Primer combination of molecular marker for early identification of gender of bird-attracting plant ilex asprella and application thereof

By performing simplified genome deep sequencing and PCR primer design on Ilex chinensis, the problem of early sex identification of Ilex chinensis was solved, enabling rapid and economical sex identification in the seedling stage and improving landscape and ecological benefits.

CN120060550BActive Publication Date: 2025-11-25GUANGZHOU GREENING CO +3
2 Cites 0 Cited by

Patent Information

Application Number
CN202510316814.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-03-18
Publication Date
2025-11-25
Estimated Expiration
2045-03-18

AI Technical Summary

Technical Problem

Existing technologies cannot effectively identify the sex of Ilex chinensis at an early stage, resulting in a shortage of female plants, which affects the landscape design and ecological benefits. In addition, traditional methods are costly and inefficient.

Method used

By performing population-level simplified genome deep sequencing on male and female plants from different sources, restriction-associated DNA marker fragments associated with sex were identified, and corresponding PCR primers were designed to achieve rapid sex identification of Ilex chinensis at the seedling stage.

Benefits of technology

This method enables rapid and economical identification of the sex of Ilex chinensis during the seedling stage, reducing economic costs and improving landscape design and ecological benefits.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The present application relates to a primer combination of a molecular marker for early identification of the gender of bird-attracting plant iron camellia and application thereof, and belongs to the technical field of molecular biology. The primer combination of the molecular marker for early identification of the gender of bird-attracting plant iron camellia comprises an upstream primer and a downstream primer; the nucleotide sequence of the upstream primer is shown as SEQ ID No: 7; and the nucleotide sequence of the downstream primer is shown as SEQ ID No: 8. The present application identifies restriction associated DNA tag fragments (RAD-tags) associated with gender by performing population-level simplified genome deep sequencing on female and male plants of different sources, designs corresponding polymerase chain reaction (PCR) primers, and realizes rapid identification of the gender of iron camellia at an early stage (seedling stage).
Need to check novelty before this filing date? Find Prior Art