114 results about "Acetobacter" patented technology

The invention relates to a citrus fruit vinegar prepared by composite strain mixed fermentation, belonging to the technical field of fruit vinegar brewage. The preparation method is characterized by comprising the following steps: 1) preparing a composite microzyme strain seed solution; 2) preparing a composite acetobacter strain seed solution; 3) preparing a glutinous rice saccharified solution; 4) preparing a citrus juice; 5) preparing a fermentation liquid; 6) carrying out alcohol fermentation; 7) carrying out acetic fermentation; and 8) aging at room temperature for 2-3 months, filtering with diatomite, and clarifying to obtain the citrus fruit vinegar finished product. By using the citrus and glutinous rice as the raw materials and adopting the fruit/grain co-brewage and composite strain mixed fermentation, the prepared citrus fruit vinegar has the advantages of abundant nutrition, favorable mouthfeel, pleasant fruital aroma and no bitterness.

The invention discloses a blueberry kombucha beverage and a preparation method thereof. Raw materials and auxiliary materials comprise: blueberry fruit juice, white sugar, lactose, common black tea, Chrysanthemum morifolium, corn syrup, honey, mixed infusion of fresh ginger, mint and licorice, purified water, angel dried yeast, lactobacillus bulgaricus, bifidobacteria, lactobacillus acidophilus, Shanghai wine 1.01 acetobacter rancens, sodium erythorbate, yeast nutrient salt and dimethyl sodium bicarbonate. The raw materials and auxiliary materials need pretreatment steps of fermenting for 3-4t with a sandwich fermentation cylinder, shearing 5-10min with a high speed shearing machine, and then fermenting for 3-4t. A quantitative fermentation technology is used, and clarifying filtration and aseptic filtration are co-used, to raise biological stability of products. By regulation, product efficacies are strengthened, and goods performances are raised. Low temperature degasification and aseptic cold-filling are preformed, to guarantee product characteristics and quality.

The invention discloses a biological deodorant. The biological deodorant is characterized by comprising the following raw materials in percentage by mass: 20%-30% of a complex microbial agent, 40%-50% of organic acid and 30%-40% of calcium superphosphate, wherein the complex microbial agent comprises saccharomyces cerevisiae, bacillus subtilis, germany lactic acid bacteria, nitrobacteria, thiobacillus thiooxidans, acetobacter oxydans and an adsorption material; and the organic acid is selected from one or more of oleic acid, linoleic acid, linolenic acid, arachidonic acid, polybasic carboxylic acid, oxalic acid, malic acid, amino acid, sulfoacid and sulfinic acid. According to the biological deodorant, the original foul gas in garbage can be quickly and effectively adsorbed, removed and covered, the work environment of a worker in a composting plant is improved, composting and stabilizing of organic garbage are accelerated, and novel foul gases such as ammonia and hydrogen sulfide generated and released in the composting process are prevented from being reduced.

The invention discloses a gluconoacetobacter sp. strain and a screening and purifying method. The screening and purifying method is characterized in that: defective fruits are used as raw materials; cycloheximide is used to suppress the growth of molds and other fungi, and plate enrichment cultivation, gradient dilution plate coating, primary screening, second screening and other methods are adopted for screening and purification to obtain the strain generating bacteria cellulose. According to the description in the ninth version of Bergey's Manual of Determinative Bacteriology, the strain is determined as a novel strain by the analysis of physiological and biochemical properties and 16 Sr DNA sequence. The invention strain obtained by the method has a wide selection range of carbon sources and nitrogen sources, a maximum yield of bacteria cellulose at a pH value of 6.5 and normal temperature, and a higher yield than that of common cellulose-generating bacteria, and has better implementation prospect in the industrial production of bacteria cellulose.

The invention relates to a preparation method of Eucommiaulmoides Oliver fermentation extract, the Eucommiaulmoides Oliver fermentation extract obtained by the method, and a use of the Eucommiaulmoides Oliver fermentation extract for the preparation of cosmetics. The preparation method includes the following steps that a step of preparation of a composite strain is carried out, in the step, the composite strain is obtained by pre-culture of Acetobacter pasteurium, Lactobacillus plantarum and Saccharomyces cerevisiae separately; a step of enzymatic hydrolysis of Eucommiaulmoides Oliver powder to prepare a fermentation matrix is carried out, in the step, leaves of Eucommiaulmoides Oliver are washed, dried, crushed into powder and mixed with water, then Eucommiaulmoides Oliver enzymatic hydrolysate after enzymatic hydrolysis is obtained by adding protease, cellulase and pectinase for enzymatic hydrolysis, and the fermentation matrix is obtained based on the Eucommiaulmoides Oliver enzymatic hydrolysate; a step of fermentation is carried out, in the step, the composite strain is inoculated into the fermentation matrix containing the Eucommiaulmoides Oliver enzymatic hydrolysate after enzymatic hydrolysis and fermented under the condition of low dissolved oxygen to obtain fermentation broth; and a step of purification is carried out, in the step, the obtained fermentation broth is centrifuged and filtered to obtain the Eucommiaulmoides Oliver fermentation extract.

The invention relates to a carbonized bacterial cellulose-cadmium sulfide composite photocatalytic material as well as a preparation method and application thereof. The photocatalytic material is prepared by using the following methods: 1) inoculating Gluconacetobacter xylinus into a culture medium A, carrying out dynamic culture for 96 hours, collecting a bacterial cellulose product, washing, drying, and further carrying out vacuum freeze drying so as to obtain bacterial cellulose aerogel; 2) dissolving a fixed amount of thiourea and cadmium sulfide into ultrapure water, adding a proper amount of the bacterial cellulose aerogel, and carrying out a hydrothermal reaction at a high temperature and high pressure; 3) carrying out high-temperature calcining on the bacterial cellulose aerogel-cadmium sulfide compound product in a tubular furnace, thereby obtaining the carbonized bacterial cellulose-cadmium sulfide composite photocatalytic material. The preparation method provided by the invention has the advantages of being simple in operation, low in cost, and the like. The obtained carbonized bacterial cellulose-cadmium sulfide composite photocatalytic material can be used as a catalyst in water hydrogen generation of photocatalysis splitting.

The fermentation method of urban domestic refuse is characterized by that the composite liquid bacterial preparation or composite solid bacterial preparation containing photosynthetic bacteria, acetobacter, microzyme and lactic acid bacteria and refuse are mixed, then undergone the processes of anaerobic fermentation and aerobic souring treatment to shorten fermentation time, prevent the production of malodorous pollutant and reduce the breeding of flies and mosquitoes.

The invention discloses a acetobacter pasteurium and its application method in producing citrus vinegars and resulting citrus vinegar products. The invention provides acetobacter pasteurium named LSYY001.2, preserved in CGMCC on 14th June, 2010, preservation number: CGMCC No.4954. Method of the invention for producing citrus vinegar comprises: selecting materials, peeling citruses, removing segment membranes, crushing, filtering, adding yeasts for alcoholic fermentation, adding LSYY001.2 for acetic acid fermentation, then filtering. The acetobacter pasteurium provided by the invention not only ferments alcohol for producing acetic acid, but simultaneously produces iso-limonin hydrolase, thus realizing removing bitter of the citrus vinegar.

The invention discloses a gene-nourishing and repairing bioorganic nutrient solution. The bioorganic nutrient solution of the invention takes gene-nutrishing Ganoderma lucidum, rice sprouts, black bean sprouts, sesame sprouts, and five viscera-nourishing, Radix Polygoni Multiflori Preparata, Polygonatum sibiricumt, acanthopanax senticosus, wolfberry fruits, Fructus alpiniae oxyphyllae, and brain-nourishing goose eggs, walnut kernels, oxide resistant haws, winter jujubes, qi replenishing and blood nourishing radix astragali, Chinese Angelica, channel dredging and collateral activating radix aucklandiae, Ligusticum chuanxiong, as well as honey and drinking water as raw materials. Under the effects of beneficial strains such as acetobacter schutzenbachii, beer yeast, Lactobacillus lactis andthe like, multiple beneficial strain groups are subjected to aerobic-anaerobic combined deep processing. The gene-nourishing and repairing bioorganic nutrient solution in the invention is suitable for all groups, and has a plurality of health care functions like virus and bacterium resistance, immunity enhancement, physical strength recuperation, fatigue resistance, sleep improvement, and age defying, etc.

The invention provides acetobacter orientalis. The preservation name of the acetobacter orientalis is Acetobacter orientalis, the acetobacter orientalis is preserved in China Center for Type Culture Collection on July 16, 2015, and the preservation serial number is CCTCC NO: M 2015455. The acetobacter orientalis has good stability, is high in metabolic capability and safety and has good medicinal value. The invention further provides a method for producing astragalus polysaccharide through the acetobacter orientalis. The content of the astragalus polysaccharide obtained through fermented culture is not lower than 30%.

Disclosed herein is a novel gluconacetobacter strain having cellulose producing activity. Specifically, the present invention relates to a novel gluconacetobacter strain producing nano-structured cellulose in a highly efficient manner. The cellulose produced by the strain, due to its superb thermodynamic properties, can be characterized as nano-structured bacterial cellulose and therefore utilized as a bio-nano-fiber. Particularly, the cellulose can be impregnated with a resin to form a cellulose-based resin which can be effectively adapted for a substrate for a liquid crystal display (LCD).

The invention discloses a preparation method of an apple enzyme functional beverage, and belongs to the technical field of food. The preparation method comprises the steps of taking apples, white granulated sugar and purified water as raw materials, inoculating a fermentation preparation for three times of fermentation in sequence, firstly inoculating saccharomyces cerevisiae with the inoculationamount of 0.1-0.2%, fermenting for 5 d, and sterilizing; inoculating Acetobacter pasteurianus with the inoculation amount of 1.2-1.8%, fermenting for 3 d, and sterilizing; finally inoculating lactobacillus plantarum with the inoculation amount of 1.2-1.8%, fermenting for 4 days, and sterilizing. Compared with the gamma-aminobutyric acid prepared by simultaneous fermentation of the three bacteria,the content of the gamma-aminobutyric acid is low, and the content of total phenols and crude polysaccharides is high. The apple enzyme functional beverage disclosed by the invention is wide in raw material source, low in price, unique in sweet and sour taste and mellow in mouthfeel; the product has stable quality, no preservative, rich metabolite and easy absorption by human body. The apple enzyme functional beverage can clear toxins in the body, clear free radicals, delay aging, resist cancer, maintain beauty and keep young, enhance memory and improve immunity.

The invention provides a method for producing astragalus polysaccharide through acetobacter orientalis. The method includes the following steps that 1, a fermentation culture medium of astragalus polysaccharide is prepared; 2, an MRS bouillon culture medium is prepared; 3, enlarged culture of cetobacter orientalis is performed; 4, a fermentation reaction is performed; 5, separation and determination are performed. The method for producing astragalus polysaccharide is economical and efficient; fermentation raw materials used in the method are low in price and easy to purchase, the fermentation process is simple and easy to control, fermentation equipment and production conditions are not specially required, equipment and production conditions of a conventional fermentation plant can be adopted for production, and industrialized mass production can be easily achieved.

The invention relates to black fungus mycelia and black garlic vinegar. The black fungus mycelia and black garlic vinegar is prepared by the following method: putting garlic with peel into an incubator, and performing fermentation at high temperature so as to obtain black garlic; peeling the black garlic, thoroughly cleaning the peeled black garlic, adding water, and grinding the cleaned black garlic with the water into black garlic serosity with a colloid mill; performing pasteurization, inoculating the activated black fungus mycelia fluid into the black garlic serosity, adding magnesium sulphate, and performing incubation in a fermenter; after black fungus mycelia grows out of the black garlic serosity, adding basic liquor of white spirit for blending so as to obtain a solution, regulating the alcoholic strength of the solution to 4-5 degrees, inoculating mixed bacteria of lactic acid bacteria and acetobacter diazotrophicus to the solution with the alcoholic strength of 4-5 degrees for fermentation, performing sealing and aging fermentation on liquor at 5-8 DEG C for 2-3 months, and performing filtration and filling so as to obtain the black fungus mycelia and black garlic vinegar. According to the black fungus mycelia and black garlic vinegar disclosed by the invention, the nutrient substances of the black funguses and the nutrient substances of the black garlic are utilized, so that the black garlic vinegar is rich in flavor substances, acid and smooth in mouth feel, and rich and mellow in vinegar fragrance; the black fungus mycelia is inoculated, so that the black fungus mycelia and black garlic vinegar is extremely high in antioxidant activity capacity, enables skins of people to be rosy and glowing, and can dredge and lubricate intestines and stomachs.

The invention discloses a microorganism modifier and a preparation method and application thereof. The microorganism modifier is prepared from: Bacillus subtilis, Saccharomyces cerevisiae, Clostridiumbutyricum, Lactobacillus plantarum, Pediococcus pentosaceus, Enterococcus faecalis, Acetobacter, Bifidobacterium, Lactobacillus, Lactobacillus acidophilus, Lactobacillus casei, Photosynthetic bacteria, Actinomycetes, Clostridium butyricum and Bacillus coagulans. According to the microorganism modifier, various bacterial groups are cooperated and interacted to produce good effects, and the microorganism modifier can be widely used in breeding of various poultry or livestock. Meanwhile, the microorganism modifier can significantly enhance the immunity of poultry or livestock, promote nutrient absorption, increase feed utilization rate, and improve meat quality; and the microorganism modifier has a good therapeutic effect on poultry or livestock that have common diseases or even serious diseases, and the economic benefits of farmers can be significantly improved.

The invention discloses a phthalocyanine catalyst for dye wastewater treatment and a preparation method thereof. According to the preparation method, silver ions are reduced and attached to a phthalocyanine-nanocellulose binary catalyst by using a microorganism. The microorganism is gluconacetobacter intermedius, acetobacter xylinum or acetobacter hansenii; phthalocyanine is sulfamic acid group metal phthalocyanine; and silver ions are provided by silver nitrate, silver acetate or silver lactate. The preparation method comprises the following steps: 1, preparing a glucose culture solution containing phthalocyanine; 2, culturing microorganisms in the culture solution; 3, preparing a mixed solution containing silver ions and glucose; 4, putting a product obtained in the step 2 in the mixed solution obtained in the step 3, and adding the microorganisms; and 5, cleaning the product obtained in the step 4 to obtain the catalyst. According to the above technical scheme, reduction of silver ions on the surface of phthalocyanine is achieved, a chemical reducing agent, a protective agent and an organic solvent do not need to be added in the process, and the method has the advantages of simple operation, mild reaction conditions, green process, low cost and the like.

The invention discloses a Castanea mollissima vinegar and a preparation technique thereof. The Castanea mollissima vinegar is prepared from Castanea mollissima, wheat, distillery yeast, sugar, Acetobacter, glutinous rice, longan, Chinese wolfberry and red date. The preparation technique comprises the following steps: raw material treatment, Castanea mollissima fermentation, wheat fermentation, glutinous rice and longan fermentation, mixed distillation, blending, aging, sterilization and the like. The Castanea mollissima vinegar has the subtle fragrance of wheat and the rich fragrance of glutinous rice, and has the effects of nourishing the stomach, strengthening the spleen, tonifying the kidney, strengthening tendons, promoting blood circulation, arresting bleeding, resisting aging and prolonging the life.

The invention discloses a preparation method of bacterial cellulose. The method comprises the following steps of: preparing a soybean residue biological fermentation culture medium by using soybean residues as a basic raw material, namely soybean residues for short, adding 10-18% of a gluconacetobacter xylinum seed solution and amygdalin into the soybean residue biological fermentation culture medium, adding 12-25 mL of a gluconacetobacter xylinum seed solution into every 100 mL of the soybean residue biological fermentation culture medium, wherein the pH is 4.8-6.0, the culture temperature is 28-35 DEG C, the culture time is 12-18 days, the use amount of amygdalin is 150-450 U/mL, and obtaining a bacterial cellulose membrane material. The invention further discloses a bacterial cellulose-chitosan composite gel skin care mask and a preparation method thereof. The mask is good in tensile strength and high in mechanical strength, has a certain inhibition effect on bacteria and fungi, and is good in biocompatibility.

The invention discloses a preparation method of a heavy metal ion adsorbent based on modified bacterial cellulose. The preparation method comprises the following steps: adding xylose gluconic acetobacter seed solution into a culture solution fermentation tank filled with sweet wine brewing biogas slurry, adding low-methoxyl pectins solution for fermenting and culturing into a front fermentation mash solution; adding the front fermentation mash solution into an airlift reactor, replenishing the low-methoxyl pectins solution, fermenting and culturing by using the airlift reactor to obtain a mature post-fermentation mash containing micelle modified bacterial cellulose; separating out the micelle modified bacterial cellulose, washing the micelle modified bacterial cellulose for a plurality of times and the immersing the micelle modified bacterial cellulose into NaOH solution, carrying out water bath till the micelle modified bacterial cellulose is semitransparent; finally washing the micelle modified bacterial cellulose with deionized water, drying the micelle modified bacterial cellulose, grinding the micelle modified bacterial cellulose to be greater than 40 meshes and taking the micelle modified bacterial cellulose as the heavy metal ion adsorbent. By adopting the preparation method, the process is simple; the prepared heavy metal ion adsorbent is non-toxic, good in biocompatibility, good in absorption effect on heavy metal ions and applicable to the food industry or the pharmaceutical industry.

The invention discloses a preparation method of edible bacterial cellulose/dietary fiber, and belongs to the technical field of food biology. The preparation method comprises the following steps that1, mixed fruits are washed cleanly and squeezed to be juice, the juice is subjected to centrifugal filtration, and pectase, cellulase and other nutrient matters are added into the filtered juice, andthe mixed serves as a fermentation culture medium; 2, gluconacetobacter xylinum is inoculated, the obtained product is placed in a 30 DEG C constant temperature culture box to be cultured for 10 d, and a bacterial cellulose membrane is formed; and 3, the bacterial cellulose membrane obtained through fermentation is cut to be blocks and stirred and soaked in normal temperature water for 3-5 times,and soaking is conducted for 30-40 min each time; and the sample is placed at the temperature of 90-95 DEG C and stirred for 20-40 min by using a sodium alginate solution with the concentration being0.15-0.3%, and then standing and soaked by using calcium lactate with the concentration being 0.15-0.3% for 20-40 min at the normal temperature. The method utilizes compound fruit juice to serve as afermentation original liquid culture medium to ferment and produce bacterial cellulose. As a novel dietary fiber, bacterial cellulose is rich in nutrient structure, sour and sweet and tasty, has the attractive fragrance and pleasant color and glossiness, supplements a mass of vitamins, mineral substances and part of constant and trace elements which are needed by the human body while promoting human digestion, makes up the defects existing in common dietary fiber, and has the good quality advantage and marketing prospects.

The invention discloses a preparation method of rice vinegar with Auricularia auricula-judae and Hericium erinaceus, comprising the steps of (1) soaking and washing Auricularia auricula-judae, washingHericium erinaceus, removing root sundries, drying, and crushing to obtain Auricularia auricula-judae powder and Hericium erinaceus powder; (2) selecting glutinous rice, washing, and crushing to obtain glutinous rice flour; (3) beating the glutinous rice flour, the Auricularia auricula-judae powder and the Hericium erinaceus powder with water to obtain raw pulp of glutinous rice, Auricularia auricula-judae and Hericium erinaceus; (4) inoculating the raw pulp of the step (3) to yeast activated in step (4) to perform alcohol fermenting to obtain alcohol-fermented mash; (5) inoculating Acetobacter to the mash of the step (4), and performing acetic acid fermenting to obtain crude rice vinegar with Auricularia auricula-judae and Hericium erinaceus; (6) purifying the crude rice vinegar with Auricularia auricula-judae and Hericium erinaceus, and sterilizing to obtain the rice vinegar with Auricularia auricula-judae and Hericium erinaceus. The rice vinegar with Auricularia auricula-judae andHericium erinaceus prepared herein has clear color and pure taste, and has a richer market.