97 results about "Carcinoma cell line" patented technology

The invention provides a veratramine degradation product veratrum fluorene aldehyde 1 and derivatives thereof. The invention uses steroidal alkaloid veratramine (A) as raw material and prepares veratrum fluorene aldehyde by oxidative degradation of m-chloroperoxybenzoic acid to obtain 20 derivative compounds of the veratrum fluorene aldehyde 1 after further oxidation, reduction and condensation reaction. The 20 compounds of the invention have good inhibitory activity on cancer cells, wherein the compounds 1, 2, 5, 8, 13 and 17 have good inhibitory activity on cells of human pancreatic cancer cells BxPC-3 and SW1990, small-cell lung cancer cells NCI-H446, human colorectal cancer LOVO and the like, and the inhibitory activity of the compound veratrum fluorene aldehyde 1 is the most significant.

The invention relates to the field of organic synthesis and medicine, and discloses a preparation method for N-substituted methyl-3,5-disubstituted benzylidene base-4-piperidone and biological activity for efficiently inhibiting cell line proliferation such as leukemia, ovarian cancer, breast cancer, liver cancer and esophagus cancer. The method includes: starting from various substituted methylamine and methyl acrylate, sequentially going through Michael addition, Dieckmann condensation, acidolysis and decarboxylation to obtain N-substituted methyl-4-piperidone, and subjecting the N-substituted methyl-4-piperidone to aldol reaction with substituted benzaldehyde to obtain a target compound N-substituted methyl-3,5-disubstituted benzylidene base-4-piperidone. The target compound can selectively and efficiently inhibit cell line proliferation such as leukemia, ovarian cancer, breast cancer, liver cancer and esophagus cancer, and activity of inhibiting carcinoma cell line proliferation is obviously higher than conventional chemotherapeutic 5-fluorouracil.

The invention provides a method for detecting endometrial receptivity through MST1 and phosphorylated MST1. The method comprises the steps that a human endometrial carcinoma cell line Ishikawa and HEK293T cells are kept and cultured in a 10% FBS and mycillin containing double-antibody DMEM/F12 culture solution; an immunoblotting experiment is carried out; co-immunoprecipitation is carried out; immunohistochemistry is carried out; adjustment of MST1 on the transcriptional activity of HOXA10 is analyzed through chromosome co-immunoprecipitation (ChIP)/PCRs; human choriocarcinoma cell (BeWo) sphere adhering experiments are carried out. According to the method, the expression level of MST1 and MST1 phosphorylation modification in endometrial tissue is detected conveniently and fast, and the receptivity state of the endometrium can be directly reflected.

The present invention relates to a pharmaceutical composition for the prevention and treatment of cancer comprising arazyme as an active ingredient. More precisely, when arazyme produced by Aranicola proteolycius was administered to the nude mice transplanted with human lung carcinoma cell line, weight gaining, inhibition of tumor cell growth and infiltration, and down-regulations of MMP-9, NF-κB and PCNA, were observed. In addition, when arazyme was treated to human breast cancer cells (MDA-MB-231), down regulations of p21, PCNA (Proliferating Cell Nuclear Antigen), VEGF (Vascular Endothelial Growth Factor), BCl2 (B-cell CLL/lymphoma 2), p-p38, PKC (Protein Kinase C) and MMP-1 (Matrix MetalloProteinase-1) which are involved in tumor cell growth, differentiation, proliferation and metastasis, were observed along with the up-regulation of catalase which inhibits tumor development. Therefore, the arazyme can be effectively used as a pharmaceutical composition for the prevention and treatment of cancer.

The invention provides a 2-aminoimidazopyridine derivative shown as a formula I, a formula II, a formula III or a formula IV and further provides a preparation method and application of the 2-aminoimidazopyridine derivative. An experiment shows that the 2-aminoimidazopyridine derivative has a remarkable proliferation inhibition effect on tumor cells (including an over-expressed wild type EGFR (Epidermal Growth Factor Receptor) human epidermal carcinoma cell line A431 and a Gefitinib drug-resisting human lung adenocarcinoma cell line H1975) related to the activity of EGFR tyrosine kinase in the aspect of a cell level, especially has a relatively good inhibition effect on the drug-resisting cell line H1975, has relatively weak inhibition activity on a low-expression EGFR human colon cancer cell line SW620 and can be applied to preparation of corresponding anti-tumor cell medicines. A general formula is shown in the description.

The invention provides 2-arylamine pyrimidine derivatives containing hydroxamic acid fragments shown in the formulas I and II. 2-arylamine pyrimidine containing carboxyl fragments is mainly used as a parent nucleus and is subjected to single-step condensation and related modification with hydroxylamine protected by THP to obtain a target compound. An experiment proves that the derivatives has the remarkable anti-proliferative effect on tumor cells (an overexpression EGFR human epidermal carcinoma cell line A431 and a human pulmonary carcinoma cell line H1975 resisting Gefitinib) related to EGFR tyrosine kinase activity on the cellular level, and tumor cells (a human cervical carcinoma cell line Hela, a human hepatoma cell line HepG2, a human promyelocytic acute leukemia cell line HL60, a human oral epidermoid carcinoma cell line KB, a human colon cancer cell line SW620) related to the HDAC histone acetylase activity, and the corresponding medicine for resisting cancer cells can be prepared. The general structural formula is shown in the description.

The invention provides a method for establishing a human nasopharyngeal carcinoma tumor stem cell line. The method comprises the following steps: (1) obtaining human nasopharyngeal carcinoma cells in the single cell state; (2) adding the human nasopharyngeal carcinoma cells in the single cell state, obtained in the step (1), to a serum-free stem cell culture medium containing epidermal growth factors and basic fibroblast growth factors to obtain single cell suspension; (3) adjusting the concentration of the single cell suspension in the step (2) to 900-1100 single cells/ml; (4) inoculating 1ml of the single cell suspension obtained in the step (3) into single pores on a cell culture six-pore plate and then adding the equivoluminal stem cell culture medium for culture for 72-96 hours; and (5) collecting the cells obtained in the step (4), carrying out trypsinization till the single cell and carrying out subculture. The method provided by the invention is simple and convenient to operate; and the obtained nasopharyngeal carcinoma stem cell line has obvious stem cell characteristics.

The invention discloses a human lung adenocarcinoma cell line and an application thereof. The human lung adenocarcinoma cell line is preserved at China Center for Type Culture Collection (CCTCC) on May 23th, 2014, in Wuhan University 430072, China. The name of a culture is human lung adenocarcinoma cell ZRLC-1; and a preservation number is CCTCC NO: C2014103. The human lung adenocarcinoma cell ZRLC-1 can be used as an experimental material in lung adenocarcinoma basic and clinical research, and can be used for establishing an animal model for occurrence, development and metastasis of lung cancers. The human lung adenocarcinoma cell line has stable characters, can be sub-cultured for a plurality of times stably, and can highly express epithelial markers such as EpCAM and E-cadherin. Flow cytometric analysis shows that EpCAM positive cells reach over 99.70%; and novel experiment material closer to the biological characteristics of clinical tumors are provided for the research of the lung cancers.

The invention provides a B-norcholestane benzimidazole compound as well as a preparation method and application thereof. The general structural formula of the compound is as shown in the specification, wherein R refers to any one of H or COCH3; R1 refers to F, NO2, CF3, H, CH3 or OCH3; and R2 refers to H or CH3. The B-norcholestane benzimidazole compound disclosed by the invention has high inhibitory activity on human cervical carcinoma cell lines, human lung carcinoma cell lines and human liver cancer cells. The B-norcholestane benzimidazole compound disclosed by the invention is low in toxicity and low in injury of normal cells, has an effective effect of inhibiting the cancer cells during low concentration, basically does not cause drug injury to normal cells and can be applied to preparing medicines for treating the cancers.

The invention provides 2, 3, 5, 7-tetrasubstituted dihydro-pyrazolo piperidine derivative and a preparation method and application thereof. The derivative is 2, 3-bis(substituted phenyl)-5-subsituted arylmethyl-7-substituted benzylidene dihydro-pyrazolo piperidine derivative, having the following formula (I). The preparation method includes using substituted arylmethyl amine and methyl acrylate as raw materials; subjecting the materials to Michael addition, Dieckmann condensation and hydrolysis-decarboxylation sequentially; allowing for Aldol reaction with substituted benzaldehyde to obtain intermediate N-substituted arylmethyl-3, 5-bis(substituted benzylidene)-4-piperidone; allowing for condensation with substituted phenylhydrazine to obtain a compound according to the formula (I). The derivative is efficient in inhibiting multiplication of various carcinoma cell lines such as leukemia, esophagus cancer, ovarian cancer and breast cancer in human, is well stably metabolic in liver microsomes of human and rat, is free of direct and competitive inhibition on five enzymes of liver microsomes, such as CYP3A4, CYP2D6, CYP2C9, CYP1A2 and CYP2C19, is highly bioavailable, is low in toxicity to normal cells, and is available for the preparation of drugs for the cancers.

The invention discloses a human esophageal squamous carcinoma cell line NCCE1 as well as an establishment method and application thereof. The esophageal cancer cell line disclosed by the invention ispreserved in China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: C2020166. The human esophageal squamous carcinoma cell line established by the invention is stable in character, can be stably subcultured, and can induce subcutaneous tumor formation of mice. As a cell and animal model, the model can be directly applied to research and development of anti-cancer drugs and diagnostic reagents, and provides a new experimental material closer to clinical tumor biological characteristics for esophageal squamous cell carcinoma research.

The invention relates to a 2'-pyrazol-1H-imidazole [4,5-f][1,10] phenanthroline derivate and a preparing method and application thereof. According to the 2'-pyrazol-1H-imidazole[4,5-f][1,10] phenanthroline derivate, different substituted acetophenone and semicarbazlde hydrochloride and 2,4-dinitrobenzene serve as raw materials, the target compound, namely the 2'-pyrazol-1H-imidazole [4,5-f][1,10] phenanthroline derivate is obtained through a five-step reaction, and the obtained target compound is used for applied research of cell line growth inhibition and apoptosis activity for resisting three kinds of cancer cells including the non-small cell lung cancer cell lines A549, human liver cancer cell lines HepG2 and human breast carcinoma cell lines MCF-5. Most compounds can make the experiment cancer cells restrained and apoptotic, high effects are generated on the A549 and HepG2 cell lines are achieved particularly, and selective toxicity on the A549 cell lines is generated particularly. According to the 2'-pyrazol-1H-imidazole [4,5-f][1,10] phenanthroline derivate, a large reference value is provided for finding new anti-tumor micromolecular medicines and is particularly provided for promoting clinical medicine research and development of the phenanthroline derivate.

The invention discloses a human esophageal carcinoma cell line and application thereof. The human esophageal carcinoma cell line is preserved in China Center For Type Culture Collection with a preservation number of CCTCC NO:C201661. Under the premise of retaining the main clinical biological features, the human esophageal carcinoma cell line has the characteristics of high tumor formation rate, short incubation period, good homogeneity and the like, enriches the human esophageal carcinoma cell library, provides powerful scientific research material for study of China population genetic background, and also provides a new experimental material for new drug preclinical study of in vivo and in vitro experiments.

The invention discloses a method for establishing a chicken liver injury cell model, which comprises the following steps: 1) preparing a DMEM/F12 culture medium containing fetal calf serum with the final concentration of 10%, the culture medium containing penicillin and streptomycin; 2) dissolving aflatoxin B1 in a dimethyl sulfoxide solution and diluting the aflatoxin B1 in a dimethyl sulfoxide solution with the culture medium in the step 1), obtaining an induction solution, wherein the final concentration of aflatoxin B1 in the induction solution is 0.05-0.25 microgram/mL, and the final concentration of dimethyl sulfoxide in the induction solution is 0.01-0.025%; 3) culturing the chicken liver cancer cell line until the growth vigor is good and the state is stable; and 4) when the chicken hepatoma carcinoma cell line grows to 70-80% of the adherence, adding the induction liquid in the step 2), and performing induction culture for 6-24 hours to obtain the chicken hepatoma carcinoma cell line.

The invention belongs to the field of microbe animal cell lines, and particularly relates to human biliary tract cancer cell lines and application. The human biliary tract cancer cell lines comprise human biliary tract cancer cell lines which are called a human gallbladder carcinoma cell line ZIJ-0430, a human hilar cholangiocarcinoma cell line ZJU-0826 and a human hilar cholangiocarcinoma cell line ZJU-1125 respectively, and the three human biliary tract cancer cell lines are conserved in the China Typical Culture Conservation Center with the conservation number of CCTCC NO: C2017174, CCTCC NO:C2017175 and CCTCC NO:C2017176 respectively. The human biliary tract cancer cell lines ZJU-0430, ZJU-0826 and ZJU-1125 are built from the source of Chinese people, the line building time is short, the biological inheritance trait is stable, and as study models, the biliary tract cancer cell lines provide great help for knowing a primary biliary tract cancer pathogenesis of Chinese people.

The invention discloses a method for realizing chemosensitivity influence on laryngeal carcinoma cell lines by iron-carbon nanoparticle mediated cisplatin. The method comprises preparation of iron-carbon nanoparticles, loading of cisplatin by the iron-carbon nanoparticles as well as influence of iron-carbon nanoparticle mediated cisplatin on laryngeal carcinoma Hep-2 cell microstructure, proliferation activity as well as Survivin mRNA (messenger ribonucleic acid) and caspase3 mRNA expression. According to the invention, the magnetic nano material is adopted for the first time to load cisplatin, a chemotherapeutic drug with higher magnetism and targeting is provided, the preoperative chemotherapeutic effect on laryngeal carcinoma can be improved after the drug is applied to clinical practice, the necessary and efficient chemotherapeutic drug is provided for functional laryngeal carcinoma radical treatment is provided, and the drug can be applied to postoperative chemotherapy simultaneously; and toxic and side effects of the chemotherapeutic drug are reduced, the iron-carbon nano material has high drug loading capacity, stable release rate and higher targeting, and the iron-carbon nanoparticle mediated cisplatin can play an important role in clinical popularization and application for laryngeal carcinoma treatment.

The invention relates to a high metastasis human breast carcinoma cell subline MDA-MB-231sci, which has biological and pathological features and special metastasis phenotypes. The biological features are as follows: the MDA-MB-231sci cells are typical polygonal epithelioid cells, have rich cytoplasm and big and round karyon, grow adherent to the wall with almost uniform size and are closely arranged with clear boundary. The pathological features are as follows: a tumor cell of a nude mouse in vivo metastasis part has the similar structure with the subcutaneous tumor cell, short metastasis time and high metastasis rate, wherein the metastasis time is shortened to 26-42 days; a lung metastasis rate is 100%, meanwhile, a part of axillary lymph node metastasis of an animal is performed. An establishing method for the high metastasis human breast carcinoma cell line comprises the following steps of: (1) establishing a human breast carcinoma orthotopic transplantation tumor model; (2) sieving an orthotopic transplantation lung targeted metastasis model; and (3) establishing and passaging a human breast and lung carcinomas targeted high metastasis cell line. In the invention, the success rate is improved by sieving surgical excision tumor of each generation in vivo and corresponding breast.