Colorectal carcinoma cell line CJF from hepatic metastasis and construction method thereof

A cancer cell and liver metastasis technology, applied in the direction of microorganism-based methods, tumor/cancer cells, animal cells, etc., can solve the problems that hinder the research of colorectal cancer stem cells, and achieve the effect of increasing the possibility of immortality and improving the tumor formation rate

Inactive Publication Date: 2011-03-30
ZHEJIANG UNIV
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Problems solved by technology

[0004] However, since the discovery of colorectal cancer stem cells, a good research platform for cancer stem cells has not been established. Research on the mechanism of cancer recurrence and metastasis

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  • Colorectal carcinoma cell line CJF from hepatic metastasis and construction method thereof
  • Colorectal carcinoma cell line CJF from hepatic metastasis and construction method thereof
  • Colorectal carcinoma cell line CJF from hepatic metastasis and construction method thereof

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Embodiment Construction

[0025] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0026] 1. Materials

[0027] 1. Preparation method of cell culture medium

[0028] 1.1 culture fluid RPMI1640 (I) is composed as follows: 10% (v / v) fetal calf serum (Hangzhou Sijiqing Company), 4ug / ml insulin, 100U / ml penicillin, 100ug / ml streptomycin, solvent is RPMI1640 (1: 1) Culture medium (GIBCO, USA);

[0029] Culture medium RPMI1640 (II) composition is as follows: 20ng / ml B27 (US SIGMA company), 20ng / mlEGF (US SIGMA company), 10ng / ml bEGF (US SIGMA company), 4ug / ml insulin, 100u / ml penicillin, 100ug / ml ml streptomycin, the solvent is RPMI1640 (1:1) medium (GIBCO, USA).

[0030] 1.2 Digestion solution: RPMI1640 medium containing 0.1% (w / v) collagenase and 0.1% (w / v) hyaluronidase.

[0031] 2. Other cell culture materials

[0032] Cell sieve; trypsin digestion solution: 0.25% (w / v) trypsin + 0.02% (w / ...

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Abstract

The invention provides a colorectal carcinoma cell line CJF from hepatic metastasis and a construction method thereof. The obtaining method of the colorectal carcinoma cell line comprises the following steps: obtaining tissues of colorectal carcinoma hepatic metastasis; after digesting the tissues into single cells by using collagenase / hyaluronidase, separating and inoculating CD133+ cells under NOD (Non-Obese Diabetic) / SCID rat skin by MACS (magnetic-activated cell separation); nodulating, and taking out tumor tissues; and culturing in vitro to obtain the colorectal carcinoma cell line CJF which can stably passage and is rich in colorectal carcinoma stem cells. In the invention, CJF cells are obtained from hepatic metastasis of colonic carcinoma patients; compared with other colorectal carcinoma cell lines, the colorectal carcinoma cell line CJF has incomparable advantages for the study of the colonic carcinoma hepatic metastases; cell subsets of the CJF cells for stably expressing the CD133+ cells is about 10 percent; and the action and the research of the colorectal carcinoma cell line in a colorectal carcinoma hepatic metastases process and the tumor stem cells in a metastases mechanism have wide application prospects.

Description

(1) Technical field [0001] The invention relates to a colorectal cancer cell line CJF derived from liver metastases and a construction method thereof. (2) Background technology [0002] Colorectal cancer, including colon cancer and rectal cancer, is one of the common malignant tumors. In developed countries, its incidence rate ranks third among malignant tumors, and its mortality rate ranks second. The incidence of colorectal cancer ranks third in my country and is on the rise, and the mortality rate ranks fifth in China. Invasion and metastasis are the main causes of treatment failure and death in patients with colorectal cancer, and the most common site of metastasis is the liver. Therefore, the mechanism of liver metastasis of colorectal cancer is an important topic in current tumor research. In recent years, it is believed that cancer stem cells are a type of undifferentiated cell population with self-renewal and multi-directional differentiation potential. Studies have...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12R1/91
Inventor 黄建王一刻朱永良王科
Owner ZHEJIANG UNIV
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