17 results about "Quinic acid" patented technology

The present application provides a method for extracting complex organic acid from the waste liquid of the extract of Rubus corchorifolius L.f. The waste liquid of the column chromatography effluent of the Rubus corchorifolius L.f. extract is taken, the specific gravity of the secondary waste liquid is adjusted to obtain the column loading liquid, the column loading liquid is passed through a resin column A, water washing is performed, the column effluent and the water washing liquid are collected to obtain a combined liquid 1, the combined liquid 1 is passed through a resin column B, water washing is performed, reagent C is used for elution, and the eluate is collected. The combined liquid 2 is obtained by passing through a resin column D and collecting the effluent and the water washing liquid, vacuum concentration is performed, crystallization is performed, and filtration is performed to obtain quinic acid.

The present application relates to plant active ingredient extraction technology, particularly to a method for extracting quinic acid in cranberry, specifically, the method comprises the following steps: crushing the cranberry, mixing the crushed cranberry with water to form a slurry, extracting the slurry under low temperature and ultrasonic wave, filtering the slurry after the extraction is completed to remove solid residues, obtaining a filtrate, adding activated carbon to the filtrate for adsorption, adding a desorption agent to the activated carbon for desorption after the adsorption is completed, obtaining quinic acid extract, concentrating the quinic acid extract, and freeze-drying the concentrated quinic acid extract to obtain quinic acid, the extraction device of the present application has high degree of automation and can improve the crushing effect of the cranberry.

The invention provides application of a TaPAL8 gene in positive regulation of phenolic acid content of wheat, and belongs to the technical field of gene engineering. Wide targeted metabolome detection is carried out on wheat whole growth period materials by utilizing an HPLC-MS / MS technology, specific accumulation of various phenolic acids in stalks is highly related to expression of multiple phenylpropane pathway genes, one gene is annotated as phenylalanine ammonialyase, the gene number is TraesCS6D01G212200, and the gene is named as TaPAL8. According to the embodiment of the invention, the gene TaPAL8 is transformed into a wheat variety Fielder to obtain a transformed plant with overexpression TaPAL8, and in the overexpression mutant, the content of various acyl modified quinic acid substances in stalks and grouted grains is remarkably increased.

The invention relates to the field of biological medicine, in particular to application of a quinic acid derivative. The quinic acid derivative comprises one or two of 3-O-p-coumaroyl quinic acid and 4-O-p-coumaroyl quinic acid, and the quinic acid derivative comprises one or two of 3-O-p-coumaroyl quinic acid and 4-O- The applications include treatment and / or prevention of depression. The quinic acid derivatives 3-O-p-coumaroyl quinic acid and 4-O-p-coumaroyl quinic acid can be used for improving the behavioristics of depressive mice; the compound can significantly increase dopamine and noradrenaline in the brain of a depressive mouse, reduce the levels of inflammatory factors IL-6 and TNF-alpha in the brain of the depressive mouse, increase the intestinal microbial diversity of the depressive mouse and significantly increase the BDNF level in the brain of the depressive mouse, and can be used as a drug for treating and / or preventing depression.

A method for treating tobacco material, the method comprising: providing a tobacco material, fermenting the tobacco material to obtain fermented tobacco material, the fermenting step including: # incubating the tobacco material under anaerobic conditions; # stopping the fermentation when at least one of the following conditions is satisfied: # the content of Lactic Acid is more than 10 times, preferably more than 20 times, more preferably more than 50 times, more preferably more than 70 times, preferably more than 80 times an initial amount of Lactic Acid in the tobacco material, # the content of Reducing Sugars is lower than 0.5, preferably lower than 0.4, more preferably below 0.2, more preferably below 0.1 an initial amount or Reducing Sugars in the tobacco material, # the content of Indole-3 Lactic Acid is more than 5 times, preferably more than 10 times, preferably more than 20 times, an initial amount of Indole-3 Lactic Acid in the tobacco material, # the content of caffeic acid is more than 4 times, preferably more than 10 times, preferably more than 20 times, an initial amount of caffeic acid in the tobacco material. # the content of quinic acid is more than 2 times, preferably more than 4 times, an initial amount of quinic acid in the tobacco material, # the content of asparagine is lower than 0.5, preferably lower than 0.4, preferably lower than 0.3 an initial amount or asparagine in the tobacco material, # the content of Glutamine is lower than 0.5, preferably lower than 0.4 an initial amount or Glutamine in the tobacco material, # the content of L-Ornithine is more than 10 times, preferably more than 20 times, preferably more than 50 times, preferably more than 100 times an initial amount of L-Ornithine in the tobacco material, # the content of L-Leucine is more than 2 times, preferably more than 4 times, an initial amount of L-Leucine in the tobacco material, # the content of L-Lysine is more than 2 times, preferably more than 6 times, an initial amount of L-Lysine in the tobacco material, # the fermentation index is more than 50, preferably more than 100, more preferably more than 250, more preferably more 400, wherein the fermentation index is obtained dividing the ratio between the content of Lactic Acid in the treated tobacco material and the content of Lactic Acid in the non-fermented tobacco material by the ratio between the content of Reducing Sugars in the treated tobacco material and the content of Reducing Sugars in the non-fermented tobacco material.

The invention relates to a polyolefin adhesive for stainless steel and a preparation method thereof, and belongs to the technical field of adhesives. According to the invention, quinic acid and shikimic acid are firstly used for modifying sodium carbonate, then modified calcium carbonate and ramie fiber are used as fillers for filling so as to construct an efficient stress transfer system, and meanwhile, maleic anhydride grafted polyethylene is also added for assisting filling of the fillers, so that the impact resistance of the prepared adhesive is effectively improved; besides, malonaldehyde is added to reduce the activity of molecules in the material and limit molecular motion, vinyl neodecanoate with a side group is doped to increase the steric hindrance of molecular motion in the system, and hexanediol diglycidyl ether is doped to increase the density of a cross-linked network of the system, so that the composite material has excellent mechanical properties. The adhesive prepared by the method has relatively high peel strength.

The invention provides a method for synthesizing quinic acid by using escherichia coli whole cells, and belongs to the technical field of microbial metabolism engineering. According to the method for synthesizing quinic acid provided by the invention, through specific genetic engineering modification and fermentation process optimization, efficient bioconversion with renewable saccharides as raw materials is realized, the production process is mild in conditions, free of harmful chemical reagents, environment-friendly and relatively low in energy consumption, and the production cost and environmental protection pressure are effectively reduced. The yield of the quinic acid product prepared by the method can reach 17.1 g / L, the quality is stable, a reliable raw material source is provided for the fields of medicine, food, health care products and the like, and green manufacturing and industrial development of related high-added-value natural products are promoted.

The present application relates to a composition containing chlorogenic acid, caffeic acid, and quinic acid. The polyphenol-containing composition of the present application may be used for reducing an off-taste or off-flavor. In addition, the polyphenol-containing composition of the present application may be used as a preservative of a product, in particular an antioxidant or browning inhibitor of a product.

The present application relates to the technical field of textiles, and discloses a plant dyeing and finishing process, which comprises the following steps: S1, pretreating wool / cashmere fabric to obtain pretreated fabric; S2, sequentially performing piece dyeing, opening, first drying, raising, first shearing, first wet brushing, second drying, second shearing, continuous steaming, second wet brushing, third drying, closed steaming, and steaming treatment on the pretreated fabric to obtain dyed and finished fabric; in step S1, the pretreatment agent used in the pretreatment comprises component alkylphenol polyoxyethylene ether, chitosan quaternary ammonium salt, and quinic acid. The above technical solution solves the problem of poor dyeing durability of plant dyeing and finishing fabric in the prior art.

The invention belongs to the technical field of medicine detection, and particularly relates to a detection method of a traditional Chinese medicine preparation for treating recurrent oral ulceration, the traditional Chinese medicine preparation is prepared from traditional Chinese medicinal materials including inula cappa, liquorice, pearl and borneol, and the detection method comprises the items of character determination, identification, inspection, characteristic spectrum determination and content determination. Wherein the identification comprises microscopic identification and thin-layer chromatography identification of inula cappa, liquorice and borneol; the characteristic chromatogram is characterized by adopting an HPLC (High Performance Liquid Chromatography) method for active ingredients contained in inula cappa and liquorice; and content determination is to detect the contents of glycyrrhizic acid and 3, 4-O-dicaffeoyl-quinic acid in the product. According to the detection method established by the invention, the detection requirement on the product quality is improved, establishment of a perfect quality control system of the product is facilitated, and the medicine quality and the clinical curative effect are guaranteed.

The invention relates to the technical field of extraction and separation of plant functional components, in particular to a method for extracting multiple functional components from ginkgo biloba leaves, which is characterized in that alcohol-soluble and water-soluble functional components are respectively extracted in parallel by adopting an organic solvent and water by researching the solubility difference of the components in the ginkgo biloba leaves. The core of the method is that pigment, flavone, lactone, sulfated polysaccharide, quinic acid and shikimic acid are sequentially or synchronously separated from different extraction paths through a series of steps of solvent distribution, filtration, ion exchange resin column chromatography and the like by taking an extraction and purification process of flavone and lactone as a center. The method disclosed by the invention realizes maximum comprehensive utilization of ginkgo leaf resources, is simple and efficient in process flow, high in product extraction rate and purity, low in cost, green and environment-friendly, is easy to enlarge industrial production, and has remarkable economic and social benefits.

The invention relates to the technical field of chlorogenic acid yield improvement. The hydroxycinnamyl coenzyme A quinic acid transferase mutant provided by the invention is obtained by mutating the 214th amino acid S of wild type HQT into G and mutating the 224th amino acid S into R. The invention also provides a preparation method of the hydroxycinnamyl coenzyme A quinic acid transferase mutant. The chlorogenic acid production activity of the mutant provided by the invention is improved.

The invention relates to a coffee baking method, which comprises the following steps of: selecting a plurality of coffee beans, preheating the coffee beans in a bean baking bin, adding the coffee beans to heat the coffee beans to a temperature return point, continuously heating the coffee beans to enable the internal moisture temperature to be at an evaporation edge of 90-100 DEG C so as to soften coffee bean fibers, and then baking the coffee beans according to the temperature rise rate in the previous step. The method comprises the following steps: heating coffee beans at a temperature rise rate of + / -1.5 DEG C / min until the water content of the coffee beans is lower than 5%, controlling the temperature to be in a primary explosion state, carrying out a dehydration reaction on chlorogenic acid in the coffee beans, converting the chlorogenic acid into chlorogenic acid lactone, stopping baking before secondary explosion and before degradation of the chlorogenic acid lactone, and taking out the coffee beans to complete baking. Therefore, the water content of the coffee beans is reduced as much as possible, quinic acid and caffeic acid are prevented from being generated due to the fact that chlorogenic acid enters a hydrolysis reaction, meanwhile, the baking degree is controlled to prevent chlorogenic acid lactone from being degraded to generate a large number of compounds, and good-flavor coffee created by the chlorogenic acid lactone is brewed.

The application discloses a method for screening and determining active ingredients of Wuji drink, and a method for determining the content of active ingredients of Wuji drink, which comprises the following steps: adding methanol as an extraction solvent to a Wuji drink dry extract, performing ultrasonic extraction, filtering, and obtaining a test product solution; preparing 3-O-p-coumaroyl quinic acid control product solution, DL-syringaresinol control product solution and methyl ophiopogonanone B control product solution by using methanol; precisely sucking the control product solution and the test product solution respectively; determining the peak area by using UPLC-MS / MS method; and calculating the content of the three components. The application firstly establishes a method for determining the content of three active components, i.e., 3-O-p-coumaroyl quinic acid, DL-syringaresinol and methyl ophiopogonanone B, in a classic medicinal diet Wuji drink dry extract, wherein the 3-O-p-coumaroyl quinic acid is an effective component for determining the content of Wuji drink, and the content determination method meets the relevant regulations for health food declaration.

The invention relates to the technical field of food biotechnology and fermentation engineering, and relates to kiwi fruit vinegar, a preparation method and application thereof, and a kiwi fruit vinegar beverage. Alcohol fermentation and acetic fermentation processes are optimized through a response surface method, the optimal brewing conditions and process parameters are determined, and the alcoholic strength and the acid yield are remarkably improved. The ethanol yield, the alcoholic strength and the growth speed of the non-saccharomyces cerevisiae Td7 used in the test are all superior to those of saccharomyces cerevisiae DV10, and the degradation rate of quinic acid is 27.93% and is obviously higher than that of DV10. The Td7 shows a relatively strong competitive advantage in sequential fermentation, occupies a dominant position in fermentation, and is relatively short in fermentation period. The acid yield of the acetic acid bacteria C5-1 is obviously higher than that of commercial strains. The total acid of the kiwi fruit vinegar cooperatively brewed by Td7 and C5-1 is high and is 54.10 g / L, the content of tartaric acid, citric acid and succinic acid in the fruit vinegar is remarkably reduced, the content of acetic acid is remarkably increased, and the acidity and the sense of equilibrium are enhanced.

Consumable products are provided. The consumable products include: (a) at least one sweetener, and (b) a sweetness modifier comprising an extract or fraction from Uncaria tomentosa or at least one caffeoylquinic acid or tormentic acid compound. The at least one sweetener is present in a sweetening amount.

Disclosed is a technique for constructing optogenetic amplifier and inverter circuits utilizing transcriptional activator / repressor pairs, in which expression of the transcriptional activator or repressor, respectively, is controlled by light-controlled transcription factors. This system is demonstrated utilizing the quinic acid regulon system from Neurospora crassa, or Q System, a transcriptional activator / repressor system. This is also demonstrated utilizing the galactose regulon from Saccharomyces cerevisiae, or GAL System. Such optogenetic amplifier circuits enable multi-phase microbial fermentations, in which different light schedules are applied in each phase to dynamically control different metabolic pathways for the production of proteins, fuels or chemicals. The orthogonal nature of the Q and GAL systems enable the co-expression of amplifier and inverter circuits to simultaneously amplify and invert the response of light-controlled transcriptional controls over different sets of genes in the same cell.