14 results about "Carbetocin" patented technology

The invention provides a composition and a method for sow simultaneous delivery and application of the composition and the method, and particularly discloses a composition and a method for shortening the delivery process and improving the live piglet rate according to a sow simultaneous delivery scheme applying vitamin B6, mifepristone, cloprostenol sodium and carbetocin. According to the composition, aiming at the problem that the sow production efficiency is influenced by the reduction of the live piglet rate caused by the simultaneous delivery of sows, the vitamin B6 and the mifepristone are combined on the basis of the delivery drugs cloprostenol sodium and carbetocin, so that a set of new simultaneous delivery scheme is formed; according to the method, the delivery process of the sows is shortened, the live piglet rate of the sows is increased, the method is a method for improving the same-period delivery effect of the sows in batches, and great value is provided for improving the PSY of the sows and promoting batch management of the sows.

The invention relates to a batch production method capable of optimizing the sow delivery induction process and improving the delivery concentration ratio. Comprising the following steps: S1, determining an expected date of confinement of a sow; s2, injecting cloprostenol sodium into the pudendum of the sow in the morning one day before the expected date of confinement; s3, performing intramuscular injection of carbetocin for the sows in the morning of the expected date of confinement. According to the method, potential adverse effects of childbirth induction are deeply studied, the optimal time and dosage for combined use of cloprostenol sodium and beioxytocin are determined, childbirth induction for batch production of sows is optimized, the childbirth time of the sows is concentrated in the working time of a pig farm, the labor efficiency is improved, and the labor intensity is reduced.

This invention discloses a carbetocin injection solution and its preparation process. The raw materials for the carbetocin injection solution include at least carbetocin, methionine, disodium edetate, an antibacterial agent, a pH adjuster, and a solvent. The pH of the carbetocin injection solution is 4.0-4.5. The concentration of carbetocin is 50 μg / ml-80 μg / ml, the concentration of methionine is 10 mg / ml-20 mg / ml, the concentration of disodium edetate is 5 mg / ml-10 mg / ml, and the concentration of the antibacterial agent is 2 mg / ml-3 mg / ml. By controlling the concentrations of carbetocin, methionine, disodium edetate, and the antibacterial agent, and simultaneously adjusting the pH of the injection solution to 4.0-4.5, this invention eliminates the need for nitrogen purging during the preparation process, thereby enabling the prepared carbetocin injection solution to exhibit high antioxidant properties and stability, allowing for long-term storage at room temperature.

The invention discloses a method for detecting related substances of carbetocin injection, and relates to the technical field of drug detection and analysis. The detection method comprises the following steps: preparing a solution; and detecting related substances of the carbetocin injection by high performance liquid chromatography. The detection method provided by the invention avoids the use of ion pair reagents in the mobile phase by selecting a suitable mobile phase and controlling the conditions of the gradient elution program. On the premise of ensuring effective separation of the main peak and impurities, the detection method solves the problems of poor baseline, low response, and short service life of chromatographic column consumables in the existing high performance liquid chromatography system. The method has the characteristics of strong specificity, good precision, high accuracy, simplicity, high efficiency, low cost, etc., and can be applied to the quality control of carbetocin injection. At the same time, the use cost of chromatographic column consumables is greatly reduced, and the method is more suitable for commercial production.

This invention provides a method for determining the content of the antioxidant methionine in carbetocin injection, comprising the following steps: Preparation of the reference solution: Accurately weigh an appropriate amount of methionine reference standard, dissolve it in water, and quantitatively dilute it to prepare a solution containing 1 mg per 1 ml. Preparation of the test solution: Take the product, and the test solution is obtained. Determination method: Accurately measure 10 μl each of the test solution and the reference solution, inject them separately into the liquid chromatograph, record the chromatogram until twice the retention time of the methionine peak, and calculate the methionine content in carbetocin injection by peak area using the external standard method. This invention uses high-performance liquid chromatography (HPLC), and by optimizing the determination method by changing conditions such as the mobile phase ratio and comparing it with traditional detection methods, it demonstrates that this invention is a more stable, effective, and rapid method for determining the methionine content.

The application provides a composition, a method and application thereof for sows to be delivered at the same time, and particularly discloses a composition and a method for sows to be delivered at the same time by using vitamin B6, mifepristone, combined with sodium cloprostenol and carbetocin to shorten the delivery process and improve the live birth rate. The composition of the application aims at the problem that the live birth rate is reduced due to the sows to be delivered at the same time, thereby affecting the production efficiency of sows, and on the basis of the delivery drugs sodium cloprostenol and carbetocin, vitamin B6 and mifepristone are combined to form a new delivery at the same time scheme. The application shortens the delivery process of sows and improves the live birth rate of sows, and is a method for improving the delivery effect of batch sows at the same time, which provides great value for improving the PSY of sows and promoting the batch management of sows.

The invention discloses a carbetocin injection and a preparation process thereof, the carbetocin injection at least comprises the following raw materials: carbetocin, methionine, edetate disodium, a bacteriostatic agent, a pH regulator and a solvent, and the pH of the carbetocin injection is 4.0-4.5; wherein the concentration of the carbetocin is 50 [mu] g / ml to 80 [mu] g / ml, the concentration of the methionine is 10 mg / ml to 20 mg / ml, the concentration of the edetate disodium is 5 mg / ml to 10 mg / ml, and the concentration of the bacteriostatic agent is 2 mg / ml to 3 mg / ml. By controlling the concentrations of carbetocin, methionine, edetate disodium and the bacteriostatic agent and adjusting the pH value of the injection to 4.0-4.5, the prepared carbetocin injection can have higher oxidation resistance and stability without introducing nitrogen in the preparation process, and can be stored for a long time at room temperature.

The invention relates to a method for synthesizing carbetocin using special amino acids, which mainly solves the technical problem that the current synthesis method is not suitable for industrial production. The invention comprises the following steps: synthesizing a peptide fragment (Fmoc-Cys((CH2)3COOH)-Pro-Leu-Gly-NH2) and a peptide resin (NH2-Tyr(Me)-Ile-Gln(Trt)-Asn(Trt)-CTC Resin) containing special cysteine ​​by a standard Fmoc solid phase peptide synthesis method; coupling the peptide fragment with the peptide resin to obtain a fully protected linear carbetocin peptide resin, and then removing Fmoc and fully protecting cleavage to obtain a linear carbetocin with a protecting group in the side chain; and obtaining carbetocin after intramolecular amide bond cyclization and deprotection cleavage. The method is conducive to the large-scale industrial production of carbetocin.

The invention relates to a carbetocin injection isomer detection method, and belongs to the technical field of polypeptide drug impurity analysis. The carbetocin injection isomer detection method comprises the following steps: detecting Ile position isomer impurities in 8-site Leu in a carbetocin raw material by adopting a high performance liquid chromatography; the adopted chromatographic conditions are as follows: octadecyl silane bonded silica gel is taken as a filling agent, acetonitrile-0. 02 mol / L ammonium acetate-0.03 mol / L monopotassium phosphate is taken as a mobile phase A, methanol-acetonitrile is taken as a mobile phase B, and gradient elution is performed; the detection wavelength is 210 to 230 nm; the column temperature is 40-70 DEG C; the flow velocity is 0.5 to 1.2 mL / min; and the sample injection volume is 10-40 [mu] L. The carbetocin injection isomer detection method is high in accuracy and good in sensitivity, repeatability and precision meet requirements, position isomer impurities can be accurately detected, and the product quality can be better controlled.

The invention discloses a detection method of carbetocin injection related substances, and relates to the technical field of drug detection and analysis, the detection method comprises the following steps: preparing a solution; and detecting the carbetocin injection related substances by adopting a high performance liquid chromatography, and the like. According to the detection method provided by the invention, by selecting a proper mobile phase and controlling conditions of a gradient elution procedure, the use of an ion pair reagent in the mobile phase is avoided, and on the premise of ensuring effective separation of a main peak and impurities, the problems of poor baseline, low response, short service life of chromatographic column consumables and the like of an existing high performance liquid chromatography system are solved; the method has the characteristics of strong specificity, good precision, high accuracy, simplicity, high efficiency, low cost and the like, can be applied to quality control of the carbetocin injection, greatly reduces the use cost of chromatographic column consumables, and is more suitable for commercial production.

To provide an improved method for isolating carbetocin so as to produce a large amount of carbetocin having sufficient purity.SOLUTION: The invention provides a method for producing carbetocin in crystalline form, comprising crystallizing carbetocin, and a pharmaceutical composition comprising carbetocin according to the invention.SELECTED DRAWING: Figure 1

The invention relates to a method for synthesizing carbetocin through a double-fragment solid-liquid phase, and mainly solves the technical problems that the purity of crude peptide synthesized through a pure solid-phase long fragment in an existing synthesis method is low, and special cysteine and an expensive deprotection metal reagent are used. According to the technical scheme, the method for synthesizing the carbetocin through the double-fragment solid-liquid phase is characterized in that synthesis of the carbetocin is achieved through chemical coupling and cyclization of two fragments, and the carbetocin is obtained through double-fragment coupling at an Asn site or a Cys site and then cyclization. The synthesis yield and purity are improved, and large-scale industrial production of carbetocin is facilitated.

The present invention relates to a carbetocin injection and a preparation method thereof, belonging to the technical field of pharmaceutical preparations. A stabilizing adjuvant is added to the carbetocin injection. Based on 10 L of the carbetocin injection, the addition amount of the stabilizing adjuvant is 0.25 g to 1.0 g, and the stabilizing adjuvant is selected from at least one of apigenin, catechin, luteolin, troxerutin, puerarin, and baicalin. The present invention discovers that by introducing an appropriate amount of specific natural substances such as puerarin as a stabilizing adjuvant into the carbetocin injection, it can effectively slow down the increase in the impurity content during the storage of the carbetocin injection and reduce the types and dosages of excipients in the prescription. This not only improves the storage stability of the carbetocin injection but also avoids the risks of increased microbial levels and incompatibility with raw materials brought about by the introduction of excessive excipients, thereby enhancing the safety of drug use.

The application provides a carbetocin injection and a preparation method thereof, and belongs to the technical field of preparations. The preparation method is as follows: a pH regulator is used to prepare an aqueous solution, 50 parts by weight of glucose, 10-15 parts by weight of polyethylene glycol 300 and 5-8 parts by weight of sodium alginate are fully dissolved, 1-3 parts by weight of an antioxidant and 0.1 part by weight of carbetocin are added to the obtained system, the system is dissolved, the volume is fixed, the system is filtered, and the system is filled and sealed to obtain the carbetocin injection. The specific dose of glucose, polyethylene glycol 300 and sodium alginate is added as an auxiliary material, the three materials are matched with each other, the viscosity, density and intermolecular gap of the carbetocin in the injection are improved, so that the carbetocin in the carbetocin injection is prevented from gathering when the carbetocin injection is shaken, and the stability of the carbetocin injection is improved.