42 results about "Teriparatide" patented technology

The invention discloses novel parathyroid hormone (PTH) (1-34) mimetic peptide based on protein domain reconstruction.; The mimetic peptide contains peptide fragment repeats at 29-34th positions, andthe 34th position of the mimetic peptide and the last amino acid of the peptide fragment repeats at 29-34th positions are changed into tyrosine (Tyr, Y) from phenylalanine (Phe, F), so that MY-3 peptide is formed; preclinical experiments show that the MY-3 mimetic peptide can promote osteoblast differentiation, and has a very good effect of treating osteoporosis and promoting bone growth; the anti-osteoporosis effect of the MY-3 mimetic peptide is better than that of the PTH (1-34) (a product on the market is named as Forteo or Teriparatide), so that the MY-3 mimetic peptide has a significantclinical application prospect.

The invention relates to the field of medicine synthesis, and discloses a method for synthesizing teriparatide. The method comprises the following steps: performing esterification reaction between Phe coupled with a protecting group at an N end and HMP Linker resin under the action of a coupling reagent and an activating reagent to obtain peptide resin 1; and performing one-by-one extension coupling on the rest protective amino acids under the reaction of a condensation reagent and an activating reagent according to a sequence from a C end to the N end of a teriparatide amino acid sequence to obtain corresponding peptide resin after each extension coupling and finally obtain teriparatide resin, performing acid hydrolysis to obtain a teriparatide crude product, and purifying the teriparatide crude product into acetate so as to obtain a teriparatide pure product. According to the method disclosed by the invention, a suitable synthesis scheme is selected, the HMP Linker resin is used as carrier resin, the whole synthesis process is optimized, a high-purity product is obtained, the total yield of the teriparatide is improved, and the method is harmless to any environment.

The present invention relates to a method for preparing teriparatide. The specific steps of the present invention are: A) in the presence of an activator system, coupling the resin solid phase carrier and Fmoc-Phe-OH to obtain Fmoc-Phe-resin; B ) Through the solid-phase synthesis method, amino acids with N-terminal Fmoc protection and side chain protection are sequentially coupled according to the peptide sequence of the teriparatide main chain. The amino acid at position 16-17 of the peptide sequence is a pseudo-proline dipeptide Fmoc-Asn ( Trt)-Ser(ψMe,Me Pro)-OH replaces the original two amino acids at 16-17 positions for coupling; C) Peptide resin cleavage uses NH-containing 4 I / Me 2 The lysate of the TFA system of S, the crude product was purified by HPLC and freeze-dried to obtain teriparatide, whose process impurity Met 8 (O)‑Teriparatide content is less than 0.1%. The invention provides a teriparatide preparation process with high purity, low cost and suitable for large-scale production. This process can effectively control Met 8 (O)-Teriparatide content does not affect the overall yield of teriparatide.