139 results about "Baicalein" patented technology

The invention discloses an application of 4 '-methylamine baicalein in preparation of an anti-photoaging product. Experimental results show that the 4 '-methylamine baicalein not only has very low toxicity to fibroblasts, but also can remarkably improve the multiplication capacity of the fibroblasts irradiated by the UVB and can remarkably inhibit senescence of the fibroblasts irradiated by the UVB, and the effect of the 4'-methylamine baicalein is remarkably superior to that of baicalein; therefore, at least one of 4 '-methylamine baicalein or pharmaceutically acceptable salts, tautomers, stereoisomers and precursor compounds thereof can be used as an active ingredient for preparing anti-photoaging products, and the compound has important significance and application value especially for solving the photoaging problem generated by UVB phototherapy.

The invention belongs to the technical field of nano material synthesis, and particularly relates to baicalein-copper nano microgel as well as a preparation method and application thereof. According to the invention, copper ions and baicalein are coordinated to form nanowires, and the nanowires are coated with hyaluronic acid (HA) to prepare the nano microgel. The method is carried out at normal temperature, is simple in process and high in yield, and does not need toxic solvents; the obtained microgel has high stability (gastric acid resistance), targeted delivery (CD44 receptor mediation) and multiple biological activities (oxidation resistance and inflammation resistance). Animal experiments show that the pharmaceutical composition can significantly relieve ulcerative colitis, the dosage is as low as 5 mg / kg, the pharmaceutical composition is non-toxic, and a new strategy is provided for treatment of inflammatory diseases.

The invention belongs to the technical field of medicines, and discloses application of baicalein in drugs related to the treatment effect of S protein and LPS induced acute lung injury. Specifically, the invention discloses an application of baicalein in preparation of drugs playing a protective role in treating pulmonary edema, inflammatory cell infiltration and cytokine storm caused by acute lung injury induced by S protein combined with LPS. Pharmacological experiments prove that baicalein has an anti-inflammatory effect in treatment of acute lung injury induced by S protein combined with LPS, and can improve pulmonary edema and promote lesion lung tissue repair.

The present invention relates to the technical field of cosmetics, and discloses a whitening and freckle-removing essence and a preparation method thereof. The whitening and freckle-removing essence is formed by mixing additives such as nicotinamide, EDTA, L-lactic acid, citric acid, baicalein inclusion complex, etc. The baicalein inclusion complex has a coated structure. Since the skin surface carries a negative charge and the coating layer of the baicalein inclusion complex contains chitosan, it can be adsorbed on the skin surface through electrostatic action, producing a sustained-release effect, and then targeted release, so as to improve the whitening effect of the essence. In addition, after being encapsulated, baicalein can have good compatibility with other substances in the formula. Moreover, the linolenic fatty acid chain in the structure of the chitosan derivative has excellent antioxidant, anti-inflammatory and other effects, which can reduce the phenomenon of scar formation on the skin due to inflammation.

The invention belongs to the technical field of medicines, and discloses a common goldenrop decoction active ingredient composition and application thereof in prevention and treatment of human respiratory syncytial virus. The invention relates to application of an active ingredient composition (isoliquiritigenin, baicalein, 6-shogaol, schisandrin B, ferulic acid and methyl salicylate) of Xiaochaihu decoction and / or a derivative of the active ingredient or a pharmaceutically acceptable salt thereof in preparation of medicines for preventing, relieving and / or treating RSV (Respiratory Syndrome Virus) infectious diseases. According to the invention, by establishing a mouse RSV infection model, the effect and the action mechanism of the composition in preventing and treating RSV infection are evaluated. Results show that each dose group can inhibit replication of RSV in mouse lung tissues, relieve pulmonary edema, improve pathological changes of the lung, inhibit expression of inflammatory factors and improve humoral immunity, and the effect is equivalent to that of ribavirin. The radix bupleuri granules have better effects on relieving pulmonary edema and inhibiting virus proliferation compared with small radix bupleuri granules. The composition of the effective components of the common goldenrop can provide a new drug treatment scheme for clinical RSV infectious diseases.

The present invention belongs to the technical field of plant extracts, and more specifically, relates to the use of a carob extract in a drug for improving glucose metabolism / polycystic ovary syndrome in women. The present invention discloses a pharmaceutical composition for improving glucose metabolism and treating polycystic ovary syndrome in women, the pharmaceutical composition comprising: carob extract, baicalein, cedrol, a bioactive peptide, a biopolysaccharide, and excipients. The composition of the present invention has the effects of lowering blood sugar and blood lipids, improving insulin function, and also has antioxidant and immunity-enhancing effects. By combining the above substances, a synergistic effect is achieved, further enhancing the effect of improving / treating polycystic ovary syndrome in women associated with abnormal glucose metabolism.

The invention belongs to the technical field of medicines, and provides application of a natural-source compound monomer medicine composition in preparation of medicines for preventing and / or treating pancreatitis. The pharmaceutical composition comprises baicalin, paeoniflorin, geniposide, wogonin, baicalein, albiflorin, tetrahydropalmatine, rhein, wogonin, aloe-emodin, saikoside A, emodin, benzoyl paeoniflorin, chlorogenic acid, costunolide, or a pharmaceutically acceptable salt thereof. Compared with the prior art, the pharmaceutical composition disclosed by the invention has the advantages that the components of the composition are definite and are all natural sources, the safety is good, the pharmaceutical effect has a remarkable synergistic effect, the administration dosage is reduced by about 200 times under the condition that the treatment effect is better than that of a compound traditional Chinese medicine, the administration mode of the compound medicine for treating pancreatitis is innovated, and the pharmaceutical composition is suitable for clinical application. A new scheme is provided for the treatment of the disease.

The invention relates to the technical field of freeze-dried powder, in particular to a freeze-dried powder composition for hair regeneration and a preparation method thereof. The freeze-dried powder composition comprises 5-15 parts of a vascular endothelial growth factor, 3-10 parts of a platelet-derived growth factor, 1-5 parts of copper peptide, 0.5-3 parts of acetyl tetrapeptide-3, 2-8 parts of baicalein, 1-5 parts of asiaticoside, 20-40 parts of a freeze-drying protective agent and 1-3 parts of nano silicon dioxide. The preparation method comprises the following steps: mixing the pretreated vascular endothelial growth factor, platelet-derived growth factor, copper peptide, acetyl tetrapeptide-3, baicalein and asiaticoside with a freeze-drying protective additive and nano silicon dioxide, and freeze-drying. The freeze-dried powder composition can promote hair growth, improve scalp microenvironment and provide lasting support for healthy growth of hair. According to the preparation method, the stability and biological activity of the active ingredients are improved, and the overall performance and safety of the product are improved.

The invention provides a natural Chinese herbal medicine hydrogel composite microneedle and a preparation method and application thereof.The preparation method comprises the steps that S1, natural food-borne nutrients and glycyrrhizic acid are mixed in a solvent, and a glycyrrhizic acid / natural food-borne nutrient solution is obtained; the natural food-borne nutrient is one or more of astaxanthin, baicalein, berberine and curcumin; s2, dropwise adding a metal salt solution into the glycyrrhizic acid / natural food-borne nutrient solution, and performing ultrasonic treatment and stirring to obtain glycyrrhizic acid / food-borne active substance gel; s3, mixing the polyanionic polymer solution with the glycyrrhizic acid / food-borne active substance gel to obtain a mixed solution; and S4, adding the mixed solution into a microneedle mold, carrying out vacuum centrifugation, and drying to obtain the microneedle patch. Good biocompatibility of the natural Chinese herbal medicine extract glycyrrhizic acid is used as a carrier to increase the in-vivo utilization rate of natural food-borne nutrients, so that a better treatment effect is achieved.

The invention relates to an icaritin and baicalein compound pharmaceutical composition and an application thereof in preparation of a medicine for treating skin injury caused by ultraviolet ray B. The effective dose of icaritin is 0.1 mg / g-0. 5mg / g, and the effective dose of baicalein is 1mg / g-5mg / g. According to the invention, the application range of icaritin and baicalein is expanded, and a theoretical basis is provided for applying icaritin and baicalein to clinical treatment of skin injury caused by UVB.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for monitoring a baicalein-betaine eutectic process based on terahertz spectroscopy, and the method comprises the following steps: mixing and ball-milling baicalein and betaine to obtain powder samples, collecting the powder samples with different ball-milling times, drying, mixing with a dispersing agent, tabletting, and drying to obtain the baicalein-betaine eutectic crystal. The method comprises the following steps: measuring spectral data by using terahertz time-domain spectroscopy equipment, selecting a characteristic peak and tracking intensity change to obtain a curve, carrying out nonlinear fitting on the curve, obtaining a molecular vibration mode through a density functional theory, and analyzing the eutectic process by combining the two modes. The terahertz spectrum technology is combined with theoretical calculation, complete analysis of the baicalein-betaine eutectic process from macrodynamics monitoring to microcosmic molecular action mechanism analysis is achieved, and an effective means is provided for eutectic formation research and process optimization.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

This invention belongs to the field of baicalin crystallization technology, and relates to a leaf-shaped baicalin crystal, its preparation method, and its application. The crystal has a leaf-like microstructure with a length ranging from 1 μm to 17 μm; the bulk density of the crystal is 0.1-0.2 g / mL, and the compactness is 0.2-0.4 g / mL. The leaf-shaped baicalin crystals provided by this invention are easy to compress into tablets and have a high surface area / volume ratio, which is beneficial for its adsorption and dissolution in vivo. The preparation process is simple to operate and can directionally prepare leaf-shaped baicalin crystals. The purity of the obtained leaf-shaped baicalin crystals can reach 93% or higher, significantly improving the chemical purity and forming a unique low bulk density physical property.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

The present invention belongs to the field of probe technology, and provides a trifunctional probe based on a photocrosslinking group, a preparation method thereof, and an application thereof. The method comprises the following steps: under a protective atmosphere, compound 1, compound 2, 1-hydroxybenzotriazole, dimethylformamide, carbodiimide, and N,N-diisopropylethylamine are mixed, and a condensation reaction is performed to obtain a trifunctional probe based on a photocrosslinking group. The probe provided by the present invention can be bonded with baicalein, TFAE, and scutellaria baicalensis medicated serum to form a corresponding probe, and compete with its original compound. Under excitation of different ultraviolet wavelengths, the probe can be cross-linked with small molecules and target proteins in traditional Chinese medicine, and accurately reflect the level of target protein labeling of the reactive group in the proteome through a "reporter group", and can also selectively enrich the labeled target protein, and achieve the identification of single compounds, compound combinations, and natural drug extract targets through subsequent experiments.

The invention discloses nasal injection type baicalein hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving carboxymethyl chitosan in water, stirring to form a uniform carboxymethyl chitosan solution, then adding a 4-formylphenylboronic acid / baicalein solution, and then continuously stirring until yellow transparent hydrogel is formed, namely the nasal injection type baicalein hydrogel is obtained. The hydrogel has self-healing ability and proper viscosity, can be adhered to the epithelium of the nasal cavity and is continuously released in the microenvironment of the nasal cavity, so that the brain entering efficiency of the baicalein is remarkably improved; neurotoxic oligomers formed by abnormal aggregation of alpha-syn can be reduced; neuroinflammation can be relieved by regulating and controlling phenotypic polarization of microglial cells M2; oxidative stress can be improved by removing excessive ROS, dopaminergic neuron apoptosis is reduced, and the compound can be used for treating Parkinson's disease and has a good application prospect.

The application aims to provide a preparation method and application of a photocrosslinking zinc-doped baicalin carbon dot-based hydrogel, and belongs to the field of biomedical technology.The baicalin and zinc acetate solution are used as precursors to prepare zinc-doped CDs at 180 DEG C.Meanwhile, a methacrylated gelatin precursor solution is prepared, the carbon dots are added in a certain proportion, and the zinc-doped baicalin carbon dot-based hydrogel is obtained under ultraviolet crosslinking.The carbon dot hydrogel combines the advantages of carbon dots and hydrogels, and has a certain application prospect in wound infection treatment.

The application discloses a kind of Baicalein-loaded mPEG-PLGA nanoparticles based on tumor microenvironment and its preparation method and application.The method is: ultrasonic dispersion preparation Baicalein uniform solution;Then mPEG-PLGA is prepared;Emulsion evaporation method is prepared Baicalein-loaded mPEG-PLGA nanoparticles (PMs-Ba);The nanoparticles can be accumulated in tumor site, improve tumor microenvironment, so as to enhance the sensitivity of breast cancer to chemotherapeutic drugs.The method is simple to operate, provides a new idea for traditional Chinese medicine to be used as adjuvant therapy, and the method has great application potential in early cancer clinical treatment.

This invention provides a magnesium baicalein hydrogel with whitening and anti-skin aging effects and its preparation method. The formulation includes magnesium baicalein, sodium hyaluronate, carbomer, propylene glycol, niacinamide, a pH adjuster, and purified water. The hydrogel uses magnesium baicalein as the main active ingredient, overcoming the traditional deficiency of baicalein's poor water solubility and exhibiting good gel phase stability. The magnesium baicalein in the hydrogel can effectively scavenge free radicals and prevent collagen degradation to combat aging; simultaneously, it inhibits tyrosinase, synergistically achieving a dual whitening effect with niacinamide.

The invention discloses a method for extracting and separating baicalin and baicalein, which comprises the following steps: S1, weighing thymol and menthol, adding the thymol and the menthol into a three-neck flask, and heating and stirring to form uniform and transparent liquid as an extracting agent thymol-menthol eutectic solvent; s2, mixing the radix scutellariae powder with a 50% ethanol solution, heating, condensing and refluxing twice to obtain an extracting solution, centrifuging, taking supernate, and concentrating to obtain a radix scutellariae extracting solution. And S3, putting the scutellaria baicalensis extracting solution and an extracting agent into a high-pressure extraction kettle, carrying out pressurized extraction, transferring to a separating funnel, standing for layering, and respectively collecting. And S4, adding water into the lower-layer water phase for dilution, filtering, heating and stirring, standing at low temperature for crystallization, carrying out suction filtration, washing and drying to obtain purified baicalin. And S5, adding ethyl acetate into the upper-layer extraction phase, oscillating and standing, collecting the lower-layer ethyl acetate, adding anhydrous sodium sulfate, filtering, concentrating, adding absolute methanol, standing at low temperature, crystallizing, carrying out suction filtration, washing and drying to obtain purified baicalein. According to the method for extracting and separating the baicalin and the baicalein, the yield and the purity of the baicalin and the baicalein can be improved.

The invention provides genetically engineered saccharomycetes capable of simultaneously producing baicalein and baicalin as well as a construction method and application of the genetically engineered saccharomycetes. According to the invention, by weakening or deleting a glucose 5-phosphate dehydrogenase gene (ZWF1) and phenylpyruvate decarboxylase genes ARO10 and PDC5 in a saccharomyces cerevisiae genome and enhancing the expression of a pre-benzoic acid dehydratase gene Pha2, the metabolic flow of a shikimic acid pathway is increased, the aldrin pathway is blocked, and the yield of the shikimic acid pathway is increased. Therefore, the yield of L-phenylalanine serving as a starting material for synthesizing baicalein and baicalin is increased; meanwhile, exogenous genes PAL, 4CL, CHS, CHI, FNSII, F6H, CPR and F7GAT are integrated onto the genome by virtue of a gene recombination technology, so that baicalein and baicalin are produced from the beginning by taking glucose and glycerol as a mixed carbon source without adding precursor substances such as exogenous L-phenylalanine or malonyl CoA and the like.

The application belongs to the technical field of biological medicine, and provides application of combined use of tocopherol and mulberroside C in preparation of a drug for treating liver cancer, which is combined use of tocopherol and mulberroside C in preparation of a drug for treating liver cancer. Through in-vitro cell experiments, the growth inhibition effect of tocopherol and mulberroside C on liver cancer cells is evaluated; the results show that tocopherol and mulberroside C can inhibit the growth activity of liver cancer cells to achieve the purpose of treating liver cancer.

This invention discloses a core-shell microneedle with both antibacterial and exudate-absorbing properties, and its preparation method, belonging to the field of microneedle drug delivery systems and acne treatment technology. The microneedle comprises: a core layer containing an exudate-absorbing component for absorbing exudate; and a shell layer covering the core layer, containing a microenvironment-responsive drug delivery unit and antibacterial nanoparticles loaded within the drug delivery unit. The drug delivery unit responds to the microenvironment of the acne lesion by releasing the antibacterial nanoparticles. This invention combines baicalein and surfactant into nanoparticles, loaded into a ROS-responsive shell layer formed by TSPBA and polyvinyl alcohol. Sodium polyglutamate is introduced into the core layer as an exudate-absorbing component. The core-shell microneedle is prepared by a two-step centrifugation method. This invention solves the problems of poor drug permeability, uncontrollable release, and exudate affecting treatment efficacy in traditional acne treatments. It achieves targeted drug release and exudate adsorption synergistic effects on acne lesions, improves drug bioavailability, reduces systemic side effects, and is suitable for the treatment of acne, especially inflammatory and purulent acne.

The present application relates to the technical field of plant extraction, and particularly relates to a process method for jointly preparing baicalein and wogonin, comprising the following steps: (1) crushing the roots of Scutellaria baicalensis Georgi, adding boiling water to extract, and combining the extract; (2) concentrating the extract and adjusting the pH; (3) heating, cooling and adjusting the pH of the concentrated solution to obtain a lysis solution; (4) introducing the lysis solution into a resin column, eluting with purified water and an organic solvent, and concentrating the collected desorption solution; (5) adjusting the pH of the concentrated solution, filtering, drying and crushing the collected first filter cake to obtain a baicalein product; (6) adjusting the pH of the first filtrate and concentrating; (7) cooling, filtering and eluting the concentrated solution, and collecting the second filtrate and second filter cake; (8) adding purified water to the second filter cake to make a pulp, filtering, drying and collecting the third filter cake to obtain a wogonin product; and (9) spray drying the second filtrate to obtain a Scutellaria baicalensis Georgi flavone product. The above process method can simultaneously obtain high-yield baicalein, wogonin and Scutellaria baicalensis Georgi flavone products.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

The present application relates to the technical field of freeze-dried powder, and in particular to a freeze-dried powder composition for hair regeneration and a preparation method thereof. The freeze-dried powder composition comprises 5-15 parts of vascular endothelial growth factor, 3-10 parts of platelet-derived growth factor, 1-5 parts of copper peptide, 0.5-3 parts of acetyl tetrapeptide-3, 2-8 parts of baicalein, 1-5 parts of asiaticoside, 20-40 parts of freeze-drying protectant, and 1-3 parts of nano-silicon dioxide. The preparation method comprises mixing the pretreated vascular endothelial growth factor, platelet-derived growth factor, copper peptide, acetyl tetrapeptide-3, baicalein, and asiaticoside with the freeze-drying protectant and nano-silicon dioxide, and then freeze-drying. The freeze-dried powder composition can promote the growth of hair, improve the microenvironment of the scalp, and provide long-lasting support for the healthy growth of hair. The preparation method improves the stability and biological activity of the active ingredients, and enhances the overall performance and safety of the product.

The present application relates to the technical field of traditional Chinese medicine, and specifically discloses a composition for preventing and treating calf diarrhea, a preparation method and application thereof. The composition for preventing and treating calf diarrhea provided by the present application selects baicalein, forrestimine and traditional Chinese medicine polysaccharide as active ingredients, and the three can produce a synergistic effect, can significantly enhance the inhibitory effect of the composition on bacteria and viruses; at the same time, can also enhance the immunity of the calf by increasing the content of immunoglobulin and cytokine; in addition, can regulate the intestinal flora of the calf, improve the intestinal flora structure, and promote the intestinal health of the calf, thereby effectively preventing and treating the occurrence of calf diarrhea and promoting the health of the calf. The composition provided by the present application effectively prevents and treats calf diarrhea by selecting raw materials with different mechanisms, is not prone to drug resistance, has stable prevention and treatment effect, and has great popularization value.

The invention belongs to the field of feed, and discloses a functional antioxidant which comprises resveratrol, baicalein and taurine. The weight ratio of the resveratrol to the baicalein to the taurine is (100 to 500): (100 to 500): 10000. The functional antioxidant adopts resveratrol, baicalein and taurine as active ingredients, and can comprehensively improve the reproductive performance and lactation performance of sows under the condition of high-temperature stress. In addition, the invention also discloses a related application of the functional antioxidant.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The present invention provides a method for constructing a fingerprint of a traditional Chinese medicine preparation for treating eczema, comprising the following steps: taking gallic acid, gentiopicroside, geniposide, paeoniflorin, ammonium glycyrrhizate, salvianolic acid B, baicalin, baicalein and wogonin reference substances to prepare a mixed reference substance solution; mixing the traditional Chinese medicine preparation for treating eczema with an organic solvent to prepare a test solution; the organic solvent is selected from one or more of methanol aqueous solution, ethanol aqueous solution and anhydrous ethanol; the mixed reference substance solution and the test solution are respectively subjected to high performance liquid chromatography determination; wherein the conditions adopted by the high performance liquid chromatography include: mobile phase A is selected from one or more of methanol and acetonitrile, and mobile phase B is selected from one or more of phosphoric acid aqueous solution, acetic acid aqueous solution and formic acid aqueous solution; and a gradient elution program is adopted.