109 results about "Aceric acid" patented technology

The invention discloses a preparation method of a cell inflammatory factor adsorbent, which comprises the following steps: mixing styrene, divinyl benzene and hydroxyethyl acrylate, and adding a pore-foaming agent and mesoporous SiO2 nano powder to prepare a porous microsphere carrier; the preparation method comprises the following steps: mixing chitosan and poly (N-isopropylacrylamide) in an acetic acid solution to obtain a temperature-sensitive chitosan hydrogel precursor solution; the preparation method comprises the following steps: activating the surface of a porous microsphere carrier by using 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, mixing the activated porous microsphere carrier and a temperature-sensitive chitosan hydrogel precursor solution to obtain a water phase, and assembling, washing and drying in an oil phase to obtain the cell inflammatory factor adsorbent. Through the design of the core-shell structure and the addition of the mesoporous SiO2 nano powder, the temperature response characteristic and the recyclable characteristic are given to the cell inflammatory factor adsorbent, the adsorption capacity of cell factors is also improved, and a new solution is provided for physical adsorption of the cell inflammatory factors.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention relates to the technical field of biological detection, and discloses a mycoplasma pneumoniae nucleic acid rapid detection method, which comprises the following steps: mixing a sample to be detected with a multifunctional pretreatment buffer solution containing tris (hydroxymethyl) aminomethane, potassium acetate, polyethylene glycol, saponin, spermine tetrahydrochloride and tris (2-carboxyethyl) phosphine hydrochloride, and carrying out cracking treatment; a cracking mixed solution is obtained; redissolving the enzymatic isothermal amplification basic reagent, the specific primer and the probe to obtain a redissolving amplification system; sucking the supernatant of the cracking mixed solution, adding the supernatant into a redissolving amplification system, and adding a magnesium acetate solution to obtain a reaction mixed solution to be detected; and carrying out isothermal amplification and signal acquisition to obtain a detection conclusion. The multifunctional pretreatment buffer solution is used for cracking the to-be-detected sample, sample liquefaction, cracking and nucleic acid release are completed in a single system, the nucleic acid extraction and purification step of centrifugal column adsorption or magnetic bead elution is avoided, and the operation process is simplified.

The invention discloses a method for extracting and preparing angelica keiskei exosomes. The method comprises the following steps: S1, selecting an angelica keiskei sample and selecting chitosan; s2, crushing and filtering the angelica keiskei sample to obtain an extracting solution; s3, dissolving chitosan through acetic acid, forming a film through a rotary coater, and carrying out drying and heat treatment; s4, mixing the extracting solution with the dried chitosan membrane, and adsorbing the exosome through the charge effect; s5, putting the chitosan membrane into a tangential flow filtration system, collecting effluent after tangential flow filtration, and purifying the effluent; and S6, pre-freezing the purified solution, and carrying out drying treatment. The invention also discloses application of the method. The method has the beneficial effects that the angelica keiskei sample is crushed and filtered to obtain the extracting solution, then the extracting solution and the dried chitosan film are mixed at room temperature, the exosome is adsorbed through the charge effect, and the exosome is purified after being filtered by the tangential flow filtering system, so that a large amount of high-purity exosome is obtained, and the method is simple, convenient and efficient.

The invention relates to the technical field of chemical engineering, and discloses a method for improving the chromaticity and purity of acetic anhydride based on a reflux ratio, and the method comprises the following steps: optimizing an evaporation tower, an acetic acid rectifying tower and an acetic anhydride rectifying tower, and installing an online detection instrument and an automatic control valve to construct an intelligent rectification control system; evaporating the crude acetic anhydride by using the optimized evaporation tower to obtain crude acetic anhydride steam; feeding the crude acetic anhydride steam into an acetic acid rectifying tower, calculating an initial reflux ratio, inputting the initial reflux ratio into a control system, and starting a reflux system to preliminarily remove acetic acid; monitoring the acetic acid content through an online gas chromatograph, and finely adjusting the reflux ratio until the reflux ratio reaches the standard when the measured acetic acid content is higher than a target value; feeding the crude acetic anhydride reaching the standard into an acetic anhydride rectifying tower, calculating an initial reflux ratio, inputting the initial reflux ratio into a control system, and starting to obtain an intermediate product; monitoring chromaticity and purity through an on-line platinum-cobalt colorimeter and an on-line gas chromatograph, and updating the reflux ratio to be qualified according to a bivariate adjustment formula; and finally, carrying out all-item detection on a finished product, and locking reflux ratio parameters after confirming that the finished product is qualified.

The utility model provides a solid acetic acid homogenizing fermentation drum which comprises a fermentation drum body, a feed port, a liquid inlet, a stainless steel pipe, a flange, a heating belt, an electric slip ring, a rack, a motor transmission system and a transmission shaft. Raw materials are conveyed through the left-side pipeline, wine and acetic bacteria are conveyed through the right-side pipeline, and mechanical conveying of the raw materials is achieved; a temperature control device is arranged, so that the temperature of vinegar grains in the fermentation drum can be monitored in real time to determine a fire point; a heating belt is wound on the drum body, and the drum body is wound through an electric slip ring and a heating belt connector, so that the entangling phenomenon can be avoided when the fermentation drum rotates forwards and backwards, and materials in the drum can be heated to reach a proper fermentation temperature; a rack is arranged below the rotary drum, transmission shafts are arranged on the periphery of the rack, and the fermentation rotary drum is positively and negatively rotated through rollers, so that the fermentation uniformity of vinegar grains in the rotary drum is ensured. According to the utility model, the problems of more required equipment and manpower and unstable acetic fermentation in the acetic fermentation stage are solved.

The invention relates to a pharmaceutical intermediate (S)-9-(2, 3-di-O-acetyl propyl)-N2-acetyl guanine and a synthesis method thereof.In the synthesis method, a compound 2 serves as a starting material, sodium acetate, acetic acid and acetic anhydride are added, reaction, concentration and column chromatography purification are performed at the temperature of 110-130 DEG C, and the target product (S)-9-(2, 3-di-O-acetyl propyl)-N2-acetyl guanine is obtained. The invention relates to 2, 3-di-O-acetyl propyl)-N2-acetyl guanine. The invention discloses a novel medical intermediate and designs a feasible synthesis process, through the synergistic effect of all reagents, the reaction can be stabilized under mild conditions, side reactions are effectively avoided, the purity and stability of the product are ensured, and a foundation is laid for popularization of new drugs.

The utility model relates to the technical field of chemical separation, in particular to a dual-purpose extractive distillation tower for separating acetic acid from water, which comprises an extraction tower, a condenser I, a condenser II and a reboiler, an extraction chamber, a distillation chamber and a circulation chamber are arranged in the extraction tower, the distillation chamber and the extraction chamber are symmetrically arranged, and the circulation chamber is positioned below the distillation chamber and the extraction chamber. The first condenser and the second condenser are located above the extraction tower, the first condenser is communicated with the extraction chamber, the second condenser is communicated with the rectification chamber, and by integrating the two functions of extraction and rectification, the tower achieves effective separation of acetic acid and water. The layered contact design ensures that the acetic acid raw material and the composite extraction agent are in full contact but not directly mixed between filler layers, the mass and heat transfer process is optimized, the extraction agent can be recycled after being treated by the rectification chamber, and the resource waste is remarkably reduced. And due to the recycling design, the operation cost is reduced, and the consumption of the extracting agent is reduced.

The invention provides high-activity crataegus pinnatifida fruit vinegar and a preparation method thereof.The preparation method comprises the steps that crataegus pinnatifida fruits are juiced, then a composite chelating agent composed of polyglucuronic acid and monopotassium phosphate is added for chelating treatment, and a polysaccharide-metal ion cross-linked network is removed; then adding endo-beta-1, 4-glucanase to carry out linear polysaccharide directional enzymolysis, so that the viscosity of the fruit juice is obviously reduced; after the components are adjusted, double modified yeast beta-glucan microspheres are added, yeast is inoculated for alcoholic fermentation, the microspheres are subjected to double grafting modification through galactooligosaccharide and theaflavin, and active components in the crabapple can be directionally captured and stabilized through hydrophilic and hydrophobic effects respectively; after alcohol fermentation is finished, acetic bacteria are inoculated for acetic fermentation, and finally, filtering and sterilization are performed. According to the method disclosed by the invention, by virtue of a synergistic strategy of breaking a network firstly, then carrying out enzymolysis debonding and then carrying out microsphere loading, the fermentation mass transfer efficiency is effectively improved, and meanwhile, the retention rate of functional components such as polyphenol and saponin in the fruit vinegar is increased.

This application provides a method for preparing pH-responsive polyamino acid drug-loaded nanoparticles based on sarcosine and their application. Sar-NNCA, L-lysine-NCA, and L-phenylalanine-NCA are dissolved in anhydrous DMF and polymerized under argon protection with a hexamethyldisilazine initiator to obtain a polyamino acid block copolymer Sar-NNCA. 80 -Lys(Cbz) n -Phe 10 The crude product was deprotected under HBr / acetic acid and loaded with DOX to obtain Sar. 80 -Lys n -Phe 10 -DOX. This application describes the preparation of a pH-responsive polymer nanoplatform, Sar, by encapsulating doxorubicin in polysarcosine-polylysine-polyphenylalanine nanoparticles via Schiff base bonds. 80 -Lys n -Phe 10 -DOX is used for tumor treatment. These polyamino acid nanoparticles are spherical with an average particle size of approximately 200 nanometers, exhibiting pH-responsive properties, excellent cellular uptake capacity, and anti-tumor effects. In vitro experiments show that Sar... 80 -Lys n -Phe 10 The carrier material exhibits excellent biocompatibility. The newly synthesized Sar... 80 -Lys n -Phe 10 -DOX nanomicelles show promise as a drug delivery system for cancer treatment.

The invention discloses a peach ketone synthesis method, which relates to the technical field of essence and perfume synthesis, and comprises the following steps: adding cyclopentanone and glacial acetic acid into a reaction container, starting stirring, adding manganese acetate (II) and cobalt acetate (II), and then adding acetic anhydride; introducing dried air below the liquid level, heating to 80-100 DEG C, dropwise adding D-limonene, and carrying out a heat preservation reaction after dropwise adding is finished; gC monitors the reaction endpoint, and the reaction is stopped when the product purity of the reaction liquid is not increased any more; after the reaction is finished, filtering, carrying out reduced pressure distillation to recover cyclopentanone, glacial acetic acid and unreacted D-limonene, after the recovery is finished, adding methanol and n-heptane into kettle liquid, carrying out water extraction, washing and liquid separation, washing an organic phase with a sodium bicarbonate aqueous solution, carrying out reduced pressure distillation on the organic phase to recover n-heptane, and after the recovery is finished, obtaining a peach ketone crude product; and carrying out crude drawing and rectification on the peach ketone crude product to obtain a peach ketone finished product. The method has the advantages of mild reaction conditions, low safety risk and few three wastes, and can obtain a high-purity peach ketone finished product.

The utility model provides an RO (Reverse Osmosis) acetic acid recovery system. The RO acetic acid recovery system comprises an RO water inlet buffer tank, a primary reverse osmosis membrane group and a secondary reverse osmosis membrane group, the liquid inlet end of the RO water inlet buffer tank is connected with an RO water inlet pipe; the output end of the buffer tank is sequentially connected with a reverse osmosis low-pressure pump and a reverse osmosis high-pressure pump through a conveying pipeline; the output end of the reverse osmosis high-pressure pump is connected with the primary reverse osmosis membrane group through a pipeline; one end, provided with a double-pipeline output end, of the second-stage reverse osmosis membrane group is connected with a liquid inlet end of the first-stage RO water production tank, and the other end of the second-stage reverse osmosis membrane group is connected with a liquid return opening of the RO water inlet buffer tank; the penetrating fluid conveying pump is connected with the sewage treatment device through an output pipeline; and a conveying pipeline connected with a liquid return port of the secondary RO water production tank is arranged on the output pipeline between the penetrating fluid conveying pump and the sewage treatment device. According to the RO acetic acid recovery system disclosed by the utility model, the recovery rate of acetic acid in water is improved, the consumption of fresh acetic acid is reduced and considerable economic benefits are brought to enterprises by arranging two groups of reverse osmosis membrane group operation loads.

The invention discloses a method for producing volatile fatty acid by improving microbial electro-catalytic conversion of CO2. The core of the method is to construct an efficient and stable heterozygote biological cathode. The method comprises the following steps: inoculating flora mainly comprising Acetobacter burgii and Desulfurization Vibrio in a cathode chamber of an H-type microbial electrosynthesis reactor, and adding a zinc source and a sulfur source to synthesize ZnS nanoparticles in situ on the surfaces of cells, so as to construct the bacterium and ZnS nanoparticle heterozygote biological cathode. The acetic acid yield of the heterozygote biological cathode can reach 3.38 mmol / L / d, and the accumulative yield of one batch is 20.25 mmol / L, which is 2.7 times that of an unmodified carbon cloth biological cathode; the coulombic efficiency of the total volatile fatty acid product reaches 96.47% and is 1.25 times that of a carbon cloth biological cathode; and the coulombic efficiency of acetic acid and the selectivity of acetic acid are respectively improved to 83.27% and 86.32%, which are obviously higher than 40.63% and 52.64% of those of a carbon cloth biological cathode. In addition, the charge transfer resistance of the heterozygote biological cathode is as low as 3.75 ohm and is obviously superior to that of a carbon cloth biological cathode (25.28 ohm), so that long-term stable and high-selectivity acetic acid production is realized.

The invention discloses a specific primer pair and a qPCR quantitative detection method for acetic acid bacteria in table vinegar, and particularly relates to the technical field of quantitative detection of acetic acid bacteria in table vinegar. The specific primer pair is designed by using a groEL gene as a target sequence and comprises a forward primer F and a reverse primer R, the sequence of the forward primer F is shown as SEQ ID No: 1, and the sequence of the reverse primer R is shown as SEQ ID No: 2. According to the qPCR quantitative detection method, accurate and rapid quantitative measurement of the concentration of acetic bacteria is realized through specific primer amplification, so that technicians can conveniently control the fermentation efficiency and the fermentation flavor of table vinegar.

The invention discloses a magnetic amide functionalized covalent organic framework material and a preparation method and application thereof, the preparation method comprises the following steps: S1, uniformly dispersing Fe3O4 nanoparticles and ammonia water, adding tetraethyl orthosilicate, and reacting; s2, a product obtained in the step S1 and 3-aminopropyltriethoxysilane are uniformly dispersed and subjected to a reaction, and aminated magnetic nanoparticles Fe3O4 (at) SiO2-NH2 are obtained; and S3, uniformly dispersing the Fe3O4 (at) SiO2-NH2 and trialdehyde phloroglucinol in a mixed solution of o-dichlorobenzene and N, N-dimethylformamide, adding 4, 4 '-diaminobenzanilide, uniformly mixing, then adding an acetic acid solution, and reacting at the temperature of 100-120 DEG C for 3-5 days to obtain the magnetic covalent organic framework Fe3O4 (at) Am-COFs material. The Fe3O4atAm-COFs prepared by the preparation method disclosed by the invention has a large specific surface area, rapid magnetic separation capability and abundant active sites, so that the adsorption performance of the material on a target object is effectively improved.

The invention relates to the technical field of acetic acid rectification, and discloses a glacial acetic acid rectification device with a purification function, the device comprises a distillation tower, a T-shaped ring is arranged in the distillation tower, the distillation tower and the tower wall form an annular flow channel, a central shaft is rotatably arranged at the bottom of the distillation tower, a conical cover is arranged at the top end of the shaft, and a condensation mechanism is arranged between the central shaft and the conical cover; a spiral stirring blade is arranged at the middle lower section of the central shaft, and the bottom end of the central shaft is in transmission connection with each reciprocating shaft through a swinging mechanism; by integrating chemical reaction purification, global stirring and dynamic condensation technologies, the rectification efficiency and the product purity are remarkably improved, long-term stable operation of the system is guaranteed through the purification and anti-scaling design, and the comprehensive performance is superior to that of traditional equipment.

The invention discloses a nutrition activator for improving fermentation acid production activity of acetic bacteria and application of the nutrition activator, and belongs to the technical field of food fermentation. The nutrition activator disclosed by the invention is prepared from the following nutritional ingredients in percentage by concentration: 1.00 to 1.50 percent of acetic acid, 1.00 to 2.00 percent of yeast extract, 1.50 to 2.50 percent of trehalose and 0.10 to 0.20 percent of CaCl2. The nutrition activator can improve the fermentation acid production activity of acetic acid bacteria, is a natural and safe nutrition activator, can activate the acetic acid bacteria, improves the activity of ethanol dehydrogenase and acetaldehyde dehydrogenase of the acetic acid bacteria in vinegar fermentation, improves the acid resistance of the acetic acid bacteria, and improves the fermentation efficiency and the acetic acid yield.

The application discloses a high-temperature inducible promoter and application thereof, and belongs to the field of genetic engineering and fermentation engineering. Acetobacter pasteurianus A high-temperature inducible promoter P icd is screened from a bacillus pabuli (Olsenella ) and has a nucleotide sequence as shown in SEQ ID NO. 1. The application further constructs a recombinant expression vector pBBR1MCS2-icd containing the promoter and a recombinant bacterium expressing an ethanol dehydrogenase gene AdhP. The recombinant strain ApAdhP(icd) using the promoter to regulate the expression of the ethanol dehydrogenase can not only reduce the metabolic burden of the cell and maintain a good growth trend under high-temperature fermentation conditions, but also can reach an acetic acid final yield of 52.65 g / L, which is increased by about 31.5% compared with a wild strain. The application effectively solves the problems of the decrease of acetic acid production rate and yield under high-temperature environment in summer in traditional acetic acid fermentation.

The invention belongs to the technical field of genetic engineering, and discloses a genetically engineered bacterium for efficiently synthesizing gamma-aminobutyric acid from the beginning by efficiently utilizing a mixed carbon source, a method and application, and the genetically engineered bacterium is obtained by overexpressing edd from Zymomonas mobilis on the basis of escherichia coli E. coli G16; eda from the zymomonas mobilis is over-expressed; performing overexpression on acs (Acetobacter pasteurianus) sources; a malic acid synthase gene aceB is knocked out; a malic acid synthase gene glcB is knocked out; and the acetyl phosphate transferase gene eutD is knocked out. The escherichia coli strain which is clear in genetic background and capable of producing gamma-aminobutyric acid is utilized, and an ED path and an acetic acid utilization path are introduced, so that acetyl CoA branch metabolism is reduced, and the yield and conversion rate of gamma-aminobutyric acid are remarkably improved.

The application discloses a compound amino acid injection (15-HBC) composition and a preparation method thereof, and belongs to the technical field of medicines. The compound amino acid injection (15-HBC) composition provided by the application comprises the following components in an amount of 250mL of the composition: isoleucine 1.532-2.298g, leucine 2.756-4.134g, lysine acetate 1.160-1.740g, methionine 0.500-0.750g, phenylalanine 0.640-0.960g, threonine 0.400-0.600g, tryptophan 0.180-0.270g, valine 1.772-2.658g, alanine 0.800-1.200g, arginine 1.160-1.740g, histidine 0.320-0.480g, proline 1.260-1.890g, serine 0.660-0.990g, glycine 0.660-0.990g, cysteine hydrochloride 0.040-0.060g, and appropriate amount of glacial acetic acid for pH adjustment, and the rest is injection water. The application also provides a preparation method of the compound amino acid injection (15-HBC) without sulfite antioxidant. Meanwhile, the activated carbon adsorption process is removed in the preparation, the control of raw material packages, production processes and the like is strengthened to control bacterial endotoxins, the production process is simplified, and the product quality is ensured.

This application discloses a method for synthesizing polyethylene (2,5-furandicarboxylate), comprising the following steps: mixing 2,5-furandicarboxylic acid and ethylene glycol in a molar ratio of 1:2, adding zinc acetate as an esterification catalyst, and carrying out an esterification reaction at 195°C and a pressure not exceeding 0.3 MPa until the water content reaches 98% of the theoretical amount; adding antimony trioxide as a polymerization catalyst and triphenyl phosphate as a stabilizer to the esterification product, and reacting for 1 hour at a low vacuum of 800-1000 Pa and a temperature of 215°C to carry out pre-condensation; and carrying out a condensation reaction of the pre-condensation product at a high vacuum of less than 200 Pa and a temperature of 235°C until the torque reaches 4.2 A / 65 rpm to obtain polyethylene (2,5-furandicarboxylate). By reducing the amount of antimony trioxide to 0.045% and triphenyl phosphate to 0.1%, the amount of stabilizer was reduced, releasing catalyst activity. This shortened the polymerization time from 300 min to 230 min, reduced the color value b from 12-13 to 10, and improved the intrinsic viscosity to 0.507~0.517 dL / g. This achieved a reduction in production costs and heavy metal residues while improving the product's color.

The invention belongs to the technical field of oil exploitation, and particularly relates to a composite blocking remover and a preparation method and application thereof. The composite blocking remover comprises the following components in percentage by weight, comprising the following components in percentage by weight: 5 to 8 percent of hydrochloric acid, 1 to 3 percent of fluoboric acid, 0.5 to 2 percent of acetic acid, 0.5 to 1 percent of dodecyl trimethyl ammonium chloride, 0.1 to 0.3 percent of potassium chloride, 0.3 to 0.8 percent of sodium erythorbate, 0.2 to 0.5 percent of tetrasodium glutamate diacetate, 1 to 2 percent of ethylenediamine tetraacetic acid, 0.1 to 0.2 percent of inositol hexaphosphate, 0.1 to 0.2 percent of cocoanut fatty acid diethanolamide, 0.3 to 0.5 percent of sodium dodecyl benzene sulfonate, 0.3 to 0.5 percent of dimethyl diallyl ammonium chloride, 0.1 to 0.2 percent of ethylene glycol monobutyl ether and the balance of water. The composite blocking remover provided by the invention does not generate the problem of secondary precipitation, slows down the reaction speed of acid rocks, effectively pushes a blocking removal measure solution into the deep part of a stratum, enlarges the blocking removal radius, realizes deep treatment of the stratum, improves the acidification effect, and prolongs the effective action time of acidification.

The invention discloses a method for preparing acetonitrile by an acetic acid ammoniation method, and belongs to the technical field of chemical engineering. In the invention, the inlet low-temperature area promotes ammoniation of acetic acid to generate acetamide, thereby avoiding catalytic polymerization of acetic acid; acetamide is dehydrated to generate acetonitrile in the middle-section medium-temperature zone; and the lower-end high-temperature area preferentially completes deep conversion of unreacted substances. Axial temperature gradient distribution is formed in the fixed bed reactor, the reaction process is optimized, and the problems of excessive reaction, hot spot out-of-control and low selectivity caused by traditional overall heating are solved. According to the method for preparing acetonitrile by the acetic acid ammoniation method based on segmented temperature control, the utilization rate of raw materials is increased, side reactions are reduced, the yield of acetonitrile is increased to 99% or above, the yield of series of impurities such as acetone and acetamide is lower than 1%, and the cost of a subsequent purification process is reduced; meanwhile, the catalyst coking probability is reduced, and the service life of the catalyst is prolonged.

The application discloses a sterone C1,2 position dehydrogenase mutant and application thereof in synthesis of acetic acid tetraen. The application mines and modifies the sterone C1,2 position dehydrogenase by molecular biology technology and protein engineering technology, obtains a mutant with excellent properties, and the amino acid sequences of the sterone C1,2 position dehydrogenase mutant are shown as SEQ ID NO:5-SEQ ID NO:12. Under the participation of hydrogen donor and oxygen, the sterone C1,2 position dehydrogenase mutant can catalyze the oxidation of 60g / L acetic acid trien to acetic acid tetraen, the conversion rate reaches 99.1% within 10h, and the space-time yield reaches 5.94g / L / h. The application has the advantages of green safety, short reaction cycle, simple purification, low cost and the like, and has an excellent application prospect.

The utility model relates to the technical field of chemical separation, in particular to a liquid-liquid extraction tower for separating acetic acid from water, which comprises an extraction tower, an extraction agent feed port arranged at the bottom end of the extraction tower, a raw material feed port arranged at the top end of the extraction tower, an extraction phase discharge chute arranged at the top end of the extraction tower and an extraction phase discharge port arranged at the top end of the extraction tower. An upper U-shaped bent pipe is mounted on a discharge port of the extraction box, an extracted mixture forms an extraction phase at the top of the tower, and the extraction phase is collected through the extraction phase discharge groove and discharged through the extraction phase discharge port. And an upper U-shaped bent pipe is mounted on the extraction phase discharge port, so that the waste water is prevented from being carried to the extraction phase discharge port, the unextracted residual solution is prevented from being formed at the bottom of the tower, and the residual solution is collected through the raffinate phase discharge groove and then discharged through the raffinate phase discharge port. And a lower U-shaped bent pipe is arranged on the raffinate phase discharge hole, so that the extracting agent is prevented from overflowing from the tower kettle.

The invention discloses 1, 3-bifunctional isobutyramide pyridinium salt, a preparation method and application of the 1, 3-bifunctional isobutyramide pyridinium salt in antiviral drugs, and belongs to the field of drug synthesis. The preparation method comprises the following steps: under the protection of argon, by taking (3-(2-fluorophenyl)-2, 2-dimethyl propionyl) (pyridine-2-yl) amide as a raw material and dichloroethane as a solvent, adding palladium acetate, iodobenzene diacetate, water and acetic acid, and reacting to obtain 1, 3-hydroxyl / acetoxy pyridinium salt; the preparation method comprises the following steps: by taking N-(3-((2-(2-fluoro-4-phenylbiphenyl-3-yl)-2-methylpropionyl) oxy)-2, 2-dimethyl propionyl) pyridinium as a raw material and toluene as a solvent, adding palladium acetate, 4, 5-diazafluorene-9-one, iodobenzene diacetate, potassium carbonate and acetic anhydride, and reacting to obtain the 1, 3-diacetoxypyridinium salt. The synthesis process of the 1, 3-bifunctional isobutyramide pyridinium salt is simple and convenient, and the reaction conditions are mild; meanwhile, the pyridinium salt has good anti-enterovirus activity and is expected to become a medicine for effectively treating EV-A71 infection.

The invention relates to the technical field of biomedical materials and drug delivery, in particular to a chitosan drug-loaded microsphere based on an ionic crosslinking self-assembly synergistic mechanism and a preparation method thereof.The preparation method comprises the steps that chitosan is dissolved in an acetic acid aqueous solution, a drug and an amphiphilic block copolymer are added to form a primary self-assembly structure, and the primary self-assembly structure is prepared; and dropwise adding sodium tripolyphosphate to trigger ionic crosslinking and secondary self-assembly, and finally purifying and freeze-drying to obtain a microsphere finished product. The microspheres are uniform in particle size, high in encapsulation efficiency, excellent in slow release performance and suitable for anti-cancer drugs, protein drugs and vaccine carriers, the preparation process is carried out in a pure water phase system, no organic solvent or complex equipment is needed, the process is simple and convenient, the cost is low, the amplification performance is high, and the good industrialization prospect is shown.

The invention discloses a cholate synergistic effect-based open wound medicine formula and a preparation method thereof, cholate and sodium chloride are subjected to high-pressure homogenization and grinding to nanoscale, acetic acid, water and alcohol are sequentially added, after pH is adjusted, terminal filtration is carried out through a 0.22-micron filter membrane, sterility is ensured, the formula can penetrate into a corium layer to inhibit inflammatory factors (such as TNF-alpha), and the open wound medicine has a good application prospect. And an epidermal cell migration signal (such as TGF-beta 1) is activated, so that the healing period is shortened to 8-10 days. The nano-scale dispersion improves the medicine permeation efficiency, the weak acid environment inhibits the formation of a gram-positive bacterium biofilm, and the eye drops are free of sensitization, are suitable for immunodeficiency patients and have auxiliary effects on haemorrhoids and eye diseases.

The invention discloses an HPLC (High Performance Liquid Chromatography) detection method for the content of acetic acid impurities in aspirin-DL-lysine for injection, and belongs to the technical field of pharmaceutical analysis. Comprising the following steps: S1, mixing an acetic acid impurity reference substance, a phosphoric acid solution and a dissolving solvent to obtain a reference substance solution; mixing an aspirin-lysine test sample for injection, a phosphoric acid solution and a dissolving solvent to obtain a test sample solution; mixing the aspirin-DL-lysine test sample for injection, the phosphoric acid solution, the acetic acid impurity reference substance and the dissolving solvent to obtain a test sample labeling solution; and S2, performing chromatographic analysis, performing gradient elution by taking octadecylsilane chemically bonded silica as a filler, a phosphoric acid solution A as a mobile phase A and methanol as a mobile phase B, and calculating the content of the acetic acid impurity in the aspirin-DL-lysine for injection. According to the method, a common high performance liquid chromatograph is adopted for chromatographic analysis, and a conventional chromatographic column is used for gradient elution, so that acetic acid impurities are eluted in a very short time, the analysis and detection time of a sample is shortened, and the time and the production cost are saved.