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Purification method of carbetocin

A carbetocin and purification method technology, which is applied in the field of carbetocin purification, can solve the problems of low product purity, single purification method, and low product yield, and achieve the effect of simple operation and obvious cost advantage

Inactive Publication Date: 2013-03-20
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Comprehensive analysis of the purification methods for the synthesis of carbetocin at home and abroad, there are two main problems: ①Using a single purification method, the product purity is low; ②Using multiple purification methods, the product yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Pretreatment: prepare carbetocin to be purified into water with a concentration of 200-300mg / ml

[0021] The solution was filtered through a microporous membrane with a pore size of 0.45um, and the filtrate was collected.

[0022] Reversed-phase column pretreatment: wash the column with 60% acetonitrile aqueous solution for 6 minutes, and then wash with 5% acetonitrile aqueous solution for 6 minutes.

[0023] Ion-exchange chromatography column pretreatment: Regenerate with NaOH aqueous solution with a concentration of 2mol / L for 6 minutes, then wash with pure water until neutral.

[0024] 2. Separation and purification: Take the filtrate, inject it into the treated chromatographic column for separation and purification, and elute the target

[0025] The peak of the liquid is divided into three sections: front, qualified product, and post-peak. The purity of the pre-peak part is 90%-99.5%, the purity of the qualified product part is greater than 99.5%, and the purity...

Embodiment 2

[0030] 1. Pretreatment: prepare carbetocin to be purified into water with a concentration of 200-300mg / ml

[0031] The solution was filtered through a microporous membrane with a pore size of 0.45um, and the filtrate was collected.

[0032] Reversed-phase column pretreatment: wash the column with 60% acetonitrile aqueous solution for 6 minutes, and then wash with 5% acetonitrile aqueous solution for 6 minutes.

[0033] Ion-exchange chromatography column pretreatment: Regenerate with NaOH aqueous solution with a concentration of 2mol / L for 6 minutes, then wash with pure water until neutral.

[0034] 2. Separation and purification: Take the filtrate, inject it into the treated chromatographic column for separation and purification, and elute the target

[0035] The peak of the liquid is divided into three sections: front, qualified product, and post-peak. The purity of the pre-peak part is 90%-99.5%, the purity of the qualified product part is greater than 99.5%, and the purity...

Embodiment 3

[0040] 1. Pretreatment: prepare carbetocin to be purified into water with a concentration of 200-300mg / ml

[0041] The solution was filtered through a microporous membrane with a pore size of 0.45um, and the filtrate was collected.

[0042] Reversed-phase column pretreatment: wash the column with 60% acetonitrile aqueous solution for 6 minutes, and then wash with 5% acetonitrile aqueous solution for 6 minutes.

[0043] Ion-exchange chromatography column pretreatment: Regenerate with NaOH aqueous solution with a concentration of 2mol / L for 6 minutes, then wash with pure water until neutral.

[0044] 2. Separation and purification: Take the filtrate, inject it into the treated chromatographic column for separation and purification, and elute the target

[0045] The peak of the liquid is divided into three sections: front, qualified product, and post-peak. The purity of the pre-peak part is 90%-99.5%, the purity of the qualified product part is greater than 99.5%, and the purity o...

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PUM

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Abstract

The invention discloses a purification method of carbetocin. The method comprises the following steps in sequence: purifying and synthesizing rough titanium through reversed-phase ion-pair chromatography, and removing an ion-pair agent through ion exchange resin, so as to obtain fine carbetocin peptide with purity of 99.5%. According to the purification method of carbetocin, the reversed-phase ion-pair chromatography is adopted, so that the purification yield and the purity of the carbetocin can be greatly improved.

Description

Technical field [0001] The present invention involves the field of purification technology of polypeptides, especially the purification technology of Kabebinyin. [0002] Background technique [0003] Carbetocin is a synthetic long -acting oxytic ninth peptide like a synthetic agent, which has no effect on the uterus of non -pregnancy, but has effective uterine contraction for uterus and newly produced uterus of pregnancy.The function, its clinical and pharmacological characteristics is similar to the catalysis produced by naturally.Like oxytocin, the combination of oxytocin receptors of cadroin and uterine smooth muscles causes the rhythmic contraction of the uterus. On the basis of the original contraction, it increases its frequency and increases the uterine tension.In the state of non -pregnancy, the oxytocin receptor content of the uterus is very low, which increases during pregnancy and peaks during childbirth.Endorgrome or lumbar anesthesia can be immediately administered ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/16C07K1/20C07K1/18
Inventor 陈阿娜
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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