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Acetobacter orientalis and method for producing astragalus polysaccharide through same

A technology of Acetobacter orientalis and Astragalus polysaccharide, applied in the field of microorganisms, can solve the problems of difficult purification, poor stability, complicated operation steps, etc., and achieve the effects of good medicinal value, good stability and high metabolic capacity.

Inactive Publication Date: 2016-03-23
YUZHOU TIANYUAN BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

[0004] Based on the pharmacological effects and clinical value of astragalus polysaccharides, the demand for astragalus polysaccharides in the market is increasing. However, the method of directly extracting astragalus polysaccharides from astragalus has low extraction rate, poor stability, difficult purification, and complicated operation steps. Long period and other issues

Method used

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Experimental program
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Effect test

Embodiment 1

[0045] A method for producing Astragalus polysaccharides by Acetobacter orientalis according to claim 1, comprising the following steps:

[0046] (1) Take 1ml of Qinghai-Tibet Plateau yogurt and perform a 10-fold serial dilution to 10 -6 , And then take 50ul of the diluted bacterial solution in a petri dish containing MRS agar medium, spread it evenly with a coating rod, and place it in an incubator at 37℃±1℃ for 48h~72h to obtain a single colony;

[0047] (2) Take a complete single colony and inoculate it on an 18*18 slope containing 10ml medium by streaking, and then place the test tube containing the slope medium in the incubator at 37°C±1°C for 48h~72h. Obtain the slant strain and store it in the refrigerator at 4℃ for later use;

[0048] (3) Inoculate the slant strain with short bacteria age and good growth in the shake flask culture medium, and place the shake flask culture medium in a shaker at 37°C±1°C and rotate speed 100r / min~120r / min , After 48h, get the shake flask liqui...

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Abstract

The invention provides acetobacter orientalis. The preservation name of the acetobacter orientalis is Acetobacter orientalis, the acetobacter orientalis is preserved in China Center for Type Culture Collection on July 16, 2015, and the preservation serial number is CCTCC NO: M 2015455. The acetobacter orientalis has good stability, is high in metabolic capability and safety and has good medicinal value. The invention further provides a method for producing astragalus polysaccharide through the acetobacter orientalis. The content of the astragalus polysaccharide obtained through fermented culture is not lower than 30%.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Acetobacter orientalis and a method for producing astragalus polysaccharides. Background technique [0002] Acetobacter orientalis comes from a kind of yogurt unique to the Qinghai-Tibet Plateau. The color of the colonies is milky white, and the color of the colonies turns yellow in the later stage of cultivation. It was positive, and it was short rod-shaped under the light microscope. [0003] Astragalus polysaccharide has the ability to enhance the immunity of animals, induce the production of interferon in animals, has obvious low temperature resistance, can prolong the anti-survival time of normal mice and mice with depletion, has the effect of two-way regulating blood sugar, and can reduce glucose after load. The blood sugar level of mice has a hormone-like effect, prolongs the estrus period of mice, and has a good influence on the development of mice. [0004] Based on the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/02
Inventor 郭建钊梁剑平陶蕾江红格武晓娜连战胜李春慧朱永喜董运龙柴瑞红孙美鹤
Owner YUZHOU TIANYUAN BIOTECH CO LTD
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