sources, designs corresponding polymerase chain reaction (PCR) primers, and realizes rapid identification of the gender of iron camellia at an early stage (seedling stage).

The invention relates to the related technical field of cuttage cultivation, in particular to a roughhaired holly tissue culture seedling cultivation device and method based on twig cuttage, the device comprises a cultivation disc with cultivation holes and a base frame, and the top of the cultivation disc is provided with a rotary blocking assembly for transplanting roughhaired holly cutting seedlings in the cultivation holes; a surrounding protection column arranged in the rotary blocking assembly is used for conducting surrounding protection on the roughhaired holly cutting seedlings in the transplanting process, and an overturning soil buckling assembly for huddling soil at the roots of the roughhaired holly cutting seedlings is arranged at the bottom of the surrounding protection column. The soil inserting rods penetrate into soil at the bottoms of the cultivation holes to drive root matrix soil and root systems of the holly root cutting seedlings to rotate synchronously, the binding force of the soil and the hole walls is effectively broken, and the overall rotation loosening of the soil inserting rods and the autorotation wrapping action of the soil lifting rods act on soil and root system complexes in the cultivation holes together.

This invention provides a synergistic antibacterial composition based on small-leaf bitter tea and its preparation method. The composition comprises the following components: 40-60 parts of small-leaf bitter tea extract, 20-30 parts of rosmarinic acid extract, 10-20 parts of baicalin, 5-10 parts of tea polyphenols, and 1-4 parts of pharmaceutical excipients. The preparation method includes the following steps: taking small-leaf bitter tea extract, rosmarinic acid extract, baicalin, tea polyphenols, and pharmaceutical excipients; placing them in a mixer and stirring at 180-240 rpm for 25-35 minutes; passing through an 80-100 mesh sieve to obtain a homogenized composition powder, which is the finished synergistic antibacterial composition based on small-leaf bitter tea. This invention, through the scientific compounding of small-leaf bitter tea extract, rosmarinic acid extract, and baicalin, produces a significant synergistic effect, solving the three major technical problems of traditional small-leaf bitter tea extract: narrow antibacterial spectrum, poor effect against Gram-negative bacteria, insufficient stability, and easy development of drug resistance.

The application relates to a root lifting cultivation method of gold-leaved Ilex asprella, and the specific steps comprise the following steps: step one, shading treatment: the gold-leaved Ilex asprella plants are subjected to shading treatment for at least one month, so that the leaves of the gold-leaved Ilex asprella plants gradually change from yellow to green; step two, root promoting and strengthening cultivation treatment: the gold-leaved Ilex asprella plants with green leaves after the shading treatment in step one are subjected to adventitious root induction treatment and strong root cultivation treatment, so that the root system of the gold-leaved Ilex asprella plants is developed and strong. The application makes the leaves of the gold-leaved Ilex asprella plants change from yellow to green through the shading treatment on the gold-leaved Ilex asprella plants, so that the chlorophyll content accumulated in the leaves is sufficient to supply photosynthetic consumption, so as to reduce the root rot rate and mortality of the gold-leaved Ilex asprella plants in subsequent root lifting cultivation, and batch root lifting cultivation of the gold-leaved Ilex asprella plants is realized.