16 results about "Acetobacter" patented technology

This invention belongs to the field of vinegar brewing technology, and provides a multi-strain co-fermentation selenium-enriched active probiotic functional vinegar and its preparation method. Jujube enzymatic hydrolysate, malt juice, and ultrafine powder of Astragalus-Codonopsis compound traditional Chinese medicine are mixed in a certain proportion, and sodium selenite is added. Alcoholic fermentation is carried out using Saccharomyces cerevisiae CGMCC 34906. After alcoholic fermentation, acetic acid and lactic acid fermentation is carried out using a compound inoculum of Lactobacillus plantarum and Acetobacter pasteurization. After fermentation, the mixture is centrifuged, filtered, sterilized, post-ripened, blended, and sterilized. Active selenium-enriched Lactobacillus plantarum microcapsules are added, resulting in a double enhancement of selenium enrichment and active probiotics, yielding a selenium-enriched active probiotic functional vinegar. The vinegar is reddish-brown in color and has a complex aroma of jujube and malt fermentation, along with the aroma of traditional Chinese medicine. It has a mellow and pure sour taste with a long aftertaste. The flavor is rich and pleasant; total selenium ≥ 0.10 mg / L, and the number of viable lactic acid bacteria ≥ 10. 7 CFU / mL, polysaccharides ≥60.00 mg / 100 mL, saponins ≥2.50 mg / 100 mL.

The invention discloses Acetobacter peach and application thereof in prevention and treatment of postharvest diseases, and belongs to the technical field of microorganisms and the field of biological prevention and treatment of postharvest diseases. The Acetobacter persici NM-1 disclosed by the invention has an antagonistic effect on telosporium roseum and alternaria alternata, and has a remarkable prevention and treatment effect on postharvest diseases of apples such as red rot and black spot. The invention provides a biocontrol bacterium resource for prevention and treatment of apple red rot and black spot.

The invention discloses a method for enhancing the flavor of sea buckthorn fruit vinegar through synergistic fermentation of multiple strains combined with medicinal and edible raw materials. Wickerhamomyces anomalus YCJP320 with clear hereditary characteristics and proprietary intellectual property rights (CGMCC No. 33925, which can produce esterifying enzyme at high yield and increase aroma components such as ester components) and acetobacter pasteurianus CL247, CGMCC No. 33924, which can resist 18% ethanol and 7% acetic acid, and can produce acid efficiency are respectively used for alcoholic fermentation and acetic fermentation, and then fermentation is carried out, so that the fermentation efficiency is improved. According to the fermentation production process of the sea buckthorn fruit vinegar, ganoderma lucidum post-prebiotics, phellinus linteus post-prebiotics and medicinal and edible raw materials (astragalus membranaceus, dogwood and cinnamon) are used as fermentation culture medium components for nutrient enrichment, so that directional regulation and control of flavor substances and functional components of the sea buckthorn fruit vinegar are realized.

This invention discloses a strain of *Pediococcus pentosaceus* RL-10 and its application in bio-acidification rice soaking and bacterial cellulose production, belonging to the field of microbial technology. The preservation number of *Pediococcus pentosaceus* RL-10 is CCTCC NO: M 20252428. This invention is the first to use *Pediococcus pentosaceus* as a strain for bio-acidification rice soaking. Using rice soaking water as a production substrate, *Acetobacter Hansenulatus* and strain RL-10 are co-cultured in situ to produce bacterial cellulose loaded with antimicrobial peptides. Under certain process conditions, acidification of rice soaking by strain RL-10 can rapidly increase acidity and shorten soaking time. The bacterial cellulose production process of this invention is simple and low-cost; verification shows that the bacterial cellulose prepared by the method of this invention has high yield and good antibacterial activity, meeting the requirements of large-scale industrial production.

The application belongs to the technical field of bioengineering, and discloses a gene engineering bacterium of acetobacter pasteurii with high temperature resistance, a construction method and application thereof. Acetobacter pasteurianus The gene engineering bacterium is constructed by using the acetobacter pasteurii (ATCC 10137) as a starting strain and knocking out the transposase gene in the genome of the acetobacter pasteurii (ATCC 10137) by a homologous recombination method. TnsR The gene engineering bacterium with high temperature resistance is obtained. TnsR The deletion of the gene is beneficial to maintaining the stability of the genome, so that the strain can maintain better growth and metabolic capacity under high-temperature pressure. Experimental results show that the biomass, survival rate and acetic acid yield of the gene engineering strain under the condition of 40 DEG C high temperature are significantly better than those of the wild-type strain. When the gene engineering strain is applied to traditional solid-state food vinegar brewing, the yield of organic acids such as acetic acid is significantly improved, the content of ester flavor substances is greatly improved, and the flavor quality of food vinegar is effectively improved. The application provides an effective technical means for solving the problem that high temperature in summer leads to abnormal fermentation and yield reduction of food vinegar.

This invention discloses a method for preparing rum with high total ester content. The method includes the following steps: pretreating molasses; preparing a first fermentation medium using the pretreated molasses; expanding the culture of fissile yeast seed liquid in the first fermentation medium to obtain fissile yeast fermentation broth; continuously adding the pretreated molasses to the fissile yeast fermentation broth, culturing to obtain a fermentation product, wherein the residual sugar content of the fermentation product is controlled at 0.5-2%; preparing a second fermentation medium using the pretreated molasses; inoculating the fermentation bacteria into the second fermentation medium, culturing to obtain a fermentation broth; the fermentation bacteria include at least one of Clostridium acetic acid and Acetobacter pasteurella; adding the fermentation broth to the fermentation product, culturing to obtain a mash; continuously distilling the mash, using a kettle heating method for three-part distillation, mixing the second fraction with oak shavings, and aging; obtaining the base spirit, which is then blended to obtain rum with high total ester content. This method can produce mash with high alcohol content and high ester content in a short time.

The invention provides a composition containing acetobacter saxatilis and pantoea azototrophicus and application of the composition. The composition provided by the invention comprises acetobacter sativus or progeny thereof and pantoea azototrichum or progeny thereof; the registration number of the acetobacter breviscapus in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36149; the registration number of the nitrogen-fixing pantoea in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36150. The invention further discloses a preparation method of the nitrogen-fixing pantoea. The acetobacter sativus and the nitrogen-fixing pantoea or the composition of the acetobacter sativus and the nitrogen-fixing pantoea can be used for reducing emission of nitrogen-containing gas in the livestock breeding process and the livestock manure (normal state and composting) treatment process, and has a good application prospect.

The invention relates to acetobacter breviscapus GJ1-4, a microbial agent of the acetobacter breviscapus GJ1-4 and application of the acetobacter breviscapus GJ1-4 in promoting acidification of kitchen waste and biogas production, and belongs to the technical field of microorganisms The Acetobacter cereus GJ1-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32450, the preservation date is October 31, 2024, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the acetobacter breviscapus GJ1-4 in promotion of acidification and biogas production of kitchen waste. The acetobacter breviscapus GJ1-4 provided by the invention can efficiently grow in a pH range of 4.0-9.0, has the characteristics of efficient acid production, high ammonia nitrogen resistance and good culture, and can effectively promote acidification of kitchen waste and production of biogas.

The present application belongs to the field of microorganism and fermentation technology, and discloses a method for synergistically fermenting ginseng powder by bacteria and enzymes, and fructobacillus, ginseng spice and application. The method comprises the following steps: (1) preparing ginseng powder liquid medium; (2) inoculating fructobacillus and cellulase, and liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuing fermentation for 96-120 hours; (4) after the fermentation is completed, supplementing ethanol to a final volume concentration of 45%-55%, stirring at room temperature for 24 hours, standing and settling, and filtering to obtain supernatant, which is ginseng spice. Through the synergistic fermentation of bacteria and enzymes and the specific microbial fermentation metabolism control technology, the ginseng spice obtained by the present application can significantly reduce the earthy smell of traditional ginseng spice, has typical aroma characteristics, has strong absorbability, and effectively improves the product quality of ginseng spice series cigarettes.

The invention belongs to the technical field of application of microorganisms, and particularly relates to a diazo nutritional type acetobacter gluconicum mutant, a nitrogen-fixing microbial agent, a preparation method of the nitrogen-fixing microbial agent and application of the nitrogen-fixing microbial agent. The mutant strain with high nitrogenase activity and genetic stability is obtained through ARTP mutagenesis screening, the nitrogen fixation effect is remarkably improved, and the nitrogenase activity of the mutant strain on a nitrogen-free culture medium reaches 368.84 nmol.mg <-1 >. H <-1 >. The nitrogen-fixing bacterial strain disclosed by the invention can effectively improve index values such as plant height, root length and plant weight of rice, has a good growth-promoting effect on various crops, and is a broad-spectrum growth-promoting nitrogen-fixing bacterial strain. The nitrogen-fixing bacterial strain can be rapidly cultured in a short time, and is suitable for industrial production. The diazo nutritional glucose acetobacter mutant is a high-enzyme-activity mutant strain obtained through ARTP mutagenesis and acetylene reduction method screening and has good nitrogen fixation capacity, the nitrogenase activity of the diazo nutritional glucose acetobacter mutant is improved by 3.1 times, and the hereditary stability of the diazo nutritional glucose acetobacter mutant is good after multiple times of subculture.

The invention belongs to the technical field of microbial fermentation, and particularly relates to acetobacter luviangensis and application thereof in shortening the growth period of crops in a rice-wheat rotation system. The invention discloses an Acetobacter luvianense JY1053 strain, a preparation method of a microbial agent of the Acetobacter luvianense JY1053 strain, and application of the Acetobacter luvianense JY1053 strain in shortening the growth period of crops in a rice-wheat crop rotation system. The microbial agent can significantly shorten the growth periods of rice and wheat and increase the yield of rice and wheat, compared with conventional fertilization, the growth period in the rice season is shortened by 9 days, the yield is increased by 8.97%, the growth period in the wheat season is shortened by 5 days, and the yield is increased by 6.35%. In addition, the Acetobacter luwanensis JY1053 has a relatively good antagonistic effect on fusarium oxysporum cucumber specialized type, botrytis cinerea, alternaria tenuissima and fusarium solani, and the Acetobacter luwanensis JY1053 has a relatively good antagonistic effect on fusarium oxysporum cucumber specialized type, botrytis cinerea, alternaria tenuissima and fusarium solani.

This invention discloses a method for producing ethanol by fermentation of Saccharomyces cerevisiae based on a dual antimicrobial strategy. The method involves inhibiting the respiratory chain of contaminating microorganisms with cyanamide and utilizing the overexpression of the gene encoding cyanamide hydratase in Saccharomyces cerevisiae 1308. CAH To reduce the impact of cyanamide on Saccharomyces cerevisiae, this invention employs the antimicrobial peptide DptB and the endolysin LysKB317 to target and inhibit Acetobacter and Lactobacillus. This invention combines the chemical antimicrobial properties of cyanamide with the biological antimicrobial properties of the endolysin, forming a dual antimicrobial strategy in the fermentation process of Saccharomyces cerevisiae for ethanol production. The synergistic effect of chemical and biological antimicrobial action achieves an organic combination of broad-spectrum and targeted antimicrobial activity. This effectively inhibits contaminating bacteria while reducing interference with Saccharomyces cerevisiae metabolism caused by chemical antimicrobial agents, thus lowering the risk of contamination during industrial ethanol production. Ultimately, it improves the stability and economic efficiency of ethanol production, increasing the ethanol yield of the 50–200 g / L glucose fermentation system by 10.1%–23.7% compared to the control group.

This invention discloses a method for preparing a mulberry-monk fruit fermented beverage, belonging to the field of microbial fermentation technology. The method includes raw material processing, alcoholic fermentation, acetic acid fermentation, and blending steps: Monk fruit is extracted using a combination of juice extraction and hot water countercurrent extraction; the extract is kept warm to improve clarity and inactivate enzymes; using mulberry juice and monk fruit juice as substrates, fruit wine yeast is inoculated for anaerobic fermentation; then, monk fruit juice and edible alcohol culture are added to adjust the alcohol content, followed by aerobic fermentation with *Acetobacter acetosporum* and acetic acid bacteria; finally, the fermented juice, glycoside-containing monk fruit juice, mulberry concentrate, and erythritol are blended to prepare the beverage. This invention fully utilizes mulberry and monk fruit resources, retains active ingredients such as polyphenols, anthocyanins, and polysaccharides, and produces a low-calorie, flavorful, and safe product suitable for industrial production, possessing both nutritional and health benefits.

The inactivated lactic acid bacteria and the metagen thereof disclosed by the invention can be used for preparing a product for inhibiting helicobacter pylori. Besides, the pediococcus acidilactici Lab2 metagen and the acetobacter aceti AS1.41 metagen are compounded, it is found that compounding of the pediococcus acidilactici Lab2 metagen and the acetobacter aceti AS1.41 metagen is remarkably higher than that of single Lab2 metagen or acetobacter aceti metagen, and in the ethanol metabolism level, the compound metagen remarkably improves ADH and ALDH activity of the mouse liver. Secondly, on the oxidative stress regulation level, the SOD, CAT activity and GSH content of the mouse liver are remarkably improved by compounding the metabiotics, and meanwhile, the MDA level is reduced. In the aspects of liver function protection and lipid metabolism regulation, the compound metabiotics can significantly reduce the activity of ALT and AST in serum, and can significantly reduce the level of TG and TC in serum at the same time. The liver tissue pathological section further proves that the composite metagen can obviously relieve hepatocyte edema and microvesicle fatty degeneration, recover normal structural arrangement of the liver tissue and realize comprehensive protection on the alcoholic acute liver injury.

The present invention provides a composition that extends lifespan and / or reduces or delays the onset of at least one age-related symptom or condition in a subject. In some embodiments, a composition comprises at least one bacterial strain or an extract or component thereof and an excipient. In some embodiments, at least one bacterial strain comprises the genus Gluconobacter, Acetobacter, Gluconacetobacter, Acidimonas, Plurashibacter, Asia, Granobacter, Cozecia, NeoAsia, NeoKacia, Gulgarobacter, Granobacter, Cozecia, Chrysanthemum, or a combination thereof. The invention also relates to a method for preparing the bacterial strain. The method includes selecting at least one bacterial strain from the bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, selecting at least one bacterial strain, and selecting at least one bacterial strain. Methods of making and using the compositions disclosed herein are also provided.