iPSC residue detection method using ESRG gene as universal marker gene

Abstract

The invention provides an iPSC residue detection method using ESRG gene as a universal marker gene. According to the iPSC residue detection method using the ESRG gene as the universal marker gene, thenumber of the ESRG genes in a sample is quantitatively detected through a qPCR detection method, so that iPSC residues in the sample are detected. According to the method, an ESRG gene segment is adopted as the universal marker gene, a series of cells including endothelial progenitor cells, neural stem cells, retinal pigment epitheliums, hepatocytes, natural killer cells, myocardial cells, isletbodies and the like can be detected, and the marker gene does not need to be replaced; and meanwhile, detection can be carried out in a short time, and the detection efficiency is greatly improved. According to the method, during detection, only mRNA extraction, reverse transcription and qPCR reagents are needed, the cost is low, and the accuracy is high.

Description

technical field [0001] The invention relates to an iPSC residual detection method using ESRG gene as a universal marker gene, belonging to the field of biotechnology. Background technique [0002] Induced pluripotent stem cells (iPSCs) are a type of pluripotent cells with self-renewal and self-replication capabilities. It can differentiate into all cells in the body, and then form all tissues and organs of the body. The advent of induced pluripotent stem cell (iPSC) technology has paved the way for regenerative medicine therapies. In theory, iPSCs can differentiate into any type of cell in our body, and scientists have developed various protocols to differentiate iPSCs into specific cell types, including nerve cells, cardiomyocytes, endothelial cells, retinal pigment epithelial cells, pancreatic islets bodies and hepatocytes, etc. In addition, iPSCs have also been widely used in organoid technology. [0003] iPSCs have the ability to proliferate indefinitely and can form...

AI Technical Summary

Problems solved by technology

But this approach is often targeted to specific functional cells and is not always applicable to functional cells

For example, studies have shown that lin28A cannot detect iPSC cell residues in iPSC-induced hepatocytes, endothelial progenitor cells, and islet bodies.

[0008] In addition, the detection methods in the prior art also have the disadvantages of low detection efficiency and high cost.

However, in the detection method expanded by cell culture, there are still problems that cells at too low a concentration are not easy to survive and iPSCs are easy to differentiate and cause false positives.

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