67 results about "Aceric acid" patented technology

The invention relates to the technical field of biological detection, and discloses a mycoplasma pneumoniae nucleic acid rapid detection method, which comprises the following steps: mixing a sample to be detected with a multifunctional pretreatment buffer solution containing tris (hydroxymethyl) aminomethane, potassium acetate, polyethylene glycol, saponin, spermine tetrahydrochloride and tris (2-carboxyethyl) phosphine hydrochloride, and carrying out cracking treatment; a cracking mixed solution is obtained; redissolving the enzymatic isothermal amplification basic reagent, the specific primer and the probe to obtain a redissolving amplification system; sucking the supernatant of the cracking mixed solution, adding the supernatant into a redissolving amplification system, and adding a magnesium acetate solution to obtain a reaction mixed solution to be detected; and carrying out isothermal amplification and signal acquisition to obtain a detection conclusion. The multifunctional pretreatment buffer solution is used for cracking the to-be-detected sample, sample liquefaction, cracking and nucleic acid release are completed in a single system, the nucleic acid extraction and purification step of centrifugal column adsorption or magnetic bead elution is avoided, and the operation process is simplified.

The invention discloses a method for extracting and preparing angelica keiskei exosomes. The method comprises the following steps: S1, selecting an angelica keiskei sample and selecting chitosan; s2, crushing and filtering the angelica keiskei sample to obtain an extracting solution; s3, dissolving chitosan through acetic acid, forming a film through a rotary coater, and carrying out drying and heat treatment; s4, mixing the extracting solution with the dried chitosan membrane, and adsorbing the exosome through the charge effect; s5, putting the chitosan membrane into a tangential flow filtration system, collecting effluent after tangential flow filtration, and purifying the effluent; and S6, pre-freezing the purified solution, and carrying out drying treatment. The invention also discloses application of the method. The method has the beneficial effects that the angelica keiskei sample is crushed and filtered to obtain the extracting solution, then the extracting solution and the dried chitosan film are mixed at room temperature, the exosome is adsorbed through the charge effect, and the exosome is purified after being filtered by the tangential flow filtering system, so that a large amount of high-purity exosome is obtained, and the method is simple, convenient and efficient.

The invention relates to the technical field of chemical engineering, and discloses a method for improving the chromaticity and purity of acetic anhydride based on a reflux ratio, and the method comprises the following steps: optimizing an evaporation tower, an acetic acid rectifying tower and an acetic anhydride rectifying tower, and installing an online detection instrument and an automatic control valve to construct an intelligent rectification control system; evaporating the crude acetic anhydride by using the optimized evaporation tower to obtain crude acetic anhydride steam; feeding the crude acetic anhydride steam into an acetic acid rectifying tower, calculating an initial reflux ratio, inputting the initial reflux ratio into a control system, and starting a reflux system to preliminarily remove acetic acid; monitoring the acetic acid content through an online gas chromatograph, and finely adjusting the reflux ratio until the reflux ratio reaches the standard when the measured acetic acid content is higher than a target value; feeding the crude acetic anhydride reaching the standard into an acetic anhydride rectifying tower, calculating an initial reflux ratio, inputting the initial reflux ratio into a control system, and starting to obtain an intermediate product; monitoring chromaticity and purity through an on-line platinum-cobalt colorimeter and an on-line gas chromatograph, and updating the reflux ratio to be qualified according to a bivariate adjustment formula; and finally, carrying out all-item detection on a finished product, and locking reflux ratio parameters after confirming that the finished product is qualified.

The utility model provides a solid acetic acid homogenizing fermentation drum which comprises a fermentation drum body, a feed port, a liquid inlet, a stainless steel pipe, a flange, a heating belt, an electric slip ring, a rack, a motor transmission system and a transmission shaft. Raw materials are conveyed through the left-side pipeline, wine and acetic bacteria are conveyed through the right-side pipeline, and mechanical conveying of the raw materials is achieved; a temperature control device is arranged, so that the temperature of vinegar grains in the fermentation drum can be monitored in real time to determine a fire point; a heating belt is wound on the drum body, and the drum body is wound through an electric slip ring and a heating belt connector, so that the entangling phenomenon can be avoided when the fermentation drum rotates forwards and backwards, and materials in the drum can be heated to reach a proper fermentation temperature; a rack is arranged below the rotary drum, transmission shafts are arranged on the periphery of the rack, and the fermentation rotary drum is positively and negatively rotated through rollers, so that the fermentation uniformity of vinegar grains in the rotary drum is ensured. According to the utility model, the problems of more required equipment and manpower and unstable acetic fermentation in the acetic fermentation stage are solved.

The utility model relates to the technical field of chemical separation, in particular to a dual-purpose extractive distillation tower for separating acetic acid from water, which comprises an extraction tower, a condenser I, a condenser II and a reboiler, an extraction chamber, a distillation chamber and a circulation chamber are arranged in the extraction tower, the distillation chamber and the extraction chamber are symmetrically arranged, and the circulation chamber is positioned below the distillation chamber and the extraction chamber. The first condenser and the second condenser are located above the extraction tower, the first condenser is communicated with the extraction chamber, the second condenser is communicated with the rectification chamber, and by integrating the two functions of extraction and rectification, the tower achieves effective separation of acetic acid and water. The layered contact design ensures that the acetic acid raw material and the composite extraction agent are in full contact but not directly mixed between filler layers, the mass and heat transfer process is optimized, the extraction agent can be recycled after being treated by the rectification chamber, and the resource waste is remarkably reduced. And due to the recycling design, the operation cost is reduced, and the consumption of the extracting agent is reduced.

The invention provides high-activity crataegus pinnatifida fruit vinegar and a preparation method thereof.The preparation method comprises the steps that crataegus pinnatifida fruits are juiced, then a composite chelating agent composed of polyglucuronic acid and monopotassium phosphate is added for chelating treatment, and a polysaccharide-metal ion cross-linked network is removed; then adding endo-beta-1, 4-glucanase to carry out linear polysaccharide directional enzymolysis, so that the viscosity of the fruit juice is obviously reduced; after the components are adjusted, double modified yeast beta-glucan microspheres are added, yeast is inoculated for alcoholic fermentation, the microspheres are subjected to double grafting modification through galactooligosaccharide and theaflavin, and active components in the crabapple can be directionally captured and stabilized through hydrophilic and hydrophobic effects respectively; after alcohol fermentation is finished, acetic bacteria are inoculated for acetic fermentation, and finally, filtering and sterilization are performed. According to the method disclosed by the invention, by virtue of a synergistic strategy of breaking a network firstly, then carrying out enzymolysis debonding and then carrying out microsphere loading, the fermentation mass transfer efficiency is effectively improved, and meanwhile, the retention rate of functional components such as polyphenol and saponin in the fruit vinegar is increased.

This application provides a method for preparing pH-responsive polyamino acid drug-loaded nanoparticles based on sarcosine and their application. Sar-NNCA, L-lysine-NCA, and L-phenylalanine-NCA are dissolved in anhydrous DMF and polymerized under argon protection with a hexamethyldisilazine initiator to obtain a polyamino acid block copolymer Sar-NNCA. 80 -Lys(Cbz) n -Phe 10 The crude product was deprotected under HBr / acetic acid and loaded with DOX to obtain Sar. 80 -Lys n -Phe 10 -DOX. This application describes the preparation of a pH-responsive polymer nanoplatform, Sar, by encapsulating doxorubicin in polysarcosine-polylysine-polyphenylalanine nanoparticles via Schiff base bonds. 80 -Lys n -Phe 10 -DOX is used for tumor treatment. These polyamino acid nanoparticles are spherical with an average particle size of approximately 200 nanometers, exhibiting pH-responsive properties, excellent cellular uptake capacity, and anti-tumor effects. In vitro experiments show that Sar... 80 -Lys n -Phe 10 The carrier material exhibits excellent biocompatibility. The newly synthesized Sar... 80 -Lys n -Phe 10 -DOX nanomicelles show promise as a drug delivery system for cancer treatment.

The invention discloses a peach ketone synthesis method, which relates to the technical field of essence and perfume synthesis, and comprises the following steps: adding cyclopentanone and glacial acetic acid into a reaction container, starting stirring, adding manganese acetate (II) and cobalt acetate (II), and then adding acetic anhydride; introducing dried air below the liquid level, heating to 80-100 DEG C, dropwise adding D-limonene, and carrying out a heat preservation reaction after dropwise adding is finished; gC monitors the reaction endpoint, and the reaction is stopped when the product purity of the reaction liquid is not increased any more; after the reaction is finished, filtering, carrying out reduced pressure distillation to recover cyclopentanone, glacial acetic acid and unreacted D-limonene, after the recovery is finished, adding methanol and n-heptane into kettle liquid, carrying out water extraction, washing and liquid separation, washing an organic phase with a sodium bicarbonate aqueous solution, carrying out reduced pressure distillation on the organic phase to recover n-heptane, and after the recovery is finished, obtaining a peach ketone crude product; and carrying out crude drawing and rectification on the peach ketone crude product to obtain a peach ketone finished product. The method has the advantages of mild reaction conditions, low safety risk and few three wastes, and can obtain a high-purity peach ketone finished product.

The invention discloses a method for producing volatile fatty acid by improving microbial electro-catalytic conversion of CO2. The core of the method is to construct an efficient and stable heterozygote biological cathode. The method comprises the following steps: inoculating flora mainly comprising Acetobacter burgii and Desulfurization Vibrio in a cathode chamber of an H-type microbial electrosynthesis reactor, and adding a zinc source and a sulfur source to synthesize ZnS nanoparticles in situ on the surfaces of cells, so as to construct the bacterium and ZnS nanoparticle heterozygote biological cathode. The acetic acid yield of the heterozygote biological cathode can reach 3.38 mmol / L / d, and the accumulative yield of one batch is 20.25 mmol / L, which is 2.7 times that of an unmodified carbon cloth biological cathode; the coulombic efficiency of the total volatile fatty acid product reaches 96.47% and is 1.25 times that of a carbon cloth biological cathode; and the coulombic efficiency of acetic acid and the selectivity of acetic acid are respectively improved to 83.27% and 86.32%, which are obviously higher than 40.63% and 52.64% of those of a carbon cloth biological cathode. In addition, the charge transfer resistance of the heterozygote biological cathode is as low as 3.75 ohm and is obviously superior to that of a carbon cloth biological cathode (25.28 ohm), so that long-term stable and high-selectivity acetic acid production is realized.

The invention discloses a magnetic amide functionalized covalent organic framework material and a preparation method and application thereof, the preparation method comprises the following steps: S1, uniformly dispersing Fe3O4 nanoparticles and ammonia water, adding tetraethyl orthosilicate, and reacting; s2, a product obtained in the step S1 and 3-aminopropyltriethoxysilane are uniformly dispersed and subjected to a reaction, and aminated magnetic nanoparticles Fe3O4 (at) SiO2-NH2 are obtained; and S3, uniformly dispersing the Fe3O4 (at) SiO2-NH2 and trialdehyde phloroglucinol in a mixed solution of o-dichlorobenzene and N, N-dimethylformamide, adding 4, 4 '-diaminobenzanilide, uniformly mixing, then adding an acetic acid solution, and reacting at the temperature of 100-120 DEG C for 3-5 days to obtain the magnetic covalent organic framework Fe3O4 (at) Am-COFs material. The Fe3O4atAm-COFs prepared by the preparation method disclosed by the invention has a large specific surface area, rapid magnetic separation capability and abundant active sites, so that the adsorption performance of the material on a target object is effectively improved.

The invention relates to the technical field of acetic acid rectification, and discloses a glacial acetic acid rectification device with a purification function, the device comprises a distillation tower, a T-shaped ring is arranged in the distillation tower, the distillation tower and the tower wall form an annular flow channel, a central shaft is rotatably arranged at the bottom of the distillation tower, a conical cover is arranged at the top end of the shaft, and a condensation mechanism is arranged between the central shaft and the conical cover; a spiral stirring blade is arranged at the middle lower section of the central shaft, and the bottom end of the central shaft is in transmission connection with each reciprocating shaft through a swinging mechanism; by integrating chemical reaction purification, global stirring and dynamic condensation technologies, the rectification efficiency and the product purity are remarkably improved, long-term stable operation of the system is guaranteed through the purification and anti-scaling design, and the comprehensive performance is superior to that of traditional equipment.

The invention discloses a nutrition activator for improving fermentation acid production activity of acetic bacteria and application of the nutrition activator, and belongs to the technical field of food fermentation. The nutrition activator disclosed by the invention is prepared from the following nutritional ingredients in percentage by concentration: 1.00 to 1.50 percent of acetic acid, 1.00 to 2.00 percent of yeast extract, 1.50 to 2.50 percent of trehalose and 0.10 to 0.20 percent of CaCl2. The nutrition activator can improve the fermentation acid production activity of acetic acid bacteria, is a natural and safe nutrition activator, can activate the acetic acid bacteria, improves the activity of ethanol dehydrogenase and acetaldehyde dehydrogenase of the acetic acid bacteria in vinegar fermentation, improves the acid resistance of the acetic acid bacteria, and improves the fermentation efficiency and the acetic acid yield.

The application discloses a high-temperature inducible promoter and application thereof, and belongs to the field of genetic engineering and fermentation engineering. Acetobacter pasteurianus A high-temperature inducible promoter P icd is screened from a bacillus pabuli (Olsenella ) and has a nucleotide sequence as shown in SEQ ID NO. 1. The application further constructs a recombinant expression vector pBBR1MCS2-icd containing the promoter and a recombinant bacterium expressing an ethanol dehydrogenase gene AdhP. The recombinant strain ApAdhP(icd) using the promoter to regulate the expression of the ethanol dehydrogenase can not only reduce the metabolic burden of the cell and maintain a good growth trend under high-temperature fermentation conditions, but also can reach an acetic acid final yield of 52.65 g / L, which is increased by about 31.5% compared with a wild strain. The application effectively solves the problems of the decrease of acetic acid production rate and yield under high-temperature environment in summer in traditional acetic acid fermentation.

The invention belongs to the technical field of genetic engineering, and discloses a genetically engineered bacterium for efficiently synthesizing gamma-aminobutyric acid from the beginning by efficiently utilizing a mixed carbon source, a method and application, and the genetically engineered bacterium is obtained by overexpressing edd from Zymomonas mobilis on the basis of escherichia coli E. coli G16; eda from the zymomonas mobilis is over-expressed; performing overexpression on acs (Acetobacter pasteurianus) sources; a malic acid synthase gene aceB is knocked out; a malic acid synthase gene glcB is knocked out; and the acetyl phosphate transferase gene eutD is knocked out. The escherichia coli strain which is clear in genetic background and capable of producing gamma-aminobutyric acid is utilized, and an ED path and an acetic acid utilization path are introduced, so that acetyl CoA branch metabolism is reduced, and the yield and conversion rate of gamma-aminobutyric acid are remarkably improved.

The application discloses a compound amino acid injection (15-HBC) composition and a preparation method thereof, and belongs to the technical field of medicines. The compound amino acid injection (15-HBC) composition provided by the application comprises the following components in an amount of 250mL of the composition: isoleucine 1.532-2.298g, leucine 2.756-4.134g, lysine acetate 1.160-1.740g, methionine 0.500-0.750g, phenylalanine 0.640-0.960g, threonine 0.400-0.600g, tryptophan 0.180-0.270g, valine 1.772-2.658g, alanine 0.800-1.200g, arginine 1.160-1.740g, histidine 0.320-0.480g, proline 1.260-1.890g, serine 0.660-0.990g, glycine 0.660-0.990g, cysteine hydrochloride 0.040-0.060g, and appropriate amount of glacial acetic acid for pH adjustment, and the rest is injection water. The application also provides a preparation method of the compound amino acid injection (15-HBC) without sulfite antioxidant. Meanwhile, the activated carbon adsorption process is removed in the preparation, the control of raw material packages, production processes and the like is strengthened to control bacterial endotoxins, the production process is simplified, and the product quality is ensured.

The invention belongs to the technical field of oil exploitation, and particularly relates to a composite blocking remover and a preparation method and application thereof. The composite blocking remover comprises the following components in percentage by weight, comprising the following components in percentage by weight: 5 to 8 percent of hydrochloric acid, 1 to 3 percent of fluoboric acid, 0.5 to 2 percent of acetic acid, 0.5 to 1 percent of dodecyl trimethyl ammonium chloride, 0.1 to 0.3 percent of potassium chloride, 0.3 to 0.8 percent of sodium erythorbate, 0.2 to 0.5 percent of tetrasodium glutamate diacetate, 1 to 2 percent of ethylenediamine tetraacetic acid, 0.1 to 0.2 percent of inositol hexaphosphate, 0.1 to 0.2 percent of cocoanut fatty acid diethanolamide, 0.3 to 0.5 percent of sodium dodecyl benzene sulfonate, 0.3 to 0.5 percent of dimethyl diallyl ammonium chloride, 0.1 to 0.2 percent of ethylene glycol monobutyl ether and the balance of water. The composite blocking remover provided by the invention does not generate the problem of secondary precipitation, slows down the reaction speed of acid rocks, effectively pushes a blocking removal measure solution into the deep part of a stratum, enlarges the blocking removal radius, realizes deep treatment of the stratum, improves the acidification effect, and prolongs the effective action time of acidification.

The utility model relates to the technical field of chemical separation, in particular to a liquid-liquid extraction tower for separating acetic acid from water, which comprises an extraction tower, an extraction agent feed port arranged at the bottom end of the extraction tower, a raw material feed port arranged at the top end of the extraction tower, an extraction phase discharge chute arranged at the top end of the extraction tower and an extraction phase discharge port arranged at the top end of the extraction tower. An upper U-shaped bent pipe is mounted on a discharge port of the extraction box, an extracted mixture forms an extraction phase at the top of the tower, and the extraction phase is collected through the extraction phase discharge groove and discharged through the extraction phase discharge port. And an upper U-shaped bent pipe is mounted on the extraction phase discharge port, so that the waste water is prevented from being carried to the extraction phase discharge port, the unextracted residual solution is prevented from being formed at the bottom of the tower, and the residual solution is collected through the raffinate phase discharge groove and then discharged through the raffinate phase discharge port. And a lower U-shaped bent pipe is arranged on the raffinate phase discharge hole, so that the extracting agent is prevented from overflowing from the tower kettle.

The invention discloses 1, 3-bifunctional isobutyramide pyridinium salt, a preparation method and application of the 1, 3-bifunctional isobutyramide pyridinium salt in antiviral drugs, and belongs to the field of drug synthesis. The preparation method comprises the following steps: under the protection of argon, by taking (3-(2-fluorophenyl)-2, 2-dimethyl propionyl) (pyridine-2-yl) amide as a raw material and dichloroethane as a solvent, adding palladium acetate, iodobenzene diacetate, water and acetic acid, and reacting to obtain 1, 3-hydroxyl / acetoxy pyridinium salt; the preparation method comprises the following steps: by taking N-(3-((2-(2-fluoro-4-phenylbiphenyl-3-yl)-2-methylpropionyl) oxy)-2, 2-dimethyl propionyl) pyridinium as a raw material and toluene as a solvent, adding palladium acetate, 4, 5-diazafluorene-9-one, iodobenzene diacetate, potassium carbonate and acetic anhydride, and reacting to obtain the 1, 3-diacetoxypyridinium salt. The synthesis process of the 1, 3-bifunctional isobutyramide pyridinium salt is simple and convenient, and the reaction conditions are mild; meanwhile, the pyridinium salt has good anti-enterovirus activity and is expected to become a medicine for effectively treating EV-A71 infection.

The invention discloses a cholate synergistic effect-based open wound medicine formula and a preparation method thereof, cholate and sodium chloride are subjected to high-pressure homogenization and grinding to nanoscale, acetic acid, water and alcohol are sequentially added, after pH is adjusted, terminal filtration is carried out through a 0.22-micron filter membrane, sterility is ensured, the formula can penetrate into a corium layer to inhibit inflammatory factors (such as TNF-alpha), and the open wound medicine has a good application prospect. And an epidermal cell migration signal (such as TGF-beta 1) is activated, so that the healing period is shortened to 8-10 days. The nano-scale dispersion improves the medicine permeation efficiency, the weak acid environment inhibits the formation of a gram-positive bacterium biofilm, and the eye drops are free of sensitization, are suitable for immunodeficiency patients and have auxiliary effects on haemorrhoids and eye diseases.

The invention belongs to the technical field of acetic acid analysis, and particularly relates to a quick-mounting acetic acid water content analyzer which comprises an adjusting plate, a near-infrared spectrum analyzer is fixedly mounted on one side of the adjusting plate, a spectrum absorption sensor is electrically connected to the near-infrared spectrum analyzer through a connecting wire, and the quick-mounting acetic acid water content analyzer comprises a quick clamping assembly; the rapid clamping assembly comprises two adjusting blocks slidably connected to one side of the adjusting plate, and pulleys are rotationally arranged on the two sides of each adjusting block. By means of the rapid clamping assembly, the acetic acid water content in multiple areas in the pipeline can be detected, and therefore the accuracy of an analysis result can be improved. In addition, compared with the mode that a set of sensors is installed at each detection point, the scheme has the advantage that the cost is remarkably reduced while the multi-point detection requirement is met.

The invention belongs to the technical field of traditional Chinese medicines, and discloses a Shenshen cream with the effects of nourishing blood and tranquilizing mind, improving sleep and resisting oxidative damage. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-16 parts of spina date seeds, 10-16 parts of red dates, 8-12 parts of longan aril, 8-12 parts of lotus seeds, 1-3 parts of radix angelicae sinensis, 5-10 parts of lucid ganoderma, 5-8 parts of radix astragali, 6-10 parts of chicory, 5-8 parts of lily bulbs and 3-5 parts of radix puerariae. According to the cream formula, the ganoderma lucidum and the angelica sinensis are fermented by adopting lactobacillus rhamnosus; and carrying out enzymolysis treatment on the astragalus membranaceus by using a compound enzyme. The ointment can significantly increase the number of red blood cells of a patient with blood deficiency; the sleep time is prolonged and the sleep quality is improved; the obvious oxidation resistance is realized. In addition, the ointment provided by the invention can improve the pain threshold value of mice in an acetic acid writhing method. Through unique raw material compatibility and process innovation, modern efficient utilization of traditional medicinal and edible materials is realized.

The present application belongs to the technical field of pharmaceutical analysis and quality control, and relates to a detection method for the content of a lobaplatin intermediate, in particular to a detection method for the content of trans-1,2-diaminocyclobutane diiodoplatinum (II), which is an HPLC method, and the detection conditions are as follows: octadecyl bonded silica is used as a filler, an ammonium acetate solution is used as a mobile phase A, and a mixed solution of the ammonium acetate solution and acetonitrile is used as a mobile phase B, and gradient elution is performed. The detection method has strong specificity, good linear relationship, high accuracy and high precision. The present application has important guiding significance for the quantity of material feeding in subsequent reactions, the improvement of the yield and purity of lobaplatin by establishing the detection method for the content of the lobaplatin intermediate trans-1,2-diaminocyclobutane diiodoplatinum.

The invention discloses a method for evaluating the analgesic effect of angelica sinensis stomachache dropping pills, belongs to the technical field of efficacy evaluation, and aims to solve the problem that no scientific method is provided for efficacy verification of dysmenorrhea traditional Chinese medicine preparations in the formula of angelica sinensis. The method comprises the steps of experimental mouse selection, acetic acid body twisting model test, hot plate pain model test and xylene inflammation model test. Selecting an experimental rat, and testing the primary dysmenorrhea model constructed by estradiol and oxytocin. The method comprises the following steps: constructing an analgesic effect evaluation model, and verifying analgesic and anti-inflammatory effects of angelica sinensis stomachache relieving; pharmacodynamic mechanism analysis is further carried out on the angelica sinensis stomachache dropping pill for simulating primary dysmenorrhea, it is revealed that the content of serum E2, IL-6, PROG, beta-EP and TNF-alpha of primary dysmenorrhea model rats treated by the angelica sinensis stomachache dropping pill has an adjusting effect, the effect of relieving dysmenorrhea can be achieved, the inflammation effect can be relieved by inhibiting NF-kappa B and MAPK channels, and the pain behavior can be relieved.

The invention discloses a podophyllotoxin-loaded nano-drug as well as a preparation method and application thereof. The nano-drug is of a core-shell structure, podophyllotoxin is entrapped by lecithin to form a hydrophobic core, and modified chitosan is coated on the surface to serve as a shell layer. The preparation method comprises the following steps: injecting an ethanol solution of lecithin and podophyllotoxin into an acetic acid aqueous solution of modified chitosan, incubating and centrifuging to obtain the modified chitosan, and the preparation process is simple and convenient. The water solubility and the stability of podophyllotoxin can be remarkably improved through the prepared nano-drug, the anti-tumor immune response of a body is activated while efficient chemotherapy is achieved through the immunologic adjuvant function of the modified chitosan, the synergistic treatment effect is generated, and the nano-drug has the excellent inhibition effect on solid tumors.

The utility model relates to the technical field of chemical separation, in particular to a novel composite extractant for refining acetic acid, which comprises an extraction tower, a recovery tower, a reboiler and a condenser, an extractant feed port is arranged at the top end of the extraction tower, a raw material inlet is arranged at the bottom end of the extraction tower, and a discharge pipe is arranged at the bottom of the extraction tower. The discharge pipe is communicated with the recovery tower through a recovery pipe, a liquid discharge pipe is arranged at the bottom of the recovery tower, a flow guide pipe I is arranged on the liquid discharge pipe, the reboiler comprises a reboiler I and a reboiler II, the reboiler I is communicated with the discharge pipe and the extraction tower through a circulating pipe I, and the extraction tower and the recovery tower are linked. Efficient separation and cyclic utilization of the extraction agent and acetic acid are achieved, waste of raw materials is reduced, the extraction agent can be effectively recycled and recycled through the recovery tower, consumption of chemicals and generation of waste are reduced, and the environmental protection benefit is remarkable.

The invention relates to a method for determining the lipid content in lipid nanoparticles based on RP-UPLC and CAD combination and application. The method comprises the following steps: detecting a lipid nanoparticle test solution and a standard solution by adopting a reversed-phase high performance liquid chromatography and electric fog detector combination method; the detection conditions of the reversed-phase high performance liquid chromatography are as follows: a phenyl column is adopted as a chromatographic column, triethylamine acetate-water is adopted as a mobile phase A, and a triethylamine acetate-acetonitrile solution is adopted as a mobile phase B for gradient elution. The analysis method is good in repeatability and wide in application range, not only can be used for measuring the lipid content in mRNA-LNP, but also is suitable for quantification of lipid in unwrapped nucleic acid drugs, and has important significance for guiding process research and development and formula identification of lipid nanoparticles in the production process.

The application discloses a drug sustained-release elastic embolism microsphere and a preparation method and application thereof, and belongs to the technical field of medical materials. The application is prepared by the following steps: taking an acetic acid aqueous solution in which chitosan is dissolved as a dispersed phase, mixing the dispersed phase and a continuous phase into a W / O monodisperse emulsion template through microfluidic technology, introducing the W / O monodisperse emulsion template into an acceptant phase containing genipin, a W / O surfactant and an organic oil, crosslinking under magnetic stirring for 4-24 hours, post-treating the obtained microspheres to obtain elastic embolism microspheres, and finally dispersing the elastic embolism microspheres in an impregnation liquid in which a hydrophilic antitumor drug is dissolved and performing oscillation treatment, so that the drug sustained-release elastic embolism microsphere is prepared. The application effectively optimizes the drug loading capacity and drug sustained-release performance of the formed microspheres by setting the single chitosan dispersed phase component and adopting the technical strategy of jointly regulating and controlling the microfluidic control emulsification and crosslinking time, so that the effect of simply preparing the drug sustained-release elastic embolism microsphere is achieved.

According to the fermented beverage rich in alpha-ketoglutaric acid and organic acid and the preparation method of the fermented beverage, hawthorn fruits are used for preparing 3.5-degree culture wine, the culture wine is rich in amino nitrogen, and rich protein is provided for enrichment of alpha-ketoglutaric acid and organic acid generated through fermentation; acetic acid bacteria are cultured in the culture wine and ignited, mature fermented wine is obtained when the acidity is larger than 3 degrees, the acetic acid bacteria grow into a tricarboxylic acid state when fermentation is vigorous, the acetic acid bacteria are continuously split and grow, and alpha-ketoglutaric acid reaches the enrichment concentration in acetic acid bacteria cells; the method comprises the following steps of: preparing a 3-degree acid stock solution, boiling, evaporating and concentrating the 3-degree acid stock solution to 50% at 60 DEG C through vacuum steam concentration, removing excessive moisture and acidity according to the ratio of naturally proportioned acidity to AKG through concentration evaporation, amplifying the concentration ratio of AKG, and selecting proper concentration acidity to maximize the concentration ratio of AKG when the proper acidity is obtained through blending, so that the concentration ratio of AKG is increased, the concentration ratio of AKG is increased, and the concentration ratio of AKG is increased. Finally, the acidity of the fermented beverage is 0.9-1 degree through blending, and the concentration of the alpha-ketoglutaric acid is 26 mg / L.