22 results about "Baicalein" patented technology

This invention belongs to the field of baicalin crystallization technology, and relates to a leaf-shaped baicalin crystal, its preparation method, and its application. The crystal has a leaf-like microstructure with a length ranging from 1 μm to 17 μm; the bulk density of the crystal is 0.1-0.2 g / mL, and the compactness is 0.2-0.4 g / mL. The leaf-shaped baicalin crystals provided by this invention are easy to compress into tablets and have a high surface area / volume ratio, which is beneficial for its adsorption and dissolution in vivo. The preparation process is simple to operate and can directionally prepare leaf-shaped baicalin crystals. The purity of the obtained leaf-shaped baicalin crystals can reach 93% or higher, significantly improving the chemical purity and forming a unique low bulk density physical property.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

This invention provides a magnesium baicalein hydrogel with whitening and anti-skin aging effects and its preparation method. The formulation includes magnesium baicalein, sodium hyaluronate, carbomer, propylene glycol, niacinamide, a pH adjuster, and purified water. The hydrogel uses magnesium baicalein as the main active ingredient, overcoming the traditional deficiency of baicalein's poor water solubility and exhibiting good gel phase stability. The magnesium baicalein in the hydrogel can effectively scavenge free radicals and prevent collagen degradation to combat aging; simultaneously, it inhibits tyrosinase, synergistically achieving a dual whitening effect with niacinamide.

The application belongs to the technical field of biological medicine, and provides application of combined use of tocopherol and mulberroside C in preparation of a drug for treating liver cancer, which is combined use of tocopherol and mulberroside C in preparation of a drug for treating liver cancer. Through in-vitro cell experiments, the growth inhibition effect of tocopherol and mulberroside C on liver cancer cells is evaluated; the results show that tocopherol and mulberroside C can inhibit the growth activity of liver cancer cells to achieve the purpose of treating liver cancer.

This invention discloses a core-shell microneedle with both antibacterial and exudate-absorbing properties, and its preparation method, belonging to the field of microneedle drug delivery systems and acne treatment technology. The microneedle comprises: a core layer containing an exudate-absorbing component for absorbing exudate; and a shell layer covering the core layer, containing a microenvironment-responsive drug delivery unit and antibacterial nanoparticles loaded within the drug delivery unit. The drug delivery unit responds to the microenvironment of the acne lesion by releasing the antibacterial nanoparticles. This invention combines baicalein and surfactant into nanoparticles, loaded into a ROS-responsive shell layer formed by TSPBA and polyvinyl alcohol. Sodium polyglutamate is introduced into the core layer as an exudate-absorbing component. The core-shell microneedle is prepared by a two-step centrifugation method. This invention solves the problems of poor drug permeability, uncontrollable release, and exudate affecting treatment efficacy in traditional acne treatments. It achieves targeted drug release and exudate adsorption synergistic effects on acne lesions, improves drug bioavailability, reduces systemic side effects, and is suitable for the treatment of acne, especially inflammatory and purulent acne.

This invention discloses a differentiated fertilization method for Scutellaria baicalensis, belonging to the field of traditional Chinese medicinal herb cultivation technology. This method selects a corresponding ratio from multiple predetermined nitrogen, phosphorus, and potassium fertilizer application ratios based on the accumulation requirements of the target medicinal components, thereby achieving targeted regulation of different medicinal components. The multiple predetermined nitrogen-phosphorus-potassium ratios include a first ratio that increases baicalin, a second ratio that increases baicalein, a third ratio that increases wogonin, and a fourth ratio that increases wogonein. This invention allows for flexible selection of ratios according to production targets, achieving precise regulation of different medicinal components while simultaneously increasing Scutellaria baicalensis yield, and has good prospects for widespread application.

The invention provides a method for detecting the contents of multiple index components of a coughing oral liquid, which comprises the following steps of: determining the contents of nine key pharmacodynamic index components, namely ephedrine hydrochloride, pseudoephedrine hydrochloride, apiose liquiritin, isoliquiritin, baicalin, huperzin A glycoside, wogonin, baicalein and wogonin; the detection method has the advantages of simplicity and convenience in operation, high stability, high pertinence and high practicability, can be used as a detection and evaluation method for production and internal quality components of the coughing oral liquid, and has a good market application prospect.

This invention discloses a three-layered functional hydrogel material for osteochondral repair that promotes chondrogenesis through traditional Chinese medicine and physical stimulation, and its preparation method. Specifically, the method includes: 1) preparing a PCL electrospun membrane using electrospinning technology, and forming an HA coating on its surface using a hydrothermal method with biomimetic mineralization; 2) constructing the HA coating on the surface of black phosphorus sheets and preparing cryptotanshinone nanopowder using in-situ mineralization and spraying methods, and mixing it with BMSCs in a porous GelMA solution; 3) preparing MCSN nanoparticles loaded with baicalein using a hydrothermal template method and ultrasonic impregnation process; 4) dispersing barium titanate nanowires, drug-loaded MCSN, and BMSCs in a SilMA / HAMA solution; 5) pouring the GelMA mixture into a mold and pre-curing it, placing a plasma-treated mineralized electrospun membrane on top, then pouring the SilMA / HAMA mixture and completely curing it to obtain the desired hydrogel. This composite material has a three-layer structure, matching the needs of osteochondral repair.

The application relates to the technical field of stem cell engineering and regenerative medicine, and specifically discloses a stem cell treatment method for treating type 2 diabetes. The method comprises the following steps: S1, extracting placenta-derived mesenchymal stem cells; S2, performing multi-gene modification on the mesenchymal stem cells to obtain gene-modified mesenchymal stem cells; S3, pretreating the gene-modified mesenchymal stem cells with a culture medium; S4, extracting microvesicles from the mesenchymal stem cells; S5, mixing the microvesicles, baicalin, L-arginine and vitamin D in a buffer to form a composite preparation; S6, introducing the composite preparation into a patient's body through abdominal cavity intervention; and S7, performing synergistic treatment by combining oral administration of an SGLT2 inhibitor after infusion, and regularly monitoring islet function indexes. The stem cell treatment method can be used for treating type 2 diabetes, and has the advantages of being targeted to improve the islet microenvironment, promoting the recovery of beta cell function and being durable in action.

The application discloses application of total flavones of radix scutellariae combined with tetracycline antibiotics in preparation of products against staphylococcus aureus, and belongs to the technical field of medicines. The application provides an antibacterial composition containing total flavones of radix scutellariae and tetracycline antibiotics, aiming at the problems of high drug resistance rate and great toxic side effects of tetracycline antibiotics in the treatment of staphylococcus aureus and methicillin-resistant staphylococcus aureus infection. The total flavones of radix scutellariae contain more than 89% of flavones, and contain various active ingredients such as baicalin and baicalein. The tetracycline antibiotics are selected from doxycycline or minocycline. In vitro experiments prove that the combination of the two has a significant synergistic antibacterial effect on staphylococcus aureus and drug-resistant staphylococcus aureus, and the dosage of doxycycline or minocycline can be reduced by 75%, thereby effectively reducing the toxic side effects.

The application discloses application of baicalein in prevention and control of soil-borne bacterial wilt. The application discloses application of baicalein in preparation of a preparation for preventing and controlling soil-borne bacterial wilt. The application discloses a method for preventing and controlling soil-borne bacterial wilt, which comprises the following steps: after transplanting seedlings of plants for 7-10 days, baicalein solution is applied by means of root irrigation. In the application, baicalein is applied by means of root irrigation, which can promote secretion of bacteriostatic substances of beneficial bacterial groups in the rhizosphere of solanaceous crops such as tomatoes, inhibit bacterial wilt, reduce the incidence of tomato bacterial wilt, and prevent and control soil-borne bacterial wilt.

The present application relates to the technical field of cell engineering, and particularly relates to a culture medium for producing biological antibodies and a fermentation method thereof.The culture medium comprises a basic culture medium and a fermentation culture medium; the basic culture medium is based on a commercial culture medium and comprises the following components: fumaric acid, ferric ammonium citrate, astragaloside and baicalein; the fermentation culture medium is based on a commercial culture medium and comprises the following components: L-citrulline, L-ornithine, L-theanine, L-homoarginine, L-hydroxyproline, L-allo-threonine, mannose, isorhamnetin and ginkgolin.The culture medium combination can effectively improve the expression level of target antibodies, supports cells to maintain long-term activity in a high-density state; meanwhile, the charge variant distribution of the obtained antibodies is more uniform, and the product purity and consistency are significantly improved.

This invention utilizes the recombinant porcine deltacoronavirus protein PDCoV-S constructed in an insect-baculovirus expression system, and uses it as an immunogen to prepare egg yolk antibodies. The egg yolk antibodies are further processed into oral formulations. Through optimization of emulsifiers and synergists, microcapsule formulations with high encapsulation efficiency, high gastric acid throughput, and good intestinal release performance are obtained. Furthermore, the addition of synergists enhances the therapeutic efficacy of the formulation. When the mass ratio of baicalin, Polygonatum odoratum polysaccharide, and nano-selenium is 2:1:2, the encapsulation efficiency of the microcapsules can be effectively improved, thus enhancing the drug's effect. This microcapsule formulation can be used as an active ingredient in the production of drugs or feed additives for the treatment or prevention of PDCoV infection.

This invention proposes a compound plant composition for inhibiting intestinal inflammatory response in pigs, its preparation method, and its application, relating to the field of veterinary technology. The compound plant composition contains the following active ingredients in parts by weight: 50-70 parts carvacrol, 10-20 parts thymol, 30-50 parts cinnamaldehyde, 5-10 parts baicalein, 5-10 parts apigenin, and 3-8 parts chlorogenic acid. The preparation method involves mixing the active ingredients in proportion, adding water and Tween-80, and stirring until homogeneous. This invention can significantly reduce the levels of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α in the serum and small intestinal tissue of pigs infected with enterotoxigenic Escherichia coli, and increase the level of the anti-inflammatory cytokine IL-10. It also exerts its anti-inflammatory effect by inhibiting the activation of the TLR4-MyD88-NF-κB signaling pathway. This invention uses a compound of natural plant extracts, eliminating the risk of antibiotic resistance and veterinary drug residues, and is simple to prepare.

This invention provides a method for extracting and preparing cumin essential oil and its application in pet insect repellent spray products, belonging to the field of daily chemical technology. The cumin essential oil of this invention is extracted using an optimized eutectic solvent (DES) extraction method. The extracted cumin essential oil is then combined with a complex plant extract (including sesame, artemisia, and baicalein extract), daily fragrance, alcohol, and water to form a pet insect repellent spray. This invention uses a combination of plant essential oils and complex extracts as the main active insect repellent ingredients, possessing advantages such as being natural, green, safe, without side effects, and pollution-free, laying the foundation for the development of green and environmentally friendly plant-based mosquito repellent products.

This invention discloses a nanocomposite, its preparation method, and its application. The preparation method involves dissolving cerium nitrate and polyethylene glycol in water, and baicalein in anhydrous ethanol. The mixtures are stirred and baicalein solution is added dropwise. After centrifugation, washing, and drying, the target nanocomposite is obtained. This invention offers mild conditions and good reproducibility, allowing for the one-step production of a nanocomposite with uniform particle size. This nanocomposite synergistically combines the enzyme-like activity of cerium ions with the anti-inflammatory and antioxidant effects of baicalein, effectively clearing excess ROS after spinal cord injury, significantly inhibiting inflammatory responses, protecting neurons, and promoting axonal regeneration and functional recovery. This provides a novel, highly efficient, and safe nanomedicine for the treatment of spinal cord injury.

This invention provides a synergistic antibacterial composition based on small-leaf bitter tea and its preparation method. The composition comprises the following components: 40-60 parts of small-leaf bitter tea extract, 20-30 parts of rosmarinic acid extract, 10-20 parts of baicalin, 5-10 parts of tea polyphenols, and 1-4 parts of pharmaceutical excipients. The preparation method includes the following steps: taking small-leaf bitter tea extract, rosmarinic acid extract, baicalin, tea polyphenols, and pharmaceutical excipients; placing them in a mixer and stirring at 180-240 rpm for 25-35 minutes; passing through an 80-100 mesh sieve to obtain a homogenized composition powder, which is the finished synergistic antibacterial composition based on small-leaf bitter tea. This invention, through the scientific compounding of small-leaf bitter tea extract, rosmarinic acid extract, and baicalin, produces a significant synergistic effect, solving the three major technical problems of traditional small-leaf bitter tea extract: narrow antibacterial spectrum, poor effect against Gram-negative bacteria, insufficient stability, and easy development of drug resistance.

The application belongs to the technical field of nanomaterial synthesis and neurology, and particularly relates to a baicalein carbon dot as well as a preparation method and application thereof. The baicalein carbon dot is directly synthesized by hydrothermal reaction with baicalein as a main carbon source and a nitrogen-containing organic ligand (biotin, glutathione or dopamine hydrochloride). The baicalein carbon dot can efficiently penetrate the blood-brain barrier, scavenge reactive oxygen species and inhibit the aggregation of A beta protein. The baicalein carbon dot can be coupled with a transferrin-targeting derivative constructed by an EDC-NHS method. The baicalein carbon dot and the derivative prepared by the application have a wide application prospect in the preparation of drugs for preventing and / or treating Alzheimer's disease.

This invention discloses a microcapsule with time-released baicalein and asiatic acid nanoparticles, its preparation method, and its application. The preparation method of the microcapsule includes: firstly, synthesizing baicalein nanoparticles and asiatic acid liposomes separately; then, using microfluidic electrospray technology, preparing core-shell structured microdroplets with a sodium alginate solution loaded with baicalein nanoparticles as the outer phase and a sodium alginate solution loaded with asiatic acid liposomes as the inner phase; finally, solidifying with calcium chloride solution to obtain core-shell microcapsules. The microcapsule of this invention utilizes its unique core-shell structure to preferentially release baicalein nanoparticles in the shell layer in a physiological environment to achieve early antibacterial effects, followed by the release of asiatic acid liposomes in the core layer to promote later cell migration and tissue repair, achieving time-sequential drug release that matches the wound healing process. This microcapsule significantly improves the bioavailability and delivery controllability of poorly soluble traditional Chinese medicine active ingredients, and has broad application prospects in the field of wound repair.

This invention discloses a β-glucuronidase and its enzymatic hydrolysis method for preparing baicalin and glucuronolactone, belonging to the field of genetic engineering technology. The amino acid sequence of this β-glucuronidase is obtained by mutation of the amino acid sequence shown in SEQ ID NO.1, specifically a mutation at amino acid position 296 and / or amino acid position 329 of the amino acid sequence shown in SEQ ID NO.1. The mutated β-glucuronidase mutant obtained by this invention exhibits higher enzyme activity and stronger hydrolytic activity towards the substrate baicalin, thereby effectively improving reaction efficiency and substrate conversion rate in the hydrolysis of baicalin to produce baicalin and glucuronic acid.

The application provides a emodin-8-O-beta-D-glucoside / baicalein hydrogel and a preparation method and application thereof, and the preparation method comprises the following steps: dissolving baicalein in a PBS solution with a pH of 7-8 to obtain a baicalein solution; mixing emodin-8-O-beta-D-glucoside with the baicalein solution to obtain a mixed solution; and performing ultrasonic dispersion on the mixed solution at an ultrasonic power of 70 W-120 W and at 50 DEG C-70 DEG C to obtain the emodin-8-O-beta-D-glucoside / baicalein hydrogel. The emodin-8-O-beta-D-glucoside / baicalein hydrogel prepared by the method has the advantages that emodin-8-O-beta-D-glucoside and baicalein are directly self-assembled into a hydrogel without metal ions, organic polymers and organic solvents, and the hydrogel has obvious curative effect in the preparation of drugs for treating diseases such as post-brain injury neural function defect, blood-brain barrier injury and excessive activation of neutrophil extracellular traps.

This invention relates to the use of baicalin in the preparation of drugs for treating and / or alleviating preeclampsia. The drugs are used to inhibit ferroptosis in placental trophoblasts and / or placental tissue, manifested by increasing the expression level of at least one of GPX4, SLC7A11, and FTH1, increasing GSH content and / or GPX activity, and decreasing Fe... 2+ It can reduce MDA, lipid ROS and / or mitochondrial ROS levels, and improve preeclampsia-related hypertension, proteinuria, placental oxidative stress and adverse pregnancy outcomes. This application can be used to prepare preeclampsia treatment and / or alleviation drugs with baicalin monomer as the active ingredient.