24 results about "Acetobacter" patented technology

The invention belongs to the technical field of microorganisms and fermentation, and discloses a method for synergistically fermenting ginseng powder through bacteria and enzymes, acetobacter aceti, ginseng spice and application, and the method comprises the following steps: (1) preparing a ginseng powder liquid culture medium; (2) inoculating acetobacter aceti and cellulase, and performing liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuously fermenting for 96-120 hours; and (4) after fermentation is finished, supplementing ethanol until the final volume concentration of ethanol is 45-55%, stirring at normal temperature for 24 hours, standing, settling and filtering to obtain supernate, namely the ginseng spice. According to the preparation method, the earthy smell of the traditional ginseng spice can be remarkably reduced by the obtained ginseng spice through technologies such as bacterium-enzyme synergy and specific microbial fermentation metabolism control, meanwhile, the ginseng spice has typical aroma characteristics and is high in suckability, and meanwhile, the product quality of ginseng spice series cigarettes is effectively improved.

This invention belongs to the field of vinegar brewing technology, and provides a multi-strain co-fermentation selenium-enriched active probiotic functional vinegar and its preparation method. Jujube enzymatic hydrolysate, malt juice, and ultrafine powder of Astragalus-Codonopsis compound traditional Chinese medicine are mixed in a certain proportion, and sodium selenite is added. Alcoholic fermentation is carried out using Saccharomyces cerevisiae CGMCC 34906. After alcoholic fermentation, acetic acid and lactic acid fermentation is carried out using a compound inoculum of Lactobacillus plantarum and Acetobacter pasteurization. After fermentation, the mixture is centrifuged, filtered, sterilized, post-ripened, blended, and sterilized. Active selenium-enriched Lactobacillus plantarum microcapsules are added, resulting in a double enhancement of selenium enrichment and active probiotics, yielding a selenium-enriched active probiotic functional vinegar. The vinegar is reddish-brown in color and has a complex aroma of jujube and malt fermentation, along with the aroma of traditional Chinese medicine. It has a mellow and pure sour taste with a long aftertaste. The flavor is rich and pleasant; total selenium ≥ 0.10 mg / L, and the number of viable lactic acid bacteria ≥ 10. 7 CFU / mL, polysaccharides ≥60.00 mg / 100 mL, saponins ≥2.50 mg / 100 mL.

The invention discloses Acetobacter peach and application thereof in prevention and treatment of postharvest diseases, and belongs to the technical field of microorganisms and the field of biological prevention and treatment of postharvest diseases. The Acetobacter persici NM-1 disclosed by the invention has an antagonistic effect on telosporium roseum and alternaria alternata, and has a remarkable prevention and treatment effect on postharvest diseases of apples such as red rot and black spot. The invention provides a biocontrol bacterium resource for prevention and treatment of apple red rot and black spot.

The invention discloses a method for enhancing the flavor of sea buckthorn fruit vinegar through synergistic fermentation of multiple strains combined with medicinal and edible raw materials. Wickerhamomyces anomalus YCJP320 with clear hereditary characteristics and proprietary intellectual property rights (CGMCC No. 33925, which can produce esterifying enzyme at high yield and increase aroma components such as ester components) and acetobacter pasteurianus CL247, CGMCC No. 33924, which can resist 18% ethanol and 7% acetic acid, and can produce acid efficiency are respectively used for alcoholic fermentation and acetic fermentation, and then fermentation is carried out, so that the fermentation efficiency is improved. According to the fermentation production process of the sea buckthorn fruit vinegar, ganoderma lucidum post-prebiotics, phellinus linteus post-prebiotics and medicinal and edible raw materials (astragalus membranaceus, dogwood and cinnamon) are used as fermentation culture medium components for nutrient enrichment, so that directional regulation and control of flavor substances and functional components of the sea buckthorn fruit vinegar are realized.

This invention discloses a strain of *Pediococcus pentosaceus* RL-10 and its application in bio-acidification rice soaking and bacterial cellulose production, belonging to the field of microbial technology. The preservation number of *Pediococcus pentosaceus* RL-10 is CCTCC NO: M 20252428. This invention is the first to use *Pediococcus pentosaceus* as a strain for bio-acidification rice soaking. Using rice soaking water as a production substrate, *Acetobacter Hansenulatus* and strain RL-10 are co-cultured in situ to produce bacterial cellulose loaded with antimicrobial peptides. Under certain process conditions, acidification of rice soaking by strain RL-10 can rapidly increase acidity and shorten soaking time. The bacterial cellulose production process of this invention is simple and low-cost; verification shows that the bacterial cellulose prepared by the method of this invention has high yield and good antibacterial activity, meeting the requirements of large-scale industrial production.

The invention belongs to the technical field of ink, and particularly relates to flash watercolor ink and a preparation method thereof. The raw materials comprise water, PEG, a dispersing agent, aluminum shimmering powder, a penetrating agent, a suspending agent, acrylic resin, a mildew preventive and dye. Wherein the suspending agent comprises at least one of a bacterial cellulose suspension, a modified bacterial cellulose suspension and microcapsule powder, the bacterial cellulose suspension is obtained by fermenting acetobacter aceti, and the modified bacterial cellulose suspension is obtained by modifying the bacterial cellulose suspension. The preparation method comprises the following steps: weighing the raw materials in parts by weight; adding water, PEG, a dispersing agent and a penetrating agent into a stirring container, and uniformly stirring to obtain a liquid phase; adding acrylic resin into the liquid phase, continuously stirring, adding a suspending agent, stirring, adding aluminum glittering powder, dye and a mildew preventive, adjusting the pH, uniformly stirring, and adjusting the viscosity to obtain the glittering watercolor ink. The ink disclosed by the invention is not easy to generate dead precipitation and mildew, and can be used for writing smoothly.

The application belongs to the technical field of bioengineering, and discloses a gene engineering bacterium of acetobacter pasteurii with high temperature resistance, a construction method and application thereof. Acetobacter pasteurianus The gene engineering bacterium is constructed by using the acetobacter pasteurii (ATCC 10137) as a starting strain and knocking out the transposase gene in the genome of the acetobacter pasteurii (ATCC 10137) by a homologous recombination method. TnsR The gene engineering bacterium with high temperature resistance is obtained. TnsR The deletion of the gene is beneficial to maintaining the stability of the genome, so that the strain can maintain better growth and metabolic capacity under high-temperature pressure. Experimental results show that the biomass, survival rate and acetic acid yield of the gene engineering strain under the condition of 40 DEG C high temperature are significantly better than those of the wild-type strain. When the gene engineering strain is applied to traditional solid-state food vinegar brewing, the yield of organic acids such as acetic acid is significantly improved, the content of ester flavor substances is greatly improved, and the flavor quality of food vinegar is effectively improved. The application provides an effective technical means for solving the problem that high temperature in summer leads to abnormal fermentation and yield reduction of food vinegar.

This invention discloses a method for preparing rum with high total ester content. The method includes the following steps: pretreating molasses; preparing a first fermentation medium using the pretreated molasses; expanding the culture of fissile yeast seed liquid in the first fermentation medium to obtain fissile yeast fermentation broth; continuously adding the pretreated molasses to the fissile yeast fermentation broth, culturing to obtain a fermentation product, wherein the residual sugar content of the fermentation product is controlled at 0.5-2%; preparing a second fermentation medium using the pretreated molasses; inoculating the fermentation bacteria into the second fermentation medium, culturing to obtain a fermentation broth; the fermentation bacteria include at least one of Clostridium acetic acid and Acetobacter pasteurella; adding the fermentation broth to the fermentation product, culturing to obtain a mash; continuously distilling the mash, using a kettle heating method for three-part distillation, mixing the second fraction with oak shavings, and aging; obtaining the base spirit, which is then blended to obtain rum with high total ester content. This method can produce mash with high alcohol content and high ester content in a short time.

The invention provides a composition containing acetobacter saxatilis and pantoea azototrophicus and application of the composition. The composition provided by the invention comprises acetobacter sativus or progeny thereof and pantoea azototrichum or progeny thereof; the registration number of the acetobacter breviscapus in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36149; the registration number of the nitrogen-fixing pantoea in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36150. The invention further discloses a preparation method of the nitrogen-fixing pantoea. The acetobacter sativus and the nitrogen-fixing pantoea or the composition of the acetobacter sativus and the nitrogen-fixing pantoea can be used for reducing emission of nitrogen-containing gas in the livestock breeding process and the livestock manure (normal state and composting) treatment process, and has a good application prospect.

An air pressure control system in a fermentation tank for table vinegar production comprises an air pressure control box, an air pump is arranged at the top in the air pressure control box, an air outlet of the air pump is communicated with the top of the fermentation tank through a pipeline, a through groove is formed in the bottom of the air pressure control box, and the top of the fermentation tank is communicated with the top of a vinegar liquid storage tank through a pipeline. A vent electronic valve is arranged on a communicating pipeline between the top of the fermentation tank and the bottom of the vinegar liquid storage tank, the bottom of the fermentation tank is communicated with one end of a discharge pipe, the other end of the discharge pipe is communicated with the top of the vinegar liquid storage tank, a discharge electronic pump and a discharge electronic valve are arranged on the discharge pipe, and the discharge electronic pump is communicated with the discharge electronic valve. An electronic barometer communicated with the interior of the fermentation tank is arranged on the fermentation tank, and a control panel is arranged on the air pressure control box. The air pressure in the fermentation tank can be automatically controlled, so that the oxygen content in the fermentation tank is ensured, manual treatment is not needed, the operation is simple, and the growth efficiency and production safety of acetobacter aceti in the fermentation tank can be ensured.

The invention discloses acetobacter Indonesii with low fusel oil yield and an application of the acetobacter Indonesii. The strain of the Acetobacter indicum X3 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is July 15, 2025, and the preservation number is CCTCC NO.M20251613. The strain of the Acetobacter indicum X3 has the advantages that the strain of the Acetobacter indicum X3 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO.M20251613; the acetobacter strain is obtained by separating and purifying mash of the rice-flavor liquor, fusel oil in the rice-flavor liquor can be reduced by 14.2%, the acetobacter seed solution and distiller's yeast of the rice-flavor liquor are prepared into mixed yeast to be applied to brewing of rice-flavor raw liquor, the total acid content in a produced liquor body reaches 0.35 g / L and is increased by 66.7% compared with that of a production control group, and the production cost is reduced. The total ester content reaches 0.35 g / L and is improved by 47.8% compared with that of a control group, and the flavor purity and the comprehensive score of a wine body are optimal through sensory evaluation.

The invention relates to acetobacter breviscapus GJ1-4, a microbial agent of the acetobacter breviscapus GJ1-4 and application of the acetobacter breviscapus GJ1-4 in promoting acidification of kitchen waste and biogas production, and belongs to the technical field of microorganisms The Acetobacter cereus GJ1-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32450, the preservation date is October 31, 2024, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the acetobacter breviscapus GJ1-4 in promotion of acidification and biogas production of kitchen waste. The acetobacter breviscapus GJ1-4 provided by the invention can efficiently grow in a pH range of 4.0-9.0, has the characteristics of efficient acid production, high ammonia nitrogen resistance and good culture, and can effectively promote acidification of kitchen waste and production of biogas.

The present application belongs to the field of microorganism and fermentation technology, and discloses a method for synergistically fermenting ginseng powder by bacteria and enzymes, and fructobacillus, ginseng spice and application. The method comprises the following steps: (1) preparing ginseng powder liquid medium; (2) inoculating fructobacillus and cellulase, and liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuing fermentation for 96-120 hours; (4) after the fermentation is completed, supplementing ethanol to a final volume concentration of 45%-55%, stirring at room temperature for 24 hours, standing and settling, and filtering to obtain supernatant, which is ginseng spice. Through the synergistic fermentation of bacteria and enzymes and the specific microbial fermentation metabolism control technology, the ginseng spice obtained by the present application can significantly reduce the earthy smell of traditional ginseng spice, has typical aroma characteristics, has strong absorbability, and effectively improves the product quality of ginseng spice series cigarettes.

The invention belongs to the technical field of application of microorganisms, and particularly relates to a diazo nutritional type acetobacter gluconicum mutant, a nitrogen-fixing microbial agent, a preparation method of the nitrogen-fixing microbial agent and application of the nitrogen-fixing microbial agent. The mutant strain with high nitrogenase activity and genetic stability is obtained through ARTP mutagenesis screening, the nitrogen fixation effect is remarkably improved, and the nitrogenase activity of the mutant strain on a nitrogen-free culture medium reaches 368.84 nmol.mg <-1 >. H <-1 >. The nitrogen-fixing bacterial strain disclosed by the invention can effectively improve index values such as plant height, root length and plant weight of rice, has a good growth-promoting effect on various crops, and is a broad-spectrum growth-promoting nitrogen-fixing bacterial strain. The nitrogen-fixing bacterial strain can be rapidly cultured in a short time, and is suitable for industrial production. The diazo nutritional glucose acetobacter mutant is a high-enzyme-activity mutant strain obtained through ARTP mutagenesis and acetylene reduction method screening and has good nitrogen fixation capacity, the nitrogenase activity of the diazo nutritional glucose acetobacter mutant is improved by 3.1 times, and the hereditary stability of the diazo nutritional glucose acetobacter mutant is good after multiple times of subculture.

The invention relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the invention comprises the following components in parts by weight: 1-8 parts of a saw palmetto extract, 1-5 parts of an inonotus obliquus extract, 0.5-2.5 parts of a rhizoma dioscoreae nipponicae extract, 20-30 parts of a raspberry extract, 3-5 parts of a cortex erythrinae extract, 3-5 parts of compound bacteria and 10-30 parts of a carrier, furthermore, the compound bacteria are selected from enterococcus faecium, bacillus laterosporus, acetobacter ollani, lactobacillus acidophilus and lactobacillus rhamnosus; the invention also provides an animal feed prepared from the compound feed additive. The compound feed additive provided by the invention can improve the growth performance of animals from multiple aspects of enhancing animal immunity, improving animal intestinal microbe balance, increasing the feed conversion rate and the like, so that the breeding benefits of the livestock and poultry breeding industry are improved.

This application discloses a method for the synergistic application of algae and bacteria in the treatment of livestock and poultry wastewater, relating to the field of livestock and poultry wastewater treatment technology. The application method includes the following steps: wastewater pretreatment; bacterial strain compounding: Enterococcus hesperidinus, Rhodococcus roseus, Pantotheca cumulus, and Acetobacter cocoa are fermented individually, and then the single fermented bacterial strains are compounded. The mass ratio of the fermentation liquid of Enterococcus hesperidinus, Rhodococcus roseus, Pantotheca cumulus, and Acetobacter cocoa is (1-3):1:(1-3):(3-5) to obtain a compound bacterial agent; microalgae compounding: Chlorella ellipsoides and Scenedesmus are cultured individually at a light intensity of 2500-4000 lux for 5-10 days at a temperature of 25-28℃, and then the single cultured microalgae are compounded to obtain a compound microalgae agent. The technical solution provided in this application can effectively degrade various pollutants in livestock and poultry wastewater, with significant treatment effects.

The application relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the application comprises the following components in parts by weight: saw leaf palm extract 1-8 parts, white birch mushroom extract 1-5 parts, Rhizoma Anemarrhenae extract 0.5-2.5 parts, raspberry extract 20-30 parts, sea-tortoise skin extract 3-5 parts, compound bacteria 3-5 parts and carrier 10-30 parts; further, the compound bacteria are selected from Enterococcus faecium, Bacillus laterosporus, Acetobacter orleanensis, Lactobacillus acidophilus and Lactobacillus rhamnosus; and the application also provides animal feed prepared from the compound feed additive. The compound feed additive provided by the application can improve the growth performance of animals from aspects of enhancing the immunity of animals, improving the intestinal microorganism balance of animals, improving the feed conversion rate and the like, so as to improve the breeding benefit of livestock and poultry breeding.

The invention belongs to the technical field of microbial fermentation, and particularly relates to acetobacter luviangensis and application thereof in shortening the growth period of crops in a rice-wheat rotation system. The invention discloses an Acetobacter luvianense JY1053 strain, a preparation method of a microbial agent of the Acetobacter luvianense JY1053 strain, and application of the Acetobacter luvianense JY1053 strain in shortening the growth period of crops in a rice-wheat crop rotation system. The microbial agent can significantly shorten the growth periods of rice and wheat and increase the yield of rice and wheat, compared with conventional fertilization, the growth period in the rice season is shortened by 9 days, the yield is increased by 8.97%, the growth period in the wheat season is shortened by 5 days, and the yield is increased by 6.35%. In addition, the Acetobacter luwanensis JY1053 has a relatively good antagonistic effect on fusarium oxysporum cucumber specialized type, botrytis cinerea, alternaria tenuissima and fusarium solani, and the Acetobacter luwanensis JY1053 has a relatively good antagonistic effect on fusarium oxysporum cucumber specialized type, botrytis cinerea, alternaria tenuissima and fusarium solani.

This invention discloses a method for producing ethanol by fermentation of Saccharomyces cerevisiae based on a dual antimicrobial strategy. The method involves inhibiting the respiratory chain of contaminating microorganisms with cyanamide and utilizing the overexpression of the gene encoding cyanamide hydratase in Saccharomyces cerevisiae 1308. CAH To reduce the impact of cyanamide on Saccharomyces cerevisiae, this invention employs the antimicrobial peptide DptB and the endolysin LysKB317 to target and inhibit Acetobacter and Lactobacillus. This invention combines the chemical antimicrobial properties of cyanamide with the biological antimicrobial properties of the endolysin, forming a dual antimicrobial strategy in the fermentation process of Saccharomyces cerevisiae for ethanol production. The synergistic effect of chemical and biological antimicrobial action achieves an organic combination of broad-spectrum and targeted antimicrobial activity. This effectively inhibits contaminating bacteria while reducing interference with Saccharomyces cerevisiae metabolism caused by chemical antimicrobial agents, thus lowering the risk of contamination during industrial ethanol production. Ultimately, it improves the stability and economic efficiency of ethanol production, increasing the ethanol yield of the 50–200 g / L glucose fermentation system by 10.1%–23.7% compared to the control group.

This invention discloses a method for preparing a mulberry-monk fruit fermented beverage, belonging to the field of microbial fermentation technology. The method includes raw material processing, alcoholic fermentation, acetic acid fermentation, and blending steps: Monk fruit is extracted using a combination of juice extraction and hot water countercurrent extraction; the extract is kept warm to improve clarity and inactivate enzymes; using mulberry juice and monk fruit juice as substrates, fruit wine yeast is inoculated for anaerobic fermentation; then, monk fruit juice and edible alcohol culture are added to adjust the alcohol content, followed by aerobic fermentation with *Acetobacter acetosporum* and acetic acid bacteria; finally, the fermented juice, glycoside-containing monk fruit juice, mulberry concentrate, and erythritol are blended to prepare the beverage. This invention fully utilizes mulberry and monk fruit resources, retains active ingredients such as polyphenols, anthocyanins, and polysaccharides, and produces a low-calorie, flavorful, and safe product suitable for industrial production, possessing both nutritional and health benefits.

The inactivated lactic acid bacteria and the metagen thereof disclosed by the invention can be used for preparing a product for inhibiting helicobacter pylori. Besides, the pediococcus acidilactici Lab2 metagen and the acetobacter aceti AS1.41 metagen are compounded, it is found that compounding of the pediococcus acidilactici Lab2 metagen and the acetobacter aceti AS1.41 metagen is remarkably higher than that of single Lab2 metagen or acetobacter aceti metagen, and in the ethanol metabolism level, the compound metagen remarkably improves ADH and ALDH activity of the mouse liver. Secondly, on the oxidative stress regulation level, the SOD, CAT activity and GSH content of the mouse liver are remarkably improved by compounding the metabiotics, and meanwhile, the MDA level is reduced. In the aspects of liver function protection and lipid metabolism regulation, the compound metabiotics can significantly reduce the activity of ALT and AST in serum, and can significantly reduce the level of TG and TC in serum at the same time. The liver tissue pathological section further proves that the composite metagen can obviously relieve hepatocyte edema and microvesicle fatty degeneration, recover normal structural arrangement of the liver tissue and realize comprehensive protection on the alcoholic acute liver injury.

The present invention provides a composition that extends lifespan and / or reduces or delays the onset of at least one age-related symptom or condition in a subject. In some embodiments, a composition comprises at least one bacterial strain or an extract or component thereof and an excipient. In some embodiments, at least one bacterial strain comprises the genus Gluconobacter, Acetobacter, Gluconacetobacter, Acidimonas, Plurashibacter, Asia, Granobacter, Cozecia, NeoAsia, NeoKacia, Gulgarobacter, Granobacter, Cozecia, Chrysanthemum, or a combination thereof. The invention also relates to a method for preparing the bacterial strain. The method includes selecting at least one bacterial strain from the bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, and selecting at least one bacterial strain. Methods of making and using the compositions disclosed herein are also provided.

The invention discloses a nanometer platform based on manganese prussian blue nanometer enzyme and acetobacter aceti as well as a preparation method and an application method of the nanometer platform. The nano-platform is obtained by mixing a manganese prussian blue nano-enzyme solution and acetobacter aceti, wherein the ratio of manganese prussian blue nano-enzyme to acetobacter aceti is 1 * 10 < 8 > (CFU / mL): (25-400) (g / mL). The acetobacter aceti is cultured by ethanol, and growth and ethanol metabolism are optimized. According to the manganese prussian blue nano-enzyme, the structure stability and dispersity are optimized by adjusting the proportion of manganese and iron precursors. The nano platform can be used for preparing oral medicines for preventing and treating acute alcoholic liver injury, or can be used as an imaging contrast agent of MRI (Magnetic Resonance Imaging) for observing gastrointestinal tract distribution. The nano platform has the advantages of simple preparation process, wide application range, good imaging performance, excellent oxidation resistance, small toxic and side effects and the like, and can realize efficient ethanol metabolism regulation and anti-oxidation protection.