63 results about "Trigonelline" patented technology

The present invention relates to a pharmaceutical composition having dopaminergic activity and other related pharmaceutical activities comprising trigonelline or its derivative(s) and 4-hydroxyisoleucine or its derivative(s), optionally along with excipients(s); a process of preparing a pharmaceutical composition comprising trigonelline or its derivative(s) and 4-hydroxyisoleucine or its derivative(s), optionally along with excipients(s), wherein the process comprising steps of: (a) extracting a clear solution containing trigonelline and 4-hydroxyisoleucine from plant source; and (b) optionally precipitating derivative(s) of trigonelline and 4-hydroxyisoleucine from the clear solution and obtaining said composition; and an in-vitro method to increase levels of dopamine or to inhibit prolactin by allowing composition comprising trigonelline or its derivative(s) and 4-hydroxyisoleucine or its derivative(s) to bind to cell receptors.

The invention relates to pumpkin vermicelli, mainly comprising pumpkin meal, sweet potato meal and cornmeal in a ratio of 50-51:30-38:11-20. The ratio of the mixture of the pumpkin meal, sweet potato meal and cornmeal to water is 100:78-86. The production process comprises blending, stirring, forming, freezing, airing, traying and shaping, secondary airing, packaging and finished product preparing. The pumpkin vermicelli has the following benefits: the selection of the raw materials is scientific; and the pumpkin vermicelli contains fruit acid, amino acid, mannite, carotene, trigonelline, adenine, uronic acid and trace elements such as zinc and chromium, has the effects of lowering blood sugar and lipid, detoxifying, protecting livers and resisting aging as long as the pumpkin vermicelli is often eaten and is the first choice for the diabetics.

The invention relates to a biological pesticide composition, in particular to a fungus and plant extract pesticide composition. An insecticidal composition containing metarrhizium anisopliae and veratrine is characterized by mainly comprising the metarrhizium anisopliae and the veratrine, wherein the metarrhizium anisopliae and the veratrine are mixed according to the weight ratio being (1-20):(10-2). The metarrhizium anisopliae is metarrhizium anisopliae conidia, and the content is 5-40 billion spores/gram. The veratrine is a pure substance or a mixture containing veratrum total alkali. The insecticidal composition is environmentally friendly, and can expand the insecticidal spectrum of biological pesticides, be higher in insecticidal effect and longer in lasting period, and reduce the use frequency of pesticides.

The present invention relates to a synergistic composition for the treatment of diabetes in a subject in need thereof, said composition-comprising Trigonelline of concentration ranging between 20 to 30%, amino acids of concentration ranging between 20 to 60%, and soluble fiber of concentration ranging between 10 to 60%, optionally along with pharmaceutically acceptable additives, a process thereof and also, a method of treating diabetes.

The invention discloses a making method of coffee leaf tea. The making method includes: using coffee leaves and/or tender leaf tips as raw materials, preprocessing, fumigating, rolling, drying and packing, to be more specific, cleaning the raw materials in a graded manner, processing in inert gases under normal temperature for 3-9 hours, using steam of 110-120 DEG C to fumigate the raw materials for 1-5 minutes, using a tea leaf rolling machine to roll the raw materials at 40-90 DEG C for 4-8 minutes to obtain targets, drying at 90-125 DEG C for 45-75 minutes, and vacuum packing. The making method has the advantages that the coffee leaves and/or tender leaf tips are used as the raw materials, the nutrient contents such as caffeine, chlorogenic acid and trigonelline rich in the coffee leaves and/or tender leaf tips are reserved sufficiently, the coffee leaf tea is beneficial to human bodies, has functions such as moderate stimulating, beatifying and aging delaying, and unique in flavor; the coffee leaves can be used as tea leaves and also be used as blending and adding raw materials of beverage.

A nutritional composition comprising at least an effective amount of trigonelline or derivative of trigonelline and an effective amount of tropane alkaloids, wherein the ingredients act substantially simultaneously improve the consistency of muscular contractions with respect to applied force and relaxation cycles over time and reduce the onset of central fatigue. A method of same is also provided.

The invention relates to a medicament of a heterocyclic system containing a hexatomic ring using carbon as heterocyclic atoms, in particular to application of trigonelline in preparation of a medicament for preventing and treating diabetes and complication thereof. The medicament consists of 1 to 99.5 percent of trigonelline and pharmaceutically acceptable auxiliary materials. The medicament is an oral preparation such as tablets or capsules.

The invention relates to an application of probiotics in fermented coffee preparation, and belongs to the technical field of coffee. The invention relates to preparation method of coffee beans with special flavor and/or improved quality for consumption. The preparation method of the coffee beans with special flavor and/or improved quality for consumption comprises usage of a kind of probiotics capable of promoting formation of special flavor and/or improved quality of coffee beans. The fermented coffee beans prepared by the preparation method can be used for preparing instant coffee, coffee beverage composition and other coffee foods with special flavor and/or low caffeine purity. According to the preparation method, raw coffee beans are treated with probiotics so that a special flavor isproduced by the coffee beans after baking; and the baked coffee beans are also easy for intestinal absorption. Tests have proven that the coffee beans treated with the probiotics have effectively reduced caffeine content and trigonelline content, as well as increased chlorogenic acid content. In addition, fragrance is also produced by precursors which produce aroma and taste after baking. The invention further relates to a method for preparing the fermented coffee. Fermentation of raw coffee beans is also disclosed in one embodiment of the invention. In the embodiment, specific fermentation combination and temperature condition are required.

The invention discloses a preparation method of pumpkin wine. The method obtains the pumpkin wine through the steps of variety selection, sowing and seedling raising, planting in a field, field management, punching for filling wine and harvesting and storage. According to the method disclosed by the invention, through photosynthesis and day-night temperature difference, the substances such as protein, fat, carbohydrates, calcium, phosphorus, iron, carotene, riboflavin, nicotinic acid, ascorbic acid, citrulline, arginine, asparagine, trigonelline, adenine, glucose, mannitol, pentosan and pectin in pumpkin are mixed in the wine. The pumpkin wine contains polysaccharides, carotenoid, mineral elements, amino acids, active protein and lipid substances, and has the functions of removing toxin, protecting gastric mucosa, helping digestion, preventing diabetes, reducing blood glucose, eliminating cancerogenic substances and promoting growth and development.

The invention provides a method for simultaneously detecting nine chemical components in quisqualis indica, and relates to the technical field of analysis and detection. The method comprises the following steps: extracting quisqualis indica by adopting an alcohol-water solution to obtain a to-be-detected sample solution; and then performing UPLC-MS/MS. According to the invention, a to-be-detectedquisqualis indica sample is pretreated through a UPLC MS/MS method, qualitative identification can be rapidly achieved while gradient elution conditions are controlled, nine chemicals (trigonelline, vanillic acid, ferulic acid, syringic acid, catechinic acid, ellagic acid, malic acid, gallic acid and methyl gallate) in the quisqualis indica medicinal material are quantitatively detected, and reliable experimental data are provided for development and utilization and quality evaluation of the quisqualis indica medicinal material. The detection method provided by the invention has the advantagesof simple operation, fast analysis time of only 8min, fast separation speed and high sensitivity, makes up the blank of nine chemical detection methods in quisqualis indica at present, and is worthyof popularization and application.

The invention provides a method for measuring trigonelline hydrochloride in trigonella tincture, and is characterized in that: a sample passes through a filter membrane of which the thickness is 0.45 microns, and is directly fed and detected by using a NH2 column as an analysis column, and water and acetonitrile as mobile phases by a high performance liquid chromatography, wherein a detector is a diode array detector. The method for measuring the trigonelline hydrochloride in the trigonella tincture specifically comprises the following steps of: (1) drawing a standard working curve: preparing standard solution at at least five concentrations of a trigonelline hydrochloride standard substance, detecting the content of the trigonelline hydrochloride standard substance by using a high performance liquid chromatograph, drawing the standard working curve and calculating to obtain a regression equation of the standard working curve; and (2) preparing a sample: directly injecting the sample after the trigonella tincture passes through the filter membrane of which the thickness is 0.45 microns, respectively substituting the obtained peak areas into the regression equation of the standard working curve, and calculating to obtain the content of the trigonelline hydrochloride in the sample. The method has the advantages that: the pre-treatment step is simple, and the repetitiveness and the recovery rate are high, so the method is applicable to analysis of a large batch of samples; and the blank of analysis and detection of the trigonelline hydrochloride in the trigonella tincture is filled.

The invention belongs to the technical field of daily chemical articles and particularly relates to weight-reducing emulsion and a preparation method thereof. The weight-reducing emulsion is mainly prepared from, by mass, 15-20% of glycerol, 5-10% of propylene glycol, 1-3% of polyoxyethylene oleate, 4-6% of vitamin C, 3-5% of sodium alginate, 1-2% of potassium citrate, 1-2% of lentinan, 0.5-1% of trigonelline, 1-2% of tartronic acid, 1-2% of capsicin, 0.8% of sodium chloride and 45-65% of water. The weight-reducing emulsion can directly act on a fat part, and after the emulsion is continuously used for 1-3 months, the subcutaneous fat content is reduced and skin becomes firm.

The invention discloses a compound biological insecticide of veratine and chlorobenzamide. The compound biological insecticide consists of the veratine and the chlorobenzamide of which the mass ratiois 1:20-20:1. The invention further discloses the application of the compound biological insecticide to the prevention and the control of vegetable pests. A compound of the veratrine and the chlorfenamide has a synergistic effect on the insecticidal activities of aphid and plutella xylostella. The compound biological insecticide disclosed by the invention is suitable for the prevention and the control of lepidopteran pests such as aphids, Plutella xylostella and Spodoptera litura on vegetable crops.

The invention relates to a process for synthesizing trigonelline hydrochloride from dimethyl carbonate and nicotinic acid. The process comprises the following steps of: reacting the nicotinic acid and the dimethyl carbonate in the presence of a catalyst K2CO3, after completely reacting, filtering and removing the K2CO3 and other undissolved substances, and decompressing, concentrating and removing the solvent, namely the dimethyl carbonate; dissolving the remained solid by using 4 mol/L of hydrochloric acid, adjusting pH to 2-3, and decompressing and removing the solvent; dissolving the remained solid by using proper absolute methanol, and filtering and removing the undissolved substances after standing; concentrating and crystallizing the filtrate, filtering to obtain solid, re-crystallizing the solid by using ethanol with a certain concentration, and obtaining the product. According to the process disclosed by the invention, the green methylated reagent, namely the dimethyl carbonate, for substituting the methylated reagents, such as dimethyl sulphate, halomethane and the like, which are poisonous on human bodies and risky on the environment, is reacted with the nicotinic acid; the green trigonelline hydrochloride is synthesized; the process condition is simple; the conversion rate of the nicotinic acid is high; the average substitution degree of the product is high; and the method is environment-friendly.

The invention discloses a combination increasing the yield of alfalfa and improving the quality of alfalfa and application thereof. The combination used for preparing rhizobia culture media is composed of active components of components A and components B, the components A are prepared from stachydrine or hydrochloride of stachydrine, and the components B are prepared from trigonelline or hydrochloride of trigonelline. Alfalfa seed rhizobia seed coatings prepared from the combination used for preparing the rhizobia culture media increase the yield of medicago sativa L.cv.Zhongmu No.1 and improve the quality of the medicago sativa L.cv.Zhongmu No.1. Raw materials of the rhizobia seed coatings for the medicago sativa L.cv.Zhongmu No.1 are easy to obtain, the coating mode is easy to operate, coated seeds are suitable for storage, transportation and sowing, and the coating mode can provide favorable infection time and space for rhizobia infection root system inoculation.

The invention provides a method for separating and purifying trigonelline, chlorogenic acid and caffeine, belongs to the field of extraction and separation of natural products, and particularly relates to a method for separating and purifying three components, namely trigonelline, caffeine and chlorogenic acid, in a coffee extracting solution. The method comprises the following steps: starting from a coffee extracting solution, adjusting the pH value of the extracting solution to 3-6, loading a sample to polyamide adsorption resin, eluting with water to obtain an alkaloid component, and desorbing with an alcoholic solution to obtain a phenolic acid component mainly comprising chlorogenic acid; concentrating the alkaloid component properly, adjusting the pH value to 7, loading a sample onto non-polar macroporous adsorption resin and eluting with water, obtaining a trigonelline component, and then desorbing the trigonelline component with an alcohol solution to obtain a caffeine component. The process is simple in operation step, short in production cycle, free of toxic and harmful reagents and complex production equipment, and beneficial to expanding the production scale.

The invention provides a macro-scale biological nylon fiber containing active ingredients of coffee and a preparation method thereof, and belongs to the technical field of fiber materials. The preparation method includes the following steps: crushing coffee beans, extracting active ingredients with antibacterial and antiviral effects, such as chlorogenic acid, caffeine and trigonelline, loading the extracted active ingredients on porous nano-particles, and modifying; and finally, carrying out melt spinning on the modified porous nanoparticles containing the active ingredients of the coffee and chinlon 66 slices to obtain the macro-scale biological chinlon fiber containing the active ingredients of the coffee. According to the method, the compatibility between the active ingredients of an extract and polyamide fibers is good, the active ingredients of the extract can be stably distributed in the polyamide fibers, and the immobilization degree is good; in a preparation process, the active ingredients are protected by a porous nanoparticle molecular nest technology, so that the loss caused by a high-temperature process is reduced, and the modification effect is good; the prepared macro-scale biological chinlon fiber containing the active ingredients of the coffee has a good virus inhibition effect.

The invention relates to the field of food processing, and especially relates to a drying method of coffee beans. According to the drying method, the coffee beans are dried by a microwave vacuum drying method; and technological parameters are optimized in the drying method, so that water contents of the dried coffee beans are no higher than 12.5%, the drying time is no longer than 3 hours and the mildew rates are 0, which are all obviously lower than the prior art (p & lt; 0.05). Moreover, losses of flavor substances and nutrients are relatively small, so that the flavor substances, including caffeine, trigonelline and the like, as well as nutrients, including fat, proteins and the like, in the coffee beans can be effectively preserved.

The invention relates to a gel capable of preventing and treating decayed dry pests. The gel is prepared from the following components: 20-30g of an agent A, 40-60mL of an agent B, 10-20mL of an agentC, 30-50mL of an agent D, 1-1.5g of an agent E and water supplemented till the total volume reaches 1000mL, wherein the agent A is selected from one or several of xanthan gum, sodium alginate and locust bean gum; the agent B is selected from one of 1,3-propylene glycol or sorbitol; the agent C is absolute ethyl alcohol; the agent D is selected from one of 25% chlorbenzuron suspensoid, 1.2% nicotine sophocarpidine emulsion, 1% celastrus angulatu emulsion in water or 0.5% veratrine soluble liquid agent; the agent E is selected from one of 50% carbendazol wettable powder or 70% thiophanate-methyl wettable powder. The invention also relates to application of the gel to prevention and treatment of the decayed dry pests. The gel is excellent in effect of preventing and treating the decayed drypests, is non-toxic and tasteless, and is safe to natural enemies and environment of the decayed dry pests.

The present invention relates to a method for processing coffee cherries using deep seawater and microorganisms. The method processes coffee cherries by using microorganisms and deep seawater, such that the amount of defective coffee cherries are reduced, a processing time of green beans is shortened, and a content of trigonelline which is a precursor of an aroma component of coffee is increased.

The invention discloses a soybean trigonelline synthase (GmTS) as well as a coding gene and application thereof. The protein serving as a substrate of the trigonelline synthase has high specificity; Na<+>, K<+>, NH4<+>, Mg<2+>, Mn<2+> and Ca<2+> do not have significant influence on the enzyme activity; heavy metal ions such as Zn<2+>, Cu<2+>, Fe<2+> and Fe<3+> can significantly inhibit the enzyme activity; when the pH value of the protein is 6.0-7.0, the enzyme activity of the trigonelline synthase is relatively high. The protein is the one of the following (1) or (2): (1) protein shown by SEQ ID No. 6; and (2) proteins which are obtained by substituting and/or deleting and/or adding one or a few amino acid residues to an amino acid sequence shown by SEQ ID No. 6 and have the same function. The trigonelline synthase and the coding gene thereof have wide application prospect in the fields of soybean molecular assisted breeding, human health care and the like.

The invention discloses a stem cell cryopreservation liquid as well as a preparation method and a use method thereof. The stem cell cryopreservation liquid is prepared from 0 to 20 percent of DMSO, 1to 2 percent of glycerol, 1 to 2 percent of trehalose, 2 to 5 percent of ethanol, 0.05 to 0.07 percent of N-dodecanoyl sarcosine, 0.7 to 1.0 percent of calcium phosphate, 0.03 to 0.06 percent of polyporus acid C, 0.02 to 0.05 percent of trigonelline, 0.01 to 0.04 percent of 1, 8-cineole, and the balance of DMEM culture medium. After the stem cell cryopreservation liquid is used for cryopreservation, the stem cell resuscitation rate reaches 90% or above, and compared with non-cryopreserved cells, the proliferation speed is not obviously reduced and even increased.

Coffee berry wine and a preparing method thereof are disclosed. The fermented coffee berry wine the alcohol content of which is 9-12% and the total sugar content is less than 1% is prepared by juicingfresh and mature coffee berry flesh with peel, degumming the obtained juice, adjusting the sugar content of the degummed juice by utilizing cane sugar, then adding an activating enzyme into the juice, and performing secondary fermentation. The coffee berry wine is rich in nutrition, contains a high amount of caffeine, chlorogenic acid, trigonelline, vitamins, and the like, and also contains anthocyanins and the like. The coffee berry wine is bright in color and good in palatability. The coffee berry wine can build one's body by frequent drinking, and can further prevent oxidation and delay senescence.

The invention belongs to the technical field of researches and development of novel pesticides, and particularly relates to a veratrine containing insecticide composition. The veratrine containing insecticide composition comprises the effective ingredients of veratrine and Liuyang mycin, and the weight ratio of the veratrine to the Liuyang mycin is 1:40 to 40:1. The insecticide composition comprises the effective ingredients having different function mechanisms, the synergistic interaction is obvious, the use amount of a single dosage is reduced; the veratrine containing insecticide composition is active for all states of the pest mites and effectively slows down the production of resistance of the mites; and the composition adopts the biogenic pesticide, and the environment pollution is relatively low.