The invention relates to the field of bio-
fermentation, in particular to a method of producing hypocrellin pigments and polysaccharides simultaneously with solution
fermentation. In the art of producing hypocrellin pigments and polysaccharides simultaneously with solution
fermentation of the invention,
bottle-shaking culture is firstly proceeded,
convolution culture is the second step, the enlarging culture of the shaking
bottle is the third step and fermentation culture is the final step. In the step of
bottle-shaking culture, strain of the hypocrellin is utilized as parent strain,
bacteria on the inclined surface of the tube are introduced to a shaking bottle inside which
liquid medium is arranged, and the charging coefficient of the shaking bottle is 0.1 to 0.25. In the step of
convolution culture, the rotation speed of the
convolution culture is 150 to 250 turnings every minute and the culture temperature is 22 to 27 DEG C. In the third step of enlarging culture of the shaking bottle, the culture material achieved by the bottle-shaking culture is shifted to the culture medium of the enlarging culture of the shaking bottle to proceed culture, the
inoculation amount is 2-10%, the rotation speed of a rotation
bed is 150-250 turnings every minute, the culture temperature is 22 to 27 DEG C, and the culture period is 72 to 120 hours. In the final step of fermentation culture, the culture material achieved by the enlarging culture of the shaking bottle is transferred to a fermentation pot inside which fermentation culture medium is installed to be cultured, the
inoculation amount is 5-10%, and the culture temperature is 22 to 27 DEG C. The hypocrellin fermentation liquid is achieved after the fermentation is finished, and the hypocrellin pigments and polysaccharides are respectively achieved by separating and purifying the achieved fermentation liquid.