77results about How to "High peptide yield" patented technology

The invention discloses a preparation method of solid phase peptide synthesis triptorelin, which includes the following steps: with Rink Amide AM resins or Rink Amide MBHA resins as starting materials, amino acids with protective groups are sequentially connected according to solid phase synthesis, so as to obtain protective decapeptide resins, and meanwhile crude products are obtained by sequentially removing Fmoc-protective groups and synchronously removing side-chain protective groups and cutting peptides, and triptorelin elaborate products are prepared after the crude products are separated and purified by C18 (or C8 ) column and freeze-dried. The preparation method is stable in technology, convenient in raw and auxiliary material sources, short in production cycle, high in yield, stable in quality, low in production cost and high in transpeptidase yield. Besides, as the preparation method avoids using poisonous reagents, such as hydrogen fluoride, and the like, the pollution of three wastes is low, purification yield is over 25 percent and each step of transpeptidase yield is above 98 percent; the yield after cutting peptides is 78.8 percent and the total yield is 25.4 percent.

The invention discloses a unique extracting method of maize albumen powder from maize, which comprises the following steps: using composite proteinase to enzymolyze zein; separating; hyperfiltering; condensing; spraying; drying; obtaining the white powder shaped product. The invention simplifies the technique and shortens the manufacturing period with good taste and small molecular weight of hybrid peptide, which can be clinical nutrition, hygienic food, sports food and cosmetics.

The invention discloses a method for preparing oxytocin through solid phase polypeptide synthesis, comprising following steps: taking Rink Amide resin (comprising Rink Amide MBHA resin, Rink Amide Am resin) as raw material, taking amino acid protected by Fmoc, TBTU or HBTU/ HOBt as condensing agent, making up amino acid in sequence; adding peptide cutting agent for peptide cutting, adding for precipitation and getting reduced coarse product; adding basic matter, feeding air for oxidation or oxiding with H2O2 with pH being 7.5- 10.0, getting oxidized coarse product; separating and purifying by using C18 or C 8 column and getting final product. The method is characterized by low production cost, simple process, little pollution, high production rate and convenience for industrial production.

The invention discloses a chalone preparing method of solid-phase polypeptide, which comprises the following steps: adopting 2-chloride-trityl resin, 4-methyl trityl resin or 4-methoxyl trityl resin as raw material; connecting amino acid with protective group according to solid-phase synthetic method; obtaining protected octapeptide resin; removing Fmoc-protective group sequently; stripping side-chain protective group; cutting peptide to obtain reduced aoqu-peptide; oxidizing through air under pH 7-11 condition; separating and purifying rought product through C18 (C8) column to produce exquisite.

The preparation process of solid phase polypeptide synthesized eptifibatide includes the following steps: (1) connecting amino acids one by one with Rink Amide resin, Rink Amide MBHA resin or Rink Amide AM resin as initial material, and Fmoc protected amino aids as monomer, with the last peptide chain being S-benzyl mercapto propionic acid Map(SBzl); (2) adding peptide cutting agent (TFA/HBr/HAc/TIS/EDT) to cut peptide; (3) precipitating and collecting coarse reductant eptifibatide product in ether solvent; (4) dissolving coarse reductant eptifibatide product in water, regulating pH value with ammonia water to 7.5-10.0, introducing air for oxidation, and collecting coarse eptifibatide product; and (5) separating and purifying the coarse product in C18 column to obtain the target product. The present invention has high yield, and is suitable for industrial production.

The invention provides a preparation method of sunflower seed polypeptide. The method comprises the following steps of: dissolving sunflower seed proteins isolate in water; hydrolyzing the sunflower seed proteins isolate with two-stage protease: alkaline protease and composite acid protease, to obtain the polypeptide, wherein during the hydrolysis, ultrasonic intermittent frequency conversion is performed to assist enzymolysis; and performing microfiltration and ultrafiltration on the enzymolysis liquid to obtain the sunflower seed polypeptide. The method has the advantages of adopting different ultrasonic frequencies and processing times for different proteases, effectively improving the hydrolysis degree and polypeptide yield of the sunflower seed proteins and obviously improving the production efficiency compared with the conventional process. A product prepared by the method has multiple health care functions of eliminating free radicals, removing nitrites, blocking the synthesis of nitrosamines, inhibiting the oxidization of red blood cells, beautifying and caring skins, preventing hepatic injury and the like and can be used for preparing functional foods and cosmetics.

The invention discloses a preparation method of solid phase peptide synthesis atosiban which includes the following steps: taking Rink Amide resins, Rink Amide MBHA resins or Rink Amide AM resins as starting materials and taking Fmoc amino acids as monomers, amino acids are grafted one by one and mercaptopropionic acids (Map(SX)) are protected by the last peptide chain; after protected nonapeptide resins are obtained, the acellular side-chain protective groups and cutting peptides are synchronized; then cutting peptides is carried out, and reduced crude atosiban is collected; the pH value is adjusted to 7.5 to 10.0, and oxidized crude atosiban is collected; target products are obtained by the separation and purification by preparative HPLC(C18 or C8 column). The preparation method is convenient in material source, simplifies technology and reduces cost; and the preparation method is low in the pollution of three wastes and is high in yield, and the preparation method is convenient for being industrialized and has good industrialization prospect.

The invention relates to a polypeptide synthesis method for octreotide acetate. The method comprises the following steps of: taking chloromethyl resin as a starting raw material, preparing a cesium salt from Boc-Thr(tBu)-OH, sequentially connecting amino acids with protecting groups according to a solid-phase synthesis method so as to obtain protected octapeptide resin, meanwhile, removing Boc protecting groups by sequentially using HCl/isopropyl alcohol, carrying out peptide connecting reaction in a manner of taking DIC and HOBT as condensing agents, carrying out reduction by using palladium carbon/hydrogen gas, meanwhile, cutting off peptide chains so as to obtain reduced octreotide, introducing air at the Ph of 7.8-9 so as to cyclize disulfide linkages, then, obtaining a crude octreotide product, and carrying out separation and purification through a C18 column, thereby preparing a fine octreotide acetate product. The method disclosed by the invention has the advantages that threoninol and Fmoc-threoninol are not adopted, the production cost is very low, the method has large-scale production capacity, the process is stable, the raw and auxiliary materials are convenient to obtain, the production cycle is short, the yield of connected peptide is high, the quality is stable, the use of highly-toxic reagents, such as hydrogen fluoride, trifluoroacetic acid and the like, is avoided, and the pollution caused by waste gas, waste water and waste residues is little.

The invention discloses a unique extracting method of egg albumin from eggs, which comprises the following steps: using composite proteinase to enzymolyze egg; separating; hyperfiltering; condensing; spraying; drying; obtaining the white powder shaped product. The invention simplifies the technique and shortens the manufacturing period with good taste and small molecular weight of hybrid peptide, which can be clinical nutrition, hygienic food, sports food and cosmetics.

The invention discloses a new salmon calcimar preparing method of Fmoc-strategy solid-phase polypeptide, which comprises the following steps: a. adopting Fmoc-Rink Amide MBHA or Rink Amide AM resin to connect the 32-piptide resin protected by kinds of amino acid after eluting Fmoc-protection sequently; b. eluting Fmoc-protective group sequently; c. removing side-chain protective group and cutting peptide synchronously to obtain reduced crude product; d. making crude product to do oxidation reaction through (7.5-10.0 pH) air; proceeding inverse-phase HPLC separation and purifying to produce fine salmon calcimar. The invention possesses scale producing capacity, which improves the quality, obtaining rate, and total obtaining rate.

The invention discloses an extraction method of tuna polypeptide and aims to mainly solve the problem that tuna polypeptide extracted by an existing method has fishy smell. The method includes: extracting protein in tuna processing leftovers via a thin salt solution, removing the fishy smell from the extracted tuna protein via modified bentonite, adjusting pH of tuna protein liquid before fishy smell removal according to the principle isoelectric precipitation of protein, precipitating the extracted tuna protein, thereby avoiding adsorption of the modified bentonite for the protein, enzymatically hydrolyzing the protein via neutral protease and compound protease to obtain the tuna polypeptide. The tuna polypeptide extracted by the method has no fishy smell and is high in yield.

The invention relates to a bioactivator preparation method, in particular to a placenta polypeptide preparation method. The placenta polypeptide preparation method comprises the steps of cutting a placenta into pieces, and adding normal saline into the cut placenta for tissue homogenate; mixing the obtained product with protease for enzymatic hydrolysis, and performing centrifugation and collecting centrifugate; performing extraction through reverse micelles to produce placental polypeptides and crude protease extract, subjecting the crude protease extract to ultrafiltration, collecting ultrafiltrate, and drying the ultrafiltrate to produce the placenta polypeptides. The preparation method is simple, production cost is low, equipment investment is little, and large-scale production is facilitated.

The invention discloses a new technology to prepare calf thymus alpha 1 (natural existing in thymus cells of calf and other animals), which uses Fmoc-strategy solid phase method, including the following steps: a. taking any one of Rink Amide PEGA resin, Rink Amide AM resin, Rink Amide MBHA resin or Rink Amide PEGA as the starting material, using the amino acid protected by Fmoc as the monomer to hang resin, connecting amino acids in turn to get a protective 28-peptide resin according to the method of solid-phase synthesis, b. using acetic anhydride to close heads during the process in turn, c. removing Fmoc-protective group in turn, d. synchronizing the removal of side-chain protecting group and the cut of peptide to get a crude, e. purifying the crude with antiphase HPLC to get thymosin alpha 1.

The invention discloses a Telis-vasophysin preparing method of solid-phase polypeptide, which comprises the following steps: adopting Rink Amide resin (concluding Rink Amide MBHA resin, Rink AmideAM resin) as original material, Fmoc protective amino acid as monomer, TBTU or HBTU-to-HOBt as condensing agent to connect amino sequently; using Boc-Gly-OH for the last peptide chain; adding peptide cutting agent to cut peptide; adding ether to deposit to obtain crude product; adding alkali material to oxidate at 7.5-10.0 pH value to generate oxide crude product; proceeding separation and purifying through C18 (or C8) pillar to produce object product. The method possesses low manufacturing cost, simple technology, high obtaining rate and low environmental pollution, which is convenient to do industrial construction.

The invention provides a technology for preparing capsule of liver cell growth promoting factor which comprises the steps of, extracting polypeptide dried powder from hepatocyte growth-promoting factors solution, charing diluent, mixing and palletizing, and loading into capsules, wherein the polypeptide dried powder extracting procedure comprises employing alcohol depositing method, charging ethanol into the solution of hepatocyte growth-promoting factors, refrigerating, separating, and collecting precipitation.

The invention relates to an octreotide acetate injection drug composition and application thereof. The octreotide acetate injection drug composition comprises 0.1g of octreotide acetate (by octreotide), 40-50g of mannitol, 3-4g of lactic acid, an appropriate amount of sodium hydrogen carbonate with the pH adjusted to 3.7-4.7, 1000ml of water for injection, and the like. The invention also discloses an octreotide acetate product and a preparation method thereof. The invention further relates to a method of determining impurities, such as oxidatively degraded impurities, in the octreotide acetate or the composition thereof. The octreotide acetate injection drug composition displays excellent technical effects as described in the description, for example, bulk drugs have higher production efficiency in the preparation process than those in the prior art, the drug loss during injection preparation is significantly reduced, and the growth rate of the impurities in long-term stability reserved samples is significantly lower than that in the prior art.