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37 results about "Acetobacter" patented technology

Acetobacter is a genus of acetic acid bacteria. Acetic acid bacteria are characterized by the ability to convert ethanol to acetic acid in the presence of oxygen. Of these, the genus Acetobacter is distinguished by the ability to oxidize lactate and acetate into carbon dioxide and water. Bacteria of the genus Acetobacter have been isolated from industrial vinegar fermentation processes and are frequently used as fermentation starter cultures.

Microbial agent for efficiently producing acid by fermenting organic wastes and application of microbial agent

PendingCN120349923ABacteriaWaste based fuelBiotechnologyAcetobacter
The invention provides a microbial agent for producing acid through fermentation of organic waste and application of the microbial agent, and relates to the technical field of biology, the microbial agent contains Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus, and the microbial agent is prepared from the following raw materials: Acetobacter xylinum, Clostridium bovifaecis and Eubacter mucilaginosus. Studies find that acetobacter burgii, clostridium cow dung and eubacterium mucilaginosus complement each other in the anaerobic fermentation process, the degradation efficiency of organic matters such as carbohydrates and proteins in the organic wastewater / perishable garbage is improved, the yield of volatile fatty acids is remarkably increased, meanwhile, the emission of CO2 is reduced, and the environmental pollution is reduced. The method has the application prospects of organic wastewater / perishable garbage treatment and recycling. When the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the fermented mixed acid liquid is rich in VFA and can be used as an organic carbon source, and the additional value is higher; when the acid-producing bacterium agent is applied to organic wastewater / perishable garbage, the operation is simple and convenient, the cost is low, biogas slurry treatment links and cost are reduced, the added value of products can be improved, and the acid-producing bacterium agent has an engineering application prospect.
Owner:WUXI UNIV

Method for synergistically fermenting ginseng powder by bacteria and enzymes, acetobacter aceti, ginseng spice and application of acetobacter aceti and ginseng spice

The invention belongs to the technical field of microorganisms and fermentation, and discloses a method for synergistically fermenting ginseng powder through bacteria and enzymes, acetobacter aceti, ginseng spice and application, and the method comprises the following steps: (1) preparing a ginseng powder liquid culture medium; (2) inoculating acetobacter aceti and cellulase, and performing liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuously fermenting for 96-120 hours; and (4) after fermentation is finished, supplementing ethanol until the final volume concentration of ethanol is 45-55%, stirring at normal temperature for 24 hours, standing, settling and filtering to obtain supernate, namely the ginseng spice. According to the preparation method, the earthy smell of the traditional ginseng spice can be remarkably reduced by the obtained ginseng spice through technologies such as bacterium-enzyme synergy and specific microbial fermentation metabolism control, meanwhile, the ginseng spice has typical aroma characteristics and is high in suckability, and meanwhile, the product quality of ginseng spice series cigarettes is effectively improved.
Owner:TIANJIN UNIV OF SCI & TECH

Acetobacteria for use in the preparation of fermented products

ActiveDE602021049414T2BacteriaTea extractionBiotechnologyAcetobacter
Owner:COMPAGNIE GERVAIS DANONE SA

Ethanol-tolerant and acetic-tolerant acetobacter pasteurianus and application thereof in production of fruit vinegar

The invention discloses an ethanol-tolerant and acetic-tolerant acetobacter pasteurianus strain and application thereof in production of fruit vinegar. The invention provides an acetobacter pasteurianus CL247, the classification name of the acetobacter pasteurianus is Acetobacter pasteurianus, the acetobacter pasteurianus is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 21, 2025, the preservation number is CGMCC No.33924, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The acetobacter pasteurianus CL247 has the characteristics that the acid yield in 72 hours is greater than or equal to 49 g / L, and the acetobacter pasteurianus CL247 can tolerate 7% acetic acid and 18% ethanol. An efficient, tolerant and stable strain solution is provided for fruit vinegar production, the industry is promoted to be upgraded from'low-efficiency discontinuous' to'efficient continuous', and remarkable technical innovation and economic value are achieved.
Owner:SHANXI FUNCTIONAL FOOD RES INST OF SHANXI AGRI UNIV +1

Multi-strain combined fermentation of selenium-rich active probiotic functional vinegar and preparation method thereof

This invention belongs to the field of vinegar brewing technology, and provides a multi-strain co-fermentation selenium-enriched active probiotic functional vinegar and its preparation method. Jujube enzymatic hydrolysate, malt juice, and ultrafine powder of Astragalus-Codonopsis compound traditional Chinese medicine are mixed in a certain proportion, and sodium selenite is added. Alcoholic fermentation is carried out using Saccharomyces cerevisiae CGMCC 34906. After alcoholic fermentation, acetic acid and lactic acid fermentation is carried out using a compound inoculum of Lactobacillus plantarum and Acetobacter pasteurization. After fermentation, the mixture is centrifuged, filtered, sterilized, post-ripened, blended, and sterilized. Active selenium-enriched Lactobacillus plantarum microcapsules are added, resulting in a double enhancement of selenium enrichment and active probiotics, yielding a selenium-enriched active probiotic functional vinegar. The vinegar is reddish-brown in color and has a complex aroma of jujube and malt fermentation, along with the aroma of traditional Chinese medicine. It has a mellow and pure sour taste with a long aftertaste. The flavor is rich and pleasant; total selenium ≥ 0.10 mg / L, and the number of viable lactic acid bacteria ≥ 10. 7 CFU / mL, polysaccharides ≥60.00 mg / 100 mL, saponins ≥2.50 mg / 100 mL.
Owner:SHANXI FUYUAN CHANGLAO MATURE VINEGAR CO LTD

Acetobacter peach and application thereof

The invention discloses Acetobacter peach and application thereof in prevention and treatment of postharvest diseases, and belongs to the technical field of microorganisms and the field of biological prevention and treatment of postharvest diseases. The Acetobacter persici NM-1 disclosed by the invention has an antagonistic effect on telosporium roseum and alternaria alternata, and has a remarkable prevention and treatment effect on postharvest diseases of apples such as red rot and black spot. The invention provides a biocontrol bacterium resource for prevention and treatment of apple red rot and black spot.
Owner:HEBEI AGRICULTURAL UNIV.

Method for enhancing flavor of sea buckthorn fruit vinegar through synergistic fermentation of multi-strain combined medicinal and edible raw materials

The invention discloses a method for enhancing the flavor of sea buckthorn fruit vinegar through synergistic fermentation of multiple strains combined with medicinal and edible raw materials. Wickerhamomyces anomalus YCJP320 with clear hereditary characteristics and proprietary intellectual property rights (CGMCC No. 33925, which can produce esterifying enzyme at high yield and increase aroma components such as ester components) and acetobacter pasteurianus CL247, CGMCC No. 33924, which can resist 18% ethanol and 7% acetic acid, and can produce acid efficiency are respectively used for alcoholic fermentation and acetic fermentation, and then fermentation is carried out, so that the fermentation efficiency is improved. According to the fermentation production process of the sea buckthorn fruit vinegar, ganoderma lucidum post-prebiotics, phellinus linteus post-prebiotics and medicinal and edible raw materials (astragalus membranaceus, dogwood and cinnamon) are used as fermentation culture medium components for nutrient enrichment, so that directional regulation and control of flavor substances and functional components of the sea buckthorn fruit vinegar are realized.
Owner:SHANXI UNIV +1

A strain of Pediococcus pentosaceus RL-10 and its application in bio-acidification of rice soaking and production of bacterial cellulose

This invention discloses a strain of *Pediococcus pentosaceus* RL-10 and its application in bio-acidification rice soaking and bacterial cellulose production, belonging to the field of microbial technology. The preservation number of *Pediococcus pentosaceus* RL-10 is CCTCC NO: M 20252428. This invention is the first to use *Pediococcus pentosaceus* as a strain for bio-acidification rice soaking. Using rice soaking water as a production substrate, *Acetobacter Hansenulatus* and strain RL-10 are co-cultured in situ to produce bacterial cellulose loaded with antimicrobial peptides. Under certain process conditions, acidification of rice soaking by strain RL-10 can rapidly increase acidity and shorten soaking time. The bacterial cellulose production process of this invention is simple and low-cost; verification shows that the bacterial cellulose prepared by the method of this invention has high yield and good antibacterial activity, meeting the requirements of large-scale industrial production.
Owner:ZHEJIANG SHUREN UNIV

Flashing watercolor ink and preparation method thereof

PendingCN121136512AInksFluid phaseAcrylic resin
The invention belongs to the technical field of ink, and particularly relates to flash watercolor ink and a preparation method thereof. The raw materials comprise water, PEG, a dispersing agent, aluminum shimmering powder, a penetrating agent, a suspending agent, acrylic resin, a mildew preventive and dye. Wherein the suspending agent comprises at least one of a bacterial cellulose suspension, a modified bacterial cellulose suspension and microcapsule powder, the bacterial cellulose suspension is obtained by fermenting acetobacter aceti, and the modified bacterial cellulose suspension is obtained by modifying the bacterial cellulose suspension. The preparation method comprises the following steps: weighing the raw materials in parts by weight; adding water, PEG, a dispersing agent and a penetrating agent into a stirring container, and uniformly stirring to obtain a liquid phase; adding acrylic resin into the liquid phase, continuously stirring, adding a suspending agent, stirring, adding aluminum glittering powder, dye and a mildew preventive, adjusting the pH, uniformly stirring, and adjusting the viscosity to obtain the glittering watercolor ink. The ink disclosed by the invention is not easy to generate dead precipitation and mildew, and can be used for writing smoothly.
Owner:NINGBO DEYAO NEW MATERIAL CO LTD

Application of acetobacter orientalis T1-1 in preparation of glycyrrhetinic acid

The invention relates to the technical field of microorganisms, and particularly discloses application of acetobacter orientalis T1-1 in preparation of glycyrrhetinic acid. The invention discloses a high-yield strain of glycyrrhetinic acid, namely acetobacter orientalis T1-1, and the yield of the glycyrrhetinic acid can reach 1.62%-2.09% after the glycyrrhiza is fermented by the acetobacter orientalis T1-1. Compared with fermentation of a control strain, the yield of a target product can be increased by 67%-386%. On the basis, the invention provides the preparation method of the glycyrrhetinic acid, the preparation method has the advantages of short fermentation period, high target product yield, low energy consumption, mild preparation conditions and easiness in control, and can be used for green, efficient and industrial production of the glycyrrhetinic acid, and the prepared glycyrrhetinic acid can be further applied to the fields of foods, medicines, daily chemicals and the like.
Owner:HENAN AGRICULTURAL UNIVERSITY +1

A high-temperature-resistant acetobacter pasteurii genetically engineered bacterium, a construction method and application thereof

PendingCN122256213Aimprove survival rateIncrease synthesisBacteriaMicroorganism based processesBiotechnologyAcetobacter
The application belongs to the technical field of bioengineering, and discloses a gene engineering bacterium of acetobacter pasteurii with high temperature resistance, a construction method and application thereof. Acetobacter pasteurianus The gene engineering bacterium is constructed by using the acetobacter pasteurii (ATCC 10137) as a starting strain and knocking out the transposase gene in the genome of the acetobacter pasteurii (ATCC 10137) by a homologous recombination method. TnsR The gene engineering bacterium with high temperature resistance is obtained. TnsR The deletion of the gene is beneficial to maintaining the stability of the genome, so that the strain can maintain better growth and metabolic capacity under high-temperature pressure. Experimental results show that the biomass, survival rate and acetic acid yield of the gene engineering strain under the condition of 40 DEG C high temperature are significantly better than those of the wild-type strain. When the gene engineering strain is applied to traditional solid-state food vinegar brewing, the yield of organic acids such as acetic acid is significantly improved, the content of ester flavor substances is greatly improved, and the flavor quality of food vinegar is effectively improved. The application provides an effective technical means for solving the problem that high temperature in summer leads to abnormal fermentation and yield reduction of food vinegar.
Owner:JIANGSU UNIV OF SCI & TECH

Application of compound bacteria in reducing content of reducing sugar in apple juice and product

PendingCN120130603AFungiBacteriaBiotechnologyAcetobacter
The invention relates to application of compound bacteria in reducing the content of reducing sugar in apple juice and a product, and belongs to the field of food fermention.The compound bacteria are composed of a triple compound strain and a single strain, and an application method comprises first-stage fermentation and second-stage fermentation, the first-stage fermentation comprises the following steps: transferring apple juice with the sugar content mass ratio of 5-20% into a container, adding a triple composite strain, and carrying out sealed fermentation at 25-35 DEG C for 1-5 days to obtain a first-stage fermentation solution; the second-stage fermentation comprises the following steps: adding single strain acetobacter aceti into the first-stage fermentation liquor, and carrying out second-stage fermentation for 20-25 days to obtain final fermentation liquor; filtering and sterilizing to obtain low-sugar apple juice; according to the method, the mass ratio of reducing sugar in the apple juice can be reduced by 95% or above.
Owner:DONGGUAN XUJI FOODSTUFF +1

A method for preparing a rum with a high total ester content

PendingCN122302989AIncrease wine yieldHigh acid ester contentBiotechnologyAcetobacter
This invention discloses a method for preparing rum with high total ester content. The method includes the following steps: pretreating molasses; preparing a first fermentation medium using the pretreated molasses; expanding the culture of fissile yeast seed liquid in the first fermentation medium to obtain fissile yeast fermentation broth; continuously adding the pretreated molasses to the fissile yeast fermentation broth, culturing to obtain a fermentation product, wherein the residual sugar content of the fermentation product is controlled at 0.5-2%; preparing a second fermentation medium using the pretreated molasses; inoculating the fermentation bacteria into the second fermentation medium, culturing to obtain a fermentation broth; the fermentation bacteria include at least one of Clostridium acetic acid and Acetobacter pasteurella; adding the fermentation broth to the fermentation product, culturing to obtain a mash; continuously distilling the mash, using a kettle heating method for three-part distillation, mixing the second fraction with oak shavings, and aging; obtaining the base spirit, which is then blended to obtain rum with high total ester content. This method can produce mash with high alcohol content and high ester content in a short time.
Owner:INST OF BIOLOGICAL & MEDICAL ENG GUANGDONG ACAD OF SCI +3

Composition containing acetobacter saxatilis and nitrogen-fixing pantoea and application of composition

The invention provides a composition containing acetobacter saxatilis and pantoea azototrophicus and application of the composition. The composition provided by the invention comprises acetobacter sativus or progeny thereof and pantoea azototrichum or progeny thereof; the registration number of the acetobacter breviscapus in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36149; the registration number of the nitrogen-fixing pantoea in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.36150. The invention further discloses a preparation method of the nitrogen-fixing pantoea. The acetobacter sativus and the nitrogen-fixing pantoea or the composition of the acetobacter sativus and the nitrogen-fixing pantoea can be used for reducing emission of nitrogen-containing gas in the livestock breeding process and the livestock manure (normal state and composting) treatment process, and has a good application prospect.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS +1

A method for preparing fatty acids by biological hydrolysis coupling of soapstock and co-producing biogas

The present invention discloses a method for hydrolyzing and coupling soapstock by biological method to jointly produce fatty acids and biogas, which comprises the following steps: Step 1: Mix materials, control the temperature and carry out aerobic fermentation. The materials include soapstock, acetic acid bacteria, methanobacterium and water. During the acidification of soap, the hydrolysis of oil by lipase is coupled to generate crude fatty acids. The water layer rich in organic matter is discharged into fermentation tank B, and the oil layer (crude fatty acids) is discharged into tank C. Step 2: Anaerobic fermentation is carried out in fermentation tank B. After the gas production is completed, the temperature is raised to denature and inactivate the microorganisms. Then, centrifugation is carried out to separate the solid phase, oil phase and water phase. The solid phase and water phase are used for ecological organic fertilizer, and the oil phase is merged into the crude fatty acid tank C. Step 3: After the crude fatty acids are heated, dehydrated and dried, rectification is carried out to obtain the finished fatty acids. The operation of the present invention is simple, the cost is low, it is environmentally friendly, and fatty acids, biogas and ecological organic fertilizer are obtained respectively, further improving the production benefit. The production process is safe and reliable and is convenient for popularization and application.
Owner:HEFEI FEIMU BIOTECHNOLOGY CO LTD

Hermetia illucens breeding feed based on waste mushroom sticks, preparation method and hermetia illucens breeding and fecula treatment method

The invention discloses a hermetia illucens breeding feed based on waste mushroom sticks. The hermetia illucens breeding feed comprises mushroom stick residues and eutrophic balls which are uniformly dispersed, 0.2-2% by mass of a compound microbial agent is uniformly added into the mushroom stick residues; the compound microbial agent is prepared from 25 to 35 parts of bacillus subtilis, 15 to 25 parts of lactic acid bacteria, 13 to 17 parts of actinomycetes, 13 to 17 parts of saccharomycetes, 8 to 12 parts of photosynthetic bacteria and 8 to 12 parts of bacillus aceticus; the eutrophic ball comprises a ball core and a ball shell, the ball core comprises mushroom stick powder, bacillus aceticus, bacillus subtilis, a microelement additive, pregelatinized starch and carboxymethyl cellulose which are uniformly mixed, and the ball shell comprises starch and brown sugar which are uniformly mixed. The invention further discloses a preparation method of the hermetia illucens culture based on the waste mushroom sticks, a hermetia illucens culture method and a fecula treatment method. The black soldier fly breeding feed based on the waste mushroom sticks is high in biotransformation rate; the closed-loop ecological cycle of the mushroom stick residues, the insect protein and the organic fertilizer is realized.
Owner:SHANGHAI XINSHIWEI BIOTECHNOLOGY CO LTD

Calcium modified bacterial cellulose and application thereof in low-salt water animal meat paste

The invention provides calcium modified bacterial cellulose and application thereof in low-salt water livestock meat paste, and belongs to the technical field of food processing. The calcium modified bacterial cellulose is obtained by taking calcium gluconate as a carbon source and carrying out in-situ fermentation on gluconacetobacter xylinum in a liquid culture medium through an ion coordination method. According to the calcium-modified bacterial cellulose disclosed by the invention, on the premise of maintaining the flavor and texture of the water livestock meat paste product, the salt addition amount and content of the water livestock meat paste product can be reduced, and the salty taste perception and the overall quality of the water livestock meat paste can be improved. The invention provides a new strategy for developing healthier and low-salt meat paste products produced by water livestock.
Owner:FUJIAN AGRI & FORESTRY UNIV

Air pressure control system in fermentation tank for table vinegar production

ActiveCN223688314UVinegar preparationAcetobacterControl system
An air pressure control system in a fermentation tank for table vinegar production comprises an air pressure control box, an air pump is arranged at the top in the air pressure control box, an air outlet of the air pump is communicated with the top of the fermentation tank through a pipeline, a through groove is formed in the bottom of the air pressure control box, and the top of the fermentation tank is communicated with the top of a vinegar liquid storage tank through a pipeline. A vent electronic valve is arranged on a communicating pipeline between the top of the fermentation tank and the bottom of the vinegar liquid storage tank, the bottom of the fermentation tank is communicated with one end of a discharge pipe, the other end of the discharge pipe is communicated with the top of the vinegar liquid storage tank, a discharge electronic pump and a discharge electronic valve are arranged on the discharge pipe, and the discharge electronic pump is communicated with the discharge electronic valve. An electronic barometer communicated with the interior of the fermentation tank is arranged on the fermentation tank, and a control panel is arranged on the air pressure control box. The air pressure in the fermentation tank can be automatically controlled, so that the oxygen content in the fermentation tank is ensured, manual treatment is not needed, the operation is simple, and the growth efficiency and production safety of acetobacter aceti in the fermentation tank can be ensured.
Owner:HEBEI RITUO BIOTECHNOLOGY CO LTD

Acetobacter xylinum with low fusel yield and application of acetobacter xylinum

The invention discloses acetobacter Indonesii with low fusel oil yield and an application of the acetobacter Indonesii. The strain of the Acetobacter indicum X3 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is July 15, 2025, and the preservation number is CCTCC NO.M20251613. The strain of the Acetobacter indicum X3 has the advantages that the strain of the Acetobacter indicum X3 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO.M20251613; the acetobacter strain is obtained by separating and purifying mash of the rice-flavor liquor, fusel oil in the rice-flavor liquor can be reduced by 14.2%, the acetobacter seed solution and distiller's yeast of the rice-flavor liquor are prepared into mixed yeast to be applied to brewing of rice-flavor raw liquor, the total acid content in a produced liquor body reaches 0.35 g / L and is increased by 66.7% compared with that of a production control group, and the production cost is reduced. The total ester content reaches 0.35 g / L and is improved by 47.8% compared with that of a control group, and the flavor purity and the comprehensive score of a wine body are optimal through sensory evaluation.
Owner:JING BRAND

Acetobacter breviscapus GJ1-4, microbial agent thereof and application of acetobacter breviscapus GJ1-4 in promotion of acidification of kitchen waste and biogas production

The invention relates to acetobacter breviscapus GJ1-4, a microbial agent of the acetobacter breviscapus GJ1-4 and application of the acetobacter breviscapus GJ1-4 in promoting acidification of kitchen waste and biogas production, and belongs to the technical field of microorganisms The Acetobacter cereus GJ1-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32450, the preservation date is October 31, 2024, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention further provides application of the acetobacter breviscapus GJ1-4 in promotion of acidification and biogas production of kitchen waste. The acetobacter breviscapus GJ1-4 provided by the invention can efficiently grow in a pH range of 4.0-9.0, has the characteristics of efficient acid production, high ammonia nitrogen resistance and good culture, and can effectively promote acidification of kitchen waste and production of biogas.
Owner:ANXINGDA (SHANDONG) ENVIRONMENTAL PROTECTION TECH CO LTD +1

Novel acetobacter pasterianus and quick producing method of marigold vinegar using the same strain

ActiveKR103023142B1AcetobacterNucleotide
The present invention relates to a novel strain of Acetobacter pasterianus and a method for producing rapid fermented vinegar using the same. More specifically, the invention relates to an Acetobacter pasterianus S25 strain (accession number: KFCC11970P) having 16S ribosomal DNA containing the nucleotide sequence indicated by SEQ ID NO. 1, capable of rapid acetic acid fermentation, thereby shortening the acetic acid fermentation period while exhibiting excellent flavor, and a method for producing rapid vinegar with excellent flavor using the same.
Owner:재단법인 남해안권발효식품산업지원센터

Method for synergistically fermenting ginseng powder by bacteria and enzyme, acetobacter fruit, ginseng spice and application

The present application belongs to the field of microorganism and fermentation technology, and discloses a method for synergistically fermenting ginseng powder by bacteria and enzymes, and fructobacillus, ginseng spice and application. The method comprises the following steps: (1) preparing ginseng powder liquid medium; (2) inoculating fructobacillus and cellulase, and liquid fermentation for 24-36 hours; (3) inoculating amylase, and continuing fermentation for 96-120 hours; (4) after the fermentation is completed, supplementing ethanol to a final volume concentration of 45%-55%, stirring at room temperature for 24 hours, standing and settling, and filtering to obtain supernatant, which is ginseng spice. Through the synergistic fermentation of bacteria and enzymes and the specific microbial fermentation metabolism control technology, the ginseng spice obtained by the present application can significantly reduce the earthy smell of traditional ginseng spice, has typical aroma characteristics, has strong absorbability, and effectively improves the product quality of ginseng spice series cigarettes.
Owner:TIANJIN UNIV OF SCI & TECH

Diazotrophic glucose acetobacter mutant, nitrogen-fixing microbial agent, and preparation method and application of diazotrophic glucose acetobacter mutant and nitrogen-fixing microbial agent

The invention belongs to the technical field of application of microorganisms, and particularly relates to a diazo nutritional type acetobacter gluconicum mutant, a nitrogen-fixing microbial agent, a preparation method of the nitrogen-fixing microbial agent and application of the nitrogen-fixing microbial agent. The mutant strain with high nitrogenase activity and genetic stability is obtained through ARTP mutagenesis screening, the nitrogen fixation effect is remarkably improved, and the nitrogenase activity of the mutant strain on a nitrogen-free culture medium reaches 368.84 nmol.mg <-1 >. H <-1 >. The nitrogen-fixing bacterial strain disclosed by the invention can effectively improve index values such as plant height, root length and plant weight of rice, has a good growth-promoting effect on various crops, and is a broad-spectrum growth-promoting nitrogen-fixing bacterial strain. The nitrogen-fixing bacterial strain can be rapidly cultured in a short time, and is suitable for industrial production. The diazo nutritional glucose acetobacter mutant is a high-enzyme-activity mutant strain obtained through ARTP mutagenesis and acetylene reduction method screening and has good nitrogen fixation capacity, the nitrogenase activity of the diazo nutritional glucose acetobacter mutant is improved by 3.1 times, and the hereditary stability of the diazo nutritional glucose acetobacter mutant is good after multiple times of subculture.
Owner:ZHEJIANG UNIV OF TECH

Compound feed additive as well as preparation method and application thereof

ActiveCN120770472AAntipyreticAnalgesicsFeed conversion ratioSaw palmetto extract
The invention relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the invention comprises the following components in parts by weight: 1-8 parts of a saw palmetto extract, 1-5 parts of an inonotus obliquus extract, 0.5-2.5 parts of a rhizoma dioscoreae nipponicae extract, 20-30 parts of a raspberry extract, 3-5 parts of a cortex erythrinae extract, 3-5 parts of compound bacteria and 10-30 parts of a carrier, furthermore, the compound bacteria are selected from enterococcus faecium, bacillus laterosporus, acetobacter ollani, lactobacillus acidophilus and lactobacillus rhamnosus; the invention also provides an animal feed prepared from the compound feed additive. The compound feed additive provided by the invention can improve the growth performance of animals from multiple aspects of enhancing animal immunity, improving animal intestinal microbe balance, increasing the feed conversion rate and the like, so that the breeding benefits of the livestock and poultry breeding industry are improved.
Owner:BEIJING DORUN TECH

Fermented camel jerky and preparation method thereof

The invention provides fermented camel jerky and a preparation method thereof, and belongs to the technical field of jerky preparation. The preparation method of the fermentation inoculant comprises the following steps: mixing black tea leaves, cane sugar and water, boiling, cooling, removing tea leaves, adding enteromorpha polysaccharide, and inoculating compound microorganisms for fermentation; the compound microorganism is prepared from the following components in parts by weight: 1 to 3 parts of staphylococcus xylosus, 1 to 2 parts of lactobacillus plantarum, 3 to 5 parts of debaryomyces hansenii, 2 to 4 parts of pediococcus pentosaceus and 2 to 4 parts of acetobacter xylinum. The prepared zymophyte is high in microbial activity, can effectively improve the flavor of the camel meat, improve the taste of the camel meat and improve the quality of the camel meat when being used for fermenting the camel meat, and provides a new alternative meat product for people.
Owner:INNER MONGOLIA VOCATIONAL COLLEGE OF COMMERCE & TRADE

A method for the synergistic application of algae and bacteria in the treatment of livestock and poultry breeding wastewater

This application discloses a method for the synergistic application of algae and bacteria in the treatment of livestock and poultry wastewater, relating to the field of livestock and poultry wastewater treatment technology. The application method includes the following steps: wastewater pretreatment; bacterial strain compounding: Enterococcus hesperidinus, Rhodococcus roseus, Pantotheca cumulus, and Acetobacter cocoa are fermented individually, and then the single fermented bacterial strains are compounded. The mass ratio of the fermentation liquid of Enterococcus hesperidinus, Rhodococcus roseus, Pantotheca cumulus, and Acetobacter cocoa is (1-3):1:(1-3):(3-5) to obtain a compound bacterial agent; microalgae compounding: Chlorella ellipsoides and Scenedesmus are cultured individually at a light intensity of 2500-4000 lux for 5-10 days at a temperature of 25-28℃, and then the single cultured microalgae are compounded to obtain a compound microalgae agent. The technical solution provided in this application can effectively degrade various pollutants in livestock and poultry wastewater, with significant treatment effects.
Owner:GUANGDONG RUIJIE ENVIRONMENTAL PROTECTION ENG CO LTD

Matrine-bacterial cellulose gel prepared through in-situ growth, preparation method of matrine-bacterial cellulose gel, matrine-bacterial cellulose sustained-release particles and application of matrine-bacterial cellulose sustained-release particles

The invention provides a method for preparing sophocarpidine-bacterial cellulose gel through in-situ growth, which comprises the following steps: crushing fresh yellow wine vinasse by using a stirrer, adding water, boiling, cooling to 60 DEG C, then adding glucoamylase, fully and uniformly stirring, carrying out enzymolysis, and filtering by using gauze after the enzymolysis is finished to obtain vinasse enzymatic hydrolysate; adding cane sugar and black tea into water, heating to boil, cooling to room temperature, filtering, taking filtrate, adding the wall-broken hibiscus manihot flavone extract into the filtrate, and stirring for dissolving to obtain a fermentation aid; taking 200ml of vinasse enzymatic hydrolysate, adjusting the pH value to 4, then adding a fermentation auxiliary agent and sophocarpidine, inoculating acetobacter gluconoxylinum, standing and culturing for 7 days at the constant temperature of 25 DEG C, and then washing and draining to obtain the sophocarpidine-bacterial cellulose gel. Meanwhile, the invention further provides the prepared matrine-bacterial cellulose gel, sustained-release particles and application of the matrine-bacterial cellulose gel and the sustained-release particles. The problems that in the prior art, the workload is large and the cost is high due to the fact that matrine needs to be frequently applied many times are solved.
Owner:JIAXING UNIV

Composite feed additive, preparation method and application thereof

The application relates to the technical field of feed additives, and particularly discloses a compound feed additive as well as a preparation method and application thereof. The compound feed additive provided by the application comprises the following components in parts by weight: saw leaf palm extract 1-8 parts, white birch mushroom extract 1-5 parts, Rhizoma Anemarrhenae extract 0.5-2.5 parts, raspberry extract 20-30 parts, sea-tortoise skin extract 3-5 parts, compound bacteria 3-5 parts and carrier 10-30 parts; further, the compound bacteria are selected from Enterococcus faecium, Bacillus laterosporus, Acetobacter orleanensis, Lactobacillus acidophilus and Lactobacillus rhamnosus; and the application also provides animal feed prepared from the compound feed additive. The compound feed additive provided by the application can improve the growth performance of animals from aspects of enhancing the immunity of animals, improving the intestinal microorganism balance of animals, improving the feed conversion rate and the like, so as to improve the breeding benefit of livestock and poultry breeding.
Owner:BEIJING DORUN TECH

Goat feed additive based on dandelion compound preparation and preparation method thereof

The invention discloses a goat feed additive based on a dandelion compound preparation and a preparation method of the goat feed additive, and relates to the technical field of biology. The preparation method comprises the following steps: mixing dandelion powder, red caltrop root powder and negundo chastetree leaf powder, adding water, adjusting the water content to be 40-60wt%, and then performing sterilization treatment to obtain a sterilized mixture; inoculating acetobacter cacao freeze-dried powder and aspergillus usamii variant freeze-dried powder into the sterilized mixture, and carrying out solid fermentation; and after the fermentation is finished, drying and crushing the product to obtain the goat feed additive based on the dandelion compound preparation. The goat feed additive based on the dandelion compound preparation can significantly promote the growth of goats and improve the daily gain and growth efficiency of the goats; meanwhile, the oxidation resistance and the immune regulation function of the goats are enhanced, so that the overall health level of the goats is obviously improved.
Owner:XUZHOU VOCATIONAL COLLEGE OF BIOENG

Acetobacter luviangensis and application thereof in shortening growth period of crops in rice-wheat rotation system

The invention belongs to the technical field of microbial fermentation, and particularly relates to acetobacter luviangensis and application thereof in shortening the growth period of crops in a rice-wheat rotation system. The invention discloses an Acetobacter luvianense JY1053 strain, a preparation method of a microbial agent of the Acetobacter luvianense JY1053 strain, and application of the Acetobacter luvianense JY1053 strain in shortening the growth period of crops in a rice-wheat crop rotation system. The microbial agent can significantly shorten the growth periods of rice and wheat and increase the yield of rice and wheat, compared with conventional fertilization, the growth period in the rice season is shortened by 9 days, the yield is increased by 8.97%, the growth period in the wheat season is shortened by 5 days, and the yield is increased by 6.35%. In addition, the Acetobacter luwanensis JY1053 has a relatively good antagonistic effect on fusarium oxysporum cucumber specialized type, botrytis cinerea, alternaria tenuissima and fusarium solani, and the Acetobacter luwanensis JY1053 has a relatively good antagonistic effect on fusarium oxysporum cucumber specialized type, botrytis cinerea, alternaria tenuissima and fusarium solani.
Owner:HUNAN INST OF MICROBIOLOGY