13 results about "Glucuronolactone" patented technology

The application discloses a glucuronolactone and a preparation method thereof, and belongs to the technical field of fine chemical product preparation. The method takes potassium phytate extracted from corn soaking liquid or rice bran acid soaking liquid as raw material, obtains inositol and potassium dihydrogen phosphate after hydrolysis, separates the hydrolysis liquid through chromatography, obtains potassium dihydrogen phosphate by concentrating and crystallizing the salt phase, and the material phase is inositol solution. Enzymatic conversion reaction is carried out by using fermentation to obtain enzyme liquid, glucuronic acid liquid is obtained, glucuronolactone is obtained by concentrating and esterifying, and then the glucuronolactone is obtained by concentrating, refining and crystallizing. In the method, the yield of potassium phytate to inositol is greater than 95%, the molar conversion rate of inositol enzymatic conversion to glucuronic acid is greater than 95%, and the total yield is greater than 89%, which is much higher than the traditional yield, and the content of the obtained glucuronolactone is greater than 99.2%. The glucuronolactone is prepared by enzymatic conversion of the intermediate inositol, the by-product is high-value potassium dihydrogen phosphate, the whole has good economic benefits, and industrial production can be realized.

The present invention discloses a preparation method of glucurone, which relates to the technical field of esterification reactions. The method comprises the following steps: adding inositol oxidase to an inositol feed solution for conversion to obtain a conversion solution, and successively subjecting the conversion solution to filtration, ion exchange, decolorization, thermal concentration, and lactonization crystallization steps to obtain crude glucurone. The preparation method of the present invention uses inositol as a raw material, and after converting inositol into glucuronic acid solution under the action of an enzyme, adopts filtration, ion exchange, decolorization, thermal concentration, and lactonization crystallization steps to prepare crude glucurone. By adopting the technical solution of the present invention to prepare glucurone, the problems of low extraction rate of crude products, severe operating pressure, strict equipment requirements, and environmental pollution existing in the nitric acid oxidation method using starch as a raw material are solved. At the same time, the present invention uses inositol as a raw material, which is a new process different from the process using starch as a raw material, and has the advantages of low production cost, short cycle, and industrialization feasibility.

This invention belongs to the field of animal feed technology and discloses the application of glucuronolactone in the preparation of feed that alleviates spleen damage caused by the combined effects of PRRSV and DON. This invention discovers that glucuronolactone can effectively alleviate spleen damage in piglets caused by co-exposure to PRRSV and DON. It exerts its protective effect by activating the Nrf2 antioxidant signaling pathway, improving spleen tissue structure, alleviating oxidative stress, inhibiting inflammatory responses, and inhibiting apoptosis. This invention also provides a feed containing the aforementioned effective amount of glucuronolactone, with the basic feed tailored to the nutritional needs of piglets. This invention fills the technological gap in effective intervention methods for spleen damage under PRRSV and DON co-exposure. The glucuronolactone addition process is simple, the effect is significant, and it is suitable for large-scale feed production. It can effectively solve the problem of immunosuppression in pig herds caused by the combined harm of PRRSV and DON in the pig industry, and has important practical value.

The application discloses a stem cell exosome-containing composition and application of the stem cell exosome-containing composition in preparation of a medicine with a liver damage treatment effect, and the stem cell exosome-containing composition comprises, by weight, 0.1-0.5 parts of umbilical cord mesenchymal stem cell exosome, 3-6 parts of pueraria extract, 0.2-1 part of honey, 0.06-0.32 parts of compound vitamin, 0.3-0.7 parts of olive oil, 0.08-0.2 parts of starch, 0.03-0.15 parts of glutamic acid, 0.03-0.15 parts of cysteine, 0.03-0.15 parts of glycine, 0.05-0.35 parts of glucuronolactone, 0.05-0.18 parts of sodium carboxymethyl cellulose and 22-37 parts of deionized water. The stem cell exosome can adjust cell apoptosis, growth, multiplication and differentiation pathways through the transcriptome and proteome of a receptor cell, thereby delaying the occurrence and development of liver diseases; the pueraria extract is rich in various flavones and isoflavones, can promote liver cell regeneration, and provides required nutrients for the liver in cooperation with other components, and effectively treats liver damage.

The present invention relates to a universal acid-base water-based zinc plating additive and its application. The universal acid-base water-based zinc plating additive comprises 5wt.% to 20wt.% of polyepichlorohydrin-nitrogen-containing heterocyclic-chain amine, 5wt.% to 10wt.% of trigonelline, 1wt.% to 5wt.% of compound A, 2wt.% to 8wt.% of sodium benzoate, 5wt.% to 10wt.% of tetrahydroxypropylethylenediamine, 0.5wt.% to 5wt.% of glucuronolactone, and the balance is water; wherein compound A is a reactant of nicotinic acid and propane sultone. The universal acid-base water-based zinc plating additive can be used in three production lines: alkaline zinc plating, sulfate zinc plating, and potassium chloride zinc plating. The organic pollutants in the wastewater of the three production lines can be made the same, facilitating unified treatment.

The present invention discloses a modified phenolic resin-based hard carbon material and its preparation method and application, belonging to the technical field of phenolic resin-based hard carbon materials. The present invention creatively uses aldehyde-containing, oxygen-rich glucuronolactone to replace formaldehyde in traditional methods, and uses 1,2,4-pyrogallol containing three phenolic hydroxyl groups, two of which are in the ortho position, to replace phenol in traditional methods. The provided method is easy to prepare thermosetting phenolic resin, and has the advantages of low cost, non-toxicity and pollution-free. The prepared modified phenolic resin-based hard carbon material has a suitable specific surface area, abundant micropores and a larger graphitized interlayer spacing. The specific surface area of the modified phenolic resin-based hard carbon material is 451.51m 2 / g, and the micropore volume is 0.217cm 3 / g; the prepared modified phenolic resin-based hard carbon material is used as the negative electrode material of sodium ion battery, with an initial coulombic efficiency of 86.44%, a platform capacity of 232.8mAh / g at a current density of 0.05A / g, and a capacity retention rate of 83.71% after stable circulation for 1000 cycles with a coulombic efficiency of 99.97% at a current density of 1A / g.

The invention discloses a method for detecting related substances of calcium gluconate. The detection method is a high performance liquid chromatography-electric fog type detection method. Chromatographic conditions are as follows: a chromatographic column takes cation exchange resin as a filler, the evaporation temperature is 50-60 DEG C, the collection frequency is 8-12 Hz, the filtration constant is 4.5-5.5 s, the power function value is 1.8-2.2, the column temperature is 45-55 DEG C, the mobile phase is 0.09-0.11% trifluoroacetic acid solution, and isocratic elution is performed. The method is used; d-glucuronic acid, isomaltohexanoic acid, maltose acid, fructose, D-glucuronic acid lactone, glucose, D-gluconic acid-gamma-lactone, glucaric dialdehyde, D-gluconic acid-delta-lactone, oxalic acid, maltotriose, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2- According to the method, impurities such as 3, 5-hydroxymethyl-2-furaldehyde and the like are well separated from calcium gluconate, and the impurities in the method are relatively good in sensitivity, relatively small in baseline interference, relatively good in specificity and the like, so that the defects in the prior art can be effectively overcome, and related substances in the calcium gluconate can be effectively controlled; the safety, the effectiveness and the quality controllability of various calcium supplements prepared by taking the calcium gluconate as the raw material medicine are ensured.

The present invention discloses a method for producing D-glucuronic acid by biotransformation and a method for producing glucuronolactone using D-glucuronic acid, and relates to the technical field of biological production. The method for producing D-glucuronic acid by biotransformation described above includes the steps of culturing a recombinant engineering bacterium to obtain a seed solution, inoculating the seed solution into a fermenter medium for fermentation culture, obtaining a fermentation broth through feed control and induction control, centrifuging or membrane filtering the fermentation broth to obtain wet bacterial cells, and adding the wet bacterial cells to a reaction solution for conversion to obtain D-glucuronic acid. Further, the method for producing glucuronolactone using the above-mentioned D-glucuronic acid includes further adding concentrated phosphoric acid to a D-glucuronic acid solution, performing an esterification reaction at a reaction temperature of 40°C to 80°C while stirring, and crystallizing to obtain a crude product of glucuronolactone. When the fermentation process of the present invention is adopted to produce D-glucuronic acid, the conversion rate of inositol can reach 95% or more, the content of the obtained D-glucuronic acid is greater than 76 g / L, and inositol oxidase can be repeatedly used. By adopting the production process of glucuronolactone of the present invention to produce glucuronolactone, the total time from the reaction to the completion of crystallization is less than 6 h, significantly reducing the reaction crystallization time, shortening the production cycle, and improving the production efficiency.

The application discloses a multifunctional hydrogel, a preparation method and application, and the multifunctional hydrogel is a sodium alginate-poloxamer 407 hydrogel loaded with glucuronolactone and tannic acid. 2+ The multifunctional hydrogel realizes multifunctional synergy through the joint introduction of glucuronolactone and tannic acid, retains the temperature-sensitive injectable property, and has a more optimal comprehensive effect in microenvironment regulation, oxidative stress relief, inflammation reaction inhibition and cell function improvement. Animal experiment data show that, compared with ordinary sodium alginate-poloxamer 407 hydrogel injection treatment and traditional sodium hyaluronate injection treatment, the injection treatment based on the multifunctional hydrogel can more effectively inhibit cartilage tissue degeneration, relieve joint swelling, improve pain sensitivity and motor dysfunction. Moreover, the multifunctional hydrogel has a simple preparation method, high biological safety and an excellent clinical application prospect.

This invention relates to a crosslinking agent for improving the thermal stability of amino substrates and its application. The crosslinking agent for improving the thermal stability of amino substrates is one or more of glucuronic acid, galacturonic acid, glucuronide, methyl glucuronide, and methyl galacturonide. It improves substrate performance by reacting with the amino substrate through Maillard reactions or amidation reactions via its contained active aldehyde, carboxyl, or ester groups. This crosslinking agent exhibits significant crosslinking effect, good biocompatibility, and high permeability.

This invention discloses the application of a feed additive that alters the fatty acid composition of egg yolk lipids in feed. The invention regulates the content of polyunsaturated and monounsaturated fatty acids in egg yolk lipids by adding glucuronolactone to poultry feed. The polyunsaturated fatty acids include n-3 series polyunsaturated fatty acids. Adding glucuronolactone to the feed alters the fatty acid composition of egg yolk lipids. By regulating the content of polyunsaturated and monounsaturated fatty acids in egg yolk lipids, especially the content of n-3 series polyunsaturated fatty acids, it not only increases the functional nutritional value of the egg yolk and improves the quality of the egg, but also enhances the flavor of the egg yolk.

This invention discloses a β-glucuronidase and its enzymatic hydrolysis method for preparing baicalin and glucuronolactone, belonging to the field of genetic engineering technology. The amino acid sequence of this β-glucuronidase is obtained by mutation of the amino acid sequence shown in SEQ ID NO.1, specifically a mutation at amino acid position 296 and / or amino acid position 329 of the amino acid sequence shown in SEQ ID NO.1. The mutated β-glucuronidase mutant obtained by this invention exhibits higher enzyme activity and stronger hydrolytic activity towards the substrate baicalin, thereby effectively improving reaction efficiency and substrate conversion rate in the hydrolysis of baicalin to produce baicalin and glucuronic acid.

The invention discloses application of glucurolactone in preparation of feed for preventing and treating weaning stress of piglets, and belongs to the technical field of animal feed. The invention discloses application of glucurolactone in preparation of a feed for preventing and treating weaning stress of piglets. The glucurolactone is used for preparing the feed, and the glucurolactone in the feed can effectively improve the oxidation resistance and the immunity of the weaned piglets, improve the intestinal health of the piglets and improve the growth performance and the health level of the weaned piglets.