7 results about "Glucuronolactone" patented technology

This invention belongs to the field of animal feed technology and discloses the application of glucuronolactone in the preparation of feed that alleviates spleen damage caused by the combined effects of PRRSV and DON. This invention discovers that glucuronolactone can effectively alleviate spleen damage in piglets caused by co-exposure to PRRSV and DON. It exerts its protective effect by activating the Nrf2 antioxidant signaling pathway, improving spleen tissue structure, alleviating oxidative stress, inhibiting inflammatory responses, and inhibiting apoptosis. This invention also provides a feed containing the aforementioned effective amount of glucuronolactone, with the basic feed tailored to the nutritional needs of piglets. This invention fills the technological gap in effective intervention methods for spleen damage under PRRSV and DON co-exposure. The glucuronolactone addition process is simple, the effect is significant, and it is suitable for large-scale feed production. It can effectively solve the problem of immunosuppression in pig herds caused by the combined harm of PRRSV and DON in the pig industry, and has important practical value.

The application discloses a stem cell exosome-containing composition and application of the stem cell exosome-containing composition in preparation of a medicine with a liver damage treatment effect, and the stem cell exosome-containing composition comprises, by weight, 0.1-0.5 parts of umbilical cord mesenchymal stem cell exosome, 3-6 parts of pueraria extract, 0.2-1 part of honey, 0.06-0.32 parts of compound vitamin, 0.3-0.7 parts of olive oil, 0.08-0.2 parts of starch, 0.03-0.15 parts of glutamic acid, 0.03-0.15 parts of cysteine, 0.03-0.15 parts of glycine, 0.05-0.35 parts of glucuronolactone, 0.05-0.18 parts of sodium carboxymethyl cellulose and 22-37 parts of deionized water. The stem cell exosome can adjust cell apoptosis, growth, multiplication and differentiation pathways through the transcriptome and proteome of a receptor cell, thereby delaying the occurrence and development of liver diseases; the pueraria extract is rich in various flavones and isoflavones, can promote liver cell regeneration, and provides required nutrients for the liver in cooperation with other components, and effectively treats liver damage.

The invention discloses a method for detecting related substances of calcium gluconate. The detection method is a high performance liquid chromatography-electric fog type detection method. Chromatographic conditions are as follows: a chromatographic column takes cation exchange resin as a filler, the evaporation temperature is 50-60 DEG C, the collection frequency is 8-12 Hz, the filtration constant is 4.5-5.5 s, the power function value is 1.8-2.2, the column temperature is 45-55 DEG C, the mobile phase is 0.09-0.11% trifluoroacetic acid solution, and isocratic elution is performed. The method is used; d-glucuronic acid, isomaltohexanoic acid, maltose acid, fructose, D-glucuronic acid lactone, glucose, D-gluconic acid-gamma-lactone, glucaric dialdehyde, D-gluconic acid-delta-lactone, oxalic acid, maltotriose, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2- According to the method, impurities such as 3, 5-hydroxymethyl-2-furaldehyde and the like are well separated from calcium gluconate, and the impurities in the method are relatively good in sensitivity, relatively small in baseline interference, relatively good in specificity and the like, so that the defects in the prior art can be effectively overcome, and related substances in the calcium gluconate can be effectively controlled; the safety, the effectiveness and the quality controllability of various calcium supplements prepared by taking the calcium gluconate as the raw material medicine are ensured.

The application discloses a multifunctional hydrogel, a preparation method and application, and the multifunctional hydrogel is a sodium alginate-poloxamer 407 hydrogel loaded with glucuronolactone and tannic acid. 2+ The multifunctional hydrogel realizes multifunctional synergy through the joint introduction of glucuronolactone and tannic acid, retains the temperature-sensitive injectable property, and has a more optimal comprehensive effect in microenvironment regulation, oxidative stress relief, inflammation reaction inhibition and cell function improvement. Animal experiment data show that, compared with ordinary sodium alginate-poloxamer 407 hydrogel injection treatment and traditional sodium hyaluronate injection treatment, the injection treatment based on the multifunctional hydrogel can more effectively inhibit cartilage tissue degeneration, relieve joint swelling, improve pain sensitivity and motor dysfunction. Moreover, the multifunctional hydrogel has a simple preparation method, high biological safety and an excellent clinical application prospect.

This invention relates to a crosslinking agent for improving the thermal stability of amino substrates and its application. The crosslinking agent for improving the thermal stability of amino substrates is one or more of glucuronic acid, galacturonic acid, glucuronide, methyl glucuronide, and methyl galacturonide. It improves substrate performance by reacting with the amino substrate through Maillard reactions or amidation reactions via its contained active aldehyde, carboxyl, or ester groups. This crosslinking agent exhibits significant crosslinking effect, good biocompatibility, and high permeability.

This invention discloses the application of a feed additive that alters the fatty acid composition of egg yolk lipids in feed. The invention regulates the content of polyunsaturated and monounsaturated fatty acids in egg yolk lipids by adding glucuronolactone to poultry feed. The polyunsaturated fatty acids include n-3 series polyunsaturated fatty acids. Adding glucuronolactone to the feed alters the fatty acid composition of egg yolk lipids. By regulating the content of polyunsaturated and monounsaturated fatty acids in egg yolk lipids, especially the content of n-3 series polyunsaturated fatty acids, it not only increases the functional nutritional value of the egg yolk and improves the quality of the egg, but also enhances the flavor of the egg yolk.

This invention discloses a β-glucuronidase and its enzymatic hydrolysis method for preparing baicalin and glucuronolactone, belonging to the field of genetic engineering technology. The amino acid sequence of this β-glucuronidase is obtained by mutation of the amino acid sequence shown in SEQ ID NO.1, specifically a mutation at amino acid position 296 and / or amino acid position 329 of the amino acid sequence shown in SEQ ID NO.1. The mutated β-glucuronidase mutant obtained by this invention exhibits higher enzyme activity and stronger hydrolytic activity towards the substrate baicalin, thereby effectively improving reaction efficiency and substrate conversion rate in the hydrolysis of baicalin to produce baicalin and glucuronic acid.