62results about How to "Not easy to survive" patented technology

The invention discloses a bifidobacterium longum microcapsule and a preparation method thereof. The core material is bifidobacterium longum BBMN68; the wall material comprises metaprotein and polysaccharide; the core material is mixed with the sterilized wall material; the freeze-drying bifidobacterium longum microcapsule is prepared by orifice-coagulating bath and vacuum freeze drying; the bifidobacterium amount of the microcapsule is 108 to 1011 CFU/g. After the microcapsule is stored at room temperature for 6 months, the amount of the bifidobacterium is still more than 108 CFU/g; major viable count in intestine can be realized after the freeze-drying microcapsule particles get through the stomach, and most of the bifidobacteriums are released in the intestine. The bifidobacterium microcapsule is processed by freeze drying so as to provide low-temperature, vacuum and dry environment for bifidobacterium BBMN68; thus, the microcapsule overcomes the shortcomings that bifidobacterium does not resist acid and oxidation, and is not easy to survive, and the like; moreover, the microcapsule has the advantages of long expiration date, good storage stability and enteric solubility.

The present invention discloses a cultivation method for Hainan huanghua pear nursery stock. The cuttage comprises the following steps of: carrying out scion cuttage from April to May, or September to November; shearing the base of the cuttage scion, at an angle of 30 DEG with a length direction of the cuttage scion, to form a bevel cut by use of a pair of sterile scissors before cuttage, and then vertically fixing the base of the cuttage scion in a container with a root promoting liquid to soak for 3-6 h; using a cuttage container cup to fill a nutrient medium to carry out cuttage, wherein a first-layer medium is formed by mixing yellow soil, biogas, and peat in a mass ratio of 1:1:1; a second-layer medium is formed by mixing red loam, coconut dust and vermicompost in a mass ratio of 3:1:2 ; and a third-layer medium is formed by mixing red loam and vermicompost in a mass ratio of 2:1; inserting the part, with a distance of 5 cm above the base of the cuttage scion, into the nutrient medium, and inserting the bevel cut into the second-layer medium. The cultivation method for Hainan huanghua pear nursery stock provided by the invention ensures a high survival rate of Hainan huanghua pear nursery stock , and the cutttage scion has strong adaptability to season.

The invention discloses a sweet potato virus-free breeding method, which comprises the original-original spice seedling breeding, particularly comprises steps of washing a virus-free bottle sprout and adding a medium, then colonizing the sprout on a water planting colonization plate; covering a transparent water-retaining film; and culturing the sprout with tap water; adding 1/2 of a first nutrient solution for culturing continuously when the sprout grows new white root; cutting off the shoot tip at the upper portion of the seedling when the seedling grows long for original-original specie production; supplementing 1/2 of the first nutrient solution, and cutting off the shoot tip at the upper portion of the lateral bud when the lateral bud grows long for original-original spice production. <{EN2}>According to the method, the tissue culture plants are bred by water planting first, and the survival rate can be up to 100%; the sprouts can be cut once every 7 days; the sprouts can be cut by 5-6 times in one colonization; 1 sprout can breed about 20 sprouts within two months; the factory production of the virus-free sprout can be realized completely to realize vigorous seedling and rapid propagation.

The invention discloses an air layering seedling culture method of a red maple, and belongs to a seedling culture method of the ornamental red maple. The seedling culture method includes the following steps of peeling, mud ball packing, red maple plant nutrient and water management enhancing and field planting. A rooted branch which is directly separated from a red maple plant in an air layering mode is used as a seedling for planting, it is avoided that the seedling does not easily survive due to the fact the diameter of the seedling is too small, the large self-rooting seedling with the diameter being about 4.5 centimeters and the height being about 2 meters can be cultured within a short time, and the growth rate of the seedling and trunk forms are increased by effectively managing the seedling field planting process. The seedling can be rooted and bred on the branch to be clipped from the red maple plant, and therefore waste is utilized to culture the seedling, and seedling culture cost of the red maple is lowered. The steps of the air layering seedling culture method of the red maple are simple, the seedling of the red maple can be cultured on various occasions, and the application range is wide.

The invention provides an aqueous paint composition having good coated film performance such as water resistance, alkali stability, abrasion resistance, weather resistance and the like after a biomassmaterial with the biodegradability is used. The aqueous paint composition contains (A) an aqueous resin containing carboxyl, (B) a modified starch containing carboxyl and (C) a curing agent with a functional grouping the cure reaction with carboxyl.

The invention discloses a white chrysanthemum cultivating method in the technical field of cultivation of Chinese medicinal plants. The method comprises the following steps of weeding a seedling field; selecting chrysanthemum seedlings; breeding the seedlings to strengthen the seedlings, i.e., applying manure and plant ash into the seedling field according to the proportion that 100 to 300kg of the manure and 50 to 150 kg of the plant ash are applied to every mu of seedling field; trenching a planting field; digging holes, i.e., digging the holes on the middle line of a trench surface; transplanting, i.e., transplanting the chrysanthemum seedlings which are cultured through field planting into the holes, wherein two seedlings are planted in each hole; layering, i.e., layering for the first time: after the chrysanthemum seedlings grow to the height of 30 to 50cm, applying an organic fertilizer to the two sides of each row of chrysanthemum seedlings, wherein the dosage of the organic fertilizer is 1,000kg/mu, during layering, respectively separating branches to the two sides, enabling the branches to fall d to the ground, and putting soil onto the branches every 8 to 12cm; layering for the second time: when the length of the newly grown branches is 18 to 22cm, pushing down the branches in the direction where the branches are sparse, putting the soil onto the branches, and finishing layering for the second time before the end of July; pinching, and the like. The method is high in systematic degree, and fine in data and management program; the yield of the cultivated white chrysanthemum is high, so that the economic benefit is increased.

The invention provides an iPSC residue detection method using ESRG gene as a universal marker gene. According to the iPSC residue detection method using the ESRG gene as the universal marker gene, thenumber of the ESRG genes in a sample is quantitatively detected through a qPCR detection method, so that iPSC residues in the sample are detected. According to the method, an ESRG gene segment is adopted as the universal marker gene, a series of cells including endothelial progenitor cells, neural stem cells, retinal pigment epitheliums, hepatocytes, natural killer cells, myocardial cells, isletbodies and the like can be detected, and the marker gene does not need to be replaced; and meanwhile, detection can be carried out in a short time, and the detection efficiency is greatly improved. According to the method, during detection, only mRNA extraction, reverse transcription and qPCR reagents are needed, the cost is low, and the accuracy is high.

The invention provides a potato stem tip detoxication method, which comprises the following steps of A1, selecting healthy plants for field marking; selecting big and healthy seed potatoes with standard potato shapes; breaking the dormancy of the tubers of the seed potatoes; then, performing heat treatment for 6 weeks; spraying salicylic acid twice in the period; A2, peeling big sprouts from the potato blocks obtained in the step A1; performing cleaning; A3, under the sterile conditions, cutting 1 to 3mm of the top of the big sprouts; inoculating a liquid culture medium; culturing the big sprouts into test-tube seedlings. The method has the advantages that the stem tip peeling step is simplified; the stem tip peeling technology difficulty is obviously reduced, so that the regenerated plants become strong seedlings from weak seedlings; the conventional multi-step seedling formation is changed into one-step seedling forming; the seedling forming time is about 30 days; the virus elimination period is shortened by about 33.3 percent to 60 percent; the crossing of long period to short period from the potato blocks to virus-free seedlings is realized; the seedling forming rate is increased by 44 percent or more; the current condition of stem tip peeling seedling forming difficulty is changed; the virus elimination rate is improved by 36 percent or higher; the technical bottleneck ofdifficult elimination of PVS and PVM by a conventional potato virus elimination method is broken.

The invention discloses a cryopreservation method for adherent culture of cells. The method comprises directly adding refrigerating fluid in a cell culture bottle, then cooling the culture bottle step by step, finally placing the culture bottle in an ultra-low-temperature refrigerator to store the culture bottle, storing the culture bottle for 2-3 months, unfreezing the cells when culture conditions permit, and continuing culture. Freezing and unfreezing steps are simple and rapid, the steps of cell dissociation and centrifugation and the like in a traditional freezing method are omitted, cell pollution and damage to cell viability are greatly reduced, the influence of adherence degree of the cells is avoided during freezing, the adherence degree of the cells can be 20-30%, limitation of traditional freezing to the number of the cells is avoided, and the cryopreservation method is extremely suitable for dealing with destructive damage which sudden conditions in a laboratory may cause to cell culture. A cell cryopreservation pipe does not need purchasing, the original culture bottle is directly utilized to carry out freezing, the cells do not need to be inoculated to a new culture bottle after unfreezing, culture fluid is added to the original culture bottle to continue culture, and the testing expenses are greatly reduced.

The invention discloses a shaping method for woody plants capable of serving as a chair frame. The method includes the steps: bending and shaping, and laying a seat board and a back board on two shaped plants so as to form a living plant in the shape of a chair. The specific bending method includes: smearing a cutter and a plant stem should be bent with a bactericide, then splitting the plant stem should be bent into a plurality of parts longitudinally by the cutter, rotating the stem by a rotation angle of 45-90 degrees while bending, performing angular fixation and windproof fixation, and wrapping with plastic films for preventing dehydration, wherein the width of each part is 1-2cm, and the length of each part is 2-3 three times of the diameter of the stem. By the method, diversity of plant shapes is improved, landscape content is enriched, a person can enjoy the cool on the chair due to the fact that the canopy shields from sunlight, and the defect that the chair in the open air is over-high in temperature during direct sunlight in summer is avoided.

The invention relates to the field of microorganism animal cells, in particular to a liver cancer cell system from liver cancer portal vein tumor thrombus of human and a system establishing method thereof. The invention adopts the following technical scheme that the liver cancer cell system from liver cancer portal vein tumor thrombus of human is preserved in the China Center for Type Culture Collection (CCTCC) and has a preservation number of CCTCCNO: C200814. The system establishing method of the liver cancer cell system from liver cancer portal vein tumor thrombus of human comprises the following steps: (1) acquiring cancer embolus cells and carrying out a primary culture on the embolus cells; (2) carrying out a clone expansion culture on a tumor cell island; and (3) carrying out a continuous culture on tumor cells. The invention has the advantages that CSQT-1 cells are obtained from the portal vein tumor embolus and have incomparable advantages on the forming mechanism and the tendency growing mechanism of the portal vein tumor embolus than other existing liver cancer systems. The cell system has wide prospect of application, is a sub-hypotetraploid displayed by the karyotype analysis and accounts for 58 percent of the total.

The invention discloses an unmanned boat intelligent fishery fry placement device. The device includes an unmanned boat, wherein the deck of the unmanned boat is provided with a fry placement device,the fry placement device includes a fry box fixing seat arranged on the deck of the unmanned boat, the fry box fixing seat is in sliding connection with a fry box, the fry box can slide along the length direction of the fry box fixing seat, the fry box is provided with at least two fry rooms arranged along the length direction, top walls of the fry rooms are of opening structures, two side walls of the fry rooms along the width direction are symmetrically provided with door grooves, and the door grooves are provided with box doors capable of sliding up and down. The invention further disclosesa method utilizing the fry placement device for fry placement. The device is advantaged in that the structure is simple, design is reasonable, different types of fries can be placed according to placement requirements of different fry placement zones, and fry placement varieties are enhanced; the operation method is simple, unmanned fry placement is achieved, fry placement efficiency is improved,labor and time saving is realized, and a problem of low fry placement efficiency of a traditional method is solved.

The invention discloses a salmonella strain used for RNA interference in mammalian cells. The salmonella strain is a salmonella mutant strain containing purI, msbB, phoP and phoQ gene knockout. In addition, the invention further discloses a preparation method and application of the salmonella strain.

The invention discloses a plane no-shift greening building block which comprises a block body with a square section. The block body is divided into an upper layer and a lower layer, wherein the upper layer is a cement layer, and the lower layer is an anti-skidding layer. A through hole is formed in the center of the block body, semi-circular gaps are formed in the middle portions of the four side surfaces of the block body, and one-fourth circular gaps are formed in the fourth corners of the block body. Among the fourth side surfaces of the block body, protruding blocks connected in a locking mode are arranged on two adjacent side surfaces, grooves matched with the protruding blocks are arranged on corresponding portions of the other two side surfaces, and the through hole, the semi-circular gaps and the one-fourth circular gaps are filled with grass seed layers. According to the invention, a plurality of the blocks can be spliced mutually, shift hardly occurs, a green area is large, and grass seeds in the grass seed layers easily survive. The plane no-shift greening building block has a simple structure and is convenient and practical to use.

The invention relates to a seedling raising method for miracle fruits through layering. The method comprises the following steps: (1) preparing a base material; (2) pre-treating branches used for layering; (3) covering the branches used for the layering with the base material; (4) performing fertilizer and water management; (5) performing planting in the ground; and (6) fixing roots. Compared withthe prior art, the method provided by the invention has the beneficial effects that rooted branches are directly separated from miracle fruit plants through a layering manner to be taken as saplingsfor planting, seedlings are prevented from being too small in diameter and accordingly not easy to survive, and self-rooted large seedlings can be cultivated in a short time, and through effective management in the process of planting the seedlings in the ground, the growth speeds and trunk shapes of the saplings are improved; rooting breeding can be performed on the branches to be pruned, of themiracle fruit plants, waste utilization is realized for raising the seedlings, the costs of raising the seedlings of the miracle fruits are reduced, meanwhile, the fruiting time of the miracle fruitsis shortened through the seedling raising method of layering, and the miracle fruits produce fruits in the current year or the following year; and the method has a wide application range.

The invention provides a mask with a function of preventing allergic asthma. The mask is characterized by comprising an inner layer and an outer layer, wherein a detachable traditional Chinese herbal medicine bag is further arranged between the inner layer and the outer layer; the traditional Chinese herbal medicine bag is filled with the following components in parts by weight: 8-10 parts of mint, 7-11 parts of violet magnolia, 14-16 parts of cocklebur fruit, 4-8 parts of ephedra, 6-9 parts of perilla leaves, 9-11 parts of ginger processed pinellia, 6-8 parts of liquorice, 7-9 parts of pogostemon cablin, 8-11 parts of perilla leaves and 8-10 parts of paris polyphylla. The traditional Chinese herbal medicine bag is arranged in the mask; body immunity can be improved through healthcare and stimulation effects of the traditional Chinese medicines; meanwhile, nasal mucosa is stimulated through aromatic odor of the traditional Chinese medicines, and the antibody content of the nasal mucosa is increased, so that the allergic asthma is prevented and treated.

The invention provides polyester fiber containing a wormwood component and wormwood fragrance and a preparation method of the polyester fiber, and relates to the technical field of textile fibers. The polyester fiber containing the wormwood component and the wormwood fragrance is prepared from the following materials in parts by weight of 8 to 11 parts of radix stemonae, 6 to 9 parts of flos caryophylli, 6 to 9 parts of herba taraxaci, 8 to 11 parts of radix et rhizoma rhei, 6 to 9 parts of flos gardeniae, 2 to 3 parts of rhizoma coptidis, 30 to 35 parts of wormwood, 5 to 7 parts of cellulase, 50 to 55 parts of polyester, 15 to 18 parts of lignans, 16 to 19 parts of nanoscale titanium dioxide and 25 to 28 parts of fiber-forming polymer. By adding a wormwood powder mixture and a wormwood extract, the taste of an industrial additive is effectively removed, meanwhile, the wormwood has the mosquito-repelling and insect-preventing effects, and secondly, the fragrance generated by the wormwood acts on nasal mucosa, so that the content of immune globulin S (SlGA) secreted by antibodies on the nasal mucosa is increased; and the increase of the content enables virus not to survive easily on the nasal mucosa and the respiratory mucosa.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine sachet for preventing viral infection flu. The sachet comprisesthe following components: notopterygium roots, rheum officinale, radix bupleuri, rhizoma atractylodis, asarum, folium artemisiae argyi and wrinkled gianthyssop herb, wherein the weight parts of the components are as follows: 3-5 parts of notopterygium roots, 3-5 parts of rheum officinale, 4-7 parts of radix bupleuri, 5-8 parts of rhizoma atractylodis, 6-10 parts of asarum, 6-10 parts of folium artemisiae argyi, and 5-8 parts of wrinkled gianthyssop herb. A preparation process comprises the following steps: S1, cleaning the components, drying the components in the sun under natural conditions,chopping the dried product by using a knife, and finally grinding the product into powder by using a grinder; S2, uniformly mixing the components according to the component ratio; S3, putting the uniformly mixed product into a traditional Chinese medicine bag, and sealing the bag with a plastic film to obtain a pure traditional Chinese medicine bag; and S4, bagging the pure traditional Chinese medicine bag into artwork ornaments to obtain the traditional Chinese medicine sachet.

The invention relates to a eucalyptus biochar loaded nano zero-valent iron composite material and a preparation method thereof. The preparation method specifically comprises the following steps: preparing eucalyptus biochar, preparing an extracting solution, carrying out modification treatment on the eucalyptus biochar, preparing a eucalyptus biochar loaded nano zero-valent iron solution and preparing the composite material. The eucalyptus biochar and the eucalyptus leaf extract are mixed and subjected to ultrasonic modification treatment, so that the number and the variety of surface functional groups are greatly increased, the agglomeration effect, the oxidized degree and the reaction performance can be effectively improved after the composite material is formed by loading the nano zero-valent iron, and the composite material has extremely high removal performance on a high-concentration Cr (VI) solution and is low in energy consumption, low in cost, safe and non-toxic; the efficient restoration of the water body is realized.