Multi-enzyme system used for separating patient tumor tissue-originated primary cells, application method thereof, purpose thereof, and relative kit thereof

Abstract

The invention relates to a multi-enzyme system used for separating patient tumor tissue-originated primary cells. The system comprises components of, by weight: 0.02 to 0.2% of collagenase I-IV, 0.001 to 0.01% of hyaluronidase, 0.01 to 0.1% of trypsin, 0.02 to 0.2% of proteinase neutral, 0.001 to 0.01% of deoxyribonuclease I, and 5 to 50U/ml of elastase. The invention also relates to an application method of the system, an application of the system, and a kit formed by the system. The multi-enzyme system is advantaged in reasonable design. With the system, patient tumor tissue-originated primary cells can be separated with high specificity and high sensitivity. The separated primary cells are characterized by high survival rate, high purity, easy cryopreservation and easy resuscitation. The system and the kit can be used in medicine experimental evaluation, medicine toxicity testing, and medicine therapeutic performance determination. The system, the method and the kit are suitable to be widely popularized.

Description

technical field [0001] The present invention relates to the technical field of primary cell separation, in particular to the technical field of tumor tissue-derived primary cell separation, and specifically refers to a multi-enzyme system for the separation of patient tumor tissue-derived primary cells, its use method, purpose and related reagents box. Background technique [0002] In the biomedical industry, especially the application industry of primary cell culture in drug research and development, the profit margin is large, the energy consumption is small, and the technical level is high. It is a policy support industry determined by the National Reform and Development Commission of China. Development Committee Guidance List of China's key development industries before 2010. Provincial governments and other local governments will support such projects as key technologies and major projects, and enjoy special preferential treatment in terms of manpower and financial res...

AI Technical Summary

Problems solved by technology

[0010] At present, in the research and development of drugs in the biomedical industry in the domestic and foreign markets, most of the research and development at the cell level uses tumor cell lines. reflect the biological characteristics and physiological functions of body tissues

The isolation and cultivation of tumor tissue-derived primary cells has always been a difficult point. The reason is that the application of cell digestive enzymes makes it difficult for isolated primary cells to survive in vitro.