8 results about "Injection volume" patented technology

The invention provides a method for detecting related substances of sucrose octasulfate salt auxiliary materials, and particularly relates to the field of medicines. An SCX cation exchange chromatography filler and a C18 filler are mixed according to the ratio of (0.8-1.2): (3-5) to serve as a filling agent; the specification of a chromatographic column is CAPCELLPAK CR C18 + SCX 5 [mu] m, 250 * 4.6 mml.D.; 18-22 mM / L ammonium formate and 0.08-0.12% formic acid are used as a mobile phase A, and acetonitrile is used as a mobile phase B; a detector is CAD, the spray temperature is High, the collection frequency half-peak width is 0.1-0.2 min, the column temperature is 28-32 DEG C, the flow rate is 0.8-1.2 ml / min, and the sample injection volume is 4-6 [mu] l; wherein acetonitrile and water in a ratio of (3-5): (5-7) are used as diluents, and gradient elution is carried out; the method provided by the invention can effectively separate and accurately analyze each impurity, has strong specificity and good separation degree, and can effectively control the product quality.

The invention provides a detection method of black-bone chicken melanin oxidation products, and belongs to the technical field of chemical detection. The detection conditions of liquid chromatography in the detection method provided by the invention are as follows: the sample size is 0.9-1 [mu] L, a mobile phase A is a formic acid aqueous solution with the volume fraction of 0.05-0.15%, a mobile phase B is methanol, gradient elution is adopted, the detection time is 1-2 hours, and the detection wavelength is 250nm. The gradient elution conditions are as follows: the volume percentage content of the mobile phase B in 0-3 min is 5%-25%, the volume percentage content of the mobile phase B in 3-4 min is 25%-95%, the volume percentage content of the mobile phase B in 4-5.5 min is 95% B, the volume percentage content of the mobile phase B in 5.5-6 min is 95%-5%, and the volume percentage content of the mobile phase B in 6-8 min is 5% B. According to the present invention, the ultra-high performance liquid chromatography-mass spectrometry detection conditions are controlled, such that the detection accuracy is improved, the sample injection amount is reduced, the elution time is shortened, and the time and the sample consumption are saved.

The invention relates to the technical field of chemical gas chromatography analysis and detection, in particular to a device and method for chlorosilane detection, and the method comprises the following steps: inserting a needle head of an injector into a chlorosilane sample bottle, pulling back a push rod at a pulling-back speed of v, and extracting a sample of V1; the needle head of the injector faces upwards, bubbles float upwards to the top and are pushed and injected until the bubbles are discharged, and the needle cylinder is filled with the sample; according to the method, the needle head is inserted into a sample inlet of a heated gas chromatograph for detection, and the problem of inaccurate sample injection volume caused by bubbles generated by rapid volatilization of chlorosilane is effectively avoided through the steps of slow liquid extraction and bubble discharge, so that the accuracy of gas chromatography detection data and the repeatability of an experiment are remarkably improved; after the injection is finished, the specific high temperature of the sample inlet is combined, so that the chlorosilane sample is ensured to be instantly and completely gasified, the needle head is prevented from being remained or the sample is prevented from being volatilized in advance in the needle, the detection integrity is ensured, and the cross contamination of the sample inlet and a chromatographic column is avoided.

The application discloses a detection method for determining the residual amount of easily hydrolyzed impurities in apremilast, which comprises the following steps: using a high performance liquid chromatograph, adopting polysaccharide derivatives as a filler (CHIRALPAK IH, 4.6mm*150mm, 5um), adopting a n-hexane-tetrahydrofuran (65:35) mobile phase, a flow rate of 1.0ml per minute, a column temperature of 30 DEG C, a detection wavelength of 230nm, and a sample injection volume of 50ul. The application adopts the polysaccharide derivative filler to determine the easily hydrolyzed impurities in the apremilast, has high separation efficiency, strong anti-interference ability, fast analysis speed and high detection sensitivity, and can effectively control the quality of the apremilast by determining the amount of the easily hydrolyzed impurities in the apremilast.

The invention discloses a high-pressure sampling method for gas chromatographic analysis. The method comprises the following steps: (1) collecting a sample through a sampling steel cylinder; the sample steel cylinder is connected with the quantitative sample injection valve through the switching valve; (2) cleaning a solvent cavity and a sample injection cavity of the quantitative sample injection valve by using a solvent through a cleaning pipeline, and purging the cleaned solvent to a vaporizing chamber through a GC system carrier gas and a sample injection needle; then quantitatively filling the sample injection cavity and the solvent cavity with the solvent again; (3) purging the sample injection cavity by using inert gas through the purging pipeline, wherein the solvent is continuously reserved in the solvent cavity in the purging process; (4) opening an inlet / outlet valve of the sampling steel cylinder, and quantitatively filling the sample into the sample injection cavity; and (5) sequentially blowing the sample in the sample injection cavity and the solvent in the solvent cavity into a vaporizing chamber through a sample injection needle by virtue of GC system carrier gas, and entering a chromatographic column for gas chromatographic analysis. According to the invention, the problem of sample injection distortion can be thoroughly solved, and the consistency and fidelity of the sample injection amount are ensured.

A liquid injection device, a liquid injection method, and a microfluidic system are disclosed. The liquid injection device (100) has an injection mode and a replenishment mode. In the injection mode, a transmission liquid is injected into the first cavity (111), which acts on the first surface (311) and drives the second sliding part (32) to slide through the first connecting part (33), so that the reaction liquid in the fourth cavity (212) or the downstream section of the fourth cavity (212) is injected into the reaction device (200). In the replenishment mode, a transmission liquid is injected into the second cavity (112), which acts on the second surface (312) and drives the second sliding part (32) to slide through the first connecting part (33), so that the reaction liquid injected into the fourth cavity (212) or the downstream section of the fourth cavity (212) is contained therein. The area of ​​the transmission liquid acting on the first surface (311) is larger than the area of ​​the transmission liquid acting on the second surface (312), so slow injection and rapid replenishment can be achieved. Slow injection is beneficial for precise and micro-control of the injection volume.

This invention relates to a method for determining the encapsulation efficiency of liposome injection solutions. The method comprises the following steps: 1) preparation of a blank solution; 2) preparation of a test solution; 3) determination: injecting the blank solution and the test solution into a high-performance liquid chromatograph (HPLC) to obtain a chromatogram, and calculating the encapsulation efficiency of the test solution based on the peak area of ​​the chromatogram. The chromatographic conditions for the HPLC are as follows: instrument: HPLC system, VWD or DAD; column: Thermo Hypersil BDS C8; mobile phase: sodium hexanesulfonate solution: acetonitrile = 75:25; column temperature: 40℃; flow rate: 1.0 ml / min; detection wavelength: 255 nm; injection volume: 10 μl; run time: 10 min.

The invention relates to the technical field of drug analysis, and discloses a method for determining impurity I in bumetanib injection, which comprises the following steps: using a reverse high performance liquid chromatograph and an ultraviolet detector, taking ACE Excel C18-PFP as a chromatographic column, taking 0.1% phosphoric acid aqueous solution as a mobile phase A, taking methanol-acetonitrile (40: 60) as a mobile phase B, adopting a gradient elution procedure, and determining the impurity I in the bumetanib injection according to the detection wavelength of 220nm, the sample injection volume of 10mu L and the flow velocity of 1.0 mL / min. The column temperature is 30 DEG C; the content of the impurity I in the bumetanib injection can be accurately detected, the blank of no impurity I detection method report is filled, and method verification is carried out. The method designed by the invention can be used for separating and accurately determining the impurity I in the bumetanib injection, and is high in specificity, high in sensitivity and good in precision and accuracy; and the quality control of the bumetanib injection is effectively enhanced.